scholarly journals Biodegradation of 2,4-dichlorophenoxyacetic acid and 4-chlorophenol in contaminated soils by Pseudomonas fluorescens strain HH

2019 ◽  
Vol 41 (3) ◽  
Author(s):  
Ha Danh Duc ◽  
Nguyen Thi Oanh ◽  
Nguyen Gia Hien
Keyword(s):  
Pseudomonas Fluorescens ◽  
Contaminated Soils ◽  
Degradation Pathway ◽  
Chemical Degradation ◽  
Dichlorophenoxyacetic Acid ◽  
Loamy Soil ◽  
Moisture Contents ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Autochthonous Microorganisms

Herbicides with 2,4-dichlorophenoxyacetic acid (2,4D) has been commonly used to control weeds and widely detected in environments. In this study, biodegradating activity of Pseudomonas fluorescens HH on 2,4D and 4-chlorophenol (4CP) in soil was carried out. The inoculation with Pseudomonas fluorescens HH in soils increased the degradation of 4CP and 2,4D by from 47.0% to 51.4% and from 38.4% to 47.4%, respectively, compared to the degradation by autochthonous microorganisms. Pseudomonas fluorescens HH could degrade well 2,4D and 4CP in various soils, but the most efficient chemical removal was observed when they were in the loamy soil. Moreover, the efficiency of chemical degradation was significantly affected by the moisture contents with the highest performance of degradation at 10 and 20% soil moisture. Also, the addition of nitrogen (N), phosphorous (P) and potassium (K) stimulated the dissipation rates. The determination of degradation pathway for 2,4D in Pseudomonas fluorescens HH indicated that 2,4-dichlorophenol (2,4DCP) and 4CP were formed as metabolites.

scholarly journals Biodegradation of 2,4-dichlorophenoxyacetic acid and 4-chlorophenol in contaminated soils by Pseudomonas fluorescens strain HH

2019 ◽  
Vol 41 (3) ◽  
Author(s):  
Ha Danh Duc ◽  
Nguyen Thi Oanh ◽  
Nguyen Gia Hien
Keyword(s):  
Pseudomonas Fluorescens ◽  
Contaminated Soils ◽  
Degradation Pathway ◽  
Chemical Degradation ◽  
Dichlorophenoxyacetic Acid ◽  
Loamy Soil ◽  
Moisture Contents ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Autochthonous Microorganisms

Herbicides with 2,4-dichlorophenoxyacetic acid (2,4D) has been commonly used to control weeds and widely detected in environments. In this study, biodegradating activity of Pseudomonas fluorescens HH on 2,4D and 4-chlorophenol (4CP) in soil was carried out. The inoculation with Pseudomonas fluorescens HH in soils increased the degradation of 4CP and 2,4D by from 47.0% to 51.4% and from 38.4% to 47.4%, respectively, compared to the degradation by autochthonous microorganisms. Pseudomonas fluorescens HH could degrade well 2,4D and 4CP in various soils, but the most efficient chemical removal was observed when they were in the loamy soil. Moreover, the efficiency of chemical degradation was significantly affected by the moisture contents with the highest performance of degradation at 10 and 20% soil moisture. Also, the addition of nitrogen (N), phosphorous (P) and potassium (K) stimulated the dissipation rates. The determination of degradation pathway for 2,4D in Pseudomonas fluorescens HH indicated that 2,4-dichlorophenol (2,4DCP) and 4CP were formed as metabolites.

DEGRADATION OF 2,4-DICHLOROPHENOXYACETIC ACID BY Pseudomonas fluorescens Strain HH

2018 ◽  
Vol 40 (3) ◽  
Author(s):  
Nguyen Thi Oanh ◽  
Ha Danh Duc ◽  
Tran Dat Huy ◽  
Nguyen Gia Hien ◽  
Nguyen Thi Huynh Nhu
Keyword(s):  
Pseudomonas Fluorescens ◽  
Freeze Drying ◽  
Exponential Growth Phase ◽  
Dichlorophenoxyacetic Acid ◽  
Drying Process ◽  
Suspension Cells ◽  
Edwards Model ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Cell Growth Kinetics

Pseudomonas fluorescens HH isolated from soil utilized 2,4-Dichlorophenoxyacetic acid (2,4D) as a sole carbon and energy source. The strain completely utilized 1.0 mM of 2,4D within 30 hr. The immobilized Pseudomonas fluorescens HH degraded 2,4D with higher rates compared to the rates of free-suspension cells. The determination of degradation and cell growth kinetics in exponential growth phase of bacteria showed that both fitted with the Edwards model, in which the maximal utilization rates and inhibition coefficient were 0.079 ± 0.008 mM/h and 0.820 ± 0.03 mM, respectively. The addition of glycerol as a cryoprotectant into alginate increased the survival of bacteria in beads during freeze-drying process, which resulted in reducing the adverse effects of bead lyophilization.

scholarly journals Electroanalytical Methodology for the Direct Determination of 2,4-Dichlorophenoxyacetic Acid in Soil Samples Using a Graphite-Polyurethane Electrode

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Fernanda Ramos de Andrade ◽  
Renata Alves de Toledo ◽  
Carlos Manoel Pedro Vaz
Keyword(s):  
Irreversible Process ◽  
Direct Determination ◽  
Parameters Optimization ◽  
Dichlorophenoxyacetic Acid ◽  
Reduction Peak ◽  
Undisturbed Soil ◽  
Polyurethane Composite ◽  
Experimental Parameters ◽  
2,4 Dichlorophenoxyacetic Acid

An electroanalytical methodology was developed for the direct determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) using a graphite-polyurethane composite electrode and square wave voltammetry (SWV). 2,4-D exhibited one reduction peak with characteristics of an irreversible process at −0.54 V (versus Ag/AgCl), which is controlled by the diffusion of the reagent on the electrode surface. After the experimental parameters optimization (pH 2.0,f=50 s−1,a=0.50 V, andΔEi=0.03 V), analytical curves were constructed in the range of 0.66 mg L−1to 2.62  mg L−1. Detection (LD) and quantification (LQ) limits were 17.6 μg L−1and 58.6 μg L−1, respectively. The methodology was successfully applied to measure the percolation of the herbicide 2,4-D in undisturbed soil columns of different granulometric compositions.

Amplifying Bienzyme Cycle-linked Immunoassays for Determination of 2,4-Dichlorophenoxyacetic Acid

1996 ◽  
Vol 799 (1 Enzyme Engine) ◽  
pp. 519-524 ◽  
Author(s):  
FRANK F. BIER ◽  
EVA EHRENTREICH-FÕRSTER ◽  
FRIEDER W. SCHELLER
Keyword(s):  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

scholarly journals Optimization of the Conditions for Determining 2,4-D and Deltamethrin by HPLC

2020 ◽  
Vol 20 (4) ◽  
pp. 372-377
Author(s):  
Elena V. Melikhova ◽  
◽  
Olga V. Farafonova ◽  
Keyword(s):  
River Water ◽  
Chromatographic Determination ◽  
Capillary Columns ◽  
Dichlorophenoxyacetic Acid ◽  
Separate Determination ◽  
Isocratic Elution ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Gradient Mode

The paper presents the results of optimizing the conditions for the chromatographic determination of 2,4-dichlorophenoxyacetic acid and deltamethrin for two capillary columns (Supelcosil LC-18, Kromasil C-18). The modes of individual chromatography of pesticides were selected. The use of the isocratic elution mode in the joint presence of 2,4-dichlorophenoxyacetic acid and deltamethrin was shown and experimentally proved to be invalid. Three options were proposed for the conditions of the gradient mode of the separate detection of analytes in a mixture. The developed technique for the chromatographic separate determination of 2,4-D and deltamethrin with their joint presence was tested via analysis of river water and potato samples.

scholarly journals Characterization of Hapten-Protein Conjugates: Antibody Generation and Immunoassay Development for Chlorophenoxyacetic Acid Pesticides

2009 ◽  
Vol 92 (6) ◽  
pp. 1773-1779 ◽  
Author(s):  
Robin C Boro ◽  
K Vikas Singh ◽  
C Raman Suri
Keyword(s):  
Inhibitory Concentration ◽  
Dichlorophenoxyacetic Acid ◽  
Protein Conjugation ◽  
Protein Conjugates ◽  
Highly Sensitive ◽  
Ic50 Values ◽  
Chlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

Abstract The generation of specific and sensitive antibodies against small molecules is greatly dependent upon the characteristics of the hapten-protein conjugates. In this study, we report a new fluorescence-based method for the characterization of hapten-protein conjugates. The method is based on an effect promoted by hapten-protein conjugation density upon the fluorescence intensity of the intrinsic tryptophan chromophore molecules of the protein. The proposed methodology is applied to quantify the hapten-protein conjugation density for two different chlorophenoxyacetic acid pesticides, 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4-dichlorophenoxybutyric acid (2,4-DB), coupled to carrier protein. Highly sensitive anti-2,4-D and anti-2,4-DB antibodies were obtained using these well-characterized hapten-protein conjugates. The generated antibodies were used in an immunoassay format demonstrating inhibitory concentration (IC50) values equal to 30 and 7 ng/mL for 2,4-D and 2,4-DB, respectively. Linearity was observed in the concentration range between 0.1500 ng/mL with LODs around 4 and 3 ng/mL for 2,4-D and 2,4-DB, respectively, in standard water samples. The proposed method was successfully applied for the determination of the extent of hapten-protein conjugation to produce specific antibodies for immunoassay development against pesticides.

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