Efficacy of inactive bivalent and trivalent Streptococcus agalactiae bacteria (biotype 1 & 2) vaccines on tilapia, Oreochromis niloticus

Streptococcosis is a significant fish disease impacting tilapia culture in Indonesia, causing losses estimated up to IDR 15.0 billion annually. This study aims to assess the efficacy of bivalent and trivalent vaccines containing Streptococcus agalactiae bacteria on tilapia. The formula of the bivalent vaccine contains 75% of S01-196-16 and 25% of N14G isolates (v/v). Trivalent vaccine contains 30%, 35%, and 35% of N14G, NP1050, and SG01-16 isolates (v/v), respectively. A challenge test assessed the efficacy of the vaccines, and it was carried out at 30, 90, and 150 days post-vaccination by artificially infection at LD60. Selected bacteria isolate to be appointed in the challenge test are N14G (biotype 2) and S01-196-16 (biotype 1). Relative Percentage of Survival (RPS) was used as the main indicator of vaccine efficacy. The results revealed that the highest RPS of a bivalent vaccine against S. agalactiae (S01-196-16) was achieved at the first challenge (61.84%), and trivalent vaccine against S. agalactiae (N14G) and S. agalactiae (S01-196-16) was achieved at the first challenge (61.53% and 76.20%, respectively). Bivalent and trivalent S. agalactiae bacteria vaccines are promising “tools” to control streptococcosis on tilapia.

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Efikasi Vaksinasi pada Benih Ikan Nila (Oreochromis niloticus) dengan Metode Infiltrasi Hiperosmotik untuk Mencegah Infeksi Streptococcus agalactiae (THE EFFICACY OF VACCINATION ON TILAPIA SEEDS OF (Oreochromis niloticus) USING HYPEROSMOTIC INFILTRATION

jurnal veteriner ◽

10.19087/jveteriner.2017.18.4.634 ◽

2018 ◽

Vol 18 (4) ◽

pp. 634 ◽

Cited By ~ 1

Author(s):

Amalia Putri Firdausi ◽

Sukenda Sukenda ◽

Sri Nuryati

Keyword(s):

Immune System ◽

Antibody Level ◽

Specific Antibody ◽

Streptococcus Agalactiae ◽

Oreochromis Niloticus ◽

Challenge Test ◽

Salinity Treatment ◽

Post Vaccination ◽

Randomized Design ◽

And Control

The objective of this study was to evaluate the effectiveness of vaccination on tilapia seedlings resulted from the vaccinated parent by hyperosmotic infiltration method at four different salinity to prevent Streptococcus agalactiae bacterial infection. A total of 100 seeds aged 20 days from the vaccinated mother were immersed in four different salinity: 0 ppt (control), 10 ppt, 20 ppt, and 30 ppt for five minutes, then removed and transferred into vaccine-containing containers for 30 minutes. Further maintenance was performed in freshwater aquariums and at days 10, 20, and 30 post vaccination, the immune system performance: Relative Percent Survival (RPS) after the challenge test, specific antibody level, and lysozyme was observed. The study was conducted using a complete randomized design with three replications. The results showed that the 10 ppt salinity treatment gave the best results compared to the others and control. The final RPS (10 ppt) value was 84.72%, 66.49%, and 47.06%, on the 10th, 20th, and 30thh days of vaccination, respectively. Specific antibody levels (10 ppt) were 0.077, 0.078, and 0.077 and lysozyme 0.092, 0.084, and 0.032 at days 10, 20, and 30 post vaccination, respectively. The value of RPS and specific antibody level at 10 ppt salinity was significantly different (P <0.05) compared to the other treatments, while the lysozyme treatment activity of 10 ppt was not significantly different compared to the 20 ppt treatment. Vaccination using 10 ppt saline hyperosmotic infiltration can improve the performance of the immune system by improving maternal immune protection against Streptococcus agalactiae infection.

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EFEKTIVITAS SERBUK DAUN TANAMAN KAYU MANIS (Cinnamomum burmanii) TERHADAP DIFERENSIAL LEUKOSIT DAN AKTIVITAS FAGOSITOSIS IKAN NILA (Oreochoromis niloticus) YANG DIINFEKSI Streptococcus agalactiae

Jurnal Perikanan Pantura (JPP) ◽

10.30587/jpp.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

pp. 40

Author(s):

Sri Wulandari ◽

Rahmad Jumadi ◽

Firma Fika Rahmawati

Keyword(s):

Streptococcus Agalactiae ◽

Oreochromis Niloticus ◽

Phagocytic Activity ◽

Challenge Test ◽

Bacterial Disease ◽

Leaf Powder ◽

Alternative Measures ◽

Monocyte Cell ◽

And Control ◽

Phagocytosis Activity

The main problem in the cultivation of tilapia is the attack of bacterial disease Streptococcosiscaused by Streptococcus agalactiae bacteria. Alternative measures to prevent the disease ofStreptocococis can be through the use of phytopharmaca materials. One of the ingredients ofphytopharmaca can be used is cinnamon plant. Cinnamon leaves contain several types of activesubstances such as tannins, eugenol, safrole, calcium oxalate, resin, saponins, tanners, andsinamaldehid. The objective of the study of effectiveness of leaf powder of cinnamon plant toleukocyte differential and phagocytic activity in tilapia (Oreochromis niloticus) infected byStreptococcus agalactiae is to know the influence and dosage of cinnamon leaf powder in feedtoLeukocyte Differentiation and Phagocytosis Activity Postcainfection Streptococcus agalactiaein tilapia (Oreochromis niloticus). This research used 3treatment 4 replication and control withdose K- = challenge test, K+ = without test challenge, A = dose 0,25%, B = dose 0,5%, C = 1%.The parameters observed are Leukocyte Differential and Phagocytosis Activity. Dosage 0.5%addition of leaf powder cinnamon plant on feed is the best dose. Giving of cinnamon leaf powderin feed influenced to increase of leukocyte difference especially on monocyte cell and neutrophilcell was significantly different (P>0,05) than K+ without addition of cinnamon leaf powderwhile phagocytic activity had an effect on Streptococcus agacatiae.

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Influence of temperature on the vaccine efficacy against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)

Aquaculture ◽

10.1016/j.aquaculture.2020.734943 ◽

2020 ◽

Vol 521 ◽

pp. 734943 ◽

Cited By ~ 2

Author(s):

Jing Wang ◽

Dan-Qi Lu ◽

Biao Jiang ◽

Xu-Bing Mo ◽

Jia-Jia Du ◽

...

Keyword(s):

Streptococcus Agalactiae ◽

Vaccine Efficacy ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Influence Of Temperature ◽

Nile Tilapia Oreochromis Niloticus ◽

Influence Of Temperature On

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EFIKASI BEBERAPA SEDIAAN VAKSIN Streptococcus agalactiae-N14G UNTUK PENCEGAHAN PENYAKIT STREPTOCOCCOSIS PADA IKAN NILA, Oreochromis niloticus

Jurnal Riset Akuakultur ◽

10.15578/jra.9.2.2014.295-305 ◽

2014 ◽

Vol 9 (2) ◽

pp. 295

Author(s):

Taukhid Taukhid ◽

Uni Purwaningsih ◽

Angela Mariana Lusiastuti

Keyword(s):

Streptococcus Agalactiae ◽

Oreochromis Niloticus ◽

Specific Pathogen Free ◽

Relative Percentage ◽

Percentage Survival ◽

Specific Pathogen

Penelitian dengan tujuan untuk mengetahui efikasi berbagai sediaan (biakan cair, sel utuh, supernatan dengan dan tanpa penyaringan) vaksin Streptococcus agalactiae-N14G untuk pencegahan penyakit Streptococcosis pada ikan nila telah dilakukan pada skala laboratorium. Isolat bakteri Streptococcus agalactiae-N14G digunakan sebagai sumber antigen dalam pembuatan vaksin. Ikan nila dengan rata-rata ukuran 10-15 g/ekor dan diasumsikan bebas penyakit infeksi Streptococcus agalactiae (specific pathogen free, SPF) terhadap patogen target digunakan sebagai ikan uji. Pada hari ke-14 pasca vaksinasi, dilakukan uji tantang dengan bakteri homolog aktif pada dosis lethal (LD50), dan pengamatan dilakukan selama 21 hari. Hasil penelitian menunjukkan efikasi sediaan vaksin tertinggi diperoleh pada sediaan vaksin sel utuh (B) (76,0%), selanjutnya diikuti oleh sediaan vaksin biakan cair (A) (65,0%), sediaan vaksin supernatan tanpa penyaringan (C) (49,0%), dan sediaan vaksin dengan penyaringan (D) (36,0%); sedangkan pada kelompok kontrol positif dan negatif, masingmasing sebesar 25,0% dan 34,0%. Dua jenis sediaan vaksin masuk kategori efektif dengan nilai relative percentage survival (RPS) 8 50%, yaitu jenis vaksin sel utuh(68,00%) dan jenis sediaan vaksin biakan cair (53,37%).

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Penggunaan bakteri probiotik untuk pencegahan infeksi bakteri Streptococcus agalactiae pada ikan Nila, Oreochromis niloticus

e-Journal BUDIDAYA PERAIRAN ◽

10.35800/bdp.6.2.2018.20556 ◽

2018 ◽

Vol 6 (2) ◽

Author(s):

Aisin Umasugi ◽

Reiny A. Tumbol ◽

Reni L. Kreckhoff ◽

Henky Manoppo ◽

Novie P.L. Pangemanan ◽

...

Keyword(s):

Statistical Analysis ◽

Survival Rate ◽

Streptococcus Agalactiae ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Challenge Test ◽

Probiotic Bacteria ◽

Growth And Survival ◽

Significant Difference ◽

Materials Used

This study aimed to evaluate the effect of probiotic on growth and survival of tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection. The test materials used were commercial probiotic bacteria and Streptococcus agalactiae. The probiotic bacteria were administered by mixing into the feed with a dose of 0 mL (without probiotics), 10 mL / kg of feed, 15 mL / kg of feed and 20 mL / kg of feed. Feed was given 3 times a day at 08.00, 12.00 and 16.00 for 21 days. After that, the challenge test was done with Streptococcus agalactiae bacteria by cohabitation. Bacteria with a density of 107 cells / mL were mixed into the water and allowed for 2 hours. The result showed that B treatment (10 mL / kg of feed) gave the best result with 93,33 percent of survival rate. Statistical analysis showed that the survival of fish in treatments B, C and D was significantly different from treatment A (P <0.1). However, there was no significant difference between treatments B, C, and D. It was also found that the addition of probiotic in feed did not affect the growth of Nile tilapia (Orechromis niloticus). Keywords : Probiotic bacteria, nile tilapia, Streptococcus agalactiae, growth, infection

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Specific Immune Response Kinetics and Mortality Patterns of Tilapia Oreochromis niloticus on Post-Cocktail Vaccination Period against the Infection of Aeromonas hydrophila and Streptococcus agalactiae

Omni-Akuatika ◽

10.20884/1.oa.2017.13.2.279 ◽

2017 ◽

Vol 13 (2) ◽

Cited By ~ 2

Author(s):

Sukenda Sukenda ◽

Tuti Sumiati ◽

Sri Nuryati ◽

Angela Mariana Lusiastuti ◽

Dendi Hidayatullah

Keyword(s):

Immune Response ◽

Streptococcus Agalactiae ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Challenge Test ◽

Specific Immune Response ◽

Single Infection ◽

Control Group ◽

Antibody Levels ◽

Kinetics Of

ABSTRACTFish vaccination aims to induce a specific immune response indicated by an increase of antibodies in vaccinated fish. However, in accordance with time the presence of antibodies will continue to decline. The purpose of this study was to determine the kinetics of specifik immune response and trend mortality against Aeromonas hydrophila and Streptococcus agalactiae on tilapia following vaccination with cocktail vaccine. Fish vaccinated through immersion for 30 minutes in a solution of diluted vaccine. Challenge test was performed for three periods, on day 22, 50, and 78 post-vaccination, fish were challenged with single infection of A. hydrophila 108 cfu. mL-1 and S. agalactiae 104 cfu. mL-1 and co-infection of both bacteria by intraperitoneal. During rearing, the blood fish were taken for determining of serum antibodies, and its measured by ELISA. The results showed that the concentration of specific antibodies vaccinated fish were significantly higher than the control. The basal antibody levels of A. hydrophila before vaccination were higher than S. agalactiae with OD of 0.104 and 0.069 respectively. The maximum antibody response was reached within 70 days of the A. hydrophila OD= 0.264 and 56 days against S. agalactiae OD= 0.188. The mortality rate in the control group was significantly higher than vaccinated on all types and each challenge test period. The trend of mortality due to a single infection of A. hydrophila and co-infections occur more quickly than by S. agalactiae. Lowest mortality occurred in the vaccinated group at 50 day tested challenge.Keywords: kinetics antibody, Aeromnas hydrophila, Streptococcus agalactiae, Oreochromis niloticus

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EFEKTIVITAS METODE APLIKASI VAKSIN TRIVALEN UNTUK PENCEGAHAN PENYAKIT BAKTERI POTENSIAL PADA BUDIDAYA IKAN AIR TAWAR

Jurnal Riset Akuakultur ◽

10.15578/jra.13.1.2018.67-76 ◽

2018 ◽

Vol 13 (1) ◽

pp. 67 ◽

Cited By ~ 1

Author(s):

Taukhid Taukhid ◽

Tuti Sumiati ◽

Septyan Andriyanto

Keyword(s):

Freshwater Fish ◽

Streptococcus Agalactiae ◽

Aeromonas Hydrophila ◽

Fish Culture ◽

Mycobacterium Fortuitum ◽

Relative Percentage ◽

Percentage Survival ◽

Test Fish ◽

Application Methods ◽

Trivalent Vaccine

Penelitian ini bertujuan untuk mendapatkan metode aplikasi vaksin trivalen yang memberikan level proteksi terbaik untuk pencegahan penyakit bakteri potensial; motile aeromonas septicemia (MAS), streptococcosis, dan mycobacteriosis pada budidaya ikan air tawar. Vaksin trivalen yang dicobakan merupakan campuran dari tiga jenis antigen, yaitu Aeromonas hydrophila-AHL0905-2 (Ah), Streptococcus agalactiae N14G (Sa), dan Mycobacterium fortuitum 31 (Mf) dengan formulasi 2 Ah : 2 Sa : 1 Mf (v/v). Ikan uji yang digunakan adalah ikan lele, nila, dan gurami; masing-masing jenis ikan tersebut merupakan representasi ikan yang rentan terhadap penyakit MAS, streptococcosis, dan mycobacteriosis. Perlakuan yang diterapkan adalah metode aplikasi dengan menggunakan vaksin trivalen melalui: (A) perendaman, (B) pakan, (C) perendaman + booster, (D) pakan + booster, dan (E) tanpa pemberian vaksin sebagai kontrol. Efektivitas metode aplikasi vaksin tersebut dievaluasi melalui nilai titer antibodi spesifik dan relative percentage survival (RPS) pasca uji tantang terhadap patogen target. Hasil penelitian menunjukkan bahwa RPS dari metode aplikasi vaksin trivalen pada ketiga jenis ikan uji yang mencapai nilai ³ 50% secara keseluruhan hanya diperoleh pada aplikasi melalui rendam + booster. Oleh karena itu, metode aplikasi tersebut dapat direkomendasikan untuk digunakan pada pembudidaya ikan air tawar.This study aims to obtain a method of application of trivalent vaccine that gives the best level of protection for prevention of potential bacterial diseases such as motile Aeromonas septicemia (MAS), streptococcosis, and ycobacteriosis in freshwater fish culture. The trivalent vaccine is a mixture of three types of antigens, namely Aeromonas hydrophila-AHL0905-2 (Ah), Streptococcus agalactiae N14G (Sa), and Mycobacterium fortuitum 31 (Mf) with the formulation of 2 Ah: 2 Sa: 1 Mf (v / v). The test fish used were catfish, tilapia, and giant gourami; each fish is a representation of fish vulnerable to MAS, streptococcosis, and mycobacteriosis. The applied treatments were the application methods of trivalent vaccine through (A) immersion, (B) feed, (C) immersion + booster, (D) feed + booster, and (E) without vaccine as the control. The effectiveness of vaccine application methods was evaluated through the value of specific antibody titer and relative percentage survival (RPS) post-challenge test against the target pathogens. The results showed that overall, the RPS value of > 50% on all three test fish species was obtained only by the vaccine application through soak + booster. Therefore, the application method can be recommended to be used in freshwater fish culture.

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Contributions of lipopolysaccharide and the type IVB secretion system to Coxiella burnetii vaccine efficacy and reactogenicity

npj Vaccines ◽

10.1038/s41541-021-00296-6 ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Carrie M. Long ◽

Paul A. Beare ◽

Diane C. Cockrell ◽

Jonathan Fintzi ◽

Mahelat Tesfamariam ◽

...

Keyword(s):

Phase I ◽

Coxiella Burnetii ◽

Vaccine Efficacy ◽

Q Fever ◽

Secretion System ◽

Cell Vaccine ◽

Post Vaccination ◽

Whole Cell Vaccine ◽

Type Ivb Secretion ◽

Type Ivb Secretion System

AbstractCoxiella burnetii is the bacterial causative agent of the zoonosis Q fever. The current human Q fever vaccine, Q-VAX®, is a fixed, whole cell vaccine (WCV) licensed solely for use in Australia. C. burnetii WCV administration is associated with a dermal hypersensitivity reaction in people with pre-existing immunity to C. burnetii, limiting wider use. Consequently, a less reactogenic vaccine is needed. Here, we investigated contributions of the C. burnetii Dot/Icm type IVB secretion system (T4BSS) and lipopolysaccharide (LPS) in protection and reactogenicity of fixed WCVs. A 32.5 kb region containing 23 dot/icm genes was deleted in the virulent Nine Mile phase I (NMI) strain and the resulting mutant was evaluated in guinea pig models of C. burnetii infection, vaccination-challenge, and post-vaccination hypersensitivity. The NMI ∆dot/icm strain was avirulent, protective as a WCV against a robust C. burnetii challenge, and displayed potentially altered reactogenicity compared to NMI. Nine Mile phase II (NMII) strains of C. burnetii that produce rough LPS, were similarly tested. NMI was significantly more protective than NMII as a WCV; however, both vaccines exhibited similar reactogenicity. Collectively, our results indicate that, like phase I LPS, the T4BSS is required for full virulence by C. burnetii. Conversely, unlike phase I LPS, the T4BSS is not required for vaccine-induced protection. LPS length does not appear to contribute to reactogenicity while the T4BSS may contribute to this response. NMI ∆dot/icm represents an avirulent phase I strain with full vaccine efficacy, illustrating the potential of genetically modified C. burnetii as improved WCVs.

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