scholarly journals Pathological and Immunological Study of an in ovo Complex Vaccine against Infectious Bursal Disease

2000 ◽  
Vol 48 (4) ◽  
pp. 443-454 ◽  
Author(s):  
Mária Kelemen ◽  
Katalin Forgách ◽  
Judit Iván ◽  
V. Palya ◽  
T. Süveges ◽  
...  
Keyword(s):  
Newcastle Disease ◽  
Broiler Chicken ◽  
Haemagglutination Inhibition ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Hatching Rate ◽  
Immunological Study ◽  
In Ovo ◽  
Chicken Embryos ◽  
Bursal Disease

The appearance of very virulent strains of infectious bursal disease (IBD) virus at the end of the 1980s made it necessary to develop more effective immunization procedures. To facilitate this, the immunogenicity and the immunosuppressive effect of a mild (G-87), an intermediate (LIBD) and an intermediate-plus (IBDV 2512) IBDV strain were tested after the in ovo inoculation of 18-day-old SPF and broiler chicken embryos. It was established that no noteworthy difference existed between the immunized and the control embryos in hatching rate and hatching weight. The higher the virulence of the vaccine virus strain, the more severe damage it caused to the lymphocytes of the bursa of Fabricius. In SPF chickens, the haemagglutination inhibition (HI) titres induced by a Newcastle disease (ND) vaccine administered at day old decreased in inverse ratio to the virulence of the IBD vaccine strain, while in broiler chickens this was not observed. Despite the decrease of the HI titre, the level of protection did not decline, or did so only after the use of the ‘hot’ strain. SPF chickens immunized in ovo with a complex vaccine prepared from strain IBDV 2512 and IBD antibody showed the same protection against Newcastle disease as the broilers. In broiler chicken embryos immunized in ovo, only strain IBDV 2512 induced antibody production, and such chickens were protected against IBD at 3 weeks of age. The complex vaccine administered in ovo has been used successfully at farm hatcheries as well.

Novel in-ovo chimeric recombinant Newcastle disease vaccine protects against both Newcastle disease and infectious bursal disease

Vaccine ◽  
2014 ◽  
Vol 32 (13) ◽  
pp. 1514-1521 ◽  
Author(s):  
Jinying Ge ◽  
Xijun Wang ◽  
Meijie Tian ◽  
Zhiyuan Wen ◽  
Qiulin Feng ◽  
...  
Keyword(s):  
Newcastle Disease ◽  
Infectious Bursal Disease ◽  
In Ovo ◽  
Bursal Disease ◽  
Newcastle Disease Vaccine

scholarly journals Serological Survey of Dominant Viral Diseases (Newcastle Disease (ND), Infectious Bronchitis (IB) and Infectious Bursal Disease (IBD)), in Broilers Flocks in Northern Algeria

2018 ◽  
Vol 75 (2) ◽  
pp. 155
Author(s):  
Omar SALHI ◽  
Djemel KHELEF ◽  
Chafik- Reda MESSAI ◽  
Aziz LOUNAS ◽  
Abdellah MOHAMED-CHERIF ◽  
...  
Keyword(s):  
Risk Factors ◽  
Newcastle Disease ◽  
Broiler Chicken ◽  
Infectious Bursal Disease ◽  
Viral Diseases ◽  
Serological Survey ◽  
Elisa Method ◽  
Infectious Bronchitis ◽  
Bursal Disease ◽  
Good Hygiene

The present study was conducted to survey about sero-epidemiological status of Newcastle disease (ND), Infectious bronchitis (IB) and Infectious bursal disease (IBD) on Algerian broiler chicken (30 flocks/1200 sera) using ELISA method and to assess the influence of some risk factors related to each disease. Among all investigated flocks, ND was the most seroprevalent disease (63.33%); however, IB and IBD showed less serological positivity (40% and 16.66% respectively). For ND, Cobb 500 Flocks were significantly more seropositive by 78% (p = 0.025) than other strains. Nevertheless, flocks with good hygiene were significantly less seropositive to ND by 26% (p = 0.022). For IB, the risk of seropositivity was significantly lower in spring by 40% (p = 0.036). Although, flocks with higher density or with more than 30 days old were more seropositive respectively by 47% (p = 0.041) and 45% (p = 0.019). At last, when broiler chicken were not boosted by IBD vaccine, flocks appeared to be more seropositive by 48% (p = 0.047); especially in spring by 45% (p = 0.048); or in farms with poor hygiene by 65% (p = 0.004); however, more than flocks 30 days old flocks were less seropositive by 30% (p = 0.009).

scholarly journals Occurrence of Newcastle Disease and Infectious Bursal Disease Virus Antibodies in Double-Spurred Francolins in Nigeria

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Daniel Oladimeji Oluwayelu ◽  
Adebowale Idris Adebiyi ◽  
Ibukunoluwa Olaniyan ◽  
Phyllis Ezewele ◽  
Oluwasanmi Aina
Keyword(s):  
Newcastle Disease ◽  
Haemagglutination Inhibition ◽  
Infectious Bursal Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Southwestern Nigeria ◽  
Bursal Disease ◽  
Intensive Rearing ◽  
Live Bird ◽  
Apparently Healthy

The double-spurred francolin Francolinus bicalcaratus has been identified as a good candidate for future domestication due to the universal acceptability of its meat and its adaptability to anthropogenically altered environments. Therefore, in investigating the diseases to which they are susceptible, serum samples from 56 francolins in a major live-bird market (LBM) in Ibadan, southwestern Nigeria, were screened for antibodies against Newcastle disease (ND) and infectious bursal disease (IBD) viruses. Haemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay (ELISA) revealed 25.0% and 35.7% prevalence of ND virus (NDV) antibodies, respectively, while 5.4% and 57.1% prevalence of IBD virus (IBDV) antibodies was detected by agar gel precipitation test (AGPT) and ELISA, respectively. This first report on the occurrence of NDV and IBDV antibodies in apparently healthy, unvaccinated double-spurred francolins from a LBM suggests that they were subclinically infected with either field or vaccine viruses and could thus serve as possible reservoirs of these viruses to domestic poultry. Furthermore, if they are to be domesticated for intensive rearing, a vaccination plan including ND and IBD should be developed and implemented.

scholarly journals Isolation of Infectious Bursal Disease Virus Using Indigenous Chicken Embryos in Kenya

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
W. U. Mutinda ◽  
L. W. Njagi ◽  
P. N. Nyaga ◽  
L. C. Bebora ◽  
P. G. Mbuthia ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Economic Activities ◽  
Indigenous Chicken ◽  
Chicken Embryos ◽  
Local Resources ◽  
Bursal Disease ◽  
Specific Pathogen

Infectious bursal disease virus (IBDV) isolates were recovered from outbreaks to initiate activities towards developing a local vaccine strain. Use of indigenous chicken embryos was exploited to determine their potential, promote utilization of local resources for research, and enhance household economic activities. Bursa of Fabricius (BFs) samples from outbreaks shown to be IBDV positive was homogenized and inoculated in 4-week-old specific pathogen-free (SPF) IBDV seronegative white leghorn chicks. The harvested virus was inoculated into 11-day-old indigenous chicken embryos that were IBDV seronegative and passaged serially three times after which they were inoculated into 4-week-old indigenous chicks to test for presence and virulence of propagated virus. Out of 153 BFs collected from outbreaks, 43.8% (67/153) were positive for IBDV antigen and 65.7% (44/67) caused disease in SPF chicks. The embryo mean mortalities were 88% on primary inoculation, 94% in 1st passage, 91% in 2nd passage, and 67% in 3rd passage. After the third passage in embryos all the 44 isolates were virulent in 4-week-old indigenous chicks. The results show that indigenous chicken embryos support growth of IBDV and can be used to propagate the virus as an alternative viral propagating tool for respective vaccine preparation.

scholarly journals Epidemiology of infectious bursal disease in broiler birds of three districts in Bangladesh

2015 ◽  
Vol 1 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Shovon Chakma
Keyword(s):  
Broiler Chicken ◽  
Winter Season ◽  
Cross Sectional Study ◽  
Current Control ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Cross Sectional ◽  
High Prevalence ◽  
Bursal Disease ◽  
The Difference

A cross-sectional study was conducted in 450 broiler farms of three districts of Bangladesh from June 2012 to July 2013. The estimated prevalence and mortality of infectious bursal disease (IBD) in three districts were 10.2% (CI, 9.2-11.2) and 7.8% (CI, 6.4-9.2) respectively. On clinical sign such as anorexia, ruffled feathers and diarrhoea and necropsy gross changes at bursa of fabricius, the highest prevalence was recorded in Comilla district (10.4%) followed by Feni district (10.3%) and Chittagong district (10.0%), while the highest mortality was recorded in the same Comilla district (8.3%) followed by Chittagong district (8.1%) and Feni district (7.0%). IBD was recorded year-round and had significant association (P<0.05) with season. Significantly higher prevalence (13.1%) and mortality (9.8%) was documented in winter season. Broiler chicken age between 21-30 days was significantly reported with high prevalence rate and high mortality rate. The study result indicated the difference of IBD occurrence in Bangladesh, thereby would support to improve the current control plans.Asian J. Med. Biol. Res. June 2015, 1(1): 59-64

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