scholarly journals Lack of DNA damage induced by fluoride on mouse lymphoma and human fibroblast cells by single cell gel (comet) assay

2006 ◽  
Vol 17 (2) ◽  
pp. 91-94 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Patrícia Lepage Alves de Lima ◽  
Mariângela Esther Alencar Marques ◽  
Daisy Maria Fávero Salvadori
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Human Fibroblast ◽  
Fibroblast Cells ◽  
Potential Health ◽  
Human Fibroblast Cells ◽  
Genotoxicity Tests ◽  
Mouse Lymphoma

Fluoride has widely been used in Dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury on genetic apparatus. Genotoxicity tests constitute an important part of cancer research for risk assessment of potential carcinogens. In this study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Mouse lymphoma and human fibroblast cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/mL for 3 h at 37ºC. The results pointed out that NaF in all tested concentrations did not contribute to DNA damage as depicted by the mean tail moment and tail intensity for both cellular types assessed. These findings are clinically important because they represent a valuable contribution for evaluation of the potential health risk associated with exposure to agents usually used in dental practice.

scholarly journals In vitro cytotoxic and non-genotoxic effects of gutta-percha solvents on mouse lymphoma cells by single cell gel (comet) assay

2006 ◽  
Vol 17 (3) ◽  
pp. 228-232 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Mariângela Esther Alencar Marques ◽  
Daisy Maria Fávero Salvador
Keyword(s):  
Comet Assay ◽  
Single Cell ◽  
Mammalian Cells ◽  
Lymphoma Cells ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Test ◽  
Gutta Percha ◽  
Mouse Lymphoma Cells ◽  
Mouse Lymphoma

Chloroform and eucalyptol are widely used in clinical dentistry as gutta-percha solvents. However, these compounds may represent a hazard to human health, especially by causing injury to genetic apparatus and/or inducing cellular death. In this study, the genotoxic and cytotoxic potentials associated with exposure to chloroform and eucalyptol were assessed on mouse lymphoma cells in vitro by the single cell gel (comet) assay and trypan blue exclusion test, respectively. Both gutta-percha solvents proved to be cytotoxic at the same levels in concentrations of 2.5, 5 and 10 muL/mL (p<0.05). On the other hand, neither of the solvents induced DNA breakage. Taken together, these results suggest that although both tested compounds (chloroform and eucalyptol) are strong cytotoxicants, it seems that they are not likely to increase the level of DNA damage on mammalian cells.

scholarly journals Study of DNA damage induced by dental bleaching agents in vitro

2006 ◽  
Vol 20 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Mariângela Esther Alencar Marques ◽  
Daisy Maria Fávero Salvadori
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Chinese Hamster ◽  
Dental Bleaching ◽  
Dna Breakage ◽  
The Mean ◽  
Positive Controls

Dental bleaching is a simple and conservative procedure for aesthetic restoration of vital and non-vital discolored teeth. Nevertheless, a number of studies have demonstrated the risk of tissue damage from the contact of these agents with the oral mucosa. In the current study, the genotoxic potential associated with exposure to dental bleaching agents was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary (CHO) cells in vitro were exposed to six commercial dental bleaching agents (Clarigel Gold - Dentsply; Whitespeed - Discus Dental; Nite White - Discus Dental; Magic Bleaching - Vigodent; Whiteness HP - FGM and Lase Peroxide - DMC). The results pointed out that all dental bleaching agents tested contributed to DNA damage as depicted by the mean tail moment, being the strongest effect observed with the highest dose of hydrogen peroxide (Whiteness HP and Lase Peroxide, at a 35% concentration). On the other hand, Magic Bleaching (Vigodent) induced the lowest level of DNA breakage. Negative and positive controls displayed absence and presence of DNA-damaging, respectively. Taken together, these results suggest that dental bleaching agents may be a factor that increases the level of DNA damage. A higher concentration of hydrogen peroxide produced higher noxious activities in the genome as detected by single cell gel (comet) assay.

scholarly journals Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro

2004 ◽  
Vol 18 (3) ◽  
pp. 192-196 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Clarissa Scolastici ◽  
Mariângela Esther Alencar Marques ◽  
Daisy Maria Fávero Salvadori
Keyword(s):  
Dna Damage ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Genetic Material ◽  
Chinese Hamster ◽  
Potential Health ◽  
Ovary Cells ◽  
The Mean ◽  
Genotoxicity Tests

Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.

Genotoxicity of Endosseous Implants Using Two Cellular Lineages In Vitro

2014 ◽  
Vol 40 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Mariza Matsumoto ◽  
Hugo Nary Filho ◽  
Raquel Ferrari ◽  
Kristianne Fernandes ◽  
Ana Claudia Renno ◽  
...  
Keyword(s):  
Dna Damage ◽  
Acetic Acid ◽  
Comet Assay ◽  
Single Cell ◽  
Dental Implant ◽  
Genetic Damage ◽  
Endosseous Implants ◽  
Murine Fibroblasts ◽  
Murine Fibroblast

The genotoxic potential of corrosion eluates obtained from a single dental implant using murine fibroblasts or osteoblasts cells in vitro by the single-cell gel (comet) assay was examined. A single commercially available dental implant (Biotechnology) was eluted in a solution consisting of equal amounts of acetic acid and sodium chloride (0.1 M) for 1, 3, 7, 14, and 21 days. Murine fibroblast or osteoblast cultures were then exposed to all corrosion eluates obtained from endosseous dental implants for 30 minutes at 37°C. The results suggest that none of the eluates produced genotoxic changes in murine fibroblasts regardless of the length of exposure to the eluate. Similarly, no genotoxicity was found in osteoblasts. The results suggest that the dental implant eluates tested in this study did not induce genetic damage as depicted by the single-cell gel (comet) assay. Because DNA damage is an important event during oncogenesis, this study represents a relevant contribution to estimate the real risks to the cellular system induced by the corrosion products of a dental implant.

scholarly journals Wound Healing Potential of Rhodomyrtus tomentosa and its Bioactive Compounds- Rhodomyrtone

2021 ◽  
pp. 262-273
Author(s):  
Evana Kamarudin ◽  
Hasseri Halim ◽  
Tengku Shahrul Anuar ◽  
Roslinah Hussain
Keyword(s):  
Wound Healing ◽  
Bioactive Compounds ◽  
Human Fibroblast ◽  
Ethanolic Extract ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells ◽  
Healing Agent ◽  
Wound Healing Activity ◽  
Rhodomyrtus Tomentosa

Aims: The present work was aimed to study the phytochemical composition of a crude ethanolic extract of Rhodomyrtus tomentosa [SERT], and the presence of rhodomyrtone and SERT's in vitro wound healing activity. Introduction: Rhodomyrtus tomentosa is native plant to southern and southeastern Asia, India, east to southern China, Taiwan, Philippines, and south to Malaysia. In the traditional Vietnamese, Chinese and Malaysia, all its part, including leaves, roots, buds, and fruits have been used. A need for a new source of wound healing agent is the call for the investigation of the potential of R. tomentosa as the source of health-promoting agent, specifically as a natural wound healing agent. Methodology: SERT was screen for its phytochemicals and the detection of rhodomyrtone using liquid chromatography–mass spectrometry, /Quadrupole time-of-flight [LC-MS/QTOF] analysis. Cell viability, cell proliferation, and migration assay were performed to examine the SERT effect's in vitro wound healing activity on human fibroblast cells [CRL-2522]. Results: The phytochemical study showed the presence of saponins, flavonoids, tannins and steroid in the crude ethanolic extract. The LC-MS analysis of crude ethanolic extract of SERT showed presents of rhodomyrtone which is one of the major compounds in the extract. SERT exhibit proliferative and migratory rate in human fibroblast cells [CRL 2522] in dose-dependent manner, which supports wound healing process. Its bioactive compounds presented wound healing activities at 0.325 up to 2.5 µg/mL. Conclusion: Both SERT and rhodomyrtone portrayed in vitro wound healing activities. Further studies to elucidate the mechanism of action of SERT and rhodomyrtone is recommended.

scholarly journals In vitro cytotoxicity of chemical preservatives on human fibroblast cells

2018 ◽  
Vol 54 (1) ◽  
Author(s):  
Daniel Gonsales Spindola ◽  
Andre Hinsberger ◽  
Valéria Maria de Souza Antunes ◽  
Luis Felipe Gomes Michelin ◽  
Claudia Bincoletto ◽  
...  
Keyword(s):  
Human Fibroblast ◽  
In Vitro Cytotoxicity ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells ◽  
Chemical Preservatives

Differential effects of phorbol ester on the in vitro invasiveness of malignant and non-malignant human fibroblast cells

1990 ◽  
Vol 142 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Rafael Fridman ◽  
Juan Carlos Lacal ◽  
Reuven Reich ◽  
Daniel R. Bonfil ◽  
Chang-Ho Ahn
Keyword(s):  
Phorbol Ester ◽  
Human Fibroblast ◽  
Fibroblast Cells ◽  
Differential Effects ◽  
Human Fibroblast Cells ◽  
In Vitro Invasiveness

scholarly journals The effect of intermediate frequency electromagnetic field at 22 kHz on human fibroblast cells in vitro

2021 ◽  
Author(s):  
Bertalan Pintér ◽  
Zsófia Szilágyi ◽  
Erika Szabó ◽  
Györgyi Kubinyi ◽  
Yves Le Drean ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Human Fibroblast ◽  
Intermediate Frequency ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells

Reactivity trends of cobalt(III) complexes towards various amino acids based on the properties of the amino acid alkyl chains

2020 ◽  
Vol 76 (7) ◽  
pp. 663-672
Author(s):  
Charmaine Arderne ◽  
Kyle Fraser Batchelor ◽  
Bhawna Uprety ◽  
Rahul Chandran ◽  
Heidi Abrahamse
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Human Fibroblast ◽  
Fibroblast Cells ◽  
Normal Human Fibroblast ◽  
Alkyl Chains ◽  
Human Fibroblast Cells ◽  
Vis Spectroscopy ◽  
Normal Human

The reactivity of the cobalt(III) complexes dichlorido[tris(2-aminoethyl)amine]cobalt(III) chloride, [CoCl2(tren)]Cl, and dichlorido(triethylenetetramine)cobalt(III) chloride, [CoCl2(trien)]Cl, towards different amino acids (L-proline, L-asparagine, L-histidine and L-aspartic acid) was explored in detail. This study presents the crystal structures of three amino acidate cobalt(III) complexes, namely, (L-prolinato-κ2 N,O)[tris(2-aminoethyl)amine-κ4 N,N′,N′′,N′′′]cobalt(III) diiodide monohydrate, [Co(C5H8NO2)(C6H18N4)]I2·H2O, I, (L-asparaginato-κ2 N,O)[tris(2-aminoethyl)amine-κ4 N,N′,N′′,N′′′]cobalt(III) chloride perchlorate, [Co(C4H7N2O3)(C6H18N4)](Cl)(ClO4), II, and (L-prolinato-κ2 N,O)(triethylenetetramine-κ4 N,N′,N′′,N′′′)cobalt(III) chloride perchlorate, [Co(C4H7N2O3)(C6H18N4)](Cl)(ClO4), V. The syntheses of the complexes were followed by characterization using UV–Vis spectroscopy of the reaction mixtures and the initial rates of reaction were obtained by calculating the slopes of absorbance versus time plots. The initial rates suggest a stronger reactivity and hence greater affinity of the cobalt(III) complexes towards basic amino acids. The biocompatibility of the complexes was also assessed by evaluating the cytotoxicity of the complexes on cultured normal human fibroblast cells (WS1) in vitro. The compounds were found to be nontoxic after 24 h of incubation at concentrations up to 25 mM.

Thermal Properties, Biodegradability and Cytotoxicity of PLA/Sericin Films

2011 ◽  
Vol 410 ◽  
pp. 86-89
Author(s):  
Suriyan Rakmae ◽  
Nitinat Suppakarn
Keyword(s):  
Thermal Properties ◽  
Human Fibroblast ◽  
Film Surface ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells

In this study, PLA/sericin films at various contents of sericin were prepared. Thermal properties, in vitro degradability and in vitro cytotoxicity of the films were characterized. The results illustrated that the incorporation of sericin into PLA matrix crucially affected thermal properties and biodegradability of the films and also enhanced human fibroblast cells attachment and proliferation on the film surface.

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