EFIKASI BEBERAPA SEDIAAN VAKSIN Streptococcus agalactiae-N14G UNTUK PENCEGAHAN PENYAKIT STREPTOCOCCOSIS PADA IKAN NILA, Oreochromis niloticus

Penelitian dengan tujuan untuk mengetahui efikasi berbagai sediaan (biakan cair, sel utuh, supernatan dengan dan tanpa penyaringan) vaksin Streptococcus agalactiae-N14G untuk pencegahan penyakit Streptococcosis pada ikan nila telah dilakukan pada skala laboratorium. Isolat bakteri Streptococcus agalactiae-N14G digunakan sebagai sumber antigen dalam pembuatan vaksin. Ikan nila dengan rata-rata ukuran 10-15 g/ekor dan diasumsikan bebas penyakit infeksi Streptococcus agalactiae (specific pathogen free, SPF) terhadap patogen target digunakan sebagai ikan uji. Pada hari ke-14 pasca vaksinasi, dilakukan uji tantang dengan bakteri homolog aktif pada dosis lethal (LD50), dan pengamatan dilakukan selama 21 hari. Hasil penelitian menunjukkan efikasi sediaan vaksin tertinggi diperoleh pada sediaan vaksin sel utuh (B) (76,0%), selanjutnya diikuti oleh sediaan vaksin biakan cair (A) (65,0%), sediaan vaksin supernatan tanpa penyaringan (C) (49,0%), dan sediaan vaksin dengan penyaringan (D) (36,0%); sedangkan pada kelompok kontrol positif dan negatif, masingmasing sebesar 25,0% dan 34,0%. Dua jenis sediaan vaksin masuk kategori efektif dengan nilai relative percentage survival (RPS) 8 50%, yaitu jenis vaksin sel utuh(68,00%) dan jenis sediaan vaksin biakan cair (53,37%).

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EFIKASI VAKSIN IN-AKTIF BAKTERI Aeromonas hydrophila-AHL0905-2 (HYDROVAC) dan Streptococcus agalactiae-N14G (STREPTOVAC) UNTUK PENCEGAHAN PENYAKIT BAKTERIAL PADA IKAN BUDIDAYA AIR TAWAR

Jurnal Riset Akuakultur ◽

10.15578/jra.10.4.2015.541-551 ◽

2015 ◽

Vol 10 (4) ◽

pp. 541

Author(s):

Taukhid Taukhid ◽

Uni Purwaningsih ◽

Desy Sugiani ◽

Tuti Sumiati ◽

Angela Mariana Lusiastuti

Keyword(s):

Streptococcus Agalactiae ◽

Aeromonas Hydrophila ◽

Relative Percentage ◽

Percentage Survival

Penelitian bertujuan untuk mengetahui efektivitas penggunaan vaksin hydrovac dan streptovac untuk pencegahan penyakit bakterial, motile aeromonad septicaemia (MAS) dan streptococcosis pada beberapa jenis ikan budidaya air tawar. Pengujian dilakukan pada skala laboratorium dan lapang. Jenis ikan uji yang digunakan adalah ikan lele, nila, dan gurami. Vaksinasi ikan dilakukan melalui teknik perendaman dengan dosis dan periode sesuai instruksi penggunaan yang tertera pada label produk kedua jenis vaksin tersebut. Efektivitas vaksin dievaluasi berdasarkan pendekatan nilai persen sintasan dan selanjutnya dihitung nilai relative percentage survival (RPS). Hasil penelitian diketahui bahwa nilai RPS vaksin hydrovac pada skala laboratorium pada ikan lele, nila, dan gurami masing-masing sebesar 85,45%; 65,78%; dan 52,28%. Nilai RPS yang dicapai oleh vaksin streptovac terhadap ikan nila sebesar 54,53%. Sementara, nilai RPS vaksin hydrovac pada skala lapang untuk jenis ikan lele, nila, dan gurami masing-masing 70,15%; 52,43%; dan 42,43%; sedangkan nilai RPS yang dicapai oleh vaksin streptovac adalah 40,41%.

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Efficacy of inactive bivalent and trivalent Streptococcus agalactiae bacteria (biotype 1 & 2) vaccines on tilapia, Oreochromis niloticus

E3S Web of Conferences ◽

10.1051/e3sconf/202132202014 ◽

2021 ◽

Vol 322 ◽

pp. 02014

Author(s):

Taukhid ◽

Angela M. Lusiastuti ◽

Septyan Andriyanto ◽

Desy Sugiani ◽

Tuti Sumiati ◽

...

Keyword(s):

Streptococcus Agalactiae ◽

Vaccine Efficacy ◽

Oreochromis Niloticus ◽

Challenge Test ◽

Fish Disease ◽

Post Vaccination ◽

Relative Percentage ◽

Bivalent Vaccine ◽

Main Indicator ◽

Trivalent Vaccine

Streptococcosis is a significant fish disease impacting tilapia culture in Indonesia, causing losses estimated up to IDR 15.0 billion annually. This study aims to assess the efficacy of bivalent and trivalent vaccines containing Streptococcus agalactiae bacteria on tilapia. The formula of the bivalent vaccine contains 75% of S01-196-16 and 25% of N14G isolates (v/v). Trivalent vaccine contains 30%, 35%, and 35% of N14G, NP1050, and SG01-16 isolates (v/v), respectively. A challenge test assessed the efficacy of the vaccines, and it was carried out at 30, 90, and 150 days post-vaccination by artificially infection at LD60. Selected bacteria isolate to be appointed in the challenge test are N14G (biotype 2) and S01-196-16 (biotype 1). Relative Percentage of Survival (RPS) was used as the main indicator of vaccine efficacy. The results revealed that the highest RPS of a bivalent vaccine against S. agalactiae (S01-196-16) was achieved at the first challenge (61.84%), and trivalent vaccine against S. agalactiae (N14G) and S. agalactiae (S01-196-16) was achieved at the first challenge (61.53% and 76.20%, respectively). Bivalent and trivalent S. agalactiae bacteria vaccines are promising “tools” to control streptococcosis on tilapia.

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EFIKASI VAKSIN KOMBINASI “TRIVALEN” (Aeromonas hydrophila, Streptococcus agalactiae, DAN Mycobacterium fortuitum) UNTUK PENCEGAHAN PENYAKIT BAKTERI POTENSIAL PADA BUDIDAYA IKAN AIR TAWAR

Jurnal Riset Akuakultur ◽

10.15578/jra.11.4.2016.373-385 ◽

2017 ◽

Vol 11 (4) ◽

pp. 373

Author(s):

Taukhid Taukhid ◽

Lila Gardenia ◽

Septyan Andriyanto

Keyword(s):

Streptococcus Agalactiae ◽

Aeromonas Hydrophila ◽

Mycobacterium Fortuitum ◽

Bacterial Antigen ◽

Relative Percentage ◽

Freshwater Aquaculture ◽

Percentage Survival ◽

Study Results ◽

Bacterial Antigens ◽

Combined Vaccine

Penelitian ini bertujuan untuk mengetahui efikasi vaksin “trivalen” merupakan kombinasi dari tiga jenis antigen bakteri, yaitu: Aeromonas hydrophila-AHL0905-2, Streptococcus agalactiae-N14G, dan Mycobacterium fortuitum-31 untuk pencegahan penyakit bakteri potensial pada budidaya ikan air tawar. Ikan uji menggunakan ikan lele, nila, dan gurami; di mana masing-masing jenis ikan tersebut merupakan representasi dari jenis ikan yang rentan terhadap infeksi bakteri Aeromonas hydrophila, Streptococcus agalactiae, dan Mycobacterium fortuitum. Perlakuan yang diterapkan adalah formulasi sediaan vaksin kombinasi “trivalen”, dengan komposisi sebagai berikut: (A) formulasi proporsional yang mengandung ketiga jenis antigen dengan perbandingan 1:1:1 (v/v), (B) formulasi non-proporsional yang mengandung ketiga jenis antigen dengan perbadingan 1:3:3 (v/v), dan (C) tanpa pemberian vaksin sebagai kontrol. Vaksinasi diberikan melalui perendaman dalam larutan vaksin “trivalen” pada konsentrasi bakteri 107 cfu/mL selama 30 menit. Efikasi vaksin dievaluasi berdasarkan sintasan pada akhir uji tantang terhadap bakteri patogen target, dan selanjutnya dihitung nilai relative percentage survival (RPS). Hasil penelitian menunjukkan bahwa nilai RPS vaksin “trivalen” dengan kombinasi proporsional pada ikan lele terhadap bakteri A. hydrophila-AHL0905-2, ikan nila terhadap bakteri S. agalactiae-N14G, ikan gurami terhadap bakteri M. fortuitum-31 dan A. hydrophila-AHL0905-2 masing-masing sebesar 44,61%; 43,30%; 17,86%; dan 45,00%. Nilai RPS vaksin kombinasi “trivalen” non-proporsional pada ikan lele terhadap bakteri A. hydrophila-AHL0905-2, ikan nila terhadap bakteri S. agalactiae-N14G, ikan gurami terhadap bakteri M. fortuitum-31 dan A. hydrophila-AHL0905-2 masing-masing sebesar 39,61%; 40,00%; 7,14%; dan 45,00%. Vaksin “trivalen” berpotensi sebagai sediaan vaksin yang dapat digunakan untuk pencegahan penyakit pada budidaya air tawar yang disebabkan oleh ko-infeksi lebih dari satu jenis bakteri patogen.The study aimed to determine the efficacious of combined “three-valent” vaccine composed of three bacterial antigen for the prevention of bacterial diseases on freshwater aquaculture has been carried out at laboratory level. The study used catfish, tilapia, and giant goramy; that are representative of susceptible species against Aeromonas hydrophila infection, Streptococcus agalactiae infection, and Mycobacterium fortuitum infection. The treatments applied in this study included: (A). Proporsional formula of combined “three-valent” vaccine composed of three bacterial antigens at the ratio of 1:1:1 (v/v), (B). Non-proportional formula composed of three bacterial antigens at the ratio of 1:3:3 (v/v), and (C). Un-vaccinated group as a control. The vaccine was applied through immersion at the dose of 107 cfu/mL vaccine solution for 30 minutes. Vaccine efficacious was evaluated based on survival rate after challenge test against targetted pathogens, and then the relative percentage survival (RPS) was calculated. The study results revealed that the RPS values of proportional combined vaccine on catfish against A. hydrophila-AHL0905-2, on tilapia against S. agalactiae-N14G, on goramy against M. fortuitum-31 and A. hydrophila-AHL0905-2 were 44.61%; 43.30%, 17.86%, and 45.00% respectively. RPS values of non-proportional combined vaccine on catfish against A. hydrophila-AHL0905-2, on tilapia against S. agalactiae-N14G, on goramy against M. fortuitum-31 and A. hydrophila-AHL0905-2 are 39.61%; 40.00%, 7.14%, and 45.00% respectively. “Trivalent” vaccine is a promising combined-fish vaccine which can be used for prevention of bacterial fish diseases caused by co-infection of more than one type of pathogenic bacteria.

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EFEKTIVITAS METODE APLIKASI VAKSIN TRIVALEN UNTUK PENCEGAHAN PENYAKIT BAKTERI POTENSIAL PADA BUDIDAYA IKAN AIR TAWAR

Jurnal Riset Akuakultur ◽

10.15578/jra.13.1.2018.67-76 ◽

2018 ◽

Vol 13 (1) ◽

pp. 67 ◽

Cited By ~ 1

Author(s):

Taukhid Taukhid ◽

Tuti Sumiati ◽

Septyan Andriyanto

Keyword(s):

Freshwater Fish ◽

Streptococcus Agalactiae ◽

Aeromonas Hydrophila ◽

Fish Culture ◽

Mycobacterium Fortuitum ◽

Relative Percentage ◽

Percentage Survival ◽

Test Fish ◽

Application Methods ◽

Trivalent Vaccine

Penelitian ini bertujuan untuk mendapatkan metode aplikasi vaksin trivalen yang memberikan level proteksi terbaik untuk pencegahan penyakit bakteri potensial; motile aeromonas septicemia (MAS), streptococcosis, dan mycobacteriosis pada budidaya ikan air tawar. Vaksin trivalen yang dicobakan merupakan campuran dari tiga jenis antigen, yaitu Aeromonas hydrophila-AHL0905-2 (Ah), Streptococcus agalactiae N14G (Sa), dan Mycobacterium fortuitum 31 (Mf) dengan formulasi 2 Ah : 2 Sa : 1 Mf (v/v). Ikan uji yang digunakan adalah ikan lele, nila, dan gurami; masing-masing jenis ikan tersebut merupakan representasi ikan yang rentan terhadap penyakit MAS, streptococcosis, dan mycobacteriosis. Perlakuan yang diterapkan adalah metode aplikasi dengan menggunakan vaksin trivalen melalui: (A) perendaman, (B) pakan, (C) perendaman + booster, (D) pakan + booster, dan (E) tanpa pemberian vaksin sebagai kontrol. Efektivitas metode aplikasi vaksin tersebut dievaluasi melalui nilai titer antibodi spesifik dan relative percentage survival (RPS) pasca uji tantang terhadap patogen target. Hasil penelitian menunjukkan bahwa RPS dari metode aplikasi vaksin trivalen pada ketiga jenis ikan uji yang mencapai nilai ³ 50% secara keseluruhan hanya diperoleh pada aplikasi melalui rendam + booster. Oleh karena itu, metode aplikasi tersebut dapat direkomendasikan untuk digunakan pada pembudidaya ikan air tawar.This study aims to obtain a method of application of trivalent vaccine that gives the best level of protection for prevention of potential bacterial diseases such as motile Aeromonas septicemia (MAS), streptococcosis, and ycobacteriosis in freshwater fish culture. The trivalent vaccine is a mixture of three types of antigens, namely Aeromonas hydrophila-AHL0905-2 (Ah), Streptococcus agalactiae N14G (Sa), and Mycobacterium fortuitum 31 (Mf) with the formulation of 2 Ah: 2 Sa: 1 Mf (v / v). The test fish used were catfish, tilapia, and giant gourami; each fish is a representation of fish vulnerable to MAS, streptococcosis, and mycobacteriosis. The applied treatments were the application methods of trivalent vaccine through (A) immersion, (B) feed, (C) immersion + booster, (D) feed + booster, and (E) without vaccine as the control. The effectiveness of vaccine application methods was evaluated through the value of specific antibody titer and relative percentage survival (RPS) post-challenge test against the target pathogens. The results showed that overall, the RPS value of > 50% on all three test fish species was obtained only by the vaccine application through soak + booster. Therefore, the application method can be recommended to be used in freshwater fish culture.

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Laboratory Animal Research Centre (Larc) “A Specific Pathogen Free Rodent Facility” - Covid – 19 Pandemic Response Plan

University of the Future: Re-Imagining Research and Higher Education ◽

10.29117/quarfe.2020.0285 ◽

2020 ◽

Author(s):

Muralitharan Shanmugakonar ◽

Vijay Kanth Govindharajan ◽

Kavitha Varadharajan ◽

Hamda Al-Naemi

Keyword(s):

Laboratory Animal ◽

Animal Research ◽

Research Centre ◽

Functional Requirement ◽

Specific Pathogen Free ◽

Specific Pathogen ◽

Response Plan ◽

Pandemic Response ◽

Operational Plan

Laboratory Animal Research Centre (LARC) has developed an early emergency operational plan for COVID-19 pandemic situation. Biosafety and biosecurity measures were planned and implemented ahead of time to check the functional requirement to prevent the infection. Identified necessary support for IT, transport, procurement, finance, admin and research to make the operations remotely and successfully.

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Curcumin reduces enteric isoprostane 8-iso-PGF2α and prostaglandin GF2α in specific pathogen-free Leghorn chickens challenged with Eimeria maxima

Scientific Reports ◽

10.1038/s41598-021-90679-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Victor M. Petrone-Garcia ◽

Raquel Lopez-Arellano ◽

Gabriela Rodríguez Patiño ◽

Miriam Aide Castillo Rodríguez ◽

Daniel Hernandez-Patlan ◽

...

Keyword(s):

Pilot Study ◽

Broiler Chickens ◽

Solid Phase ◽

Oxidation Products ◽

Specific Pathogen Free ◽

Post Challenge ◽

Eimeria Maxima ◽

Lipid Oxidation Products ◽

Challenge Control ◽

Specific Pathogen

AbstractThe purpose of this pilot study was to evaluate and determine the concentration of prostaglandin GF2α (PGF2α) and isoprostane 8‐iso‐PGF2α in plasma and intestine of specific pathogen-free (SPF) Leghorn chickens challenged with Eimeria maxima, with or without dietary supplementation of curcumin using solid‐phase microextraction and ultra‐performance liquid chromatography/tandem mass spectrometry. Eighty 1-day-old male SPF chickens were randomly allocated to one of four groups with four replicates (n = 5 chickens/replicate). Groups consisted of: (1) Control (no challenge), (2) Curcumin (no challenge), (3) Eimeria maxima (challenge), and (4) Eimeria maxima (challenge) + curcumin. At day 28 of age, all chickens in the challenge groups were orally gavaged with 40,000 sporulated E. maxima oocysts. No significant differences (P > 0.05) were observed in the groups regardless of the treatment or challenge with E. maxima. Enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days and 9 days post-challenge were significantly increased (P < 0.01) compared to the non-challenge control chickens. Interestingly, the enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days post-challenge were significantly reduced in chickens fed curcumin, compared to control chickens challenge with E. maxima. At 9 days post-challenge, only levels of isoprostane 8‐iso‐PGF2α in the enteric samples were significantly reduced in chickens challenged with E. maxima supplemented with curcumin, compared with E. maxima challenge chickens. No differences of isoprostane 8‐iso‐PGF2α or PGF2α were observed in plasma at both days of evaluation. Similarly, no significant differences were observed between the challenge control or chickens challenge with E. maxima and supplemented with curcumin at both times of evaluation. The results of this pilot study suggests that the antioxidant anti-inflammatory properties of curcumin reduced the oxidative damage and subsequent intestinal mucosal over-production of lipid oxidation products. Further studies to confirm and extend these results in broiler chickens are required.

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