In Vitro Organogenesis of a Slipper Orchid, Paphiopedilum  ‘Alma Gavaert’

The aim of the present study was to improve the regeneration efficiency of callus lines in a slipper orchid, Paphiopedilum ‘Alma Gavaert’. Three kinds of vegetative tissues, root, stem and leaf segments, were used as explants to induce callogenesis; out of these, only root explants formed callus and was subcultured in the presence of 5 mg/L dicamba and 5 mg/L 2,4-D combined with 1 or 2 mg/L TDZ. The resulting four callus lines, assigned as 5Di1T, 5Di2T, 5D1T and 5D2T, respectively, were used to test the effect of NAA to BA ratios on re-differentiation, wherein the highest number of shoots (approximately 2 shoots/0.1 g callus clump) were obtained in callus line 5D2T at ratios of 0.001 and 0.002. A largely improvement of shoot regeneration efficiency was obtained by continuous selection of callus lines which derived from different explant positions. Eventually, six callus lines, including 5D2T-T6-G5 to 5D2T-T6-G10, were able to produce approximately 10 times of shoots per callus clump when compared with the parental callus line 5D2T.

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In vitro selection of NaCl tolerant callus lines of Cymbopogon martinii (Roxb.) Wats

Plant Science ◽

10.1016/s0168-9452(97)04612-8 ◽

1997 ◽

Vol 124 (2) ◽

pp. 203-210 ◽

Cited By ~ 15

Author(s):

J. Patnaik ◽

B.K. Debata

Keyword(s):

In Vitro Selection ◽

Cymbopogon Martinii ◽

Callus Lines ◽

Selection Of

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Anatomical and ultrastructural analyses of in vitro organogenesis from root explants of commercial passion fruit (Passiflora edulis Sims)

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-012-0171-4 ◽

2012 ◽

Vol 111 (1) ◽

pp. 69-78 ◽

Cited By ~ 24

Author(s):

Diego Ismael Rocha ◽

Lorena Melo Vieira ◽

Francisco André Ossamu Tanaka ◽

Luzimar Campos da Silva ◽

Wagner Campos Otoni

Keyword(s):

Passion Fruit ◽

Root Explants ◽

Passiflora Edulis ◽

In Vitro Organogenesis ◽

Passiflora Edulis Sims

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Variability of rice haploids obtained in anther culture in vitro

Rossiiskaia selskokhoziaistvennaia nauka ◽

10.31857/s2500-26272019211-14 ◽

2019 ◽

pp. 11-14

Author(s):

M. V. Ilyushko ◽

M. V. Romashova

Keyword(s):

Anther Culture ◽

Plant Height ◽

Oryza Sativa L ◽

Doubled Haploids ◽

Morphological Variability ◽

Callus Line ◽

Culture In Vitro ◽

Panicle Number ◽

Callus Lines

Morphological variability of haploid plants and doubled haploids of rice obtained on one callus line in anther culture in vitro was studied. The work was carried out on rice plants Oryza sativa L. subspecies japonica Kato varietу Cascade. Regenerant plants of one callus line obtained from one rice anther (four in total) were divided into two or three groups of 20-30 plants, depending on the sample size in order of their differentiation on callus and transplantation on the rooting medium. Two callus lines (15.1 and 18.1) formed half of the haploids, half of the doubled haploids, and two other callus lines (5.1 and 7.2) numerous haploids. On callus lines with numerous haploids (5.1 and 7.2), as the regenant number increases, the size of plants decreases (plant height, number of flowers on the main panicle, number of panicles). On the lines 15.1 and 18.1 between groups of haploids and between groups of doubled haploids statistically significant differences absent. In breeding purposes for the induced doubling of the number of chromosomes in haploid regenerants with antitubulin substances such as colchicine, it is advisable to use plants that form on callus among the first. Between haploids of four callus lines and doubled haploids of two callus lines, statistically significant differences (at p=0.001) were revealed using the Hotelling's T2-criterion, calculated for the whole complex of biometric features. Haploids of different lines differed in three or four of them, doubled haploids on three of the five signs (length of panicle, productive bushiness and plant height). Varieties of interest to breeders may be improved by anther culture in vitro.

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In vitro organogenesis and efficient plant regeneration from root explants of Passiflora suberosa L. (Passifloraceae)

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-016-9747-8 ◽

2016 ◽

Vol 52 (1) ◽

pp. 64-71 ◽

Cited By ~ 7

Author(s):

Yara Brito Chaim Jardim Rosa ◽

Carolina Cassano Monte-Bello ◽

Marcelo Carnier Dornelas

Keyword(s):

Plant Regeneration ◽

Root Explants ◽

In Vitro Organogenesis

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Glyphosate selection of maize transgenic callus lines among genotypes of Ukrainian plant breeding

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v20.771 ◽

1970 ◽

pp. 237-242

Author(s):

I. O. Nitovska ◽

I. K. Komarnytskyi ◽

B. V. Morgun

Keyword(s):

Plant Breeding ◽

Transgenic Maize ◽

Pcr Analysis ◽

Agrobacterium Mediated Transformation ◽

Maize Germplasm ◽

Maize Transformation ◽

Callus Lines ◽

Selection Of ◽

Maize Callus

Aim. Glyphosate selection has a number of advantages over other commonly used selectable markers for maize. There is some natural variability within maize germplasm for degree of sensitivity to glyphosate. We investigated the selective effect of glyphosate for production transgenic maize callus after Agrobacterium-mediated transformation among geno-types of Ukrainian plant breeding. Methods. Agrobacterium-mediated transformation, glyphosate selection in vitro, and PCR analysis were used to obtain transgenic maize callus and to confirm its status. Results. An efficient selectable marker system for production transgenic maize callus lines tolerant to herbicide glyphosate was proposed. Calluses of four maze genotypes of Ukrainian plant breeding and pCB135 vector containing CP4epsps gene were used in Agrobacterium-mediated transformation experiments. Three callus maize lines of DK267×PLS61 genotype containing CP4epsps gene were obtained. Conclusions. The use of glyphosate as a selective agent after Agrobacterium-mediated transformation proved to be effective for transgenic maize callus lines production containing the gene CP4epsps. The success of Agrobacterium-mediated transformation of maize callus strongly depended on the genotype of source ma-terial. Keywords: Agrobacterium-mediated maize transformation, CP4epsps gene, glyphosate selection, PCR analysis.

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In vitro selection of winter triticale for salt resistance

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v20.773 ◽

1970 ◽

pp. 247-251

Author(s):

S. V. Pykalo ◽

O. V. Dubrovna ◽

O. A. Demydov

Keyword(s):

Salt Stress ◽

Cell Lines ◽

In Vitro Selection ◽

High Survival ◽

Winter Triticale ◽

Step Type ◽

Callus Lines ◽

Selection Of

Aim. To obtain of cell lines and plant-regenerants of winter triticale resistant for salt stress the in vitro selection was carried out. Methods. In order to select resistant to salt stress forms of triticale the efficiency of using direct and step-type in vitro selection with application of selective system based on sodium chloride has been investigated. Results. The direct and step-type in vitro selection was conducted and the selection of callus lines of triticale being resistant to simu-lated salinity was carried out. As a result, from line 38/1296 and variety Obriy respectively, 5 and 4 resistant callus lines were identified that had a high survival rate on the selective medium with 1.2 % NaCl and maintained morphogenetic potential. From the resistant lines plant regenerants were induced and their rearing, rooting and transfer to in vivo condi-tions were optimized. Conclusions. A step-type in vitro selection was more effective, because resulted from the selec-tion more resistant callus forms were identified. First cell lines of winter triticale with resistance to salt stress were de-rived.Keywords: Triticale, in vitro selection, callus, salt stress, resistance.

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