Effect of supplementation with methionine and different fat sources on the glutathione redox system of growing chickens

2004 ◽  
Vol 52 (3) ◽  
pp. 369-378 ◽  
Author(s):  
Katalin Németh ◽  
M. Mézes ◽  
T. Gaál ◽  
Á. Bartos ◽  
K. Balogh ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Reduced Glutathione ◽  
Redox System ◽  
Corn Germ ◽  
Postnatal Life ◽  
Glutathione Peroxidase Activity ◽  
Glutathione Redox System ◽  
Corn Germ Oil ◽  
Glutathione Redox

The effect of supplementary methionine and fats of different saturation levels on the glutathione redox system of growing broiler cockerels was studied. The diet of three groups of chicks was supplemented with corn germ oil, beef tallow and fish oil at the levels of 30 g/kg and 50 g/kg of feed, respectively. The diet of further three groups was supplemented with methionine (5 g/kg of feed) in addition to the different fat sources. Control chicks were fed with a compound feed without methionine and fat supplementation. Reduced glutathione (GSH) and glutathione disulphide (GSSG) content as well as glutathione peroxidase activity in the liver were determined and GSH/GSSG ratio was calculated at day old and then at one and three weeks of age. Our results indicate that supplementary methionine stimulates both the synthesis of the glutathione redox system and glutathione peroxidase activity in growing chickens in the first period of postnatal life, when the risk of lipid peroxidation is high due to feeding unsaturated fats in the diet.

Effects of ochratoxin a on some production traits, lipid peroxide and glutathione redox status of weaned piglets

2007 ◽  
Vol 55 (4) ◽  
pp. 463-470 ◽  
Author(s):  
K. Balogh ◽  
J. Hausenblasz ◽  
Mária Weber ◽  
Márta Erdélyi ◽  
Judit Fodor ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Glutathione Peroxidase ◽  
Blood Plasma ◽  
Peroxidase Activity ◽  
Redox System ◽  
Production Traits ◽  
Weaned Piglets ◽  
Glutathione Peroxidase Activity ◽  
Glutathione Redox System ◽  
Glutathione Redox

The effect of feeding ochratoxin A (OTA) contaminated diet (379.6 and 338.1 μg/kg in starter and grower diets) on production traits, lipid peroxidation and some parameters of the glutathione redox system were investigated in weaned piglets over a seven-week period. Feed intake and feed conversion ratio (FCR) did not differ significantly, but in the first phase (0–28 days) the daily weight gain was significantly lower in the piglets fed the OTA-contaminated diet. Lipid peroxidation, as measured by the amount of malondialdehyde, glutathione content and glutathione peroxidase activity, did not change significantly in the blood plasma and red blood cell haemolysate in the OTA-loaded group, while malondialdehyde content increased significantly in the liver and markedly but not significantly in the kidney of piglets fed OTA-contaminated feed. Glutathione content did not differ significantly in the studied organs of the two groups while glutathione peroxidase activity of the OTA-loaded animals was significantly lower both in the liver and in the kidney. The results suggest that the use of feed-stuffs contaminated with low levels of OTA for seven weeks did not cause marked differences in the production traits or in lipid peroxidation and amount or activity of the glutathione redox system in the blood plasma, red blood cells and kidney, while significant changes occurred in the liver homogenate.

scholarly journals Role of glutathione redox system on the susceptibility to deoxynivalenol of pheasant (Phasianus colchicus)

2019 ◽  
Vol 36 (2) ◽  
pp. 175-182 ◽  
Author(s):  
Csaba Fernye ◽  
Zsolt Ancsin ◽  
Márta Erdélyi ◽  
Miklós Mézes ◽  
Krisztián Balogh
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Blood Plasma ◽  
Reduced Glutathione ◽  
Redox System ◽  
Phasianus Colchicus ◽  
Glutathione Peroxidase Activity ◽  
Glutathione Redox System ◽  
Dose Dependent ◽  
Glutathione Redox

AbstractThere are only a few reports on the effects of mycotoxins on pheasant (Phasianus colchicus) and the susceptibility to deoxynivalenol of these birds have never been reported before. The present experiment focuses to investigate the effects of different dietary concentrations of deoxynivalenol on blood plasma protein content, some parameters of lipid peroxidation and glutathione redox system and on the performance of pheasant chicks. A total of 320 1-day-old female pheasants were randomly assigned to four treatment groups fed with a diet contaminated with deoxynivalenol (control, 5.11 mg/kg, 11.68 mg/kg and 16.89 mg/kg). Birds were sacrificed at early (12, 24 and 72 h) and late (1, 2 and 3 weeks) stages of the experiment to demonstrate the oxidative stress-inducing effect of deoxynivalenol. Feed refusal was dose dependent, especially in the last third of the trial, but only minor body weight gain decrease was found. Lipid-peroxidation parameters did not show dose-dependent effect, except in blood plasma during the early stage of the trial. The glutathione redox system, reduced glutathione content and glutathione peroxidase activity, was activated in the liver, but primarily in the blood plasma. Glutathione peroxidase activity has changed parallel with reduced glutathione concentration in all tissues. Comparing our results with literature data, pheasants seem to have the same or higher tolerance to deoxynivalenol than other avian species, and glutathione redox system might contribute in some extent to this tolerance, as effective antioxidant defence against oxidative stress.

scholarly journals Short-term effects of T-2 toxin or deoxynivalenol on glutathione status and expression of its regulatory genes in chicken

2018 ◽  
Vol 66 (1) ◽  
pp. 28-39 ◽  
Author(s):  
Mangesh Nakade ◽  
Csilla Pelyhe ◽  
Benjámin Kövesi ◽  
Krisztián Balogh ◽  
Balázs Kovács ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Redox System ◽  
Glutathione Synthetase ◽  
Glutathione Peroxidase Activity ◽  
Short Term ◽  
Glutathione Redox System ◽  
Glutathione Status ◽  
Reductase Gene ◽  
Free Radical Formation ◽  
Glutathione Redox

Short-term (48-hour) effects of 3.74/1.26 mg kg−1 T-2/HT-2 toxin or 16.12 mg kg−1 DON in feed were investigated in the liver of three-week-old cockerels (body weight: 749.60 ± 90.98 g). Markers of lipid peroxidation showed no significant changes. At hour 24, glutathione content in the T-2/HT-2 toxin group was significantly higher than in the control. Glutathione peroxidase activity was significantly higher than the control at hour 24 in the T-2/H-2 toxin group and at hour 48 in the DON group. In the DON group, expression of the glutathione peroxidase 4 gene (GPX4) was significantly lower than in the control at hours 12 and 14, and higher at hour 48. Expression of the glutathione reductase gene (GSR) was significantly lower than in the control at hour 12 in the T-2/HT-2 toxin group, and at hours 12, 24 and 48 in the DON group. However, at hour 36 higher GSR expression was measured in the DON group. Due to the effect of both trichothecenes, expression of the glutathione synthetase gene (GSS) was significantly lower than in the control at hours 24 and 48. In conclusion, T-2/HT-2 toxin and DON had a moderate short-term effect on free radical formation. T-2/HT-2 toxin induced more pronounced activation of the glutathione redox system than did DON.

scholarly journals Experience of measuring glutathione peroxidase activity in surgically induced endometrial-like lesions in rats

2021 ◽  
Vol 70 (2) ◽  
pp. 55-61
Author(s):  
Aleksey V. Razygraev ◽  
Elena V. Baziyan ◽  
Lyudmila S. Polyanskikh ◽  
Mariya A. Petrosyan
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Rat Model ◽  
Peroxidase Activity ◽  
Specific Activity ◽  
Reduced Glutathione ◽  
Glutathione Peroxidase Activity ◽  
Nitrobenzoic Acid ◽  
Surgically Induced

BACKGROUND: Endometriosis is known to be linked with altered activities of antioxidant enzymes and with their gene polymorphisms. Progestins are known to induce glutathione peroxidase activity in the endometrium and promote reduction of endometrial lesions. It could be useful to estimate the correlation between the activity of glutathione peroxidase within endometrial lesions and their degree of reduction. AIM: The present study was aimed at estimating glutathione peroxidase activity in surgically induced endometrial-like lesions of different degree of reduction in rat model of endometriosis. MATERIALS AND METHODS: The method for determining glutathione peroxidase activity using hydrogen peroxide as a substrate and 5,5-dithiobis(2-nitrobenzoic acid) for estimation of residual reduced glutathione was applied for quantitative analysis of the enzyme activity in endometriotic foci, surgically induced in female Wistar rats. An assay of glutathione peroxidase activity in tissue homogenates was performed at 37C in a reaction medium containing Tris-HCl buffer supplemented with tetrasodium ethylenediaminetetraacetate and sodium azide (pH 8.5) in the presence of 0.55 mM reduced glutathione and 0.192 mM hydrogen peroxide. Before adding trichloroacetic acid, 40-second incubation was used. The correlation between the specific activity of the enzyme and protein amount in endometriotic foci was estimated. RESULTS: In a rat model of endometriosis, there was a high, well-determined glutathione peroxidase activity in endometriotic foci. For the same endometriotic tissue sample, the enzymatic activity was proportional to the amount of protein in the reaction mixture. The range of specific glutathione peroxidase activity was 2.436.45 micromoles of consumed glutathione per minute per milligram of protein (n = 7). In most reduced endometriotic foci (with the minimum amount of endometriotic tissue), the highest specific activity of glutathione peroxidase was found (the Spearmans rho of 0.93 with p = 0.0067). CONCLUSIONS: The method for determining glutathione peroxidase activity using hydrogen peroxide and 5,5-dithiobis(2-nitrobenzoic acid) is convenient for working with the endometriotic tissue in a rat model of endometriosis. We can accept, with p 0.01, that weight of endometriotic foci is negatively linked with specific glutathione peroxidase activity within their tissue. The results are analogous to the previously obtained data on catalase activity and suggest the involvement of both antioxidant enzymes in reduction of endometrial lesions.

Intracellular Pro-oxidant Activity of Melatonin Deprives U937 Cells of Reduced Glutathione without Affecting Glutathione Peroxidase Activity

2006 ◽  
Vol 1091 (1) ◽  
pp. 10-16 ◽  
Author(s):  
MARIA CRISTINA ALBERTINI ◽  
FLAVIA RADOGNA ◽  
AUGUSTO ACCORSI ◽  
FRANCESCO UGUCCIONI ◽  
LAURA PATERNOSTER ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Reduced Glutathione ◽  
U937 Cells ◽  
Glutathione Peroxidase Activity

scholarly journals Glutathione redox system, immune status, antioxidant enzymes and metabolism of purine nucleotides in hypothyroidism

2015 ◽  
Vol 61 (6) ◽  
pp. 737-741 ◽  
Author(s):  
S.O. Tapbergenov ◽  
B.S. Sovetov ◽  
R.B. Bekbosynova ◽  
S.M. Bolysbekova
Keyword(s):  
Antioxidant Enzymes ◽  
Immune Status ◽  
Reduced Glutathione ◽  
Redox System ◽  
Nucleotide Metabolism ◽  
Purine Nucleotides ◽  
Glutathione Redox System ◽  
Activity Of Enzymes ◽  
Purine Nucleotide Metabolism ◽  
Glutathione Redox

The immune status, components of the glutathione redox system, the activity of antioxidant enzymes and metabolism of purine nucleotides have been investigated in animals with experimental hypothyroidism. On day 8 after an increase in the number of leukocytes, lymphocytes, T-helpers and T-suppressors as well as increased number of B-lymphocytes was found in blood of thyroidectomized rats. This was accompanied by decreased activity of adenosine deaminase (AD), AMP-deaminase (AMPD), and 5'-nucleotidase (5'N) in blood, but the ratio of enzyme activity AD/AMPD increased. These changes in the activity of enzymes, involved in purine catabolism can beregarded as increased functional relationships between T and B lymphocytes in hypothyroidism. The functional changes of immune system cells were accompanied by increased activity of glutathione peroxidase (GPx), a decrease in the activity of superoxide dismutase (SOD), glutathione reductase (GR) and the ratio GH/GPx. Thyroidectomized rats had increased amounts of total, oxidized (GSSG) and reduced glutathione (GSH), but the ratio GSH/GSSG decerased as compared with control animals. In the liver, hypothyroidism resulted in activation of SOD, GPx, decreased activity of GR and decreased ratio GR/GPx. At the same time, the levels of total, oxidized, and reduced glutathione increased, but the ratio GSH/GSSG as well as activities of enzymes involved in purine nucleotide metabolism ratio (and their ratio 5'N/AD + AMPD) decreased. All these data suggest a functional relationship of the glutathione redox system not only with antioxidant enzymes, but also activity of enzymes involved purine nucleotide metabolism and immune status.

scholarly journals Effect of T-2 and HT-2 Toxin during the Growing Period on Body Weight, Lipid Peroxide and Glutathione Redox Status of Broiler Chickens

2010 ◽  
Vol 79 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Mária Weber ◽  
Krisztián Balogh ◽  
Judit Fodor ◽  
Márta Erdélyi ◽  
Zsolt Ancsin ◽  
...  
Keyword(s):  
Body Weight ◽  
Glutathione Peroxidase ◽  
Blood Cell ◽  
Blood Plasma ◽  
Red Blood Cell ◽  
Peroxidase Activity ◽  
Broiler Chickens ◽  
Reduced Glutathione ◽  
Liver Homogenate ◽  
Glutathione Peroxidase Activity

The effect of T-2 and HT-2 toxin using different doses in the starter (0-21 days: 1.04 mg T-2 toxin and 0.49 mg HT-2 toxin kg-1feed), and finisher diets (22-39 days: 0.12 mg T-2 toxin and 0.02 mg HT-2 toxin kg-1feed) was investigated in broiler chickens. Birds were divided into two groups fed with control and T-2 and HT-2 toxin contaminated diets. Pathological signs of toxicity were investigated on days 21 and 39 of the trial, individual liveweight was measured weekly. Five birds from each group were sacrificed on the 21st and 39th days of treatment, when blood plasma, red blood cell, liver and kidney samples were taken, in which malondialdehyde and reduced glutathione concentration and glutathione-peroxidase activity were determined. Pathological signs (lesions in the oral cavity and on the tongue, inflammation in the small intestine) were found in the group fed T-2 and HT-2 toxin contaminated feed on day 21 compared to control. Body weight was significantly lower as a result of feeding T-2 and HT-2 toxin contaminated diet. However, the contamination did not cause a significant increase of malondialdehyde content in the analysed tissues. Reduced glutathione content was significantly lower in the liver homogenate on day 39 than that of the control. Glutathione peroxidase activity also did not differ significantly in blood plasma, red blood cell haemolysates and kidney homogenates, while it was significantly higher in the liver homogenates of the mycotoxin-challenged birds. In conclusion, it can be stated that T-2 and HT-2 toxin exposure has long-term effects in broiler chickens.

scholarly journals Glutathione system in rat blood serum under the conditions of carrageenan-induced joint inflammation and chondroitin sulfate prophylactic injection

2021 ◽  
Vol 85 (2) ◽  
pp. 32-37
Author(s):  
K. Dvorshchenko ◽  
O. Korotkiy ◽  
D. Grebinyk ◽  
Ye. Torgalo
Keyword(s):  
Glutathione Peroxidase ◽  
Chondroitin Sulfate ◽  
Peroxidase Activity ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Joint Inflammation ◽  
Transferase Activity ◽  
Glutathione System ◽  
Nacl Solution ◽  
Glutathione Peroxidase Activity

The goal of this work was the research of chondroitin sulfate prophylactic action on the glutathione system state in rat blood serum during acute joint inflammation. The research was performed on white non-linear pubertal rat males weighting 160-240 grams in compliance to all general ethical principles of animal research. All animals were divided to four experimental groups. The first group – the control: the animals were injected with 0,1 ml of 0,9 % NaCl solution in a right hind leg. The second group – rats were subjected to daily intramuscular injections of 3 mg/kg of chondroitin sulfate in the theurapeutic dose during 28 days. The third group – the animals were subjected to daily doses of 0,1 ml of 0,9 % NaCl solution injected in right hind extremities and starting from 29th day the acute joint inflammation was modelled (the animals were subjected to 0,1 ml of 1% of сarrageenan intramuscular injection in right hind extremities. The fourth group – rats were receiving intramuscular injections of therapeutic dose of 3 mg/kg of chondroitin sulfate for 28 days, and after that the acute joint inflammation was modelled starting from 29th day. 40 animals in all were taking part in the experimental research. The glutathione peroxidase activity was assessed judging from the decrease in GSH amount in the reaction with Ellman reagent. The glutathione peroxidase activity was estimated by the decrease in probe optical density owing to NADPH oxidation. Glutathione transferase activity was estimated by the speed of the conjugate formation between GSH and 1-chloro-2,4-dinitrobemzene. The amount of reduced glutathione was estimated spectrophotometrically using orthophthalic aldehyde. It was established that during the carrageenan-induced inflammation the glutathione peroxidase, glutathione reductase and glutathione reductase activity were reduced, a well as the amount of reduced glutathione, whereas the glutathione transferase activity was increased in comparison to the control. It was shown that under the prophylactic injection of the chondroitin sulfate based preparate to animals with acute joint inflammation the aforementioned parameters were partially stabilized.

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