Genotoxicity of Endosseous Implants Using Two Cellular Lineages In Vitro

2014 ◽  
Vol 40 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Mariza Matsumoto ◽  
Hugo Nary Filho ◽  
Raquel Ferrari ◽  
Kristianne Fernandes ◽  
Ana Claudia Renno ◽  
...  
Keyword(s):  
Dna Damage ◽  
Acetic Acid ◽  
Comet Assay ◽  
Single Cell ◽  
Dental Implant ◽  
Genetic Damage ◽  
Endosseous Implants ◽  
Murine Fibroblasts ◽  
Murine Fibroblast

The genotoxic potential of corrosion eluates obtained from a single dental implant using murine fibroblasts or osteoblasts cells in vitro by the single-cell gel (comet) assay was examined. A single commercially available dental implant (Biotechnology) was eluted in a solution consisting of equal amounts of acetic acid and sodium chloride (0.1 M) for 1, 3, 7, 14, and 21 days. Murine fibroblast or osteoblast cultures were then exposed to all corrosion eluates obtained from endosseous dental implants for 30 minutes at 37°C. The results suggest that none of the eluates produced genotoxic changes in murine fibroblasts regardless of the length of exposure to the eluate. Similarly, no genotoxicity was found in osteoblasts. The results suggest that the dental implant eluates tested in this study did not induce genetic damage as depicted by the single-cell gel (comet) assay. Because DNA damage is an important event during oncogenesis, this study represents a relevant contribution to estimate the real risks to the cellular system induced by the corrosion products of a dental implant.

scholarly journals Study of DNA damage induced by dental bleaching agents in vitro

2006 ◽  
Vol 20 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Mariângela Esther Alencar Marques ◽  
Daisy Maria Fávero Salvadori
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Chinese Hamster ◽  
Dental Bleaching ◽  
Dna Breakage ◽  
The Mean ◽  
Positive Controls

Dental bleaching is a simple and conservative procedure for aesthetic restoration of vital and non-vital discolored teeth. Nevertheless, a number of studies have demonstrated the risk of tissue damage from the contact of these agents with the oral mucosa. In the current study, the genotoxic potential associated with exposure to dental bleaching agents was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary (CHO) cells in vitro were exposed to six commercial dental bleaching agents (Clarigel Gold - Dentsply; Whitespeed - Discus Dental; Nite White - Discus Dental; Magic Bleaching - Vigodent; Whiteness HP - FGM and Lase Peroxide - DMC). The results pointed out that all dental bleaching agents tested contributed to DNA damage as depicted by the mean tail moment, being the strongest effect observed with the highest dose of hydrogen peroxide (Whiteness HP and Lase Peroxide, at a 35% concentration). On the other hand, Magic Bleaching (Vigodent) induced the lowest level of DNA breakage. Negative and positive controls displayed absence and presence of DNA-damaging, respectively. Taken together, these results suggest that dental bleaching agents may be a factor that increases the level of DNA damage. A higher concentration of hydrogen peroxide produced higher noxious activities in the genome as detected by single cell gel (comet) assay.

scholarly journals Lack of DNA damage induced by fluoride on mouse lymphoma and human fibroblast cells by single cell gel (comet) assay

2006 ◽  
Vol 17 (2) ◽  
pp. 91-94 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Patrícia Lepage Alves de Lima ◽  
Mariângela Esther Alencar Marques ◽  
Daisy Maria Fávero Salvadori
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Human Fibroblast ◽  
Fibroblast Cells ◽  
Potential Health ◽  
Human Fibroblast Cells ◽  
Genotoxicity Tests ◽  
Mouse Lymphoma

Fluoride has widely been used in Dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury on genetic apparatus. Genotoxicity tests constitute an important part of cancer research for risk assessment of potential carcinogens. In this study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Mouse lymphoma and human fibroblast cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/mL for 3 h at 37ºC. The results pointed out that NaF in all tested concentrations did not contribute to DNA damage as depicted by the mean tail moment and tail intensity for both cellular types assessed. These findings are clinically important because they represent a valuable contribution for evaluation of the potential health risk associated with exposure to agents usually used in dental practice.

scholarly journals Isolation of Mature Spinal Motor Neurons and Single-cell Analysis Using the Comet Assay of Early Low-level DNA Damage Induced In Vitro and In Vivo

2001 ◽  
Vol 49 (8) ◽  
pp. 957-972 ◽  
Author(s):  
Zhiping Liu ◽  
Lee J. Martin
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Motor Neuron ◽  
Single Cell ◽  
Motor Neurons ◽  
Ventral Horn ◽  
Motor Neuron Degeneration ◽  
Spinal Motor Neurons

We developed an isolation technique for motor neurons from adult rat spinal cord. Spinal cord enlargements were discretely microdissected into ventral horn tissue columns that were trypsin-digested and subjected to differential low-speed centrifugation to fractionate ventral horn cell types. A fraction enriched in α-motor neurons was isolated. Motor neuron enrichment was verified by immunofluorescence for choline acetyltransferase and prelabeling axon projections to skeletal muscle. Adult motor neurons were isolated from naïve rats and were exposed to oxidative agents or were isolated from rats with sciatic nerve lesions (avulsions). We tested the hypothesis, using single-cell gel electrophoresis (comet assay), that hydrogen peroxide, nitric oxide, and peroxynitrite exposure in vitro and axotomy in vivo induce DNA damage in adult motor neurons early during their degeneration. This study contributes three important developments in the study of motor neurons. It demonstrates that mature spinal motor neurons can be isolated and used for in vitro models of motor neuron degeneration. It shows that adult motor neurons can be isolated from in vivo models of motor neuron degeneration and evaluated on a single-cell basis. This study also demonstrates that the comet assay is a feasible method for measuring DNA damage in individual motor neurons. Using these methods, we conclude that motor neurons undergoing oxidative stress from reactive oxygen species and axotomy accumulate DNA damage early in their degeneration. (J Histochem Cytochem 49:957–972, 2001)

scholarly journals Biopure MTAD Induces DNA Damage but Not Cellular Death: An In Vitro Study

2009 ◽  
Vol 03 (04) ◽  
pp. 285-289 ◽  
Author(s):  
Juliana Soares Roter Marins ◽  
Luciana Moura Sassone ◽  
Daniel Araki Ribeiro
Keyword(s):  
Comet Assay ◽  
Single Cell ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Phosphate Buffer Solution ◽  
Negative Control ◽  
Control Group ◽  
Buffer Solution ◽  
Murine Fibroblasts

ABSTRACTObjectives: The purpose of this study was to evaluate the capacity of BioPure MTAD to induce genetic damage in vitro. Genotoxicity was assessed by the single cell gel (comet) assay.Methods: Chinese hamster ovary (CHO) cells or murine fibroblasts cells were exposed to increasing final concentrations ranging from 0.1 a 10%. All treatments were performed for 1 hour at 37°C. The negative control group was treated with vehicle control (phosphate buffer solution - PBS) for 1 hour at 37°C and the positive control group was treated with methylmetanesulfonate (at 1 µM) for 1 hour at 37°C.Results: Present results showed that the BioPure MTAD was able to promote DNA breakage in CHO cells only at the highest concentration tested as well as to induce significant increase in tail moment at all tested concentrations in murine fibroblasts.Conclusions: In summary, our results indicate that BioPure MTAD is a genotoxic agent as depicted by the single cell gel (comet) assay. (Eur J Dent 2009;3:285-289)

DNA damage induced by coal dust, fly and bottom ash from coal combustion evaluated using the micronucleus test and comet assay in vitro

2017 ◽  
Vol 324 ◽  
pp. 781-788 ◽  
Author(s):  
Cristina Araujo Matzenbacher ◽  
Ana Letícia Hilario Garcia ◽  
Marcela Silva dos Santos ◽  
Caroline Cardoso Nicolau ◽  
Suziane Premoli ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Coal Combustion ◽  
Micronucleus Test ◽  
Coal Dust ◽  
Bottom Ash

scholarly journals Corrigendum to: Standardisation of the in vitro comet assay: influence of lysis time and lysis solution composition on the detection of DNA damage induced by X-rays

Mutagenesis ◽  
2019 ◽  
Vol 34 (5-6) ◽  
pp. 431-431
Author(s):  
José M Enciso ◽  
Kristine B Gutzkow ◽  
Gunnar Brunborg ◽  
Ann-Karin Olsen ◽  
Adela López de Cerain ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
X Rays ◽  
Solution Composition ◽  
Lysis Time

scholarly journals Paracoccidioidomycosis: no genetic damage in human peripheral blood cells of patients assessed by single-cell gel (comet) assay

2007 ◽  
Vol 40 (4) ◽  
pp. 476-478 ◽  
Author(s):  
Renata Aparecida Martinez Antunes Ribeiro-Vieira ◽  
Daniel Araki Ribeiro ◽  
Daisy Maria Favero Salvadori ◽  
Sílvio Alencar Marques
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Human Peripheral Blood ◽  
Paracoccidioides Brasiliensis ◽  
Peripheral Blood Cells ◽  
Genetic Damage ◽  
Systemic Fungal Infection ◽  
Dna Breakage

Paracoccidioidomycosis is a systemic fungal infection caused by Paracoccidioides brasiliensis. As infectious diseases can cause DNA damage, the authors aimed at analyzing DNA breakage in peripheral blood cells of patients with paracoccidioidomycosis by using the comet assay. The results suggested that paracoccidioidomycosis does not cause genotoxicity.

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