scholarly journals Determination of the In vitro Effect of Trichoderma harzianum on Phytopathogenic Strains of Fusarium oxysporum

2015 ◽  
Vol 43 (2) ◽  
Author(s):  
Hacer Handan ALTINOK ◽  
Oktay ERDOĞAN
Keyword(s):  
Fusarium Oxysporum ◽  
Trichoderma Harzianum

scholarly journals Determination of the In vitro Effect of Trichoderma harzianum on Phytopathogenic Strains of Fusarium oxysporum

2015 ◽  
Vol 43 (2) ◽  
pp. 494-500 ◽  
Author(s):  
Hacer Handan ALTINOK ◽  
Oktay ERDOGAN
Keyword(s):  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Antagonistic Effect ◽  
Significant Inhibition ◽  
Maximum Inhibition ◽  
Repressive Effect ◽  
Pre Treatment ◽  
Colonizing Ability

Fusarium oxysporum is a well-known soil-borne fungi and it is difficult to control their pathogenic strains by conventional strategies. The cultures of two strains of Trichoderma harzianum (T16 and T23) were examined in laboratory conditions and with pot experiments for the control of pathogenic strains of Fusarium oxysporum f. sp. melongenae (Fomg), Fusarium oxysporum f. sp. lycopersici (Fol), Fusarium oxysporum f. sp. niveum (Fon) and F. oxysporum f. sp. melonis (Fom). The T16 and T23 strains showed significant inhibition of mycelial growth in the pathogenic strains of F. oxysporum and the maximum inhibition were recorded when the T. harzianum strain T16 was used (72.69%). Both T. harzianum strains produced volatile and non-volatile metabolites that inhibited growth of F. oxysporum strains on PDA medium. In vitro colonization study demonstrated the root-colonizing ability of these antagonists. The interaction between T. harzianum isolates (T16 and T23) and pathogenic F. oxysporum hyphae showed no overgrowth, hyphal coiling, cell wall degradation or any hyphal penetration around any of the tested F. oxysporum hyphae. Pre-treatment of soil with T16 significantly reduced the severity of Fusarium wilt disease. The disease severity in control plants reached to 90-95% whereas those of the T16-Fomg and T16-Fol treated seedlings of eggplants were 37.74% and 47.12%, respectively, on the 21st day. In this study, while both T. harzianum isolates had a considerable antagonistic effect on the tested pathogens, T16 was found to be more successful than T23. The strong repressive effect of T. harzianum (T16) towards pathogenic Fusarium oxsporum can be applied in biological control of these pathogens.

scholarly journals MEKANISME PARASITISME TRICHODERMA HARZIANUM TERHADAP FUSARIUM OXYSPORUM PADA SEMAI ACACIA MANGIUM

2015 ◽  
Vol 15 (1) ◽  
pp. 72
Author(s):  
Susanti Tasik ◽  
Siti Muslimah Widyastuti ◽  
Harjono .
Keyword(s):  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Acacia Mangium ◽  
Culture Method ◽  
Inhibition Mechanism ◽  
Dual Culture ◽  
In Planta ◽  
Know How ◽  
Soil Borne Fungi

Mechanism of parasitism of Trichoderma harzianum on Fusarium oxysporum on Acacia mangium seedlings. Fusarium oxysporum is one of the most important soil-borne fungi the causal agent of damping-off disease. Detailed information it needed to know how the pathogen can be inhibited by Trichoderma harzianum. The objective of this research was to investigate the inhibition mechanism of T. harzianum on F. oxysporum in vitro and in planta. Green Flourescent Protein (GFP) T. harzianum was used as biocontrol agent of F. oxysporum. An in vitro inhibition test of T. harzianum was performed using dual culture method. In the in planta inhibition tests, seedlings of A. mangium were applied with GFP T. harzianum two days before inoculation of F. oxysporum; GFP T. harzianum was simultaneously applied with F. oxysporum and GFP T. harzianum was applied two days after inoculation of F. oxysporum. The inhibition effect of T. harzianum GFP was observed at seven days incubation, indicated by attachment of T. harzianum to F. oxysporum hyphae. GFP T. harzianum hyphae covered the colonies of F. oxysporum at 12 days after incubation. The highest life percentage of A. mangium seedlings was found on the treatment of GFP T. harzianum two days before inoculation of F. oxysporum (82.22%), whereas the lowest life percentage was found on seedling applied with GFP T. harzianum two days after inoculation of F. oxysporum (64.44%).

scholarly journals Antagonismo de Trichoderma harzianum contra la fusariosis del garbanzo y su efecto biofertilizante

2020 ◽  
Vol 11 (5) ◽  
pp. 1135-1147
Author(s):  
Talina Olivia Martínez-Martínez ◽  
Brenda Zulema Guerrero-Aguilar ◽  
Víctor Pecina-Quintero ◽  
Enrique González-Pérez ◽  
Juan Gabriel Angeles-Núñez
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Fusarium Solani ◽  
Baja California ◽  
Sclerotium Rolfsii ◽  
Macrophomina Phaseolina

El garbanzo es una leguminosa, que se cultiva en dos regiones de México principalmente, noroeste (Sonora, Sinaloa y Baja california) y la región de El Bajío (Guanajuato, Michoacán y Jalisco); sin embargo, cada año la producción del cultivo está comprometida con la fusariosis vascular, una de las principales enfermedades que afectan al cultivo y que está asociada al complejo fúngico Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Macrophomina phaseolina y Sclerotium rolfsii. Una alternativa de control biológico es la aplicación de Trichoderma, la que además tiene un efecto indirecto en la nutrición de la planta. El objetivo de este estudio fue determinar el antagonismo in vitro de dos cepas de Trichoderma harzianum (T1 y T2) y su efecto como biofertilizante. Se realizaron confrontaciones in vitro contra cepas de las razas de Fusariumoxysporum f. sp. ciceris (Foc 0, 1B/C, 5 y 6), Fusarium solani, Macrophomina phaseolina (MSonora y M-GTO) y Sclerotium rolfsii. Se evaluó el efecto de T2 como biofertilizante (TB) midiendo las variables: número de flores, vainas, altura de la planta, diámetro del tallo, longitud de la raíz y rendimiento de grano. Las dos cepas de T. harzianum mostraron antagonismo en diferente escala contra los patógenos. Adicionalmente, con el tratamiento donde se aplicó T. harzianum (TB) se presentaron incrementos en el número de flores (30%), vainas (24%), altura (3%), diámetro de las plantas (3.5%), así como la longitud de la raíz (13%) y rendimiento del grano (23%).

scholarly journals The biological activity of subspecies Trichoderma harzianum against Fusarium oxysporum, the causative agent of fusarium wilt cucumber in vitro

2020 ◽  
Vol 21 ◽  
pp. 00021
Author(s):  
Ninel Pavlovskaya ◽  
Irina Gneusheva ◽  
Irina Solokhina ◽  
Natalya Ageeva
Keyword(s):  
Biological Activity ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Pathogenic Fungi ◽  
Chitinase Activity ◽  
Lytic Enzymes ◽  
Growth Promoting ◽  
Phytopathogenic Microorganisms ◽  
Trichoderma Lixii

The study of the effect of the strains of the fungus Trichoderma: Trichoderma atrobrunneum VKPM F-1434, Trichoderma harzianum 5/14, Trichoderma Lixii T4/14 on the number of micromycetes populations of the pathogenic fungi Fusarium oxysporum isolate B/14, Fusarium oxysporum isolate MOS509, Fysarium oxysporum isolate IMI58289 in vitro. It was found that the Trichoderma atrobrunneum fungus strain VKPM F-1434 showed the highest degree of inhibition on 10 days of cultivation with phytopathogenic microorganisms, which was 100 %. The study revealed that all Trichoderma species are capable of producing lytic enzymes. Trichoderma atrobrunneum strain VKPM F-1434 exhibits strong lipase and chitinase activity and average proteinase activity. In addition, Trichoderma atrobrunneum strain VKPM F-1434 has a growth-promoting ability, which was reflected in the germination of seeds of cucumber “German F1”. The maximum values of indicators of germination energy were noted – 98.4 % and germination – 100 %.

scholarly journals Controle de Fusarium oxysporum f.sp. phaseoli in vitro e em sementes, e promoção do crescimento inicial do feijoeiro comum por Trichoderma harzianum

2011 ◽  
Vol 36 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Daniel D.C. Carvalho ◽  
Sueli C.M. Mello ◽  
Murillo Lobo Júnior ◽  
Mayara C. Silva
Keyword(s):  
Fusarium Oxysporum ◽  
Trichoderma Harzianum

scholarly journals Combinatorial efficacy of Trichoderma spp. and Pseudomonas fluorescens to enhance suppression of cell wall degrading enzymes produced by Fusarium wilt of Arachis hypogaea.L

2018 ◽  
Vol 7 (2) ◽  
pp. 36-42 ◽  
Author(s):  
P Rajeswari ◽  
R Kapoor
Keyword(s):  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Trichoderma Viride ◽  
Pectin Methylesterase ◽  
Biocontrol Agents ◽  
Cell Wall Degrading Enzymes ◽  
Trichoderma Spp ◽  
Pectinolytic Enzymes

Fusarium oxysporum, the soil borne pathogen causes vascular wilt, on majority of crop plants. It has been demonstrated that two different species of Trichoderma and Pseudomonas fluorescens suppress disease by different mechanisms. Therefore, application of a mixture of these biocontrol agents, and thus of several suppressive mechanisms, may represent a viable control strategy. A necessity for biocontrol by combinations of biocontrol agents can be the compatibility of the co-inoculated micro-organisms. Hence, compatibility between Trichoderma spp. and Pseudomonas fluorescens that have the ability to suppress Fusarium oxysporum in vitro on the activity of pectinolytic enzymes of Fusarium oxysporum. The activity of pectinolytic enzymes, i.e. pectin methyl esterase, endo and exo polymethylgalacturonases and exo and endo pectin trans eliminases produced by Fusarium oxysporum (Control) was higher. Maximum inhibition of pectin methylesterase, exo and endo polymethylgalacturonase and exo and endopectin trans eliminase was shown by culture filtrate of Trichoderma viride + Pseudomonas fluorescens (Tv+Pf) (1+2%), followed by Trichoderma harzianum + Pseudomonas fluorescens, (Th +Pf) (1.5+2%) and Trichoderma viride + Trichoderma harzianum (Tv+Th) (1+1.5%). However, pathogenecity suppression of Fusarium oxysporum, a causative of Arachis hypogaea. L by the compatible combination of Trichodema viride + Pseudomonas fluorescens (1+2%) was significantly better as compared to the single bio-agent. This indicates that specific interactions between biocontrol agents influence suppression of pathogenicity factors directly by combinations of these compatible bio-agents.Int. J. Agril. Res. Innov. & Tech. 7 (2): 36-42, December, 2017

scholarly journals Pengaruh Media (Campuran Beras Dan Ampas Tebu) terhadap Pertumbuhan Trichoderma harzianum dan Daya Hambatnya terhadap Fusarium oxysporum secara In vitro

BioScience ◽  
2018 ◽  
Vol 2 (1) ◽  
pp. 50
Author(s):  
Moralita Chatri
Keyword(s):  
Fusarium Oxysporum ◽  
Trichoderma Harzianum

Jamur Fusarium oxysporum merupakan jamur patogen umumnya ditemukan pada tanaman holtikultura. Jamur ini menyebabkan penyakit layu fusarium. Fungisida sintetis merupakan pilihan yang sering digunakan petani untuk menghambat penyebaran jamur ini. Namun penggunaannya menimbulkan banyak dampak negatif bagi lingkungan. Trichoderma harzianum memiliki sifat antagonis yang dapat menggantikan fungisida sintetis dalam menghambat pertumbuhan F. oxysporum. T. harzianum membutuhkan media pembawa seperti beras. Namun membutuhkan biaya yang cukup tinggi dan bersaing dengan kebutuhan manusia. Untuk itu dilakukan campuran antara beras dan ampas tebu untuk mencari media alternatif. Tujuannya agar mendapatkan media tumbuh yang lebih efektif dan efisien.Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 3 ulangan. Perlakuanya terdiri dari A. Media beras 100%, B. Media ampas tebu 100%, C. Media beras dan ampas tebu (1:1), D. Media beras dan ampas tebu (2:1), dan E. Media beras dan ampas tebu (1:2). Paremeter yang diamati yaitu pertumbuhan T. harzianum, kepadatan konidia, dan persentase hambatan terhadap  F. oxysporum.Hasil penelitian ini menunjukkan bahwa T. harzianum dapat tumbuh pada campuran media beras dengan ampas tebu. Media tumbuh mempengaruhi pertumbuhan, kepadatan konidia, serta daya hambatnya terhadap F. oxysporum Pertumbuhan yang terbaik yaitu pada media ampas tebu 100 %, kepadatan konidia tertinggi pada media beras 100% (11,6 x 109/ mL) dan persentase daya hambat tertinggi pada media ampas tebu 100% (60%). Hal ini menunjukkan bahwa media alternatif terbaik yaitu media ampas tebu 100%. Keywork : Fusarium oxysporum, layu fusarium, media, Trichoderma harzianum 

scholarly journals In vitro antagonist biocontrol of Fusarium oxysporum and Dickeya chrysanthemi

2021 ◽  
Vol 39 (3) ◽  
Author(s):  
Sarahi Rubio-Tinajero ◽  
Eduardo Osorio-Hernández ◽  
Benigno Estrada-Drouaillet ◽  
José Hugo Tomás Silva-Espinosa ◽  
Ma. De Lourdes Rodríguez-Mejía ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Aloe Vera ◽  
Trichoderma Asperellum

El objetivo fue evaluar el efecto antagónico<em> in vitro</em> de cepas nativas de <em>Trichoderma asperellum</em>, <em>Trichoderma harzianum</em> y dos aislados de <em>Bacillus</em> spp. frente a <em>Fusarium oxysporum</em> y <em>Dickeya chrysanthemi</em>. Se identificó morfológicamente a <em>Bacillus</em>, aislado de muestras de suelo de plantaciones de <em>Aloe vera</em> y se realizó una confrontación de cultivos duales modificada, colocando en el centro de la caja Petri <em>F. oxysporum</em> y en los cuatro puntos cardinales las bacterias de <em>Bacillus</em>; para esto se dispuso bajo un diseño experimental completamente al azar con cinco repeticiones, las variables registradas fueron porcentaje de antagonismo, halo de inhibición y concentración de conidios. Para <em>Trichoderma</em> se confrontaron frente a <em>F. oxysporum</em> y <em>D. chrysanthemi</em> con una confrontación dual y se determinó porcentaje de inhibición y clasificación de antagonismo. En la confrontación de <em>T. asperellum</em> obtuvo un porcentaje de inhibición de 70.5 % frente a <em>F. oxysporum</em> y 41.9 % frente a <em>D. chrysanthem</em>i. En cuanto a los halos de inhibición de <em>Bacillus</em> (B5 y B4) obtuvieron 4 mm. En la concentración de conidios B5 mostró 1.3, B4 2.6 y el testigo 12.6 esporas por dilución. <em>Trichoderma</em> y <em>Bacillus</em> representan una alternativa viable para el control de<em> F. oxysporum</em> y <em>D. chrysanthemi</em>.

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