scholarly journals Optimization of the formulation of a preservation medium for grafts in view of their subsequent transplantation

2020 ◽  
Vol 13 (3) ◽  
Author(s):  
Neyla Ben Gdara ◽  
Lotfi Bitri
Keyword(s):  
Preservation Medium

scholarly journals Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium

2020 ◽  
Vol 41 (6supl2) ◽  
pp. 3155-3164
Author(s):  
Luciane de Albuquerque ◽  
◽  
Anita Marchionatti Pigatto ◽  
João Antonio Tadeu Pigatto ◽  
◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Corneal Endothelium ◽  
Cell Loss ◽  
Average Cell ◽  
Hypothermic Preservation ◽  
Scanning Electron ◽  
Preservation Medium

The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.

scholarly journals Evaluation of Copan FecalSwab as Specimen Type for Use in Xpert C. difficile Assay

2017 ◽  
Vol 55 (10) ◽  
pp. 3123-3129 ◽  
Author(s):  
Michael J. Mashock ◽  
Matthew L. Faron ◽  
Blake W. Buchan ◽  
Nathan A. Ledeboer
Keyword(s):  
Test Performance ◽  
Pcr Assay ◽  
Content Type ◽  
Molecular Assays ◽  
Specificity And Sensitivity ◽  
Collection Device ◽  
Stool Samples ◽  
Stool Specimens ◽  
Xpert Assay ◽  
Preservation Medium

ABSTRACT Liquid-based microbiology (LBM) devices incorporating flocked swabs and preservation medium ease transport of specimens and improve specimen yield compared to traditional fiber wound swabs; however, the performance of LBM collection devices has not been evaluated in many molecular assays. It is unclear how the differences in matrix and specimen loading with an LBM device will affect test performance compared to traditional collection devices. The purpose of this study was to evaluate the performance of specimens collected in FecalSwab transport medium (Copan Diagnostics, Murrieta, CA) compared to unpreserved stool using the Cepheid Xpert C. difficile assay (Cepheid, Sunnyvale, CA). Results equivalent to unpreserved stool samples were obtained when 400 μl of FecalSwab-preserved stool was employed in the Xpert assay. The positive and negative percent agreement of specimens inoculated with FecalSwab medium ( n = 281) was 97.0% (95% confidence interval [CI], 90.9 to 96.4%) and 99.4% (95% CI, 96.4 to 99.9%), respectively, compared to reference results obtained using unpreserved stool. Throughout this study, only four discrepant results occurred when comparing preserved specimens to unpreserved stool specimens in the Xpert C. difficile PCR assay. Post discrepant analysis, using the BD MAX Cdiff assay, the specificity and sensitivity both increased to 100%. The high positive and negative percent agreements observed in this study suggest that stool preserved in FecalSwab media yields equivalent results to using unpreserved stool when tested on the Xpert C. difficile assay, allowing laboratories to adopt this liquid-based microbiology collection device.

scholarly journals Brief Note: The Use of a Special Preservation Medium for the Maintenance of Platelet Viability at 4 C

Blood ◽  
1960 ◽  
Vol 15 (6) ◽  
pp. 909-917 ◽  
Author(s):  
MARIO BALDINI ◽  
SHIRLEY EBBE ◽  
WILLIAM DAMESHEK ◽  
Janet Donovan
Keyword(s):  
Phosphate Buffer ◽  
Platelet Survival ◽  
Rabbit Platelets ◽  
Preservation Medium

Abstract Viability of rabbit platelets stored for 1 to 6 days in different media at 4 C. was studied using the Cr51-platelet survival technic. Platelets stored for 24 hours in Triton-saline or DAS-gelatin had practically no viability. When platelets were stored for 24 hours in diluted plasma, significant degrees of viability were found, but this decreased rapidly in the successive 48 hours. The viability of platelets stored in diluted plasma containing inosine, adenine, glucose and phosphate buffer was maintained at significant levels over a six day period. The data presented here suggest that viability and, consequently, the hemostatic properties of platelets stored at 4 C. can be maintained over relatively long periods of time provided that loss of the ATP reserve of the cells can be prevented.

A novel tissue preservation medium for immunoperoxidase staining of skin biopsies

1992 ◽  
Vol 127 (6) ◽  
pp. 580-584 ◽  
Author(s):  
A. CARMICHAEL ◽  
G.R. COGHILL ◽  
I.A. CREE ◽  
S. GEORGE ◽  
J.G. LOWE
Keyword(s):  
Immunoperoxidase Staining ◽  
Tissue Preservation ◽  
Skin Biopsies ◽  
Preservation Medium

Chondroitin Sulfate in a New Cornea Preservation Medium

1984 ◽  
Vol 98 (1) ◽  
pp. 112-114 ◽  
Author(s):  
Herbert E. Kaufman ◽  
Emily D. Varnell ◽  
Stephen Kaufman
Keyword(s):  
Chondroitin Sulfate ◽  
Preservation Medium

Effect of preservation medium on the mechanical properties of cat bones

1989 ◽  
Vol 60 (4) ◽  
pp. 465-467 ◽  
Author(s):  
James C. H. Goh ◽  
Eng J. Ang ◽  
Kamal Bose
Keyword(s):  
Mechanical Properties ◽  
Preservation Medium

Replication of Fungi in K-Sol Corneal Preservation Medium at 4 C

1986 ◽  
Vol 104 (9) ◽  
pp. 1362-1363 ◽  
Author(s):  
D. D. Saggau ◽  
W. M. Bourne ◽  
I. R. Sinkeldam ◽  
G. D. Roberts
Keyword(s):  
Corneal Preservation ◽  
Preservation Medium

scholarly journals Konsentrasi Sukrosa Dan Agar Di Dalam Media Pelestarian In-Vitro Ubi Jalar Var. Sukuh

Agrologia ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Jane K. J Laisina
Keyword(s):  
Sweet Potato ◽  
Leaf Number ◽  
Green Leaf ◽  
Root Number ◽  
Media Composition ◽  
Ipomea Batatas ◽  
In Vitro Preservation ◽  
The Media ◽  
Preservation Medium

The objective of this research was  to obtain suitable concentration of sucrose and agar in the sweet potato (Ipomea batatas (L) Lam) in vitro preservation medium, in order to make plant grow slowly and healthy. Experiment was done in Molecular Biologi Laboratory of PAU IPB. The experiment was arranged in factorial complete random design, using sweet potato var. Sukuh in order to know the effect of sucrose (40,50,60,70 and 80 g l-1) and agar (7 and 8 g l-1) which were added 1 g l-1 hyponex fertilizer 20-20-20. The experiment was replicated four times. Data were analyzed parametrically and non-parametrically. The result of these experiments showed the suitable conservation media was 1 g l-1 hyponex 20-20-20 + 60 g l-1sucrose + 7 g l-1 agar. Threfore, the media composition MS could produce high green inter nodes number, high root number and more than two green leaf number. This experiment also showed that MS media could be replaced by a cheaper and easily found conservation medium.

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