scholarly journals Effect of nitrate concentration on growth of green microalga Haematococcus pluvialis under laboratory conditions

2013 ◽  
Vol 35 (2) ◽  
Author(s):  
Le Thi Thom ◽  
Luu Thi Tam ◽  
Dinh Ngoc Mai ◽  
Hoang Thi Lan Anh ◽  
Ngo Thi Hoai Thu ◽  
...  
Keyword(s):  
Haematococcus Pluvialis ◽  
Nitrate Concentration ◽  
Laboratory Conditions ◽  
Green Microalga

scholarly journals Identification and Content of Astaxanthin and Its Esters from Microalgae Haematococcus pluvialis by HPLC-DAD and LC-QTOF-MS after Extraction with Various Solvents

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2413
Author(s):  
Biljana Todorović ◽  
Veno Jaša Grujić ◽  
Andreja Urbanek Krajnc ◽  
Roman Kranvogl ◽  
Jana Ambrožič-Dolinšek
Keyword(s):  
High Performance ◽  
Haematococcus Pluvialis ◽  
Methyl Tert Butyl Ether ◽  
Diode Array ◽  
Diode Array Detection ◽  
Butyl Ether ◽  
Flight Mass Spectrometry ◽  
Green Microalga ◽  
Array Detection ◽  
Long Chain

Haematococcus pluvialis, a unicellular green microalga that produces a secondary metabolite under stress conditions, bears one of the most potent antioxidants, namely xanthophyll astaxanthin. The aim of our study was to determine the content of astaxanthin and its esterified forms using three different solvents—methyl tert-butyl ether (MTBE), hexane isopropanol (HEX -IPA) and acetone (ACE)—and to identify them by using high performance liquid chromatography coupled with diode array detection and the quadrupole time-of-flight mass spectrometry (HPLC-DAD and LC-QTOF-MS) technique. We identified eleven astaxanthin monoesters, which accounted for 78.8% of the total astaxanthin pool, six astaxanthin diesters (20.5% of total), while free astaxanthin represented the smallest fraction (0.7%). Astaxanthin monoesters (C16:2, C16:1, C16:0), which were the major bioactive compounds in the H. pluvialis samples studied, ranged from 10.2 to 11.8 mg g−1 DW. Astaxanthin diesters (C18:4/C18:3, C18:1/C18:3) were detected in the range between 2.3 and 2.6 mg g−1 DW. All three solvents were found to be effective for extraction, but MTBE and hexane-isopropanol extracted the greatest amount of free bioactive astaxanthin. Furthermore, MTBE extracted more low-chain astaxanthin monoesters (C16), and hexane-isopropanol extracted more long-chain monoesters (C18 and above) and more diesters. We can conclude that MTBE is the solvent of choice for the extraction of monoesters and hexane-isopropanol for diesters.

scholarly journals Astaxanthin accumulation in the green microalga Haematococcus pluvialis: Effect of initial phosphate concentration and stepwise/continuous light stress

2020 ◽  
Vol 28 ◽  
pp. e00538
Author(s):  
Vinoj Chamilka Liyanaarachchi ◽  
Gannoru Kankanamalage Sanuji Hasara Nishshanka ◽  
Rankoth Gedara Malith Malsha Premaratne ◽  
Thilini Udayangani Ariyadasa ◽  
Pemaththu Hewa Viraj Nimarshana ◽  
...  
Keyword(s):  
Haematococcus Pluvialis ◽  
Light Stress ◽  
Continuous Light ◽  
Phosphate Concentration ◽  
Green Microalga

scholarly journals Electric Stimulation of Astaxanthin Biosynthesis in Haematococcus pluvialis

2021 ◽  
Vol 11 (8) ◽  
pp. 3348
Author(s):  
Hana Nur Fitriana ◽  
Soo Youn Lee ◽  
Sun-A Choi ◽  
Jiye Lee ◽  
Bolam Kim ◽  
...  
Keyword(s):  
Electric Stimulation ◽  
Defense Mechanism ◽  
Haematococcus Pluvialis ◽  
Bioelectrochemical System ◽  
Voltage Range ◽  
Electric Treatment ◽  
Green Microalga ◽  
Astaxanthin Production ◽  
Astaxanthin Content ◽  
Reduction Current

The green microalga Haematococcus pluvialis accumulates astaxanthin, a potent antioxidant pigment, as a defense mechanism against environmental stresses. In this study, we investigated the technical feasibility of a stress-based method for inducing astaxanthin biosynthesis in H. pluvialis using electric stimulation in a two-chamber bioelectrochemical system. When a cathodic (reduction) current of 3 mA (voltage: 2 V) was applied to H. pluvialis cells for two days, considerable lysis and breakage of algal cells were observed, possibly owing to the formation of excess reactive oxygen species at the cathode. Conversely, in the absence of cell breakage, the application of anodic (oxidation) current effectively stimulated astaxanthin biosynthesis at a voltage range of 2–6 V, whereas the same could not be induced in the untreated control. At an optimal voltage of 4 V (anodic current: 30 mA), the astaxanthin content in the cells electro-treated for 2 h was 36.9% higher than that in untreated cells. Our findings suggest that electric treatment can be used to improve astaxanthin production in H. pluvialis culture if bioelectrochemical parameters, such as electric strength and duration, are regulated properly.

scholarly journals Combined effects of nitrate concentration and illumination conditions on the growth of microalga Haematococcus pluvialis

2013 ◽  
Vol 34 (4) ◽  
Author(s):  
Dang Diem Hong ◽  
Dinh Thi Ngoc Mai ◽  
Bui Dinh Lam ◽  
Luu Thi Tam ◽  
Nguyen Thi Thu Thuy ◽  
...  
Keyword(s):  
Haematococcus Pluvialis ◽  
Nitrate Concentration ◽  
Combined Effects

scholarly journals Mixotrophic Growth of Astaxanthin-Rich Alga Haematococcus pluvialis using Refined Crude Glycerol as Carbon Substrate: Batch and Fed-Batch Cultivations

2021 ◽  
Vol 18 (2) ◽  
Author(s):  
Pongsathorn DECHATIWONGSE ◽  
Wanna CHOORIT
Keyword(s):  
Crude Glycerol ◽  
Haematococcus Pluvialis ◽  
Exponential Growth Phase ◽  
Carbon Substrate ◽  
Fed Batch ◽  
Bg11 Medium ◽  
Green Microalga ◽  
Green Stage ◽  
A Current ◽  
Mixotrophic Conditions

Due to a current overabundance of crude glycerol produced from the biodiesel industry, the compound has the potential to be used as an inexpensive carbon source for growing the green microalga Haematococcus pluvialis (H. pluvialis), the richest source of natural astaxanthin (ATX). In order to investigate the practical use of crude glycerol, microalgal cultures were grown mixotrophically and heterotrophically in BG11 medium with the supplementation of refined crude glycerol, a mixture of glycerol and ethanol, under 2, 5 and 7 g L-1, using photoautotrophic cultivation as a control. H. pluvialis green-stage growth and red-stage ATX accumulation were effectively facilitated by mixotrophic conditions, with the highest µ of 0.27 ± 0.03 day-1 and the highest ATX content of (3.5 ± 0.4 % wt) (both observed under 7 g glycerol L-1). In contrast, growth was completely inhibited under heterotrophic conditions. Under repeated fed-batch operation, the exponential growth phase, during green-stage mixotrophic cultivation, was significantly extended from 5 days (in batch) to 24 days, making biomass yield of 1.86 ± 0.06 g DCW L-1 (around 2.6-fold higher). Monitoring of substrates (glycerol, ethanol and nitrate) in the broth was carried out and subsequently suggested that further optimization of media could be made.

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