scholarly journals Konsentrasi Sukrosa Dan Agar Di Dalam Media Pelestarian In-Vitro Ubi Jalar Var. Sukuh

Agrologia ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Jane K. J Laisina
Keyword(s):  
Sweet Potato ◽  
Leaf Number ◽  
Green Leaf ◽  
Root Number ◽  
Media Composition ◽  
Ipomea Batatas ◽  
In Vitro Preservation ◽  
The Media ◽  
Preservation Medium

The objective of this research was  to obtain suitable concentration of sucrose and agar in the sweet potato (Ipomea batatas (L) Lam) in vitro preservation medium, in order to make plant grow slowly and healthy. Experiment was done in Molecular Biologi Laboratory of PAU IPB. The experiment was arranged in factorial complete random design, using sweet potato var. Sukuh in order to know the effect of sucrose (40,50,60,70 and 80 g l-1) and agar (7 and 8 g l-1) which were added 1 g l-1 hyponex fertilizer 20-20-20. The experiment was replicated four times. Data were analyzed parametrically and non-parametrically. The result of these experiments showed the suitable conservation media was 1 g l-1 hyponex 20-20-20 + 60 g l-1sucrose + 7 g l-1 agar. Threfore, the media composition MS could produce high green inter nodes number, high root number and more than two green leaf number. This experiment also showed that MS media could be replaced by a cheaper and easily found conservation medium.

scholarly journals Pelestarian Secara In Vitro Melalui Metode Pertumbuhan Lambat Pada Beberapa Genotipe Ubi Jalar (Ipomea Batatas (L) Lam)

Agrologia ◽  
2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Jean K J Laisina
Keyword(s):  
Sweet Potato ◽  
Slow Growth ◽  
Single Factor ◽  
Randomized Design ◽  
Ipomea Batatas ◽  
In Vitro Preservation ◽  
Potato Genotype ◽  
Completely Randomized Design ◽  
Growth Preservation

The objectives of this research were to estimate responses of several sweet potato genotypes in preservation media through in vitro slow growth preservation, and to achieve cheap and accessible media. Experiment was arranged in a completely randomized design with a single factor. The experimental factor was sweet potato genotype (Sukuh, 421.34,343.15 and 2040.8). These genotype were tested in a preservation media which consisted of 15% coconut water +  30 mg/l aspirin + 50 g/l sucrose + 7 g/l agar. Experiment was replicated four times. Data was analyzed parametrically and non-parametrically. The result showed that genotype gave high responses to preservation media. Genotype Sukuh produced highest leaves while genotype 421.34 yielded highest numbers of root and internode. In the preservation medium of Genotype Sukuh through in vitro slow growth preservation, aspirin could be added to inhibit growth by increased leaf senesence. This experiment also showed that Hiponex (20:20:20) could be used as the basic media for in vitro preservation of sweet potato

scholarly journals In vitro shoot regeneration through anther culture of Brassica spp.

1970 ◽  
Vol 35 (2) ◽  
pp. 331-341 ◽  
Author(s):  
MA Sayem ◽  
M Maniruzzaman ◽  
SS Siddique ◽  
M Al-Amin
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Maximum Rate ◽  
Media Composition ◽  
Shoot Initiation ◽  
The Media

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone  DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

scholarly journals In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi)

2015 ◽  
Vol 3 (2) ◽  
pp. 72
Author(s):  
J.-Roger Bansimba Mukiese ◽  
Aimé Diamuini Ndofunsu ◽  
Freddy Bulubulu ◽  
Alexandre Mbaya Ntumbula ◽  
Sébastien Luyindula Ndiku
Keyword(s):  
Growth Regulators ◽  
Manihot Esculenta ◽  
Callus Formation ◽  
Shoot Development ◽  
Media Composition ◽  
Manihot Esculenta Crantz ◽  
Adenine Sulphate ◽  
Meristem Tip Culture ◽  
The Media

<p>Shiny dome-like structures measuring less than 1mm in length were excised aseptically from shoot tip buds of infected of two cassava (<em>Manihot esculenta</em> Crantz) local cultivars (Boma and Mpelo Nlongi) and cultivated <em>in vitro</em> in two types of media with different combination of growth hormone: Murashige and Skoog supplemented of sucrose (20 g/l), Naphtalenacetic acid (NAA, 10 μM), Ben-zylaminopurine (BAP, 0.66 μM) as well as Gibberellic acid (GA3, 0.1 μM) with 80 mg/l of Adenine sulphate and MS-free growth regulators. After four weeks, data were scored: 29.5% responding explant with callus formation and 20.5% responding explants to shoot development in the medium with growth regulators for the cultivar Boma whereas the cultivar Mpelo-Nlongi presented 5.7% and 25.7% respectively of callus formation and shoot development. The cultivar Boma presented a tendency more pronounced for the callus formation rather than with the shoot development contrary to the cultivar Mpelo-Nlongi. In regards of this experiment, it was shown that the media composition and genotype are essential factors, which influence in vitro growth, mainly the shoot development, in the culture of meristems for cassava local accessions.</p>

scholarly journals Kajian Penambahan BahanOrganik Pada Media Tanam VW Pada Organogenesis AnggrekDendrobium SecaraIn Vitro

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Siti Rahmah ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Tissue Culture ◽  
Organic Matter ◽  
Optimization Technique ◽  
Media Composition ◽  
Growth Optimization ◽  
The World ◽  
The Media ◽  
Number Of Leaves ◽  
Bean Sprouts

In vitro tissue culture is a growth optimization technique of Dendrobium orchid with according to media composition. Nutritions in the media are important for dendrobium orchid. Dendrobium orchid  include plant from orchidaceae family its spread throughout the world like indonesia. Its features are easily planted, intersest is continuous and varied, easily assembled, the flower crown is not easy to fall and wither. Research aimed at obtaining media compositions that are easily available and able to fulfill the needs of orchid plants. The research was conducted using descriptive methods to compare different trearment; Vacin & Went and VW media with adding organic matter; extract bean sprouts, potato extrac, and water coconut; wich is conducted for eight weeks after planting. The result of addition organic matter on VW media was different toward organogenesis of orchid. The average number of shoots is 1.8; the number of leaves average of 6.8 and the number of roots average of 3.6 formed from two until eight weeks after culture.Keywords: tissue culture, growing media, Dendrobium orchid, organogenesis.ABSTRAKKultur jaringan in vitro adalah salah satu teknik optimalisasi pada pertumbuhan tanaman angrek Dendrobium dengan menyesuaikan komposisi medianya. Nutrisi yang terdapat di dalam media sangat penting bagi pertumbuhan anggrek. Anggrek Dendrobium termasuk tanaman dari keluarga Orchidaceae yang penyebarannya sampai ke pelosok dunia seperti Indonesia. Keistimewaanya mudah ditanam, bunganya terus-menerus dan bermacam-macam, mudah disusun, serta mahkota bunga tidak mudah jatuh dan layu. Penelitian yang bertujuan untuk mendapatkan komposisi media yang mudah didapat dan mampu memenuhi kebutuhan tanaman anggrek. Penelitian dilakukan menggunakan metode deskriptif untuk membandingkan perlakuan media yang berbeda yaitu media Vacin & Went, dan VW dengan penambahan bahan organik; ekstrak tauge kacang hijau, ekstrak kentang, dan air kelapa muda; yang dilakukan selama delapan minggu setelah tanam. Hasil penambahan bahan organik pada media VW berbeda terhadap organogenesis eksplan anggrek. Jumlah tunas rata-rata 1,8; Jumlah daun rata-rata 6,8 dan jumlah akar rata-rata 3,6 yang terbentuk dari dua minggu setelah kultur (MSK) sampai minggu terakhir pengamatan delapan MSK.Kata kunci: kultur jaringan, media tanam, angrek Dendrobium,organogenesis.

scholarly journals Calcium content, in vitro digestibility, and bioaccessibility in leaves of spinach (Spinacia oleracea), sweet potato (Ipomea batatas), and drumstick tree (Moringa oleifera)

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 65 ◽  
Author(s):  
Jonathan C. Allen ◽  
Joseph Y. Issa ◽  
Weiting Cai
Keyword(s):  
Sweet Potato ◽  
Calcium Intake ◽  
Moringa Oleifera ◽  
Spinacia Oleracea ◽  
Calcium Content ◽  
In Vitro Digestibility ◽  
Ipomea Batatas ◽  
Drumstick Tree ◽  
Sweet Potato Leaves

Low calcium intake, poor calcium absorption, excessive calcium losses, or some combination of these factors contribute to calcium deficiency diseases. Calcium insufficiency is associated with osteoporosis, hypertension and colon cancer among other diseases. For individuals who do not have access to milk and dairy products, do not tolerate them, or prefer not to consume them, plants may be  alternative sources of calcium. However, calcium bioavailability may be low in plant foods because calcium forms complexes with oxalates, phytate and other competing minerals. The objective of this study was to compare the calcium content, digestibility and bioaccessibility in the leaves of spinach (Spinacia oleracea), sweet potato (Ipomea batatas), and drumstick tree (Moringa oleifera). Calcium content was analyzed in dry leaf powder by atomic absorption, followed by a two-stage in vitro digestion and dialysis against a mock serum solution to determine calcium digestibility and bioaccessibility. Moringa oleifera had higher calcium content than spinach and sweet potato leaves: 1.54±0.11% of dry matter for three Moringa samples (two African and one from India), and 0.99±0.001 and 1.06±0.001, respectively, in spinach and sweet potato leaves. The mean in vitro calcium digestibility was 1.62±0.08% in spinach, 3.4±0.68% in sweet potato leaves and 33.7±9.6% for Moringa. A dialysis system was designed to model bioaccessibility of calcium, revealing that bioaccessible calcium in sweet potato leaves was a non-significant 1.4 times higher and in Moringa was 9.2 to 19.4 times higher than in spinach. Therefore, the calcium contained in Moringa leaves does not appear to be associated with poorly bioavailable complexes such as oxalate. We confirmed previous reports that bioaccessibility of the calcium is low in spinach. These findings imply that increased utilization of Ipomea batatas and Moringa oleifera leaves might increase calcium intake in people in tropical and warm temperate regions where these plants grow, or these plants might become a valuable export crop.

scholarly journals Nutritional requirements for germination and in vitro development of three Orchidaceceae species in the southern Brazilian Amazon

2018 ◽  
Vol 24 (2) ◽  
pp. 87-94 ◽  
Author(s):  
Viviane Luiza Hunhoff ◽  
Lais Alves Lage ◽  
Ednamar Gabriela Palú ◽  
Willian Krause ◽  
Celice Alexandre Silva
Keyword(s):  
Activated Charcoal ◽  
Culture Media ◽  
Leaf Number ◽  
Alternative Form ◽  
Ms Medium ◽  
Root Number ◽  
Shoot Height ◽  
Full Strength ◽  
Half Strength

Tissue culture is an alternative form of producing healthy, vigorous and regular plants on a large scale. The purpose of this study was to evaluate the most efficient culture medium for in vitro plantlet germination and development of three Orchidaceae species. Seeds disinfested of three species were dispersed in distilled water and dripped into basic Murashige and Skoog (MS) medium. The experimental design was completely randomized in a factorial 3 x 4 (three species x four culture media), with 5 replications. Four treatments were established: (1) full-strength MS medium, with the full nutrient concentration (MSØ), (2) full-strength MS medium plus 0.3% activated charcoal (MSØ ACh), (3) half- strength MS medium (½ MS) and (4) half- strength MS medium with 0.3 % activated charcoal (½ MS ACh). Germination was evaluated after 15, 20, 25, 30, and 60 days. The shoot height, leaf number and length, root number and length of plantlets of the three studied species were assessed. In A. variegata, 73% germinated after 60 days in ½ MS ACh medium. In the same period, 100% of E. viparum and S. gloriosa seeds germinated in MSØ ACh medium. The plant height, leaf number and length, root number and length were significantly higher for the species A. variegata and E. viviparum in MSØ ACh medium. The culture media ½ MS and MSØ with addition of activated charcoal favored in vitro germination for the three orchid species of this study.

scholarly journals Farklı Besi Ortamlarının Tatlı Patateste in Vitro Çoğaltım Üzerine Etkil

2021 ◽  
Vol 9 (9) ◽  
pp. 1647-1652
Author(s):  
Yasin Bedrettin Karan
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Root Weight ◽  
Root Number ◽  
Plant Weight ◽  
Industrial Plants ◽  
Field Crops ◽  
The Media ◽  
Indole 3 Acetic Acid

This study was carried out in Tokat Gaziosmanpaşa University, Faculty of Agriculture, Field Crops Department, Industrial Plants Tissue Culture Laboratory in 2020. In the study, effects of different media (LS media, LS+1 mg/L Gibberellic acid (GA3), 0.1 mg/L kinetin (KIN) and LS+0.5 mg/L indole-3-acetic acid (IAA) on sweet potato genotypes (Havuc and Hatay Yerlisi) were investigated. Plant and root weights, plant and root lengths, and number of nodes and roots were determined. In this study, plants grown in cytokinin and gibberellin media had higher root number, root weight, root length, plant height, number of nodes and plant weight compared to the ones grown in auxin media. Havuc local genotype had higher values in terms of the investigated traits in all of the media studied.

scholarly journals The Effect of MS strength, pH and Sucrose Concentrations on In vitro Propagation of Okra (A belmoschus esculentus L.) from Shoot Tip Explants .

2021 ◽  
Author(s):  
Girma Gebrehiwot Woldeyes ◽  
Tileye Feyissa Senbeta ◽  
Alelegne Yeshamebel Adugna ◽  
Agegnehu Wasse Abegaz
Keyword(s):  
Activated Charcoal ◽  
Root Length ◽  
Sucrose Concentration ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Leaf Number ◽  
Root Number ◽  
Inhibiting Effect ◽  
Ph Level

Abstract BackgroundOkra (Abelmoschus esculentus) is belongs to the family Malvaceae and genus Hibiscus. Conventional propagation of okra in a large scale is limited due to bacterial, fungal, viral disease and seed dormancy. Hence, micropropagation offers great potential to increasing the commercial availability of okra. This study aimed to investigate the effect of MS strength, sucrose concentration, and pH on the in vitro propagation of A. esculentus from shoot tips. ResultsOkra seeds were sterilized with 70% alcohol, 30% (v/v) Berekina and 0.13% HgCl2 (w/v) for 5, 40 and 10 minutes respectively. The shoots were excised from in vitro germinated seedlings and transferred to full strength MS liquid medium containing 1mg/L BAP. The in vitro initiated shoot tips were transferred to different MS strength (1, ½, ⅓, ¼) medium, sucrose concentration (30 g/L, 20 g/L, 15 g/L, 10 g/L) and pH level (pH 6.6, 6.2, 5.8, 5.4, 5.0) that contained 1.5 mg/L BAP and 200 mg/L activated charcoal. For rooting, different MS strength (full, ½, ⅓, ¼), sucrose (30 g/L, 20 g/L, 15 g/L and 10 g/L) and pH (6.6, 6.2, 5.8, 5.4, 5.0) that contained 1mg/L IBA with 200 mg/L activated charcoal were used. Full strength MS medium containing 30 g/L sucrose at pH 5.8 produced the highest mean shoot number (7.93), mean leaf number (14.37) and mean length (6.4 cm) per explant. Similarly, at pH 6.2 and 6.6 maximum shoot mean number (6.53, 5.80), leaf number (12.77, 10.43) and shoot length (6.32, 5.82 cm) were produced respectively. Maximum mean root number (15.73) and root length (6.05 cm) were recorded at ½ MS strength. At pH 5.8, 6.2 and 6.6 maximum mean root number (1.77, 1.47, 1.80) and root length (1.38, 1.25, 1.39 cm) were obtained. After acclimatization, 86% of plants survived in greenhouse. ConclusionDecreasing the MS strength, sucrose concentration and pH level had an inhibiting effect on both shoot multiplication and root formation of okra. Besides increasing pH value showed inhibiting effect on shoot multiplication.

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