scholarly journals ISOLASI DAN IDENTIFIKASI KAPANG PADA PINDANG BANDENG (Chanos chanos) PRESTO

2019 ◽  
Vol 2 (1) ◽  
pp. 15
Author(s):  
Resmi Rumenta Siregar
Keyword(s):  
Yeast Extract ◽  
Rose Bengal ◽  
Fusarium Solani ◽  
Penicillium Citrinum ◽  
Malt Extract Agar ◽  
Malt Extract ◽  
Chanos Chanos ◽  
Extract Agar ◽  
Fusarium Sp ◽  
Yeast Extract Agar

Ikan pindang adalah salah satu olahan yang sangat disukai oleh masyarakat Indonesia. Hal ini dapat dilihat dari produksi ikan pindang yang setiap tahunnya mengalami peningkatan. Sebagai contoh di Kabupaten Bogor, produksi ikan pindang pada tahun 2013 sebesar 3.643,56 ton, meningkat menjadi 10.334,44ton pada tahun 2015. Ikan pindang disisi lain, sangat mudah mengalami kemunduran mutu disebabkan masih tingginya kadar air, pengemasan yang tidak memenuhi standar serta proses pengolahan yang pada umumnya kurang menerapkan prinsip sanitasi yang baik. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kapang yang tumbuh pada bandeng presto. Sampel Bandeng presto diambil dari CV. Cindy Group. Kapang diisolasi dengan metode pengenceran bertingkat menggunakan media DRBC (Dichloran Rose Bengal Chloramphenicol Agar), kemudian diidentifikasi secara morfologi menggunakan media Malt Extract Agar dan Czapek’s Yeast Extract Agar. Nilai Aktivitas air (aw) bandeng presto memiliki kisaran rata-rata 0,96 – 0,97. Secara makroskopis terlihat adanya pertubuhan kapang pada permukaan ikan bandeng presto setelah penyimpanan selama 3 hari pada suhu ruang (20-250C). Sebanyak 5 isolat kapang diisolasi dari ikan pindang sampel bandeng presto. Hasil identifikasi secara mikroskopis diketahui bahwa kapang yang tumbuh ada ikan pindang tersebut adalah spesies Penicillium citrinum, Eurotium chevalieri, Fusarium solani, Fusarium sp, dan Cladosporium sp. Kadar aw ikan pindang resto yang masih tinggi (0,96-0,97) menyebabkan ikan pindang mengalami pembusukan yang diakibatkan oleh bakteri.  

scholarly journals Pertumbuhan Fusarium verticillioides, Aspergillus flavus, dan Eurotium chevalieri pada Berbagai Media

2017 ◽  
Vol 2 (03) ◽  
pp. 39-41
Author(s):  
Gergonius Fallo
Keyword(s):  
Aspergillus Flavus ◽  
Yeast Extract ◽  
Fusarium Verticillioides ◽  
Malt Extract Agar ◽  
Malt Extract ◽  
Extract Agar ◽  
X 24 ◽  
Yeast Extract Agar

Fusarium verticillioides, Aspergillus flavus, dan Eurotium chevalieri merupakan cendawan perusak bahan pangan yang ditemukan pada saat bahan pangan belum dipanen atau setelah bahan pangan dipanen. Pertumbuhan ketiga cendawan ini sangat dipengaruhi oleh nutrisi yang terkandung pada media tumbuh. Penelitian ini bertujuan untuk membandingkan pertumbuhan Fusarium verticillioides, Aspergillus flavus, dan Eurotium chevalieri pada beberapa media untuk isolasi dan identifikasi. Ketiga cendawan tersebut ditumbuhkan pada media yang berbeda yaitu Chloramphenicol Peptone Agar (DCPA), Dichloran 18% Glycerol Agar (DG18), Czapek Yeast Extract Agar (CYA), Czapek Yeast Extract Agar 20% Sucrose (CY20S), Malt Extract Agar (MEA), dan 25% Glycerol Nitrate Agar (G25N) dan diinkubasi pada suhu + 280C. Pengamatan koloni dilakukan setiap 2 x 24 jam, 4 x 24 jam, dan 6 x 24 jam selanjutnya diameter isolat diukur. Hasil isolasi pertumbuhan dan panjang diameter koloni dari A. Flavus (61 mm) dan E. Chevalieri (45mm) diketahui dapat tumbuh baik pada media DG18, sedangkan F. verticillioides (46 mm) tumbuh baik di media DCPA. Sementara pada media identifikasi A. Flavus (80 mm) dapat tumbuh baik pada media CY20S, sedangkan E. Chevalieri (40 mm) dapat tumbuh baik pada media G25N dan CY20S. Koloni F. verticillioides (62 mm) dapat tumbuh baik di media CYA dan CY20S. ©2017 dipublikasikan oleh Savana Cendana.

Comparison of Antibiotic-Containing Media and Media Supplemented with Dichloran and Rose Bengal for Isolation and Enumeration of Fungi in Spices

1986 ◽  
Vol 49 (10) ◽  
pp. 815-817 ◽  
Author(s):  
JOANNA H. ROGERS ◽  
PHILIP S. GUARINO
Keyword(s):  
Yeast Extract ◽  
Rose Bengal ◽  
Colony Morphology ◽  
Plate Count ◽  
Extract Agar ◽  
Plate Count Agar ◽  
Agar Media ◽  
Yeast Extract Agar

Yeast and mold counts of various spice products were determined using Dichloran Rose Bengal agar, Phytone Yeast Extract agar with added Dichloran and Rose Bengal, and Antibiotic Plate Count agar. Media containing the added Dichloran and Rose Bengal proved superior to media without Dichloran and Rose Bengal in controlling mold overgrowth, and promoting distinct colony morphology. Results were obtained 2 d earlier using Phytone Yeast Extract agar with added Dichloran and Rose Bengal.

Freshwater hyphomycete communities in a canal. 1. Endophytic hyphomycetes of submerged roots of trees sheltering a canal bank

1995 ◽  
Vol 73 (4) ◽  
pp. 538-543 ◽  
Author(s):  
S. H. Iqbal ◽  
Firdaus-e- Bareen ◽  
Noureen Yousaf
Keyword(s):  
Key Words ◽  
Tree Species ◽  
Mangifera Indica ◽  
Malt Extract Agar ◽  
Malt Extract ◽  
Brick Wall ◽  
Canal Water ◽  
Extract Agar ◽  
Fusarium Sp ◽  
Salix Babylonica

Seventeen species of freshwater hyphomycetes were found as endophytes of roots of Mangifera indica, Populus hybrida, and Salix babylonica extending into the water from crevices in the brick wall of a canal in Lahore, Pakistan. More species were recovered by aerating than by plating bark and xylem segments on malt extract agar. Endophytic species of bark outnumbered those of xylem detected by aeration and plating methods. Anguillospora longissima, Articulospora proliferata, Bacillispora aquatica, Flagellospora curvula, Flagellospora fusarioides, Flagellospora penicillioides, Fusarium sp., and Tetracladium marchalianum were produced in abundance on aerated root segments. Fusarium sp. dominated other endophytic species of three tree species. Fifteen, 14, and 12 species were recovered from Salix babylonica, Mangifera indica, and Populus hybrida, respectively. Campylospora chaetocladia was confined on the bark and Heliscus lugdunensis on the bark as well as xylem segments of Salix babylonica. Clavariopsis azlanii and Tricellula aquatica were recovered from roots of Mangifera indica. Key words: endophytic freshwater hyphomycetes, submerged roots, canal water.

scholarly journals عزل وتعريف واختبار كفاءة بعض الفطريات في تحلل الهيدروكربون من التربة الملوثة بالنفط

2020 ◽  
Vol 3 (1) ◽  
pp. 78-90
Author(s):  
سعاد محمد خليفة أبوالغيث ◽  
أحلام القمودي محمد زعيط
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Aspergillus Nidulans ◽  
Aspergillus Flavus ◽  
Malt Extract Agar ◽  
Malt Extract ◽  
Extract Agar

استهدفت هذه الدراسة عزل بعض أنواع الفطريات من التربة الملوثة بالهيدروكربون بمصفاة الزاوية لتكرير النفط، حيث تم عزل وتعريف بعض الفطريات مثل Rhizopus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus, Aspergillus nidulans وأوضحت نتائج هذه الدراسة أن تواجد وتنوع فطر Aspergillus قد تفوق معنويا مقارنة بتواجد وتنوع فطرRhizopus. كما تم في هذه الدراسة اختبار قدرة وكفاءة الفطريات المعزولة على النمو واستغلال المركبات الهيدروكربونية المتمثلة في زيت الحمادة وزيت الشرارة بتركيز 1% و3%، حيث أوضحت النتائج بأن جنس Rhizopus سجل أعلى معدل للنمو على الوسط الغذائي Malt Extract Agar، وسجل كلا من فطر A. fumigatus وفطر A. flavus معدّل النمو القطري أعلى معنويا من النمو القطري لفطرA. niger  وفطر A. nidulans. هذه المعدّلات العالية تدل على إمكانية استخدام الفطريات المعزولة في المعالجة البيولوجية للتربة الملوّثة بالنفط.

Comparison of agar-based methods for the isolation and enumeration of heterotrophic bacteria with the new Multidose IDEXX(tm) SimPlate method

2004 ◽  
Vol 50 (1) ◽  
pp. 277-280
Author(s):  
M. Vulindlu ◽  
A. Charlett ◽  
S. Surman ◽  
J.V. Lee
Keyword(s):  
Yeast Extract ◽  
Heterotrophic Bacteria ◽  
Treatment Plant ◽  
Plant Performance ◽  
Extract Agar ◽  
Quality Of Water ◽  
Heterotrophic Microorganisms ◽  
External Sources ◽  
Conventional Methods ◽  
Yeast Extract Agar

Pour and spread plates are the conventional methods of choice for the isolation and enumeration of heterotrophic microorganisms in treated water supplies. The tests are performed at 22°C and 37°C for 72 h and 48 h respectively. Counts at 22°C are associated with pollution of water systems from external sources, while counts at 37°C are used as an indication of treatment plant performance and the deterioration of the general quality of water. Conventional methods using Yeast Extract Agar for a pour plate and R2A agar for a spread plate were compared with the multidose IDEXXTM SimPlate method for the isolation and enumeration of heterotrophic bacteria in water. SimPlate gave a significantly higher count on average than the conventional methods. The R2A method showed the next highest count, being significantly higher than Yeast Extract Agar. In addition, unlike the pour and spread plate methods, SimPlate was easier to use, reduced labour, and the test results were far easier to read.

Nannocystis exedens: A Potential Biocompetitive Agent against Aspergillus flavus and Aspergillus parasiticus

2001 ◽  
Vol 64 (7) ◽  
pp. 1030-1034 ◽  
Author(s):  
WILLIE J. TAYLOR ◽  
FRANCES A. DRAUGHON
Keyword(s):  
Aspergillus Flavus ◽  
Yeast Extract ◽  
Biocontrol Agent ◽  
Aspergillus Parasiticus ◽  
Extract Agar ◽  
Potential Biocontrol Agent ◽  
Aflatoxigenic Fungi ◽  
Close Proximity ◽  
Zones Of Inhibition ◽  
Yeast Extract Agar

This study examined the potential for controlling toxigenic Aspergillus flavus and Aspergillus parasiticus by biological means using a myxobacterium commonly found in soil. The ability of Nannocystis exedens to antagonize A. flavus ATCC 16875, A. flavus ATCC 26946, and A. parasiticus NRRL 3145 was discovered. Cultures of aflatoxigenic fungi were grown on 0.3% Trypticase peptone yeast extract agar for 14 days at 28°C. When N. exedens was grown in close proximity with an aflatoxigenic mold, zones of inhibition (10 to 20 mm) developed between the bacterium and mold colony. A flattening of the mold colony on the sides nearest N. exedens and general stunting of growth of the mold colony were also observed. When N. exedens was added to the center of the cross-streak of a mold colony, lysis of the colony by the bacterium was observed after 24 h. Microscopic observations revealed that N. exedens grew on spores, germinating spores, hyphae, and sclerotia of the molds. These results indicate that N. exedens may be a potential biocontrol agent against A. flavus and A. parasiticus.

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