scholarly journals VAKSINASI IKAN TILAPIA (Oreochromis niloticus) MENGGUNAKAN VAKSIN MONOVALEN DAN BIVALEN UNTUK PENCEGAHAN PENYAKIT MOTILE AEROMONAS SEPTICEMIA DAN STREPTOCOCCOSIS

2013 ◽  
Vol 8 (2) ◽  
pp. 230 ◽  
Author(s):  
Desy Sugiani ◽  
Sukenda Sukenda ◽  
Enang Harris ◽  
Angela Mariana Lusiastuti
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Percent Survival ◽  
Neutral Buffer Formalin

Peningkatan respon antibodi pascavaksinasi dengan antigen tunggal dan campuran dari bakterin Aeromonas hydrophila and Streptococcus agalactiae diharapkan dapat meningkatkan daya tahan ikan tilapia (Oreochromis niloticus) terhadap penyakit Motile Aeromonas Septicemia/MAS dan Streptococcosis. Sediaan vaksin disiapkan dengan metode pembuatan dan formula yang berbeda, proses inaktifasi dilakukan dengan menambahkan 3% Neutral Buffer Formalin (NBF 10%) pada biakan bakteri dalam media tumbuh BHI dan TSB. Vaksinasi diberikan melalui injeksi intraperitoneal dengan sediaan vaksin monovalen A. hydrophila, monovalen S. agalactiae, dan bivalen A. hydrophila + S. agalactiae (Sel utuh, produk ektraselular/ECP, crude supernatan, campuran sel utuh + ECP, dan broth). Uji tantang dilakukan menggunakan dosis LD50 infeksi tunggal maupun ko-infeksi dari bakteri A. hydrophila dan S. agalactiae. Efektivitas dan keampuhan vaksin tersebut dihitung berdasarkan nilai RPS (Relative Percent Survival) dan hasil respon hematologi. Titer antibodi dapat terdeteksi setelah satu minggu pemeliharaan pasca vaksinasi. Nilai titer antar perlakuan vaksin bivalen berbeda nyata (P<0.05) dengan vaksin monovalen dan kontrol. Nilai RPS vaksin bivalen (campuran sel utuh + ECP) mencapai 100 untuk uji tantang dengan A. hydrophila dan 56,7 pada uji tantang ko-infeksi. Vaksin monovalen A. hydrophila maupun S. agalactiae hanya mampu memproteksi terhadap bakteri homolog, tidak terjadi proteksi silang di antara keduanya.

scholarly journals THE EFFECT OF GIVING ONION (Allium Sativum) EXTRACT TO TREAT THE SEED OF TILAPIA (Oreochromis Niloticus) BACTERIAL INFECTED Aeromonas hydrophila

2021 ◽  
Vol 1 (2) ◽  
pp. 80-87
Author(s):  
Ani Lestari ◽  
Nurliah ◽  
Baiq Hilda Astriana
Keyword(s):  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Aquatic Environment ◽  
Allium Sativum ◽  
Bacterial Resistance ◽  
Survival Rates ◽  
Garlic Extract ◽  
Percent Survival ◽  
Randomized Design ◽  
Prevention And Treatment

MAS disease (Motile Aeromonas Septicemia) is a disease caused by A. hydrophila bacteria that attack tilapia and other freshwater fish species. The use of antibotics and chemicals in the continuous prevention and treatment of this disease can damage the aquatic environment and can cause bacterial resistance to antibotics, so antibotic use becomes ineffective. The study used a Complete RandomIzed Design (RAL) with an experimental method consisting of 5 treatments and 3 repeats. Treatment in this study is (P1) without soaking garlic extract and without infected bacteria Aeromonas hydrophila, (P2) without soaking garlic extract and infected bacteria Aeromonas hydrophila, (P3) soaking garlic extract with a concentration of 1% and disinfection of bacteria Aeromonas hydrophila, (P4) immersion of garlic extract with a concentration of 1.5% and infected bacteria Aeromonas hydrophila, (P5) soaked garlic extract with a concentration of 2% and infected with Aeromonas hydrophila bacteria. The results showed that the SR value of tilapia fish in each treatment had values ranging from 0.0% to 66.7%. Relative percent survival (RPS) tilapia seeds in each treatment have values ranging from 0.0% to 66.7%. The conclusion of this study is that giving garlic extract with different concentrations has a real different influence on survival rates and relative percent survival (RPS).

PROFIL PROTEIN VAKSIN Aeromonas hydrophila DAN Streptococcus agalactiae HASIL INAKTIVASI DENGAN FORMALIN: DIUJI MENGGUNAKAN Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis

2014 ◽  
Vol 9 (3) ◽  
pp. 449
Author(s):  
Desy Sugiani ◽  
Angela Mariana Lusiastuti ◽  
Sukenda Sukenda ◽  
Enang Harris
Keyword(s):  
Streptococcus Agalactiae ◽  
Sodium Dodecyl Sulphate ◽  
Total Protein ◽  
Aeromonas Hydrophila ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Sds Page ◽  
Neutral Buffer Formalin

Vaksin bakterin dalam bentuk protein merupakan salah satu tipe vaksin yang telah banyak dikembangkan. Protein digunakan sebagai vaksin biasanya dibuat dengan teknik inaktivasi formalin-killed. Vaksin ini biasanya lebih mudah dibuat, lebih murah, lebih stabil, dan mampu disimpan dalam waktu lama. Akan tetapi masih sedikit informasi mengenai efek perlakuan tersebut terhadap profil protein. Pada penelitian ini, untuk mengevaluasi profil protein, dilakukan inaktivasi sediaan vaksin dari isolat bakteri Aeromonas hydrophila AHL0905-2 dan Streptococcus agalactiae N14G dengan menambahkan 0,5% formalin dan 3% neutral buffer formalin (NBF) ke dalam biakan plasebo bakterin dan diinkubasi selama 24 jam. Kualitas produk vaksin ditentukan berdasarkan uji karakterisasi protein menggunakan metode Bradford dan SDS-PAGE. Hasil uji menunjukkan bahwa sediaan vaksin A. hydrophila dan S. agalactiae yang diinaktivasi dengan 3% NBF memiliki profil protein lebih variatif dibandingkan dengan sediaan vaksin yang diinaktivasi dengan 0,5% formalin. Akan tetapi, inaktivasi vaksin A. hydrophila dan S. agalactiae dengan 3% NBF menghasilkan berat total protein yang lebih rendah jika dibandingkan dengan dengan sediaan vaksin yang diinaktivasi dengan 0,5% formalin.

scholarly journals Enterococcus, Myroides Y Exiguobacterium: GÉNEROS BACTERIANOS CON POTENCIAL PROBIÓTICO PARA EL CULTIVO DE TILAPIA NILÓTICA (Oreochromis niloticus)

2017 ◽  
Vol 22 (3) ◽  
pp. 331-339 ◽  
Author(s):  
Luisa Marcela Villamil Díaz ◽  
Daniel Esguerra Rodríguez
Keyword(s):  
16S Rrna ◽  
Enterococcus Faecalis ◽  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Edwardsiella Tarda ◽  
Tilapia Nilotica ◽  
Myroides Odoratimimus

Se aislaron 120 morfotipos bacterianos de intestino de tilapia y se seleccionaron según su actividad antibacteriana contra patógenos como Aeromonas hydrophila, Streptococcus agalactiae y Edwardsiella tarda, su capacidad de adherencia a mucus intestinal y cinética de crecimiento. Las bacterias seleccionadas se identificaron mediante secuenciación de 16S rRNA y se identificaron como Exigobacterium sp. I9, Enterococcus faecalis I15 y Myroides odoratimimus I19. Además, se evaluó su efecto in vivo sobre el crecimiento de los peces, mediante su adición al alimento de juveniles de Oreochromis niloticus (106 UFC / g, por 15 días). Se determinó la supervivencia luego de un desafío experimental con Edwardsiella tarda por inyección intraperitoneal (100 µL 105 UFC / mL). Las tres bacterias seleccionadas incrementaron la tasa de crecimiento específica, redujeron la mortalidad de los peces durante el desafío experimental con E. tarda y no causaron mortalidad durante la adición en el alimento. Los efectos positivos in vivo se relacionan posiblemente con actividad in vitro; sin embargo, por motivos de bioseguridad se recomienda efectuar estudios posteriores a Exigobacterium sp. I9y E. faecalis I15 dado que se han reportado miembros de este género como causantes de mortalidad en peces, mientras que en el caso de M. odoratimimus I19, es necesario efectuar futuros estudios para verificar su actividad positiva a mayor escala productiva.

scholarly journals PENGARUH KO-INFEKSI BAKTERI Streptococcus agalactiae DENGAN Aeromonas hydrophila TERHADAP GAMBARAN HEMATOLOGI DAN HISTOPATOLOGI IKAN TILAPIA ( Oreochromis niloticus )

2012 ◽  
Vol 7 (1) ◽  
pp. 85 ◽  
Author(s):  
Desy Sugiani ◽  
Sukenda Sukenda ◽  
Enang Harris ◽  
Angela Mariana Lusiastuti
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus

Karakteristik hasil ko-infeksi buatan dari penyakit Streptococcosis dan MAS (Motile Aeromonas Septicemia) dapat dilihat dengan menggunakan parameter gambaran hematologi dan histopatologi. Ikan tilapia (Oreochromis niloticus) ukuran 15 g diinfeksi secara intra peritoneal dengan bakteri Streptococcus agalactiae dan Aeromonas hydrophila menggunakan dosis LD50. Perubahan pertahanan non spesifik ikan terhadap infeksi patogen dilihat dengan mengamati level hematokrit, neutrofil, limfosit, monosit, dan indeks fagositik darah ikan tilapia yang diambil dari arteri caudalis pada hari ke-3, 6, 9, 12, dan 15 setelah infeksi. Hasil analisis perubahan level hematokrit dan limfosit lebih tinggi dibandingkan dengan kontrol, level neutrofil lebih rendah dibandingkan dengan kontrol, dan level monosit dan indeks fagositik fluktuatif selama masa perlakuan memperlihatkan adanya homeostasi gambaran darah ikan terhadap serangan infeksi antigen. Hasil histopatologi organ ginjal, otak, hati, dan limfa memperlihatkan dua pola karakter luka. Pola pertama, luka yang focal sampai terlihat adanya inflamasi dan perdarahan. Pola kedua, luka yang multifocal, luka parah (acute), nekrotik, dan luka inflamasi yang mengakibatkan deformasi sel-sel organ.

scholarly journals THE EFFECT OF ONION (Allium sativum) EXTRACT TO TREAT TILAPIA FRY (Oreochromis niloticus) WITH BACTERIAL INFECTED Aeromonas hydrophila

2021 ◽  
Vol 1 (1) ◽  
pp. 29-39
Author(s):  
Ani Lestari ◽  
Nurliah ◽  
Baiq Hilda Astriana
Keyword(s):  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Allium Sativum ◽  
Clinical Symptoms ◽  
Survival Rates ◽  
Garlic Extract ◽  
Study Program ◽  
Percent Survival ◽  
Randomized Design ◽  
Completely Randomized Design

Research on the effect of garlic extract (allium sativum) to treat tilapia (oreochromis niloticus) seeds which are infected with aeromonas hydrophila bacteria. This research was conducted for 14 days from 18 December to 31 December 2019 which took place at the Wet Laboratory of the Aquaculture Study Program, Mataram University, West Nusa Tenggara (NTB). This study used a completely randomized design (CRD) with an experimental method consisting of 5 treatments and 3 replications. The container used in this study was a 15 liter jar with a volume of 10 liters. The containers used are 15 units, where in treatment one (P1) using without soaking garlic extract and without being infected with Aeromonas hydrophila bacteria, treatment two (P2) without soaking garlic extract and being infected with Aeromonas hydrophila bacteria, (P3) using soaking onion extract white with a concentration of 1% and infected with Aeromonas hydrophila bacteria, (P4) using garlic extract immersion with a concentration of 1.5% and infected with Aeromonas hydrophila bacteria, (P5) using garlic extract immersion with a concentration of 2% and infected with Aeromonas hydrophila bacteria. The parameters observed during the study included survival rate (SR), relative percent survival (RPS), observation of clinical symptoms, and water quality. The results showed that giving garlic extract with different concentrations had a significantly different effect on survival rates and relative percent survival (RPS).

scholarly journals Pre-treatment of Nile tilapia (Oreochromis niloticus) with ozone nanobubbles improve efficacy of heat-killed Streptococcus agalactiae immersion vaccine

2021 ◽  
Author(s):  
Linh Vu Nguyen ◽  
Dien Thanh Le ◽  
Pattiya Sangpo ◽  
Saengchan Senapin ◽  
Anat Thapinta ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Bacterial Antigen ◽  
Fish Pathogens ◽  
Effective Response ◽  
Expression Levels ◽  
Percent Survival ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Pre Treatment

Nanobubble technology has shown appealing technical benefits and potential applications in aquaculture. We recently found that treatment with ozone nanobubbles (NB-O3) activated expression of several immune-related genes leading to effective response to subsequent exposure to fish pathogens. In this study, we investigated whether pre-treatment of Nile tilapia (Oreochromis niloticus) with NB-O3 can enhance specific immune responses and improve efficacy of immersion vaccination against Streptococcus agalactiae. Spleen and head kidney of fish in the vaccinated groups showed a substantial upregulation in expression levels of three immunoglobulin classes (IgM, IgD, and IgT) compared with the unvaccinated control groups. At day 21 post-immunization, the relative expression was greatest (approx. 3.2 to 4.1 folds). Both systemic and mucosal IgM antibodies were elicited in vaccinated groups. As the result, the cumulative survival rate of the vaccinated groups was found to be higher than that of the unvaccinated groups, with a relative percent survival (RPS) ranging from 52.9-70.5%. However, fish in the vaccinated groups that received pre-treatment with NB-O3, bacterial antigen uptakes, expression levels of IgM, IgD, and IgT, as well as the specific-IgM antibody levels and percent survival, were all slightly or significantly higher than that of the vaccinated group without pre-treatment with NB-O3. Taken together, our findings suggest that utilizing pre-treatment with NB-O3 may improve the immune response and efficacy of immersion vaccination in Nile tilapia.

scholarly journals Specific Immune Response Kinetics and Mortality Patterns of Tilapia Oreochromis niloticus on Post-Cocktail Vaccination Period against the Infection of Aeromonas hydrophila and Streptococcus agalactiae

Omni-Akuatika ◽  
2017 ◽  
Vol 13 (2) ◽  
Author(s):  
Sukenda Sukenda ◽  
Tuti Sumiati ◽  
Sri Nuryati ◽  
Angela Mariana Lusiastuti ◽  
Dendi Hidayatullah
Keyword(s):  
Immune Response ◽  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Specific Immune Response ◽  
Single Infection ◽  
Control Group ◽  
Antibody Levels ◽  
Kinetics Of

ABSTRACTFish vaccination aims to induce a specific immune response indicated by an increase of antibodies in vaccinated fish. However, in accordance with time the presence of antibodies will continue to decline. The purpose of this study was to determine the kinetics of specifik immune response and trend mortality against Aeromonas hydrophila and Streptococcus agalactiae on tilapia following vaccination with cocktail vaccine. Fish vaccinated through immersion for 30 minutes in a solution of diluted vaccine. Challenge test was performed for three periods, on day 22, 50, and 78 post-vaccination, fish were challenged with single infection of A. hydrophila 108 cfu. mL-1 and S. agalactiae 104 cfu. mL-1 and co-infection of both bacteria by intraperitoneal. During rearing, the blood fish were taken for determining of serum antibodies, and its  measured by ELISA. The results showed that the concentration of specific antibodies vaccinated fish were significantly higher than the control. The basal antibody levels of A. hydrophila before vaccination were higher than S. agalactiae with OD of 0.104 and 0.069 respectively. The maximum  antibody  response  was  reached  within  70  days  of  the  A. hydrophila OD= 0.264 and 56 days against S. agalactiae OD= 0.188. The mortality rate in the control group was significantly higher than vaccinated on all types and each challenge test period. The trend of mortality due to a single infection of A. hydrophila and co-infections occur more quickly than by S. agalactiae. Lowest mortality occurred in the vaccinated group at 50 day tested challenge.Keywords: kinetics antibody, Aeromnas hydrophila, Streptococcus agalactiae, Oreochromis niloticus

Sign in / Sign up

Export Citation Format

Share Document