scholarly journals Time course of lymphocyte profile after femoral bone fracture

2021 ◽  
Vol 23 (3) ◽  
pp. 569-576
Author(s):  
E. V. Davydova ◽  
M. V. Osikov ◽  
K. S. Abramov
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Blood Lymphocyte ◽  
T Regulatory Cells ◽  
Traumatic Injury ◽  
Femoral Diaphysis ◽  
Regulatory Cells ◽  
Femoral Bone ◽  
Hla Dr ◽  
Isolated Fracture

Isolated fractures of femur account for more than 10% of all road traffic injuries. Traumatic injury of femoral bone triggers a cascade of interrelated neuroendocrine reactions at systemic level, primarily at the hypothalamic-pituitary-adrenal axis, systemic response of immune system, initiated by release of tissue degradation products, cytokines and other mediators of damage into systemic blood circulation. Specific cellular reactions in response to traumatic injury to bone tissue include both innate and adaptive immune responses. In this regard, there is still scarce information on changes in blood lymphocyte subpopulations observed after closed isolated fracture of the femoral diaphysis at the middle third, before and after surgery. The aim of the present study was to evaluate the subpopulations of peripheral blood lymphocytes following closed isolated fracture of the femoral diaphysis with bone displacement in thecourse dynamics of surgical treatment, thus being required for studies in pathogenesis, development of diagnostic criteria and creating innovative treatment approaches. The study included 20 apparently healthy men and 36 men with closed isolated fracture of the femoral diaphysis of the middle third (32A and 32B, by AO/ASIF clinical classification, coded according to ICD-10 S72.3). The exclusion criteria were as follows: exacerbation of chronic comorbidities, diseases of lymphatic system and haematopoietic organs, oncological diseases, and evidence of osteoporosis. The spectrum of blood lymphocyte subsets was assessed on days 5, 7 (immediately after surgery) and on day 18 after closed isolated fracture of femoral diaphysis. We have found that, on the day 5 after IPBC along with leukocytosis in peripheral blood, the number of T-regulatory cells, cells with markers of early (CD25+) and late activation (HLA-DR+) proved to be increased, whereas representation of NK cells was decreased. On the day 7 after IPBC and immediately after surgery, leukocytosis persisted in blood, along with increased number of T-regulatory cells, CD3+ cells with early and late activation markers. On the day 18 after closed isolated fracture of the femoral diaphysis, the total numbers of leukocytes, T-lymphocytes, T-helpers, T-regulatory cells, T cells with an early activation marker are restored in peripheral blood, whereas the number of T-lymphocytes expressing HLA-DR+ molecules showed a significant increase.

scholarly journals Frequencies of CD4+ T Regulatory Cells and their CD25high and FoxP3high Subsets Augment in Peripheral Blood of Patients with Acute and Chronic Brucellosis

2014 ◽  
Vol 5 (3) ◽  
pp. 161-168 ◽  
Author(s):  
Abbas Bahador ◽  
Jamshid Hadjati ◽  
Niloofar Hassannejad ◽  
Hadi Ghazanfari ◽  
Mohammadreza Maracy ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
T Regulatory Cells ◽  
Regulatory Cells

Interleukin-16 Expression in the Peripheral Blood and CD8 T Lymphocytes After Traumatic Injury

2005 ◽  
Vol 58 (2) ◽  
pp. 252-258 ◽  
Author(s):  
Richard Shimonkevitz ◽  
JoBeth Northrop ◽  
Lisbeth Harris ◽  
Michael Craun ◽  
David Bar-Or
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Traumatic Injury ◽  
Cd8 T Lymphocytes

EXPRESSION OF HLA-DR ANTIGENS ON PERIPHERAL BLOOD T LYMPHOCYTES AND RENAL GRAFT TUBULAR EPITHELIAL CELLS IN ASSOCIATION WITH REJECION

1986 ◽  
Vol 42 (5) ◽  
pp. 479-483 ◽  
Author(s):  
Felix C. Henny ◽  
Jan J. Weening ◽  
William M. Baldwin ◽  
Paul J. Oljans ◽  
Hans J. Tanke ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Epithelial Cells ◽  
Peripheral Blood ◽  
Tubular Epithelial Cells ◽  
Renal Graft ◽  
Hla Dr

scholarly journals Regulatory T Cells in Human Autoimmune Thyroid Disease

2006 ◽  
Vol 91 (9) ◽  
pp. 3639-3646 ◽  
Author(s):  
Mónica Marazuela ◽  
María A. García-López ◽  
Nicté Figueroa-Vega ◽  
Hortensia de la Fuente ◽  
Brenda Alvarado-Sánchez ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
T Regulatory Cells ◽  
Mononuclear Cells ◽  
Thyroid Tissue ◽  
T Cell Subsets ◽  
Thyroid Cell ◽  
Regulatory Cells ◽  
Rnase Protection ◽  
Autoimmune Thyroid

Abstract Context: T regulatory cells have a key role in the pathogenesis of autoimmune diseases in different animal models. However, less information is available regarding these cells in human autoimmune thyroid diseases (AITD). Objective: The objective of the study was to analyze different regulatory T cell subsets in patients with AITD. Design: We studied by flow cytometry and immunohistochemistry different T regulatory cell subsets in peripheral blood mononuclear cells (PBMCs) and thyroid cell infiltrates from 20 patients with AITD. In addition, the function of TREG lymphocytes was assessed by cell proliferation assays. Finally, TGF-β mRNA in thyroid tissue and its in vitro synthesis by thyroid mononuclear cells (TMCs) was determined by RNase protection assay and quantitative PCR. Results: PBMCs from AITD patients showed an increased percent of CD4+ lymphocytes expressing glucocorticoid-induced TNF receptor (GITR), Foxp3, IL-10, TGF-β, and CD69 as well as CD69+CD25bright, CD69+TGF-β, and CD69+IL-10+ cells, compared with controls. TMCs from these patients showed an increased proportion of CD4+GITR+, CD4+CD69+, and CD69+ cells expressing CD25bright, GITR, and Foxp3, compared with autologous PBMCs. Furthermore, a prominent infiltration of thyroid tissue by CD69+, CD25+, and GITR+ cells, with moderate levels of Foxp3+ lymphocytes, was observed. The suppressive function of peripheral blood TREG cells was defective in AITD patients. Finally, increased levels of TGF-β mRNA were found in thyroid tissue, and thyroid cell infiltrates synthesized in vitro significant levels of TGF-β upon stimulation through CD69. Conclusions: Although T regulatory cells are abundant in inflamed thyroid tissue, they are apparently unable, in most cases, to downmodulate the autoimmune response and the tissue damage seen in AITD.

Multiple Myeloma Patients Treated with Allogeneic Stem Cell Transplantation Show an Increased Frequency of Bone Marrow-Residing CD4+Foxp3+ T Regulatory Cells.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 5206-5206
Author(s):  
Djordje Atanackovic ◽  
Yanran Cao ◽  
Christiane Faltz ◽  
Katrin Bartels ◽  
Christine Wolschke ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Allogeneic Stem Cell Transplantation ◽  
Peripheral Blood ◽  
T Regulatory Cells ◽  
Healthy Controls ◽  
Newly Diagnosed ◽  
Regulatory Cells

Abstract BACKGROUND: Immunosuppressive CD4+Foxp3+ T regulatory cells (Treg) play a vital role in immune regulation. Thus, Treg contribute to the prevention of autoimmune disease and graft-versus-host reactions following allogeneic stem cell transplantation (alloSCT) but also to the inhibition of effective anti-tumor T cell responses. It has previously been suggested that the frequency of Treg is increased in the peripheral blood of patients with multiple myeloma (MM). However, little is known about the presence of Treg in the bone marrow and it is unclear whether allogeneic stem cell transplantation might deplete Treg from this immune compartment. METHODS: In the present study, we analyzed percentages of CD4+Foxp3+ Treg as well as Treg expression of CD45RA and CCR7 in the bone marrow (BM) and in the peripheral blood of MM patients who had received alloSCT (N=42), in newly diagnosed MM patients (N=18), and in healthy controls (N=15) using flow cytometry. In addition, we performed inhibition assays in order to test the functional relevance of peripheral and BM-residing Treg. RESULTS: While newly diagnosed MM patients and healthy controls showed no significant difference in the proportions of CD4+Foxp3+ Treg in the bone marrow, percentages of BM-residing CD4+Foxp3+ T regulatory cells were markedly higher (p<0.001 and p<0.01) in patients post alloSCT (3.3±0.3%) than in normal BM (1.0±0.3%) or in BM of untreated MM patients (1.8±0.4%). In both groups of patients (p<0.05) as well as in the healthy controls (p<0.001) percentages of Treg were higher in the peripheral blood than in the bone marrow. While there were no differences regarding the percentages of peripheral Treg between the remaining groups, patients post alloSCT had higher percentages of peripheral Treg than newly diagnosed patients (5.6±0.8 vs. 3.2±0.7%, p<0.05). More than 90% of these donor-derived peripheral and BM-residing Treg expressed a memory T cell phenotype, being negative for CD45RA and CCR7. Importantly, peripheral as well as BM-residing Treg of patients post alloSCT were capable of inhibiting the proliferation of autologous non-Treg CD4+ T cells. CONCLUSION: Our study demonstrates for the first time an increased frequency of immunosuppressive Treg in the bone marrow of MM patients. Remarkably, in our patients these memory-type Treg were all donor-derived and led to an efficient replenishment of Treg in the periphery. These Treg might be necessary for the prevention of graft-versus-host disease in the transplanted MM patients, however, they might also contribute to the failure of an effective graft-versus-myeloma effect in the majority of the patients.

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