Prognosis of severe cytomegalovirus infection in newborns

Objective is to study the features of impaired activation of T and B lymphocytes in order to predicting severe cytomegalovirus infection in newborns. Materials and methods. 133 newborns with cytomegalovirus infection were examined. Immediately after diagnosing cytomegalovirus infection, all patients observed were immunologically ex amined, including assessing count of peripheral blood T and B lymphocytes, as well as their intercellular interaction by using flow cytometry immunostaining for CD3, CD3+CD28–, CD3+CD28+, CD3–CD28+, CD4, CD8, CD20, CD20+CD40+, CD28, CD40. The test was performed by using a Beckman Coulter Epics XL laser flow cytofluorometer. Depending on the condition severity, all children were divided into two groups: 1 — cytomegalovirus infection, severe form — 60 subjects (45.1%); 2 — cytomegalovirus infection, moderate form — 73 subjects (54.9%). Results of the entire set of studied indicators for cellular and humoral arms of immune system revealed statistically significant differences for the prognosis of severe cytomegalovirus infection: CD3+CD28–, CD20, CD20+CD40+, CD4. T lymphocytes with CD3+CD28+ activation markers, through which costimulating signals necessary for the activation of T helper cells are exerted cell-intrinsic features, serving as an important factor ensuring immune response. Using the “classification trees” method, we developed a differentiated approach to forecast severe cytomegalovirus infection in newborns. Systems of inequalities were obtained, four of which classify a subgroup of newborns with severe cytomegalovirus infection. The consistent application of the obtained inequalities makes it possible to isolate from the input stream of sick patients with a prognosis of the development of severe cytomegalovirus infection. The proposed diagnostic rules can be considered as screening markers for predicting a severe cytomegalovirus infection in newborns, which makes possible the timely onset of specific therapy.

Download Full-text

Imbalance of acquired immunity factors in newborns with cytomegaloviral infection depending on the severity of the disease

Russian Clinical Laboratory Diagnostics ◽

10.51620/0869-2084-2021-66-3-166-171 ◽

2021 ◽

Vol 66 (3) ◽

pp. 166-171

Author(s):

L. V. Kravchenko

Keyword(s):

B Lymphocytes ◽

Immune Status ◽

Severe Form ◽

Cytotoxic Lymphocytes ◽

Intercellular Interaction ◽

Immunological Examination ◽

Severity Of The Disease ◽

Definition Of ◽

T Helpers

Objective: to assess the imbalance of the parameters of the immune status at the time of manifestation of CMVI in newborns in order to predict the development of a severe form of the disease. Methods: 133 newborns with CMVI were examined. Depending on the severity of the condition, all children were divided into two groups: 1 - CMVI, severe form - 60 people (45.1%); 2 - CMVI, moderate form - 73 people (54.9%). An immunological examination was carried out, including the determination of T and B lymphocytes, as well as intercellular interaction between them. Using the “classification trees” method, we compared two differentiated approaches to the prediction of severe CMVI: based on a study of the relationships between T-lymphocytes (CD3+), T-helpers (CD4+), the content of cytotoxic lymphocytes (CD8+) and B lymphocytes (CD20+) and by studying the interaction of co-stimulation molecules CD28, CD3 + CD28 +, CD3-CD28 +, CD3 + CD28-. Results. In this study, it was demonstrated that the “standard immunogram”, limited to the definition of CD3+, CD4+, CD8+, CD20+ lymphocytes, shows less information in predicting the development of a severe form of the disease in this patient population than the combination of markers, including CD3 + CD28-, CD20 + CD40 +. Findings. A model for predicting a severe form of cytomegalovirus infection in newborns based on the analysis of the content of CD 28 costimulation molecules has a higher information content, which makes it possible to start specific therapy on time.

Download Full-text

The importance of immunosupression in the development of the heavy form of cytomegaloviral infection in newborn children

HIV Infection and Immunosuppressive Disorders ◽

10.22328/2077-9828-2020-12-4-108-115 ◽

2021 ◽

Vol 12 (4) ◽

pp. 108-115

Author(s):

L. V. Kravchenko

Keyword(s):

Monoclonal Antibodies ◽

B Cell ◽

Research Methods ◽

Cytomegalovirus Infection ◽

Severe Form ◽

Membrane Markers ◽

Cell Immunity ◽

Systems Of Inequalities ◽

Heavy Form ◽

Newborn Children

Objective: to study the features of disorders in T and B cell immunity and the synthesis of serum immunoglobulins of classes IgA, IgM, IgG with the aim of predicting severe cytomegalovirus infection (CMVI) in newborns.Research methods: 133 newborns with cytomegalovirus infection were examined. Lymphocytes were typed to differentiation clusters CD3, CD4, CD8, CD20 using monoclonal antibodies, IMMUNOTECH (France). The expression of membrane markers of immunocompetent cells was determined using a Beckman COULTER EpicsXLII ﬂow laser cytoﬂuorimeter. Depending on the severity of the condition, all children were divided into two groups: 1 — CMVI, severe form — 60 people (45,1%); 2 CMVI, moderate form — 73 people (54,9%).Results. Using the «classiﬁcation trees» method, we were able to develop a differentiated approach to the prognosis of a severe form of CMVI in newborn children. Systems of inequalities were obtained, four of which classify a subgroup of newborns with severe CMVI. Conclusion: the proposed diagnostic rules can be considered screening markers for prognosis of a severe form of CMVI in newborns.

Download Full-text

Relationship among T- and B-lymphocytes of the peripheral blood and thyroid tissue in patients with Graves' disease

Endocrine Abstracts ◽

10.1530/endoabs.63.p356 ◽

2019 ◽

Author(s):

Margarita A Dudina ◽

Andrey A Savchenko ◽

Sergey A Dogadin ◽

Alexandr G Borisov ◽

Igor V Kudryavcev ◽

...

Keyword(s):

B Lymphocytes ◽

Peripheral Blood ◽

Thyroid Tissue ◽

T And B Lymphocytes ◽

Graves Disease

Download Full-text

Transplantable progenitors of natural killer cells are distinct from those of T and B lymphocytes.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.83.10.3427 ◽

1986 ◽

Vol 83 (10) ◽

pp. 3427-3431 ◽

Cited By ~ 61

Author(s):

J. Hackett ◽

G. C. Bosma ◽

M. J. Bosma ◽

M. Bennett ◽

V. Kumar

Keyword(s):

Natural Killer Cells ◽

Natural Killer ◽

B Lymphocytes ◽

T And B Lymphocytes ◽

Killer Cells

Download Full-text

Differential polarization and activation dynamics of systemic T helper cell subsets after aneurysmal subarachnoid hemorrhage (SAH) and during post-SAH complications

Scientific Reports ◽

10.1038/s41598-021-92873-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Shafqat Rasul Chaudhry ◽

Ulf Dietrich Kahlert ◽

Thomas Mehari Kinfe ◽

Elmar Endl ◽

Andreas Dolf ◽

...

Keyword(s):

Subarachnoid Hemorrhage ◽

Brain Injury ◽

T Cell ◽

Cerebral Ischemia ◽

Aneurysmal Subarachnoid Hemorrhage ◽

T Cell Subsets ◽

Cd4 T Cell ◽

T Helper ◽

Activation Markers ◽

Hla Dr

AbstractAneurysmal subarachnoid hemorrhage (SAH) is associated with high morbidity and mortality. Devastating post-SAH complications, such as cerebral vasospasm (CVS), delayed cerebral ischemia or seizures to mention a few, are mainly responsible for the poor clinical outcome. Inflammation plays an indispensable role during early brain injury (EBI) and delayed brain injury (DBI) phases over which these complications arise. T helper cells are the major cytokine secreting cells of adaptive immunity that can polarize to multiple functionally unique sub-populations. Here, we investigate different CD4+ T cell subsets during EBI and DBI phases after SAH, and their dynamics during post-SAH complications. Peripheral venous blood from 15 SAH patients during EBI and DBI phases, was analyzed by multicolour flowcytometry. Different subsets of CD3+ CD4+ T cells were characterized by differential cell surface expression of CXCR3 and CCR6 into Th1, Th2, Th17, whereas Tregs were defined by CD25hiCD127lo. The analysis of activation states was done by the expression of stable activation markers CD38 and HLA-DR. Interestingly, compared to healthy controls, Tregs were significantly increased during both EBI and DBI phases. Different activation states of Tregs showed differential significant increase during EBI and DBI phases compared to controls. HLA-DR− CD38+ Tregs were significantly increased during DBI phase compared to EBI phase in SAH patients developing CVS, seizures and infections. However, HLA-DR− CD38− Tregs were significantly reduced during EBI phase in patients with cerebral ischemia (CI) compared to those without CI. HLA-DR− CD38− Th2 cells were significantly increased during EBI phase compared to controls. A significant reduction in Th17/Tregs and HLA-DR− CD38+ Th17/Tregs ratios was observed during both EBI and DBI phases compared to controls. While HLA-DR− CD38− Th17/Tregs and HLA-DR− CD38− Th1/Th2 ratios were impaired only during EBI phase compared to controls. In conclusion, CD4+ T cell subsets display dynamic and unique activation patterns after SAH and during the course of the manifestation of post-SAH complications, which may be helpful for the development of precision neurovascular care. However, to claim this, confirmatory studies with larger patient cohorts, ideally from different ethnic backgrounds, are required. Moreover, our descriptive study may be the grounds for subsequent lab endeavors to explore the underlying mechanisms of our observations.

Download Full-text