scholarly journals In vitro plant propagation and crop improvement in Lisianthus (Lisianthus Russelianus Hook.)

2016 ◽  
Vol 73 (2) ◽  
pp. 168
Author(s):  
Rodica Pop ◽  
Maria Cantor ◽  
Erzsebet Buta ◽  
Iudita Csete
Keyword(s):  
Crop Improvement ◽  
Ornamental Plants ◽  
Basal Medium ◽  
International Market ◽  
Nodal Segments ◽  
F1 Hybrids ◽  
Vase Life ◽  
Eustoma Grandiflorum ◽  
Planting Material

Romania assists at the present time to an increase of production crops for ornamental plants and as a consequence an increased demand of planting material. Thus, improvements of the current multiplication methods are sought after. Lisianthus russelianus Hook. (Eustoma grandiflorum Grise.) is a relatively new floral crop to the international market, known for beautiful flowers of various colors and for having a long vase life. This study focused on the development of an efficient protocol for rapid regeneration of this species following known basic and applied aspects of lisianthus biotechnology but exploring new potentials. In the course of experiments conducted, for in vitro multiplication there were used nodal segments (1.5 cm) with axillary buds from three F1 hybrids ‘Echo Lavender’, ‘Flamenco White’, ‘Mirage Pastel Pink’ that were inoculated on MS basal medium supplemented with 0.50 mg 1-1 TDZ, 1.0 mg 1-1 BAP and 0.50 mg 1-1 AIA. The results show that the medium with BAP was most effective for obtaining the highest shoots number compared to medium containing TDZ. For rooting induction, two different concentrations of auxin IBA 0.5 mg 1-1 and 1.5 mg 1-1 were used simultaneously on MS basal medium. The highest roots number occurred when using 1.5 mg 1-1 IBA. Both the number of shoots and rooting regeneration were dependent on the cultivar. The highest shoots number was achieved for ’Mirage Pastel Pink’ hybrid (6.91) on the medium containing 1.0 mg 1-1 BAP and 0.50 mg 1-1 IAA.

scholarly journals In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

2014 ◽  
Vol 20 ◽  
pp. 99-108 ◽  
Author(s):  
MS Islam ◽  
MA Bari
Keyword(s):  
Medicinal Plants ◽  
Seed Production ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Nodal Segments ◽  
Ms Medium ◽  
Mentha Arvensis ◽  
Artificial Seed ◽  
Artificial Seed Production

Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion: The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations DOI: http://dx.doi.org/10.3329/jbs.v20i0.17722 J. bio-sci.  20:  99-108, 2012

scholarly journals In Vitro Propagation of Muscadine Grape by Axillary Shoot Proliferation

1990 ◽  
Vol 115 (2) ◽  
pp. 324-329 ◽  
Author(s):  
Ni Lee ◽  
Hazel Y. Wetzstein
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Vitis Rotundifolia ◽  
Shoot Production ◽  
Muscadine Grape ◽  
Better Than

Plantlets were recovered from axillary bud cultures of muscadine grape (Vitis rotundifolia, `Summit'). Nodal segments 0.5 to 1.0 cm long were cultured in Murashige and Skoog (MS) basal medium supplemented with 5, 10, 20, or 40 μm BA. Best total shoot production was obtained with 10 μm BA; with higher BA levels, shoots were unexpanded and exhibited high mortalities. MS medium supplemented with IBA enhanced rooting by increasing rooting percentage and number per plantlet. Shoots previously proliferated on medium with 5 μm BA rooted significantly better than those multiplied on 10 μM BA. Shoot vigor during rooting was greater in shoots proliferated on 5 vs. 10 μm BA. Root development was not significantly affected by liquid vs. agar-solidifted medium or shoot length. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA), 1H-indole-3-butyric acid (IBA).

scholarly journals Effect of different growth regulators on in vitro micro-propagation of Kufri Frysona

2016 ◽  
Vol 8 (2) ◽  
pp. 535-540
Author(s):  
Priyadarshani P. Mohapatra ◽  
V.K. Batra ◽  
Subhash Kajla ◽  
Anil K. Poonia ◽  
N. Manoj Kumar
Keyword(s):  
Growth Regulators ◽  
Large Scale ◽  
Basal Medium ◽  
Potato Cultivar ◽  
Shoot Tips ◽  
Scale Production ◽  
Survival Percentage ◽  
Planting Material ◽  
Micro Propagation

In the present investigation, experiment was conducted for in vitro micro-propagation with different concentration of growth regulators in different explants Sprouts and Shoot tips of potato cultivar Kufri Frysona. The maximum survival percentage (40) of sprouts and (100%) of shoot tips were obtained when the explants were surface sterilized with 0.2% bavistin & 0.4% streptocyclin (45minutes) and 0.1% mercuric chloride (60seconds). Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The maximum shoot induction (62.5±1.44%) in 11.3±0.33 days and (74.0 ± 2.13 %) in 10.0 ± 0.50 days were reported on medium PM1 (BAP 0.25 mg/l) in sprouts and shoot tip explants respectively. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The protocol will be very useful for large-scale production of disease free planting material of potato (S. tuberosum) in future.

scholarly journals Evaluation of different approaches to buckwheat (Fagopyrum esculentum Moench.) micropropagation

2008 ◽  
Vol 44 (No. 2) ◽  
pp. 66-72 ◽  
Author(s):  
L. Klčová ◽  
M. Gubišová
Keyword(s):  
Basal Medium ◽  
Multiple Shoots ◽  
Gelling Agent ◽  
Nodal Segments ◽  
Direct Regeneration ◽  
Nodal Segment ◽  
Indolebutyric Acid ◽  
Rapid Multiplication ◽  
Fagopyrum Esculentum Moench

Plant regeneration by different techniques was evaluated in three buckwheat cultivars: indirect regeneration from cotyledons and hypocotyls, direct regeneration by nodal segment cultivation and induction of multiple shoots from seedling apices. Regenerated shoots were obtained in all procedures. The effects of BA (6-benzylaminopurine), media composition and gelling agent were tested. The regeneration efficiency of shoot apex culture was 2.65–3.33 nodal segments/explant. Cotyledon and hypocotyl segments produced 1.25–2.44 shoots per explant plated. Nodal segment cultivation yielded 4.1–4.8 new nodal segments/explant in 4 weeks. Eighty percent of shoots rooted on the basal medium. Rooting was improved (up to 95.6%) by IBA (3-indolebutyric acid) addition to the culture medium. Regenerated plantlets were transferred to the soil. The most efficient and simple micropropagation of buckwheat was nodal segment cultivation on MS medium solidified by agar with the addition of 1 mg/l BA. This method is advisable for rapid multiplication, in vitro conservation or rescue of genetic resources.

A MICROPROPAGATION TECHNIQUE OF HEMIDESMUS INDICUS (ASCLEPIADACEAE),A VALUABLE MEDICINAL PLANT

2018 ◽  
Vol 24 (1) ◽  
Author(s):  
SUSANTA KUMAR MAITY
Keyword(s):  
Medicinal Plant ◽  
Leaf Shape ◽  
Basal Medium ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Hemidesmus Indicus ◽  
Regenerated Plants ◽  
Nodular Callus ◽  
In Vitro Clonal Propagation

An efficient protocol was established for in vitro clonal propagation of Hemidesmus indicus (Anantamul) belongs to the family Asclepiadaceae, a widely used medicinal plant through callus culture in using nodal segment. Yellowish nodular callus was observed from nodal segments on MS basal medium supplemented with 0.5 mg/L BAP + 0.2 mg/L NAA within four weeks of culture. Large number of shoots (11.4±0.2) and roots (8.2±0.4) were obtained when the callus was sub cultured on MS medium with 0.2 mg/L BAP. The regenerated plantlets were acclimatized by transferring them to soil. The survival rate of plantlets was found to be 90%. Regenerated plants were morphologically comparable having normal leaf shape and growth.

scholarly journals ARTIFICIAL SEED PRODUCTION IN POINTED GOURD (TRICHOSANTHES DIOICA ROXB.)

2008 ◽  
Vol 21 (1) ◽  
pp. 15-20
Author(s):  
M. A. Malek ◽  
M. O. Islam ◽  
M. A. Bari Miah
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Conservation Strategies ◽  
Plantlet Formation ◽  
Artificial Seed ◽  
Pointed Gourd ◽  
Subsequent Regeneration

Encapsulation of nodal segments was successfully developed for pointed gourd towards the formation of artificial seed with sodium alginate. Encapsulated nodal segments (artificial seed) were cultured in MS basal medium containing different concentrations and combinations of BAP and NAA to induce germination and shoot proliferation. Highest (95%) shoot formation was obtained in MS + 1.0 mg/l BAP followed by MS + 0.5 mg/l BAP. The encapsulated nodal segments also regenerated in vitro on different substrates. Frequency of plantlet formation was low on these substrates compared to plantlet development on MS media. Among these substrates, the percentage of plantlet formation was better on moist cotton (42%) followed by filter paper (35%). The hardened plants were transferred successfully to soil in the earthen pots. The protocol for encapsulating the nodal segments for the production of artificial seeds and their subsequent regeneration is a new area of research to develop in vitro conservation strategies for pointed gourd.DOI: http://dx.doi.org/10.3329/bjpbg.v21i1.17044

scholarly journals In vitro propagation of 'Echo' cultivars of Eustoma grandiflorum (Raf.) Shinn.

2005 ◽  
Vol 11 (2) ◽  
Author(s):  
M. Ördögh ◽  
E. Jámbor-Benczúr ◽  
A. Tilly Mándy
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Basal Medium ◽  
The Other ◽  
Eustoma Grandiflorum ◽  
After Effect ◽  
Number Of Shoots

Eustoma grandiflorum (Raf.) Shinn. 'Echo' Fl cultivars ('Echo White', 'Echo Rose', 'Echo Blue', 'Echo Blue Picotee') were used and multiplication of shoots was evaluated on Murashige and Skoog (1962) basal medium with 11 g/1 agar-agar and 20 g/1 sucrose. To test the effect of BA different concentrations were added: 0.10, 0.25 mg/1 and a culture medium without BA. Differentiation of roots was examined on Jámbor-Benczúr and Marta (1990) basal medium with the same concentration of agar-agar and sucrose. To examine the effect on rooting, various concentrations of NAA were used: 0.5, 1.0, 2.0, 3.0 mg/l. The pH was adjusted to 5.6 in every case using KOH. We studied the after-effect of different concentrations of BA during the acclimatisation. During the multiplication, the cultivar 'Echo White' formed the most shoots and the smallest leaves on the medium with 0.10 mg/1 BA. Fortunately, in the case of this cultivar, the number of shoots was reduced and the length of leaves was increased succesfully on the medium without BA. The other three cultivars developed the longest leaves on the medium containing 0.10 mg/1 BA. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. Examining the rooting, the highest percent of roots was found on the medium with 1.0 mg/1 NAA, and the cultivar 'Echo Rose' formed the most roots on this medium. Higher concentration (2.0 and 3.0 mg/1) of NAA already reduced the number of roots in all of the cultivars. During the acclimatisation, the percentage of survival was 76.3% and the tallest plants with the longest leaves were found on the multiplication medium with 0.25 mg/1 BA. 'Echo Blue Picotee' gave the best results with the tallest pieces and longest leaves on this medium.

scholarly journals Plant Regeneration from Leaf Explants of the Medicinal Herb Wedelia chinensis

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 407
Author(s):  
Yung-Ting Tsai ◽  
Kin-Ying To
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Liver Toxicity ◽  
Leaf Explants ◽  
Basal Medium ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Average Percentage

Wedelia chinensis, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of W. chinensis by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA3), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of W. chinensis in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant W. chinensis.

scholarly journals Cryopreservation of Arecanut (Areca catechu L.) Embryogenic Calli by V Cryo-plate Method

2021 ◽  
pp. 76-82
Author(s):  
Kilingar Subrahmanya Muralikrishna ◽  
Kalathil Kundanchery Sajini ◽  
Pulikuthi Kavya ◽  
Krishna Prakash ◽  
Abdulla Abdulla Sabana ◽  
...  
Keyword(s):  
Crop Improvement ◽  
Basal Medium ◽  
Start Codon ◽  
Embryogenic Calli ◽  
Immature Inflorescence ◽  
Plate Technique ◽  
Plantlet Production ◽  
Scot Marker ◽  
Palm Species

Aims: Arecanut, a perennial palm species of Arecaceae family, has huge commercial value, and is grown mainly for its masticatory nuts. The ever-increasing demand for uniform quality plantlets from growers necessitates putting in place In vitro mass multiplication and other crop improvement programmes. The present study was carried out to standardize the procedure for cryopreservation of embryogenic calli of arecanut, derived from immature inflorescence cultures, by vitrification based cryo-plate technique. Study Design: Completely randomized design (CRD) with three replications. Place and Duration of Study: ICAR-Central Plantation Crops Research Institute, Kerala, India during 2019. Methodology: The embryogenic calli were precultured in Eeuwen's Y3 basal medium supplemented with sucrose (0.2, 0.3 and 0.4 M) for three days. Explants were affixed on cryo-plates and later dehydrated using plant vitrification solution 3 (PVS3) for 30 min. Cryoplates were inserted in cryovials and cryopreserved. Explants with no cryostorage served as control. Explants were rewarmed quickly in a water bath (40ºC) for 2 min and treated with unloading solution and cultured on recovery medium. Results: The results showed 8-10 % recovery of embryogenic calli that resulted in normal plantlet production. The clonal fidelity studies, using Start Codon Targeted (SCoT) marker, showed no variation of cryopreserved calli in comparison to the original calli. Conclusion: This preliminary study demonstrated the successful use of vitrification (V) cryo-plate technique in cryopreservation of embryogenic calli of arecanut. With better recovery percentage, the optimal concentration of sucrose in the preculture medium was found to be 0.3 M. Desiccation in PVS3 solution for 30 min had no adverse effect.

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