scholarly journals Fungal Diversity and Over-Represented Non-Nectariferous Plants Pollen in Honey. Case Study on Acacia Honey Authenticity, Analyzed in APHIS Laboratory

2020 ◽  
Vol 77 (2) ◽  
pp. 54
Author(s):  
Otilia BOBIŞ ◽  
Daniel Severus DEZMIREAN ◽  
Victoriţa BONTA ◽  
Adriana Cristina URCAN ◽  
Adela Ramona MOISE ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Pollen Analysis ◽  
Pollen Grains ◽  
Botanical Origin ◽  
Photodiode Array Detection ◽  
Acacia Honey ◽  
Photodiode Array ◽  
Array Detection

Pollen analysis is the basic method for the determination of the botanical and geographical origin of honey. However, the presence of over-represented pollen in honeys may lead to false results of the analysis. This can be more severe if this pollen is present in unifloral under-represented honeys of commercial importance (e.g. thyme or acacia honey). In the present study, we investigated the abundance of nectarless pollen grains on several quality characteristics in honey samples. In particular, the physic-chemical (diastase activity, electrical conductivity, sugars and HMF content) analysis were carried out in order to confirm the declared botanical origin. Spectrophotometric method was used for diastase activity determination, electrical conductivity was determined by potentiometry and chromatographic determinations for HMF content (photodiode array detection) and sugars (HPLC refractive index detection). The present study confirms that, in the case of non-nectariferous pollen presence in honeys, a second count must be made, excluding this pollen type and pollen analysis alone cannot give reliable results for the determination of the botanical origin. Consequently, pollen analysis should be combined with other analyses, especially in honeys with under-represented pollens, to give precise results for the botanical characterization and labeling of honeys.

Determination of gangliosides by high-performance liquid chromatography with photodiode-array detection

1992 ◽  
Vol 605 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Marcello Previti ◽  
Francesco Dotta ◽  
Giuseppe Mario Pontieri ◽  
Umberto Di Mario ◽  
Luisa Lenti
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection

scholarly journals Sugar Spectrum, Hydroxymethylfurfural and Diastase Activity in Honey: A Validated Approach as Indicator of Possible Adulteration

2020 ◽  
Vol 77 (2) ◽  
pp. 44
Author(s):  
Victoriţa BONTA ◽  
Daniel Severus DEZMIREAN ◽  
Liviu Alexandru MĂRGHITAŞ ◽  
Adriana Cristina URCAN ◽  
Otilia BOBIŞ
Keyword(s):  
Antimicrobial Properties ◽  
Honey Sample ◽  
Complex Matrix ◽  
Photodiode Array Detection ◽  
Physico Chemical ◽  
Photodiode Array ◽  
Array Detection ◽  
Biotechnological Processes ◽  
Refractive Index Detection

Honey is used for centuries both as nutrient and in medicine, being an exhaustless source of benefic factors for health and also for different biotechnological processes. This is a complex matrix which possesses antioxidant and antimicrobial properties due to its peroxidic activity and bioactive compounds. A fully validated approach for the determination of sugars, hydroxymethylfurfural content and diastase activity in honey samples were presented in accordance with the official methods from the International Honey Commission. Spectrophotometric method was used for diastazic activity determination and chromatographic determinations for HMF content (photodiode array detection) and sugars (refractive index detection). The limits of detection and quantification were calculated. Accuracy, precision and uncertainty were determined in the spectrophotometric and chromatographic techniques using the certified reference material. Diastase activity, sucrose content and hydroxymethylfurfural (HMF) content were easily differentiated and these parameters were used for indication of a possible adulteration of the honey. The present study confirms that, in order to have an authentic honey sample, diastazic activity must be above 8 DN (diastazic number) and HMF content less than 40 mg/kg. Sugar spectrum is different for every honey type. Consequently, physico-chemical analysis should be combined for the determination of authentic honey and proper labeling.

scholarly journals Supercritical Fluid Chromatography with Photodiode Array Detection in the Determination of Fat-Soluble Vitamins in Hemp Seed Oil and Waste Fish Oil

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 1131 ◽  
Author(s):  
Katarzyna Tyśkiewicz ◽  
Roman Gieysztor ◽  
Izabela Maziarczyk ◽  
Paweł Hodurek ◽  
Edward Rój ◽  
...  
Keyword(s):  
Fish Oil ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Seed Oil ◽  
Photodiode Array Detection ◽  
Hemp Seed ◽  
Photodiode Array ◽  
Array Detection ◽  
Fat Soluble Vitamins

scholarly journals Quantitative Determination of Pregnanes from Aerial Parts of Caralluma Species Using HPLC-UV and Identification by LC-ESI-TOF

2011 ◽  
Vol 94 (5) ◽  
pp. 1383-1390
Author(s):  
Bharathi Avula ◽  
Yatin J Shukla ◽  
Yan-Hong Wang ◽  
Ikhlas A Khan
Keyword(s):  
Quantitative Determination ◽  
Dietary Supplements ◽  
Hplc Method ◽  
Photodiode Array Detection ◽  
Plant Materials ◽  
Aerial Parts ◽  
Photodiode Array ◽  
Array Detection ◽  
Na Ions

Abstract An HPLC method was developed for the quantitative determination of five pregnane derivatives from aerial parts of Caralluma species and dietary supplements. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of five pregnane compounds were found to be in the range of 1–5 and 3–15 μg/mL, respectively, by HPLC using photodiode array detection. This method was applied to the identification of three plant materials of Caralluma species (C. fimbriata, C. umbellate, and C. attentuata) and seven dietary supplements claiming to contain C. fimbriata. An LC/MS coupled with electrospray ionization interface method was used for the identification of compounds and involved the use of [M+Na]+ ions in the positive ion mode with extracted ion chromatogram.

Sign in / Sign up

Export Citation Format

Share Document