scholarly journals Mutagenic and recombinogenic assessment of widely used pesticides on Drosophila melanogaster

2019 ◽  
Vol 22 (2) ◽  
pp. 200-212
Author(s):  
R. Sarıkaya ◽  
B. Koçak Memmi ◽  
S. Sümer ◽  
F. Erkoç
Keyword(s):  
Drosophila Melanogaster ◽  
Somatic Cells ◽  
Imaginal Discs ◽  
Spot Test ◽  
Genotoxic Effects ◽  
Mutagenic Potential ◽  
Genetic Changes ◽  
The Third ◽  
Drosophila Wing ◽  
High Doses

The mutagenic potential of selected widely used pesticides: p,p'-dichlorodiphenyltrichloroethane (DDT); fenitrothion; propoxur; deltamethrin, bifenthrin; imidacloprid and thiametoxam was assessed using the wing spot test. Third-instar larvae of standard Drosophila melanogaster cross (ST), trans-heterozygous for the third chromosome recessive markers, multiple wing hairs (mwh) and flare (flr3) were chronically exposed to test compounds. Feeding ended with pupation of the surviving larvae. Genetic changes induced in somatic cells of the wing’s imaginal discs, mutant spots observed in marker-heterozygous (MH) and balancer-heterozygous (BH) flies were compared using the wing spot test, to estimate the genotoxic effects of these pesticides. In conclusion, exposure to 30 mg/mL deltamethrin, 40 mg/mL imidacloprid, 100 µg/mL DDT showed mutagenic and recombinagenic effects in the Drosophila wing spot test. In addition the results of chronic treatments performed at high doses showed mutagenic and recombinagenic effects in both genotypes

scholarly journals Interactions and developmental effects of mutations in the Broad-Complex of Drosophila melanogaster.

Genetics ◽  
1988 ◽  
Vol 118 (2) ◽  
pp. 247-259
Author(s):  
I Kiss ◽  
A H Beaton ◽  
J Tardiff ◽  
D Fristrom ◽  
J W Fristrom
Keyword(s):  
Drosophila Melanogaster ◽  
X Chromosome ◽  
Genetic Locus ◽  
Larval Instar ◽  
Imaginal Discs ◽  
Functional Domains ◽  
Developmental Processes ◽  
The Third ◽  
Developmental Effects ◽  
Broad Complex

Abstract The 2B5 region on the X chromosome of Drosophila melanogaster forms an early ecdysone puff at the end of the third larval instar. The region contains a complex genetic locus, the Broad-Complex (BR-C) composed of four groups of fully complementing (br, rbp, l(1)2Bc, and l(1)2Bd) alleles, and classes of noncomplementing (npr 1) and partially noncomplementing l(1)2Bab alleles. BR-C mutants prevent metamorphosis, including the morphogenesis of imaginal discs. Results are presented that indicate that the BR-C contains two major functional domains. One, the br domain is primarily, if not exclusively, involved in the elongation and eversion of appendages by imaginal discs. The second, the l(1)2Bc domain, is primarily involved in the fusion of discs to form a continuous adult epidermis. Nonetheless, the two domains may encode products with related functions because in some situations mutants in both domains appear to affect similar developmental processes.

Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron

1991 ◽  
Vol 11 (3) ◽  
pp. 1538-1546
Author(s):  
A C Chain ◽  
S Zollman ◽  
J C Tseng ◽  
F A Laski
Keyword(s):  
Drosophila Melanogaster ◽  
Splice Site ◽  
Germ Line ◽  
Somatic Cells ◽  
Regulatory Region ◽  
Systematic Search ◽  
P Element ◽  
Regulatory Sequence ◽  
Cis Acting ◽  
The Third

P element transposition in Drosophila melanogaster is limited to the germ line because the third intron (the ORF2-ORF3 intron) of the P element transcript is spliced only in germ line cells. We describe a systematic search for P element sequences that are required to regulate the splicing of the ORF2-ORF3 intron. We have identified three adjacent mutations that abolish the germ line specificity and allow splicing of this intron in all tissues. These mutations define a 20-base regulatory region located in the exon, 12 to 31 bases from the 5' splice site. Our data show that this cis-acting regulatory sequence is required to inhibit the splicing of the ORF2-ORF3 intron in somatic cells.

scholarly journals Genotoxicity assessment of Schinopsis brasiliensis Engl. (Sapindales: Anacardiaceae) in somatic cells of Drosophila melanogaster (Meigen, 1830) (Diptera: Drosophilidae)

2018 ◽  
Vol 5 (10) ◽  
pp. 471-479
Author(s):  
Silmara de Moraes Pantaleão ◽  
Jéssica Adele dos Santos ◽  
Thalyta Linacher ◽  
Clisiane Carla de Souza Santos ◽  
Charles dos Santos Estevam ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Ethyl Acetate ◽  
Plant Species ◽  
Somatic Mutation ◽  
Folk Medicine ◽  
Somatic Cells ◽  
Ethyl Acetate Fraction ◽  
Genotoxic Effects ◽  
Medicinal Use ◽  
Phytochemical Profile

Schinopsis brasiliensis Engl. (Sapindales: Anacardiaceae) has been used in the Brazilian folk medicine to treat several illnesses. However, the phytochemical profile of S. brasiliensis as well as its genotoxic potential are poorly understood, which compromises population safety regarding the medicinal use of this plant species. In this study, we analyzed the genotoxic effects of S. brasiliensis using the Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster. Larvae from both standard cross (ST) and high bioactivation capacity cross (HB) were exposed to different concentrations of the hydroetanolic extract and ethyl acetate fraction of S. brasiliensis. We analyzed wings from D. melanogaster according to the type and number of mutant hair. Ours results suggested no genotoxic activity of S. brasiliensis in D. melanogaster somatic cells.

scholarly journals Mutagenic potential and modulatory effects of the medicinal plant Luehea divaricata (Malvaceae) in somatic cells of Drosophila melanogaster: SMART/wing

2011 ◽  
Vol 10 (1) ◽  
pp. 16-24 ◽  
Author(s):  
L.P. Fel�cio ◽  
E.M. Silva ◽  
V. Ribeiro ◽  
C.T. Miranda ◽  
I.L.B.F. Vieira ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Medicinal Plant ◽  
Somatic Cells ◽  
Mutagenic Potential

scholarly journals Genotoxicity of Copper and Nickel Nanoparticles in Somatic Cells of Drosophila melanogaster

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Erico R. Carmona ◽  
Alba García-Rodríguez ◽  
Ricard Marcos
Keyword(s):  
Drosophila Melanogaster ◽  
Laser Doppler Velocimetry ◽  
Antimicrobial Agents ◽  
Nickel Nanoparticles ◽  
Somatic Cells ◽  
Industrial Applications ◽  
Electronic Microscopy ◽  
Genotoxic Effects

Copper and nickel nanoparticles (Cu-NPs and Ni-NPs, respectively) are used in a variety of industrial applications, such as semiconductors, catalysts, sensors, and antimicrobial agents. Although studies on its potential genotoxicity already exist, few of them report in vivo data. In the present study we have used the wing-spot assay in Drosophila melanogaster to determine the genotoxic activity of Cu-NPs and Ni-NPs, and these data have been compared with those obtained with their microparticle forms (MPs). Additionally, a complete physical characterization of NPs using transmission electronic microscopy (TEM), dynamic light scattering (DLS), and laser Doppler velocimetry (LDV) techniques was also performed. Results obtained with Cu-NPs and Cu-MPs indicate that both failed to induce an increase in the frequency of mutant spots formation in the wings of the adults, suggesting a lack of genotoxicity in somatic cells of D. melanogaster. However, when Ni-NPs and Ni-MPs were evaluated, a significant increase of small single spots and total mutant spots was observed only for Ni-NPs (P<0.05) at the highest dose assessed. Thus, the genotoxicity of Ni-NPs seem to be related to their nanoscale size, because no genotoxic effects have been reported with their microparticles and ions. This study is the first assessing the in vivo genotoxic potential of Cu-NPs and Ni-NPs in the Drosophila model.

scholarly journals Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron.

1991 ◽  
Vol 11 (3) ◽  
pp. 1538-1546 ◽  
Author(s):  
A C Chain ◽  
S Zollman ◽  
J C Tseng ◽  
F A Laski
Keyword(s):  
Drosophila Melanogaster ◽  
Splice Site ◽  
Germ Line ◽  
Somatic Cells ◽  
Regulatory Region ◽  
Systematic Search ◽  
P Element ◽  
Regulatory Sequence ◽  
Cis Acting ◽  
The Third

P element transposition in Drosophila melanogaster is limited to the germ line because the third intron (the ORF2-ORF3 intron) of the P element transcript is spliced only in germ line cells. We describe a systematic search for P element sequences that are required to regulate the splicing of the ORF2-ORF3 intron. We have identified three adjacent mutations that abolish the germ line specificity and allow splicing of this intron in all tissues. These mutations define a 20-base regulatory region located in the exon, 12 to 31 bases from the 5' splice site. Our data show that this cis-acting regulatory sequence is required to inhibit the splicing of the ORF2-ORF3 intron in somatic cells.

Evaluation of the mutagenic potential of Tepezcohuite in the Drosophila wing spot test

1991 ◽  
Vol 264 (3) ◽  
pp. 115-116 ◽  
Author(s):  
A.Emilio Pimentel P. ◽  
Martha P. Cruces M. ◽  
Stanley Zimmering
Keyword(s):  
Spot Test ◽  
Mutagenic Potential ◽  
Drosophila Wing

Mutagenicity of 5,6-Dihydropenicillic Acid (DHPA) in Drosophila melanogaster and Bacterial Conversion of Penicillic Acid to DHPA

1995 ◽  
Vol 58 (12) ◽  
pp. 1375-1378 ◽  
Author(s):  
H. OBANA ◽  
Y. KUMEDA ◽  
T. NISHIMUNE
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drosophila Melanogaster ◽  
Yeast Extract ◽  
Spot Test ◽  
Crossing Over ◽  
Penicillic Acid ◽  
Drosophila Wing ◽  
Spoilage Bacteria ◽  
Chromosome Recombination ◽  
Environmental Bacteria

We studied the mutagenicity of 5,6-dihydropenicillic acid (DHPA) by means of the Drosophila wing-spot test. DHPA (10 mg/g of medium) significantly increased the number of small single and twin spots. Since twin spots were exclusively caused by mitotic crossing-over, the results demonstrated that DHPA can induce chromosome recombination in Drosophila somatic cells. Penicillic acid (PA) was converted to DHPA by an incubation with Agrobacterium radiobactor. The conversion occurred with other spoilage bacteria, such as Pseudomonas aeruginosa and P. cepacia. PA was completely converted to DHPA within a 24-h incubation with P. aeruginosa or P. cepacia in medium containing yeast extract. The results suggested that mutagenic DHPA is produced by environmental bacteria when foods are contaminated by fungi which produce PA.

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