Preparation and In Vitro Characterization of Polycaprolactone and Demineralized Bone Matrix Scaffolds

2012 ◽  
Vol 1417 ◽  
Author(s):  
Titilayo Moloye ◽  
Christopher Batich
Keyword(s):  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Apatite Formation ◽  
Alizarin Red ◽  
Salt Leaching ◽  
Synthetic Materials ◽  
Calcium And Phosphorus ◽  
Demineralized Bone

ABSTRACTCylindrical porous polycaprolactone (PCL) scaffolds containing 25, 35, and 50 wt% demineralized bone matrix (DBM) were fabricated using a salt-leaching method for application in bone engineering. In the present work, PCL-DBM scaffolds were monitored for calcium and phosphorus deposition in both deionized (DI) water and simulated body fluid (SBF) for time periods of 5, 10, 15, and 20 days at 37°C under constant rotation. An in vitro assessment of the bioactivity of synthetic materials using SBF under physiological conditions can be used as a barometer of scaffold behavior in vivo. DBM, an osteoinductive material, was used to gauge if there was a correlation between the concentration of DBM within a scaffold and the apatite formation on its surface. Biochemical assays, alizarin red S staining, and scanning electron microscopy (SEM) with elemental analysis of calcium and phosphorus were consistent in that they confirmed that PCL scaffolds containing 35 wt% DBM in SBF at 14 days post-immersion showed signs of early apatite formation.

In vitro and in vivo evaluation of xenogeneic bone putty with the carrier of hydrogel derived from demineralized bone matrix

2018 ◽  
Vol 19 (4) ◽  
pp. 591-601 ◽  
Author(s):  
Naili Zhang ◽  
Lina Ma ◽  
Xiaowei Liu ◽  
Xiaorui Jiang ◽  
Zhenhai Yu ◽  
...  
Keyword(s):  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
In Vivo Evaluation ◽  
Demineralized Bone ◽  
Bone Putty

Rheological properties, biocompatibility and in vivo performance of new hydrogel-based bone fillers

2016 ◽  
Vol 4 (11) ◽  
pp. 1691-1703 ◽  
Author(s):  
Paolo Giannoni ◽  
Federico Villa ◽  
Cinzia Cordazzo ◽  
Luciano Zardi ◽  
Paolo Fattori ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Rheological Properties ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Demineralized Bone

Three different heterologous substitutes for bone regeneration, manufactured with equine-derived cortical powder, cancellous chips and demineralized bone matrix granules, were compared in vitro and in vivo.

In Vitro and In Vivo Evaluation of a Calcium Phosphate and Demineralized Bone Matrix Composite Biomaterial

2010 ◽  
Vol 5 ◽  
pp. 1-12 ◽  
Author(s):  
A. Rosenberg ◽  
Aliassghar Tofighi ◽  
N. Camacho ◽  
J. Chang
Keyword(s):  
Calcium Phosphate ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Negative Control ◽  
Water Soluble ◽  
Dense Area ◽  
Viscosity Modifier ◽  
Demineralized Bone

A new class of osteoconductive and osteoinductive combination biomaterials composed of calcium phosphate cement (CPC), demineralized bone matrix (DBM) and a water-soluble viscosity modifier were prepared and characterized in-vitro and in-vivo. In previous studies, a range of combinations formulations were tested in order to compare their performance characteristic. In-vitro characterization results show that the mechanical strength is decreased when the amount of DBM increases. However, DBM does not affect the CPC’s ability to set hard and convert to nanocrystalline apatitic calcium phosphate, which shares the chemical structure of natural bone as seen in x-ray diffraction. It is known that the DBM alone is osteoinductive. In-vivo osteoinductivity testing of the formulations in an intramuscular, athymic rat model demonstrated that the combination material is also osteoinductive. Two formulations were chosen for in-vivo efficacy testing based on the results of in-vitro and in-vivo characterization. These formulations were studied using rabbit critical-sized femoral core defect model. The formulations were composed of DBM with particle sizes of 250 to 710 μm, carboxymethyl-cellulose (CMC) as the viscosity modifier and weight percent compositions of 50% DBM/ 45% CPC/ 5% CMC and 60% DBM/ 30% CPC/ 10% CMC. Bone integration and healing was graded at 6, 12, and 24 weeks. The two formulations were compared to the gold standard autograft at 12 weeks and to an empty defect as the negative control at 24 weeks. Based on micro-computed topography (μCT), both formulations allowed for continuity of bone throughout the defect region at all time points. No differences in dense area fraction were seen between two formulations at 6 weeks (p = 0.8661). There was no significant statistical difference between the two formulations and autograft at 12 weeks (p = 0.2467). At 24 weeks, both formulations had significantly higher dense area fractions than empty controls (p = 0.0001). Histologically, the biology of the treatment areas appeared to have returned to normal by 24 weeks with CPC appearing to be the principal osteogenic inducer. In conclusion, these combinations of CPC and DBM offers significant advantages (handling, mechanical properties and osteoinductivity) over current DBM products and can be an effective alternative to autograft in healing of bone defects.

scholarly journals Implant Composed of Demineralized Bone and Mesenchymal Stem Cells Genetically Modified with AdBMP2/AdBMP7 for the Regeneration of Bone Fractures inOvis aries

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Adelina A. Hernandez-Hurtado ◽  
Gissela Borrego-Soto ◽  
Ivan A. Marino-Martinez ◽  
Jorge Lara-Arias ◽  
Viktor J. Romero-Diaz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Fractures ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Adenoviral Vectors ◽  
Bone Tissue Regeneration ◽  
Demineralized Bone

Adipose-derived mesenchymal stem cells (ADMSCs) are inducible to an osteogenic phenotype by the bone morphogenetic proteins (BMPs). This facilitates the generation of implants for bone tissue regeneration. This study evaluated thein vitroosteogenic differentiation of ADMSCs transduced individually and in combination with adenoviral vectors expressing BMP2 and BMP7. Moreover, the effectiveness of the implant containing ADMSCs transduced with the adenoviral vectors AdBMP2/AdBMP7 and embedded in demineralized bone matrix (DBM) was tested in a model of tibial fracture in sheep. This graft was compared to ewes implanted with untransduced ADMSCs embedded in the same matrix and with injured but untreated animals.In vivoresults showed accelerated osteogenesis in the group treated with the AdBMP2/AdBMP7 transduced ADMSC graft, which also showed improved restoration of the normal bone morphology.

Experimental Study of Partially Demineralized Bone Matrix as Bone Tissue Engineering Scaffold

2005 ◽  
Vol 288-289 ◽  
pp. 63-66 ◽  
Author(s):  
Lei Cui ◽  
Dong Li ◽  
Xiang Dong Liu ◽  
Fanfan Chen ◽  
Wei Liu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Calcium Content ◽  
Post Surgery ◽  
Demineralized Bone

Objective The purpose of this study is to explore the growth, differentiation and osteogeneration of bone marrow stromal cells (BMSCs) on partially demineralized bone matrix (pDBM) and to generate bone tissue by tissue engineering approach in vivo. Methods Demineralized bone was processed from femur head of Shanghai white swine. Calcium content, porosity and pore size was measured respectively. In vitro osteogenic differentiated human BMSCs of passage 3 were seeded in pDBM. Adhesive rate of cells to pDBM was calculated 24hours after seeding. Distribution, growth and proliferation of BMSCs on pDBM were observed with fluorescent DiI labeling. Matrix disposition was analyzed with SEM observation. Cell-material complex was implanted subcutaneously in nude mice. The implants were harvested at 8, 12 weeks post surgery and samples were observed by H&E staining. Results BMSCs adhered well on the material and the distribution of cells was uniform. The adhesive rate is 99.1%±1%. New bone formation was observed in implant of 8, 12 weeks respectively. The newly formed bone was generated on the surface of the residual material and a layer of cells with typical characteristic of osteoblast was observed to adhere on the surface of the new bone. Conclusion With good biocompatibility to hBMSCs, pDBM could serve as ideal scaffold for bone tissue engineering both in vitro and in vivo.

The Osteogenetic Efficacy of Goat Bone Marrow-Enriched Self-Assembly Peptide/Demineralized Bone Matrix In Vitro and In Vivo

2015 ◽  
Vol 21 (7-8) ◽  
pp. 1398-1408 ◽  
Author(s):  
Zhiqiang Li ◽  
Tianyong Hou ◽  
Moyuan Deng ◽  
Fei Luo ◽  
Xuehui Wu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Self Assembly ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Demineralized Bone

In Vivo Bone Tissue Formation Induced by Caclium Phosphate Paste Composite with Demineralized Bone Matrix

2007 ◽  
Vol 330-332 ◽  
pp. 1091-1094
Author(s):  
H. Kim ◽  
M. Park ◽  
Su Young Lee ◽  
Kang Yong Lee ◽  
Hyun Min Kim ◽  
...  
Keyword(s):  
Animal Model ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Marker Gene ◽  
Tissue Formation ◽  
Bone Tissue Formation ◽  
Demineralized Bone

Demineralized bone matrix (DBM)-calcium phosphate cement (CPC) composites were subjected to cellular test of osteogenic potentials and implantation in animal model. The expression of osteogenic marker gene from mouse preosteoblast cell line MC3T3-E1 adhered to the DBM-CPC composite was much higher than plain CPC. In addition, the DBM-CPC composite implanted nude mice revealed osteoinduction between the implanted composite and adjacent tissues, whereas the plain CPC induced osteoconduction.

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