Assimilation of various organic carbon sources by Haematococcus strains

2009 ◽  
Vol 57 (2) ◽  
pp. 231-237
Author(s):  
M. Zych ◽  
A. Stolarczyk ◽  
K. Maca ◽  
A. Banaś ◽  
K. Termińska-Pabis ◽  
...  

Differences in the assimilation of individual organic compounds (5 mM sugars and L-asparagine) under mixotrophic growth conditions were described for three naturally occurring Haematococcus strains.The effects of assimilation were measured by the growth intensity and size of algal cells, and the effect of colour changes in the cultures was observed. Some compounds caused the cell colouration to change from green to yellow, being the result of chlorophyll disappearance and the accumulation of yellow secondary carotenoids. In the present experiment none of the cultures turned red, thus excluding the intense accumulation of the commercially interesting carotenoid, astaxanthin.

2012 ◽  
Vol 610-613 ◽  
pp. 1789-1794
Author(s):  
Wei Hua Xu ◽  
Yun Guo Liu ◽  
Guang Ming Zeng ◽  
Xin Li ◽  
Wei Zhang

The effect of different organic carbon sources on Cr(Ⅵ) reduction by Bacillus sp. was investigated. Three organic compounds, malate, succinate and glucose were tested in Cr(Ⅵ) reduction experiments. The results demonstrated that three organic compounds all significantly enhanced Cr(Ⅵ) reduction. The enhancing degree of these three organic compounds was observed as malate>glucose>succinate, although the cell growth in succinate-added medium was similar to that in malate-added medium and was apparently higher than that in glucose-added medium. Addition of glucose resulted in decrease of pH and increase of redox potential, while addition of malate or succinate resulted in increase of pH and decrease of redox potential during Cr(Ⅵ) reduction. Cr(Ⅵ) was reduced by liquid culture metabolite when glucose was added in liquid medium, but not reduced by liquid culture metabolite when malate or succinate was applied in liquid medium for bacterial cultivation.


2007 ◽  
Vol 20-21 ◽  
pp. 469-472
Author(s):  
Anna E. Zhuravleva ◽  
I.A. Tsaplina ◽  
A.D. Ismailov ◽  
L.M. Zakharchuk ◽  
G.I. Karavaiko

The genus Sulfobacillus includes moderately thermophilic and thermotolerant acidophilic bacteria, which prefer mixotrophic growth conditions. These organisms are S. thermosulfidooxidans 1269Т, S. sibiricus N1Т and S. thermotolerans Kr1Т. The simultaneous presence of organic (for example yeast extract) and inorganic energy sources is essential for optimal growth. Carbon sources are both CO2 and organic compounds. During their mixotrophic growth, the basic pathways of carbohydrate catabolism of sulfobacilli are those of glycolysis, oxidative pentose phosphate and Entner–Doudorov (excepting strain N1). Assimilation of CO2 is carried out both auto- and heterotrophically. Final stages of oxidation of organic substances serve for cell biosynthesis. Bacteria are able to switch to organo- and autotrophic metabolism. At that the enzyme activities of pentose phosphate pathway are not detected. The cycle of tricarboxylic acids is disrupted at the level of 2-oxoglutarate dehydrogenase. The glyoxylate bypass is absent. The maximal protein and ATP values and high intensity of respiration in S. thermosulfidooxidans, S. sibiricus and S. thermotolerans cultures were determined under mixotrophic conditions. Studied strains due to their flexible carbon and energetic metabolism together with other microorganisms of communities (leptospirilli and archaea) participate in sulfide minerals processing at 40-450C without organic compounds supplement.


2017 ◽  
Vol 35 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Krista M. A. Paulson ◽  
Carol J. Ptacek ◽  
David W. Blowes ◽  
W. Douglas Gould ◽  
Jing Ma ◽  
...  

2014 ◽  
Vol 122 (2-3) ◽  
pp. 361-374 ◽  
Author(s):  
J. J. Dick ◽  
D. Tetzlaff ◽  
C. Birkel ◽  
C. Soulsby

2019 ◽  
Vol 15 (8-9) ◽  
pp. 513-522 ◽  
Author(s):  
Jianguo Du ◽  
Zehao Chen ◽  
Meiling Xie ◽  
Mingru Chen ◽  
Xinqing Zheng ◽  
...  

2000 ◽  
Vol 66 (10) ◽  
pp. 4518-4522 ◽  
Author(s):  
N. D. Gray ◽  
R. Howarth ◽  
R. W. Pickup ◽  
J. Gwyn Jones ◽  
I. M. Head

ABSTRACT Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of theAchromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [14C]bicarbonate and [14C]acetate. This extends previous findings thatAchromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).


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