Molecular-Based Identification of Sarcocystis hominis in Belgian Minced Beef

2007 ◽  
Vol 70 (6) ◽  
pp. 1523-1526 ◽  
Author(s):  
L. VANGEEL ◽  
K. HOUF ◽  
K. CHIERS ◽  
J. VERCRUYSSE ◽  
K. D'HERDE ◽  
...  
Keyword(s):  
18S Rrna ◽  
Intestinal Tract ◽  
Protozoan Parasite ◽  
Sarcocystis Species ◽  
Rrna Sequence ◽  
Specific Sequence ◽  
Sarcocystis Spp ◽  
Minced Beef ◽  
Pcr Products ◽  
Human Intestinal Tract

Sarcocystis hominis, one of the three species of Sarcocystis that cause muscular cysts in cattle, is a protozoan parasite that can infect the human intestinal tract. The objective of the present study was to develop a new molecular identification method capable of discriminating among the bovine Sarcocystis species and to apply this tool in combination with stereo-microscopy to determine the presence of Sarcocystis spp. in minced beef in Belgium, with special attention to Sarcocystis hominis. A PCR technique based on the 18S rRNA sequence and by sequencing of the amplicon was highly specific. Sequence analysis of PCR products from thick-walled cysts collected from minced beef in Belgium revealed that S. hominis was present in 97.4% of the samples. Because the consumption of raw minced beef is common in Belgium and certain other European countries, these findings may point to an underestimated risk to public health.

Unique report of two Sarcocystis species from Egyptian domestic chicken (Gallus gallus) – new host and locality

2021 ◽  
Vol 24 (1) ◽  
pp. 152-158
Author(s):  
E. M. Galila ◽  
E. K. A. Bazh ◽  
N. Elhawary ◽  
H. A. Abdellatif ◽  
A.-R. A. Abou-Rawash
Keyword(s):  
Nile Delta ◽  
Protozoan Parasite ◽  
Intestinal Wall ◽  
Sarcocystis Species ◽  
Middle Region ◽  
New Host ◽  
Sarcocystis Spp ◽  
Hexagonal Shape ◽  
The World ◽  
Intracellular Protozoan Parasite

Sarcocystis is an intracellular protozoan parasite in the phylum Apicomplexa. It is widely distributed all over the world. There are scarce reports about chicken Sarcocystis. From February 2016 to January 2018, a total number of 630 chicken carcasses, intestines and viscera were collected from different chicken markets in Menoufia and Gharbia Governorates, Middle region of the Nile Delta, Egypt and carefully inspected. Macroscopic and microscopic cysts of Sarcocystis spp. were found in the intestinal wall and mesentery of 5 birds. Histopathological sections revealed the presence of two shapes of the macroscopic cysts (oval and kidney shape). Their wall was striated and characterised by the presence of radial septa. It had compartments mostly of hexagonal shape, containing both bradyzoites and metrocytes in the periphery. The bradyzoites were banana-shaped and measured 20–30 × 8–10 μm with centrally or posteriorly located nuclei. Microscopic cysts of Sarcocystis spp. were detected in-between muscle bundles, with variable shapes (spindle and oval).

scholarly journals Genetic Diversity and Zoonotic Potential of Blastocystis in Korean Water Deer, Hydropotes inermis argyropus

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 955
Author(s):  
Kyoo-Tae Kim ◽  
Gyeonguk Noh ◽  
Haeseung Lee ◽  
Seon-Hee Kim ◽  
Hyesung Jeong ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Intestinal Tract ◽  
Protozoan Parasite ◽  
Domestic Animals ◽  
Nucleotide Sequencing ◽  
Zoonotic Potential ◽  
Infection Status ◽  
Korean Water Deer

Blastocystis is a protozoan parasite commonly detected in the intestinal tract of humans and animals. It has been actively studied worldwide; however, information on Blastocystis is limited in Korea. Because there is an increasing concern about the contact between wildlife and domestic animals or humans, we assessed the infection status and zoonotic potential of Blastocystis in Korean water deer (KWD, Hydropotes inermis argyropus) using genotyping and phylogenetic analysis. A total of 125 fresh fecal samples were collected from KWD which were killed by vehicles on highways or roadsides in this study. Among the 125 samples, 51 (40.8%) were PCR positive. We performed nucleotide sequencing and phylogenetic analysis of 26 of the 51 PCR-positive samples. By analyzing Blastocystis 18S rRNA, two subtypes (ST4 and ST14) were identified in this study. Of the 26 samples analyzed, 25 were identified as ST14 and one as ST4. Infection of ST14 in humans has not been reported. Although only one ST4 sample was detected in this study, ST4 has zoonotic potential without showing ruminant specificity. Thus, continuous attention should be provided to the potential of transmission between wildlife and domestic animals and humans.

Crosstalk between Entamoeba histolytica and the human intestinal tract during amoebiasis

Parasitology ◽  
2017 ◽  
Vol 146 (9) ◽  
pp. 1140-1149 ◽  
Author(s):  
Elisabeth Labruyère ◽  
Roman Thibeaux ◽  
Jean-Christophe Olivo-Marin ◽  
Nancy Guillén
Keyword(s):  
Entamoeba Histolytica ◽  
Intestinal Tract ◽  
Colonic Mucosa ◽  
Protozoan Parasite ◽  
Mortality Rates ◽  
High Morbidity ◽  
Mucus Layer ◽  
Lytic Enzymes ◽  
Microbial Agent ◽  
Human Intestinal Tract

AbstractThe protozoan parasite Entamoeba histolytica is the microbial agent of amoebiasis – an infection that is endemic worldwide and is associated with high morbidity and mortality rates. As the disease develops, virulent E. histolytica deplete the mucus layer, interact with the intestinal epithelium, and then degrade the colonic mucosa and disrupt the extracellular matrix (ECM). Our research demonstrated that virulent parasites with an invasive phenotype display rapid, highly specific changes in their transcriptome (notably for essential factors involved in carbohydrate metabolism and the processing of glycosylated residues). Moreover, combined activation of parasite and host lytic enzymes leads to the destruction of the intestinal parenchyma. Together, these enzymes degrade the mucus layer and the ECM, and trigger the inflammatory response essential to the development of amoebiasis.

scholarly journals Molecular and Microscopic Investigation of Sarcocystis Species Isolated from Sheep Muscles in Iran

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Nader Pestechian ◽  
Hossein Ali Yousefi ◽  
Reza Kalantari ◽  
Rasool Jafari ◽  
Faham Khamesipour ◽  
...  
Keyword(s):  
Distribution Patterns ◽  
18S Rrna Gene ◽  
Microscopic Investigation ◽  
Economic Losses ◽  
Rrna Gene ◽  
Sarcocystis Species ◽  
Digestion Method ◽  
Naked Eye ◽  
Sarcocystis Spp ◽  
Pcr Products

Sarcocystis species is a genus of cyst-forming parasites infecting both humans and animals globally. Some of these species cause clinical and subclinical diseases in the host and may lead to economic losses. This study was carried out to identify the distribution patterns of Sarcocystis spp. in slaughtered sheep based on the digestion method and PCR-RFLP in Isfahan, the center of Iran. In total, 150 fresh muscle samples (30 hearts, 60 esophagi, and 60 diaphragms) were investigated by naked eye observation and then scrutinized based on the digestion method. To this end, pepsin and HCl were used to observe the Sarcocystis parasite via a light microscope. The PCR was carried out to intensify a fragment of the 18S rRNA gene. Afterward, the PCR products were exposed to digestion by endonuclease TaqI, HindII, EcoRI, and AvaI. Consequently, the results of RFLP were confirmed by sequencing, and the phylogenetic placement of all species was analyzed. Through the examination by the naked eye, 5/150 (3.33%) macroscopic cysts were found in the samples. With the tissue digestion and microscopic examination, 116 (77.33%) samples were positive for Sarcocystis spp.; however, 125 (83.33%) samples were positive with PCR. Moreover, the results of sequence analysis on macrocysts and microcysts showed that 4% and 96% of the species belonged to S. gigantea and S. tenella, respectively. According to the results of the current study, sarcocystosis caused by S. tenella are highly prevalent among sheep in the Isfahan region. Due to the high prevalence of Sarcocystis infection in the world and Iran, the development of disease control and prevention policies in sheep would be essential, and changing attitudes in the way of keeping livestock from the traditional type to the industrial method is recommended in this regard.

The human intestinal tract – a hotbed of resistance gene transfer? Part I

2007 ◽  
Vol 29 (3) ◽  
pp. 17-21 ◽  
Author(s):  
Abigail A. Salyers ◽  
Kyung Moon ◽  
David Schlesinger
Keyword(s):  
Gene Transfer ◽  
Resistance Gene ◽  
Intestinal Tract ◽  
Human Intestinal Tract

scholarly journals In vitro processing at the 3'-terminal region of pre-18S rRNA by a nucleolar endoribonuclease.

1990 ◽  
Vol 10 (8) ◽  
pp. 3868-3872 ◽  
Author(s):  
C M Shumard ◽  
C Torres ◽  
D C Eichler
Keyword(s):  
18S Rrna ◽  
Precise Determination ◽  
In Vivo Studies ◽  
Rrna Sequence ◽  
S1 Nuclease ◽  
In Vitro Processing ◽  
Rrna Maturation ◽  
A Site

In an investigation of the possible involvement of a highly purified nucleolar endoribonuclease in processing of pre-rRNA at the 3' end of the 18S rRNA sequence, an in vitro synthesized pre-18S rRNA transcript containing the 3' end region of 18S rRNA and the 5' region of the first internal transcribed spacer (ITS1) was used as a substrate for the enzyme. Cleavages generated by the nucleolar RNase were localized by S1 nuclease protection analysis and by the direct release of labeled rRNA products. Precise determination of the specificity of cleavage was achieved by RNA sequence analysis with end-labeled rRNA transcripts. These data demonstrated that the purified nucleolar RNase cleaved the pre-18S rRNA transcript at three specific sites relative to the 3' region of 18S rRNA. The first two sites included the mature 3'-end 18S rRNA sequence and a site approximately 55 nucleotides downstream of the 3'-end 18S rRNA sequence, both of which corresponded directly to recent results (Raziuddin, R. D. Little, T. Labella, and D. Schlessinger, Mol. Cell. Biol. 9:1667-1671, 1989) obtained with transfected mouse rDNA in hamster cells. The other cleavage occurred approximately 35 nucleotides upstream from the mature 3' end in the 18S rRNA sequence. The results from this study mimic the results obtained from in vivo studies for processing in the 3' region of pre-18S rRNA, supporting the proposed involvement of this nucleolar endoribonuclease in rRNA maturation.

Three new small nucleolar RNAs that are psoralen cross-linked in vivo to unique regions of pre-rRNA

1993 ◽  
Vol 13 (7) ◽  
pp. 4382-4390
Author(s):  
O J Rimoldi ◽  
B Raghu ◽  
M K Nag ◽  
G L Eliceiri
Keyword(s):  
Small Rnas ◽  
18S Rrna ◽  
Rnase H ◽  
Antisense Oligodeoxynucleotide ◽  
28S Rrna ◽  
Small Nucleolar Rnas ◽  
Rrna Sequence ◽  
Nucleolar Rna ◽  
Nucleolar Rnas

We have recently described three novel human small nucleolar RNA species with unique nucleotide sequences, which were named E1, E2, and E3. The present article describes specific psoralen photocross-linking in whole HeLa cells of E1, E2, and E3 RNAs to nucleolar pre-rRNA. These small RNAs were cross-linked to different sections of pre-rRNA. E1 RNA was cross-linked to two segments of nucleolar pre-rRNA; one was within residues 697 to 1163 of the 5' external transcribed spacer, and the other one was between nucleotides 664 and 1021 of the 18S rRNA sequence. E2 RNA was cross-linked to a region within residues 3282 to 3667 of the 28S rRNA sequence. E3 RNA was cross-linked to a sequence between positions 1021 and 1639 of the 18S rRNA sequence. Primer extension analysis located psoralen adducts in E1, E2, and E3 RNAs that were enriched in high-molecular-weight fractions of nucleolar RNA. Some of these psoralen adducts might be cross-links of E1, E2, and E3 RNAs to large nucleolar RNA. Antisense oligodeoxynucleotide-targeted RNase H digestion of nucleolar extracts revealed accessible segments in these three small RNAs. The accessible regions were within nucleotide positions 106 to 130 of E1 RNA, positions 24 to 48 and 42 to 66 of E2 RNA, and positions 7 to 16 and about 116 to 122 of E3 RNA. Some of the molecules of these small nucleolar RNAs sedimented as if associated with larger structures when both nondenatured RNA and a nucleolar extract were analyzed.

scholarly journals Emergence of Babesia Conradae Infection in Coyote-hunting Greyhounds in Oklahoma, USA

2021 ◽  
Author(s):  
Erin M Stayton ◽  
Megan Lineberry ◽  
Jennifer Thomas ◽  
Tina Bass ◽  
Kelly Allen ◽  
...  
Keyword(s):  
18S Rrna ◽  
Clinical Signs ◽  
18S Rrna Gene ◽  
Post Treatment ◽  
Rrna Gene ◽  
Babesia Gibsoni ◽  
Wide Range ◽  
Pcr Products ◽  
Life Threatening ◽  
Tick Vectors

Abstract Background: Babesia species are intraerythrocytic Apicomplexan parasites that infect a wide range of vertebrate hosts. These pathogens are typically transmitted either by tick vectors or by direct blood-to-blood contact, and may cause life-threatening clinical disease such as thrombocytopenia, hemolytic anemia, and acute renal failure in canine hosts. While Babesia vogeli and Babesia gibsoni infections have both been reported in Oklahoma, reports of B. conradae infections have been limited to California. Methods: Whole blood samples were collected in EDTA tubes from all dogs in four separate kennels in Oklahoma. DNA was extracted from each blood sample and a nested PCR was performed using general Apicomplexan primers for the partial 18S rRNA gene. PCR products were electrophoresed in agarose matrix and appropriately sized amplicons were sequenced. Sequences were compared to reference 18S rRNA sequences available in GenBank, and samples with >98% homology to B. conradae (GenBank MK256976) were considered positive. B. conradae positive dogs were then treated with atovaquone (13.5 mg/kg TID) and azithromycin (10 mg/kg SID) for 10 days and retested at 30 and 60 days post treatment by PCR. Results: Fifteen of 40 dogs tested positive for B. conradae with 98–100% sequence homology to B. conradae from California. All positive cases were coyote-hunting Greyhounds. Treatment of clinically ill dogs with atovaquone and azithromycin resulted in complete clinical recovery in clinically ill dogs and all treated dogs had negative follow-up PCR at 30 and 60 days post treatment. Conclusions: Collectively, this study (i) documents the occurrence of B. conradae in Oklahoma, (ii) highlights this pathogen as a differential to be considered when clinical signs are present, and (iii) supports the use of atovaquone and azithromycin as effective treatment in these cases.

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