IMMUNOREGULATION OF BLOOD SERUM ESTRADIOL AND PROGESTERONE LEVELS IN POSTMENOPAUSAL WOMEN

Specific antibodies against estradiol (Es) and progesterone (Pg) are known to modulate blood serum concentrations of these hormones and their biological effects after immunization of animals. It was suggested that specific IgA-Es and IgA-Pg could influence on Es and Pg levels in human blood serum. The purpose of this study was to identify the suggested correlations between serum Es and Pg and specific IgA-Es and IgA-Pg in postmenopausal healthy women (HW) and breast cancer patients (BCP). The serum levels of Es, Pg, IgA-Es and IgA-Pg were studied in 226 HW and 633 BCP by means of solid-phase immunoassay. The following results were obtained. The levels of Es in BCP (0.25 nmol/l) were higher than in HW (0.16; р < 0.0001). The levels of Pg were lower (0.79 vs 0.87; р < 0.0001), and individual Pg/Es ratios were lower (3.19 vs 6.64; р < 0.0001). Individual IgA-Pg/IgA-Es ratios correlated with decrease of Es (rs = -0.15; p = 0.029), with increase in Pg (rs = 0.38; р < 0.0001), and with increased Pg/Es ratio (rs = 0.29; р < 0.0001) in healthy women. Similar correlations were determined in BCP (correspondingly: rs = -0.14, р < 0.001; rs = 0.1, р = 0.009; rs = 0.15, р < 0.0001). The decrease of Es and increase of Pg and Pg/Es in BCP were less significant than in HW: the a quotients in regression у = ах+b (y = hormones levels and x = antibodies levels) in BCP were 3 to 4-fold lower than in HW. These peciliarities of interrelations between hormones and specific antibody levels were revealed only in ER+/PR+ BCP but not in ER+/PR- and ER-/PR- BCP. In conclusion, we have confirmed a suggestion about participation of specific antibodies in regulation of steroids levels in human blood serum. The immune regulation of hormonal status was weakened in BCP.

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COMBINED EFFECT OF ANTIBODIES TO BENZO[A]PYRENE, ESTRADIOL AND PROGESTERONE UPON SEX HORMONE CONCENTRATIONS IN BLOOD SERUM OF PREGNANT WOMEN WITH CONGENITAL MALFORMATIONS OF FETUS

Medical Immunology (Russia) ◽

10.15789/1563-0625-2018-5-647-656 ◽

2018 ◽

Vol 20 (5) ◽

pp. 647-656 ◽

Cited By ~ 1

Author(s):

A. N. Glushkov ◽

K. S. Krasilnikova ◽

E. G. Polenok ◽

M. V. Kostyanko ◽

R. V. Olennikova ◽

...

Keyword(s):

Blood Serum ◽

Pregnant Women ◽

Congenital Malformations ◽

Solid Phase ◽

Biological Effects ◽

Normal Pregnancy ◽

Sex Hormone ◽

Environmental Chemical ◽

Specific Antibodies ◽

Positive Correlations

Specific antibodies against environmental chemical gene toxicants and endogenous steroid hormones are shown to modulate concentrations of these compounds in blood serum and their biological effects in experimental models. However, probable hazards of such antibodies in human teratogenesis are still unknown. In particular, potential correlations between specific serum antibodies, sex hormone levels in pregnant women, and congenital malformations in newborns are not clear. The aim of this study was to identify possible associations between occurrence of antibodies to benzo[a]pyrene, estradiol and progesterone (Bp, Es and Pg, respectively), and congenital malformations, and effects of these antibodies upon Es and Pg concentrations in blood serum of pregnant women. We have included into the study 182 women with normal pregnancy and 101 females with congenital malformations of fetus. A non-competitive solid phase immunoassay was performed using Bp, Es and Pg conjugated to bovine serum albumin as antigens. Es and Pg serum concentrations were measured using immunoassay test-systems of “Immunotech” (Moscow). Results: strong positive correlations were revealed between the levels of studied antibodies in the both groups. High IgA-Bp/IgA-Es (> 3) and IgA-Bp/IgA-Pg ratios (> 3) were associated with congenital malformations (OR = 2.2, p = 0.013 and OR = 6.8, p < 0.0001). Positive correlations were revealed between Pg/Es and IgA-Bp/IgA-Es (rS = 0.62, p < 0.0001), and IgA-Bp/IgA-Pg ratios (rS = 0.77, p < 0.0001) in cases with inborn malformations. Similar correlations were found for the women who had normal pregnancy (rS = 0.4, p = 0.0001, and rS = 0.23, p = 0.026, respectively). The Pg/Es proportion correlated with IgG-Bp/IgG-Es (rS = 0.46, p = 0.002), and with IgG-Bp/IgG-Pg ratio (rS = 0.5, p = 0.0009) in cases of malformations, but not in women with normal pregnancy. Conclusion: we have revealed novel associations between congenital malformations of fetus and ratios of IgA-Bp/IgA-Es, as well as IgA-Bp/IgA-Pg, like as positive correlations between hormonal Pg/Es proportions, and ratios of specific antibodies in pregnant women.

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Sulfamethizole functionalized graphene oxide for in-vitro separation and determination lead in blood serum of battery manufactories workers by syringe filter-dispersive- micro solid phase extraction

Analytical Methods in Environmental Chemistry Journal ◽

10.24200/amecj.v3.i04.124 ◽

2020 ◽

Vol 3 (04) ◽

pp. 17-29

Author(s):

Abhijit De ◽

S. Mojtaba Mostafavi

Keyword(s):

Blood Serum ◽

Human Blood ◽

Solid Phase ◽

Human Blood Serum ◽

Plasma Samples ◽

Lead In Blood ◽

Serum Samples ◽

Functionalized Graphene Oxide ◽

Syringe Filter

The toxic effect of lead (Pb) causes to anemia and iron deficiency in human body. So, the lead determination in blood/serum samples is very important. In this study, a novel adsorbent based on sulfamethizole functionalized on nanographene oxide (C3H10N4O2S2-NGO; SM-NGO) was used for extraction of Pb(II) from human blood, serum and plasma samples in battery manufactories workers by SF-D-µ-SPE. By procedure, 25 mg of SM-NGO mixed with 10 mL of human blood/serum or plasma samples and aspirated by 10 mL of syringe tube. After sonication of samples for 5 min, the Pb ions adsorbed based on sulfur of SM-NGO adsorbent at pH=6 and the solid phase separated by syringe coupled to Millex-FG hydrophobic PTFE membrane (0.2 µm). Then, the lead ions were back-extracted from SM-NGO/PTFE by elution phase with 0.5 mL of nitric acid solution (0.5 M). Finally, the concentrations of Pb(II) ions were determined by AT-FAAS.

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