scholarly journals INFLUENCE OF CARBON SOURCES ON A-AMYLASE PRODUCTION BY BREVIBACILLUS SP. UNDER SUBMERGED FERMENTATION

2014 ◽  
Vol 03 (02) ◽  
pp. 292-299 ◽  
Author(s):  
K.Suribabu .
Keyword(s):  
Submerged Fermentation ◽  
Carbon Sources ◽  
Amylase Production

Statistical Analysis of Growth and Amylase Production by Aspergillus flavus grown on Different Carbon Sources

2013 ◽  
Vol 2 (1) ◽  
Author(s):  
M O Oyewale
Keyword(s):  
Carbon Source ◽  
Aspergillus Flavus ◽  
Amylase Activity ◽  
Carbon Sources ◽  
Dry Weight ◽  
Soluble Starch ◽  
Optimum Growth ◽  
Amylase Production ◽  
Level Of Significance ◽  
Cassava Peel

The mycelial dry weight and dinitrosalicylic acid (D.N.S.A.) method was used to determine growth and amylase production by Aspergillus flavus grown on different carbon sources. Growth of the fungus was determined at 24 h intervals over a period of six days by the dry mycelial weight methods, while the amylase activity in the culture filtrates of A. flavus was determined by the D.N.S.A method. A total of 45 samples were prepared to determine growth and amylase activity of Aspergillus flavus grown on different carbon sources. The concentration of the various carbon sources ranges between 0.4 to 2% W/V. Duncan’s multiple range test was used to determine the level of significance of the different carbon sources for effective growth and amylase production by Aspergillus flavus. Aspergillus flavus demonstrated the capability to produce significant growth and amylase activities in the medium containing soluble starch, sorghum and cassava peel as sole carbon source. The amount of mycelial dry weight produced from soluble starch, sorghum and cassava peel is significantly higher than those produced from other carbon sources. The data revealed that there is a correlation between growth and amylase production by Aspergillus flavus. The available data from this study showed that soluble starch is the best carbon source for optimum growth and amylase production by A flavus while sorghum and cassava peel are close substitute for optimum growth and amylase production by Aspergillus flavus. Keywords: Growth, amylase activity and Aspergillus flavus

scholarly journals Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp. BCC 01-50 and Potential Applications

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Altaf Ahmed Simair ◽  
Abdul Sattar Qureshi ◽  
Imrana Khushk ◽  
Chaudhry Haider Ali ◽  
Safia Lashari ◽  
...  
Keyword(s):  
Inorganic Compounds ◽  
Synthetic Medium ◽  
Research Work ◽  
Carbon Sources ◽  
Maximal Activity ◽  
Potassium Nitrate ◽  
Time Profile ◽  
Beef Extract ◽  
Amylase Production

Amylase is an industrially important enzyme and applied in many industrial processes such as saccharification of starchy materials, food, pharmaceutical, detergent, and textile industries. This research work deals with the optimization of fermentation conditions for α-amylase production from thermophilic bacterial strain Bacillus sp. BCC 01-50 and characterization of crude amylase. The time profile of bacterial growth and amylase production was investigated in synthetic medium and maximum enzyme titer was observed after 60 h. In addition, effects of different carbon sources were tested as a substrate for amylase production and molasses was found to be the best. Various organic and inorganic compounds, potassium nitrate, ammonium chloride, sodium nitrate, urea, yeast extract, tryptone, beef extract, and peptone, were used and beef extract was found to be the best among the nitrogen sources used. Temperature, pH, agitation speed, and size of inoculum were also optimized. Highest enzyme activity was obtained when the strain was cultured in molasses medium for 60 h in shaking incubator (150 rpm) at 50°C and pH 8. Crude amylase showed maximal activity at pH 9 and 65°C. Enzyme remained stable in alkaline pH range 9-10 and 60–70°C. Crude amylase showed great potential for its application in detergent industry and saccharification of starchy materials.

scholarly journals Cold Active Amylases Producing Psychrotolerants Isolated from Nella Lake, Antarctica

2018 ◽  
Vol 15 (1) ◽  
pp. 05-16
Author(s):  
Abhas Kumar Maharana ◽  
Shiv Mohan Singh
Keyword(s):  
Sediment Core ◽  
Yeast Extract ◽  
Submerged Fermentation ◽  
Soluble Starch ◽  
East Antarctica ◽  
Core Samples ◽  
Larsemann Hills ◽  
Amylase Production ◽  
Cold Active ◽  
Food And Beverage

Cold active amylase was investigated by bacteria and yeast isolates from the sediment core samples of Nella Lake,Larsemann Hills region, East Antarctica. Between potential yeast and bacteria isolates screened for amylases, best isolates were identified asRhodotorula sp. Y-37 and ArthrobacteralpinusN16 by molecular technique.Amylase production capabilities of both the isolate subjected for optimization processes by using submerged fermentation technique with soluble starch as substrate.The results indicate that a supplement of 1% w/v glucose, 1% w/v yeast extract and 0.1% w/v KCl at pH 7.0with 5% v/v inoculum enhances the amylase production by 5.72-fold using Rhodotorula sp. Y-37. In other hands, the activators are 1% w/v of galactose and peptone, 0.1% w/v KCl and 2.5% v/v inoculum at pH 7.0 enhances the amylase production by 3.74-fold using ArthrobacteralpinusN16. Cold-active amylasecan be used in detergent, textile, food and beverage industries. Bio-degradation of starchy materials by cold active amylases can contribute in cleaning of environment at cold regions without harming the climate.

scholarly journals Ligninolytic enzyme production by Lenzites betulinus on selected plant raw materials

2011 ◽  
pp. 333-338
Author(s):  
Aleksandar Knezevic ◽  
Ivan Milovanovic ◽  
Mirjana Stajic ◽  
Jelena Vukojevic
Keyword(s):  
Solid State ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Peroxidase Activity ◽  
Submerged Fermentation ◽  
Laccase Activity ◽  
Raw Materials ◽  
Carbon Sources ◽  
Ligninolytic Enzyme ◽  
Plant Residues

To get a better insight into the ligninolytic system of Lenzites betulinus, the effect of wheat straw and oak sawdust, as carbon sources, on production of Mn-oxidizing peroxidases and laccase, under solid-state and submerged fermentation, was studied. Obtained results revealed considerable differences related to the both factors affecting enzyme activities. Wheat straw was more favorable carbon source for Mn-oxidizing peroxidases and oak sawdust for laccase activity. Solid-state fermentation of wheat straw was optimal for Mn-dependent peroxidase activity (72.1 Ul-1). In contrary to this, submerged fermentation of the same residue gave the highest level of versatile peroxidase activity (25.4 Ul-1). The peak of laccase activity was noted during solid-state fermentation of oak sawdust (32.3 Ul-1), while this enzyme was not detected under submerged fermentation of any plant residues.

Response Surface Methodology for the Optimization of α-Amylase Production by Bacillus subtilis ZJF-1A5

2011 ◽  
Vol 236-238 ◽  
pp. 2323-2326 ◽  
Author(s):  
Jun Liang Sun ◽  
Xin Hong Liang ◽  
Jie Zeng ◽  
Guang Lei Li ◽  
Jun Liang Sun
Keyword(s):  
Response Surface Methodology ◽  
Bacillus Subtilis ◽  
Response Surface ◽  
Process Parameters ◽  
Submerged Fermentation ◽  
Enzyme Production ◽  
Statistical Approach ◽  
Alpha Amylase ◽  
Positive Interaction ◽  
Amylase Production

The aim of this work was to optimize the process parameters through the statistical approach for the production of alpha amylase by Bacillus subtilis ZJF-1A5 in submerged fermentation (SMF). The process parameters influencing the enzyme production were identified by using response surface methodology. Among the variables screened, the temperature and time were most significant and showed a positive interaction. The optimum levels were: temperature 35.8 °C, pH 5.03 and time 54h and under these conditions α-amylase yield was up to 191.15 U/mL.

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