Development of Glass-Tube-Pair Type Doppler Probe Array for 1D Profile Measurement of Two Component Ion-Flow Vector

There are certain visual double stars which, when close to a node of their relative orbit, should have enough radial velocity difference (10-20 km/s) that the spectra of the two component stars will appear resolved on high-dispersion spectrograms (5 Å/mm or less) obtainable by use of modern coudé and solar spectrographs on bright stars. Both star images are then recorded simultaneously on the spectrograph slit, so that two stellar components will appear on each spectrogram.

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Exogeneous factors are not necessary in attachment, spreading and polarization of hela cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082418 ◽

1978 ◽

Vol 36 (2) ◽

pp. 310-311

Author(s):

W. Liebrich

Keyword(s):

Hela Cells ◽

Calf Serum ◽

Glass Tube ◽

Serum Free Medium ◽

Nacl Solution ◽

Free Medium ◽

Minimum Essential Medium ◽

Serum Free ◽

All Solutions ◽

Glass Support

HeLa cells were grown for 2-3 days in EAGLE'S minimum essential medium with 10% calf serum (S-MEM; Seromed, München) and then incubated for 24 hours in serum free medium (MEM). After detaching the cells with a solution of 0. 14 % EDTA and 0. 07 % trypsin (Difco, 1 : 250) they were suspended in various solutions (S-MEM = control, MEM, buffered salt solutions with or without Me++ions, 0. 9 % NaCl solution) and allowed to settle on glass tube slips (Leighton-tubes). After 5, 10, 15, 20, 25, 30, 1 45, 60 minutes 2, 3, 4, 5 hours cells were prepared for scanning electron microscopy as described by Paweletz and Schroeter. The preparations were examined in a Jeol SEM (JSM-U3) at 25 KV without tilting.The suspended spherical HeLa cells are able to adhere to the glass support in all solutions. The rate of attachment, however, is faster in solutions without serum than in the control. The latter is in agreement with the findings of other authors.

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A technique for protecting delicate specimens during processing

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100156894 ◽

1989 ◽

Vol 47 ◽

pp. 982-983

Author(s):

R.J. Mount ◽

R.V. Harrison

Keyword(s):

Basilar Membrane ◽

Low Cost ◽

Organ Of Corti ◽

Region Of Interest ◽

Sensory Epithelium ◽

Physical Damage ◽

Air Drying ◽

Specimen Handling ◽

Two Component

The sensory end organ of the ear, the organ of Corti, rests on a thin basilar membrane which lies between the bone of the central modiolus and the bony wall of the cochlea. In vivo, the organ of Corti is protected by the bony wall which totally surrounds it. In order to examine the sensory epithelium by scanning electron microscopy it is necessary to dissect away the protective bone and expose the region of interest (Fig. 1). This leaves the fragile organ of Corti susceptible to physical damage during subsequent handling. In our laboratory cochlear specimens, after dissection, are routinely prepared by the O-T- O-T-O technique, critical point dried and then lightly sputter coated with gold. This processing involves considerable specimen handling including several hours on a rotator during which the organ of Corti is at risk of being physically damaged. The following procedure uses low cost, readily available materials to hold the specimen during processing ,preventing physical damage while allowing an unhindered exchange of fluids.Following fixation, the cochlea is dehydrated to 70% ethanol then dissected under ethanol to prevent air drying. The holder is prepared by punching a hole in the flexible snap cap of a Wheaton vial with a paper hole punch. A small amount of two component epoxy putty is well mixed then pushed through the hole in the cap. The putty on the inner cap is formed into a “cup” to hold the specimen (Fig. 2), the putty on the outside is smoothed into a “button” to give good attachment even when the cap is flexed during handling (Fig. 3). The cap is submerged in the 70% ethanol, the bone at the base of the cochlea is seated into the cup and the sides of the cup squeezed with forceps to grip it (Fig.4). Several types of epoxy putty have been tried, most are either soluble in ethanol to some degree or do not set in ethanol. The only putty we find successful is “DUROtm MASTERMENDtm Epoxy Extra Strength Ribbon” (Loctite Corp., Cleveland, Ohio), this is a blue and yellow ribbon which is kneaded to form a green putty, it is available at many hardware stores.

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Electron sources: Past, present, and future

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100149659 ◽

1993 ◽

Vol 51 ◽

pp. 764-765

Author(s):

David C Joy

Keyword(s):

Glass Tube ◽

Source Size ◽

Electron Gun ◽

Operating Parameters ◽

Operational Parameters ◽

Electron Sources ◽

Difficult Decision ◽

Probe Size ◽

Wide Range ◽

Overall Performance

The electron source is the most important component of the Scanning electron microscope (SEM) since it is this which will determine the overall performance of the machine. The gun performance can be described in terms of quantities such as its brightness, its source size, its energy spread, and its stability and, depending on the chosen application, any of these factors may be the most significant one. The task of the electron gun in an SEM is, in fact, particularly difficult because of the very wide range of operational parameters that may be required e.g a variation in probe size of from a few angstroms to a few microns, and a probe current which may go from less than a pico-amp to more than a microamp. This wide range of operating parameters makes the choice of the optimum source for scanning microscopy a difficult decision.Historically, the first step up from the sealed glass tube ‘cathode ray generator’ was the simple, diode, tungsten thermionic emitter.

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Observation of the Sulphur Atoms in Cu2S Using HREM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010017894x ◽

1990 ◽

Vol 48 (1) ◽

pp. 38-39

Author(s):

Y.D. Yu ◽

R. Guan ◽

K.H. Kuo ◽

H. Hashimoto

Keyword(s):

Electron Diffraction ◽

Tube Wall ◽

Present Report ◽

Dynamic Effect ◽

Glass Tube ◽

Fluorite Structure ◽

Point Of View ◽

Electron Diffraction Analysis ◽

Sulphur Atom ◽

Copper Thin Film

We have indicated that the lighter atoms such as oxygen in Cu2O can be observed at the specimen with optimal thicknesses based on the dynamic effect of electron diffraction(1). This rule in principle should hold good for the imaging of other lighter atoms such as sulphur atom in Cu2S. However, this point of view needs further experimentally confirm because up to now only oxygen atoms have been observed in Cu2O and a series of new suboxides of copper and nickel (2). In addition, the sulphur atom is much heavier than oxygen one though is still lighter than copper atom. In the present report we provide such a confirmation.The crystallites of Cu2S shown in Fig.l were obtained by sulfurizing at 300°C of the copper thin film which was sealed in a glass tube with mg sulphur left on the tube wall in a vacuum of about 10-2 Pa. The energy dispersive spectrocscopy analysis indicated that they are the sulfides and the electron diffraction analysis indicated they have anti-fluorite structure.

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