scholarly journals Does the nitrogen application associated with Azospirillum brasilense inoculation influence corn nutrition and yield?

Author(s):  
Elton M. de Souza ◽  
Fernando S. Galindo ◽  
Marcelo C. M. Teixeira Filho ◽  
Paulo R. T. da Silva ◽  
Arthur C. dos Santos ◽  
...  

ABSTRACT The aim of this study was to investigate the synergistic effect between inoculation with Azospirillum brasilense and nitrogen application, thus enabling a higher efficiency of nitrogen fertilization, as evaluated by nutritional value, components production, and grain yield of irrigated corn. The experiment was conducted in Selvíria, MS, Brazil, under a no-till system, on an Oxisol in the Brazilian Cerrado. The experiment was set up in a randomized block design with four replications, in a 4 × 2 factorial arrangement: four patterns of nitrogen application [application of 30 kg ha-1 of N at sowing and 150 kg ha-1 as cover (30 + 150); 30 kg ha-1 of N at sowing, split into two applications of 75 kg ha-1 as cover (30 + 75 + 75); 180 kg ha-1 of N at sowing (180); and 150 kg ha-1 of N at sowing and 30 kg ha-1 as cover (150 + 30)]; with and without inoculation of the seeds with A. brasilense. The application of 30 kg ha-1 of N at sowing and a single application of 150 kg ha-1 or two applications of 75 kg ha-1 in topdressing, inoculated with Azospirillum brasilense provided better nutrition and development, with a positive reflection on irrigated corn grain yield in the Brazilian Cerrado.

2018 ◽  
Vol 10 (3) ◽  
pp. 213 ◽  
Author(s):  
Fernando S. Galindo ◽  
Marcelo C. M. Teixeira Filho ◽  
Salatiér Buzetti ◽  
Willian L. Rodrigues ◽  
Eduardo H. M. Boleta ◽  
...  

There is a lack of more comprehensive research defining how much mineral N can be applied as regards the efficiency of BNF to attain high with Azospirillum brasilense, what is a bacterium that stands out for its biological nitrogen fixation (BNF) in corn crops, reaching economically sustainable yields. Moreover, it would be interesting to investigate the use of silicon, which exerts numerous benefits on grasses, especially when the plants are subjected to biotic and abiotic stresses. The aim of this work was to study a synergistic effect between the inoculation with A. brasilense and the application of silicon, thus enabling a higher efficiency of nitrogen fertilization, evaluating the grain yield of irrigated corn in economic terms. The experiment was conducted in Selvíria, MS, Brazil, under a no-till system, on a Latossolo Vermelho distrófico (Oxisol). The experiment was set up as a randomized block design with four replications, in a 2 × 5 × 2 factorial arrangement consisting of two soil corrective sources (dolomitic limestone and Ca and Mg silicate as source of Si); five doses of N (0, 50, 100, 150 and 200 kg ha-1); with and without inoculation with A. brasilense. The inoculation makes the corn increase much more profitable, irrespective of N doses and corrective source. The N dose of 200 kg ha-1 with dolomitic limestone, associated with inoculation provided greater grain yield. However, the highest economic return was obtained at the N dose of 100 kg ha-1, with dolomitic limestone application and inoculation.


2021 ◽  
Author(s):  
Philippe Solano Toledo Silva ◽  
Ana Maria Rodrigues Cassiolato ◽  
Fernando Shintate Galindo ◽  
Arshad Jalal ◽  
Thiago Assis Rodrigues Nogueira ◽  
...  

Abstract Soil and plant beneficial microbes capitalize plant nutrition through successful colonization in roots rhizosphere which could sustainably increase cereal production. Zinc (Zn) is intensively reported a deficient nutrient for maize and wheat production in Brazilian Cerrado, which could be sustainably managed by beneficial microorganisms and their symbiotic association with other microorganisms like arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE). The objective of this study was to evaluate the effect of Azospirillum brasilense inoculation and residual Zn rates on root colonization and grain yield of maize and wheat in succession under Brazilian Cerrado conditions. These experiments were conducted in a randomized block design with four replications and arranged in a 5x2 factorial scheme. The treatments were consisted of five Zn rates (0, 2, 4, 6 and 8 kg ha-1) applied from zinc sulfate in maize and residual on wheat, and without and with seed inoculation of A. brasilense. Both crops were evaluated for root colonization of AMF and DSE, number of spores of AMF, quantification of CO2-C and grain yield. Colonization by AMF and DSE were significantly increased with interaction of Zn rates and inoculation treatments. The inoculation of A. brasilense favored root AMF and DSE colonization while increasing Zn rates by 4 kg ha-1 for maize and while 2 and 4 kg ha-1 Zn in residual for wheat reduced these colonizations. The inoculation did not influence spore production and CO2-C in both crops while maize-wheat yield were increased with Zn rates up to 4 kg ha-1 in edaphoclimatic condition of Brazilian Cerrado.


2018 ◽  
Vol 10 (4) ◽  
pp. 316
Author(s):  
Mayara M. Martins ◽  
Marcelo C. M. Teixeira Filho ◽  
Fernando S. Galindo ◽  
Salatiér Buzetti ◽  
José A. Malmonge ◽  
...  

The use of monoammonium phosphate (MAP) dissolved in organic polymers can reduce the fixation of phosphorus in the soil, and thus provide greater availability of this nutrient for long-term crops. The objective of this study was to evaluate the effect of doses of phosphorus in conventional form and incorporated MAP in biodegradable polymers, evaluating nutritional status, production components and corn grain yield, as well as the residual effect of P and N. The experiment was conducted in a no- tillage system consolidated for more than 10 years in a Latossolo Vermelho distrófico (Oxisol) with a clay texture located in Selvíria, MS, Brazil. The experimental design was a randomized block design with four replicates, arranged in a 4 × 4 factorial scheme, four forms of MAP (conventional, P1 = polymers with 30% MAP + water, P2 = polymers with 70% MAP + water and P3 = polymers with 30% of MAP), and four doses of P2O5 (0, 60, 120 and 180 kg ha-1), applied soon after emergence of the seedlings in the corn sowing line. Polymer P1 released higher amounts of P to the soil up to the R1 stage of corn, also provided the highest grain yield, indicating to be the most promising for use in Brazilian Cerrado soil.


2017 ◽  
Vol 38 (4) ◽  
pp. 1749
Author(s):  
Fernando Shintate Galindo ◽  
Marcelo Carvalho Minhoto Teixeira Filho ◽  
Maria Aparecida Anselmo Tarsitano ◽  
Salatiér Buzetti ◽  
José Mateus Kondo Santini ◽  
...  

Azospirillum brasilense is a bacterium known for its biological nitrogen fixation (BNF) in corn crops. However, there is a lack of comprehensive research defining how much mineral N should be applied to maximize the efficiency of BNF and attain high, economically sustainable yields. Moreover, it would be interesting to investigate whether adding urea with NBPT urease inhibitor might increase BNF in grasses. Therefore, the aim of this study was to determine the effect of inoculation with Azospirillum brasilense associated with N sources and doses in a Cerrado biome soil by evaluating the grain yield of irrigated corn in economic terms. The experiment was conducted in Selvíria, MS, Brazil under a no-till system on a Latossolo Vermelho distrófico (Oxisol). The experiment was set up as a randomized block design with four replications in a 2 × 5 × 2 factorial arrangement consisting of two sources of N (urea and urea with NBPT urease enzyme inhibitor) and five N doses applied as top-dressing (0, 50, 100, 150, and 200 kg ha-1), with and without the inoculation of seeds with A. brasilense. Inoculation with Azospirillum brasilense makes corn growth much more profitable, irrespective of the dose and source of N. Addition of 200 kg ha-1 N in the form of conventional urea coupled with inoculation with Azospirillum brasilense increases grain yield; however, the highest economic return is obtained with N applied at 100 kg ha-1 with conventional urea and inoculation.


2018 ◽  
Vol 3 (1) ◽  
pp. 510-523 ◽  
Author(s):  
Fernando Shintate Galindo ◽  
Marcelo Carvalho Minhoto Teixeira Filho ◽  
Salatiér Buzetti ◽  
Willian Lima Rodrigues ◽  
Guilherme Carlos Fernandes ◽  
...  

Abstract The aim of this study was to analyze whether there are differences between the inoculation with Azospirillum brasilense and the silicon application, thus enabling a higher efficiency of nitrogen fertilization, evaluating micronutrients and silicon concentration in shoots and roots of irrigated corn (Zea mays). The experiment was conducted in Selviria, Brazil, under a no-till system, on a Typic Rhodic Hapludox. The experiment was set up as a randomized block design with four replications, in a 2 × 5 × 2 factorial arrangement consisting of two soil corrective sources (dolomitic limestone and Ca and Mg silicate as source of Si); five N rates (0, 50, 100, 150 and 200 kg ha-1); with and without inoculation with A. brasilense. N rates increased B, Cu and Fe concentrations in shoots and B, Cu, Fe, Mn, Zn and Si in roots. Inoculation provided greater concentrations of B and Fe in shoots, and B in roots. Although inoculation with A. brasilense favored micronutrient uptake, it negatively affected Si concentration in shoots in 2015/16 crop. The use of Si in the form of Ca and Mg silicate promotes an increase in Mn, Si and Zn uptake in shoots and Mn and Si concentration in roots.


2020 ◽  
Vol 19 ◽  
pp. 15
Author(s):  
VINÍCIUS BALEIRO FERNANDES ◽  
FABIO LUIZ CHECCHIO MINGOTTE ◽  
ORLANDO FERREIRA MORELLO ◽  
ANDERSON PRATES COELHO ◽  
LEANDRO BORGES LEMOS

Maize inoculation with bacteria of the genus Azospirillum can reduce the consumption of nitrogen fertilizers to obtain maximum grain yield. In this context, the aim was to evaluate the effect of inoculation with Azospirillum brasilense associated with nitrogen fertilization on the maize agronomic performance. A randomized block design was used in a split-plot scheme with four replicates. Four inoculation managements with Azospirillum brasilense (T0 - control without inoculation; TC2 - seed box inoculation, 200x108 viable cells ha-1; TC3 - seed box inoculation, 400x108 viable cells ha-1; TS1 - previous inoculation of the seeds, 600x108 viable cells ha-1) associated with four top-dressing N doses (0, 60, 120 and 180 kg ha-1), applied at maize stage V6 by urea. Inoculation with Azospirillum brasilense associated with top-dressing nitrogen fertilization increases the number of grain rows per ear, number of grains per row, number of grains per ear and grain yield of maize, mainly due to its positive effects on plant nutrition. Managements with seed box inoculation with 400x108 viable cells per ha (TC3) and inoculation in seed pre-treatment (TS1) are the most recommended for maize.


2020 ◽  
Vol 5 (1) ◽  
pp. 126-137 ◽  
Author(s):  
Fernando Shintate Galindo ◽  
Marcelo Carvalho Minhoto Teixeira Filho ◽  
Salatiér Buzetti ◽  
Willian Lima Rodrigues ◽  
Guilherme Carlos Fernandes ◽  
...  

AbstractInformation regarding the interaction between biological nitrogen fixation (BNF) with Azospirillum brasilense inoculation and the use of silicon (Si) is needed. Silicon exerts numerous benefits on grasses, especially when the plants are subjected to biotic and abiotic stresses, affecting plant nutrition. The aim of this research was to determine if there is a synergistic effect between the inoculation with A. brasilense and Si use, on macro-nutrient content in corn shoot and root. The field trial was performed in Selvíria, Brazil, on a Typic Rhodic Hapludox soil under no-till system. The experimental design was a completely randomized block design with four replicates arranged in a 2 × 5 × 2 triple factorial arrangement, consisting of two soil acidity corrective sources (dolomitic limestone and Ca and Mg silicate as sources of Si); five N doses (0, 50, 100, 150 and 200 kg ha-1 applied in topdressing); with and without seed inoculation with A. brasilense. The inoculation favored N concentration in shoots and increased the N and S concentration even when associated to high N rates in topdressing. The Si as Ca and Mg silicate associated with the increment of N rates does not promote an increase in the macronutrients uptake. Although it did not favor the N use, the Si also did not negatively affect the benefits of the A. brasilense.


2021 ◽  
Vol 7 ◽  
pp. 1-12
Author(s):  
Ivan Ricardo Carvalho ◽  
Márcio Peter ◽  
Gustavo Henrique Demari ◽  
Francine Lautenchleger ◽  
Filipe Selau Carlos ◽  
...  

The objective to evaluate the maize yield components as a function of the top-dressing nitrogen partitioning in maize plants with conventional and transgenic technology. The experiment was carried out in the agricultural crops of 2012/2013 and 2013/2014, in the municipality of Tenente Portela-RS, Brazil. The experiment was set up in a randomized block design in a factorial scheme with two genetic technologies x 11 nitrogen fertilization treatments, arranged in three replications. The treatments were composed of top-dressing applications in the phenological stages V2 - two fully expanded leaves, (V2), V4 - four fully expanded leaves, (V4), V6 - six fully expanded leaves (V6) and V8 - with eight fully expanded leaves (V8) and split applications in V2+V4; V2+V6; V2+V8; V4+V6; V4+V8; V6+V8; and V2+V4+V6+V8. There was interaction between genetic technologies and levels of nitrogen fertilization in the maize crop. The highest grain yield was obtained with conventional technology because it presented plants with greater prolificacy, ear diameter and number of grains per row. Grain yield was superior with nitrogen fertilization in V4 and in nitrogen splitting in the V4 + V6, V4 + V8 and V2 + V4 + V6 + V8 stages.


Nativa ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 01 ◽  
Author(s):  
Gustavo Ribeiro Barzotto ◽  
Sebastião Ferreira Lima ◽  
Osvaldir Feliciano Santos ◽  
Gabriel Luiz Piati ◽  
Carlos Roberto Wassolowski

A cevada constitui uma importante cultura no Brasil devido, principalmente, a utilização de seu grão na produção de cervejas. O objetivo deste trabalho foi avaliar os efeitos da adubação nitrogenada e da inoculação com Azospirillum brasilense em cevada. O experimento foi instalado em campo, utilizando o delineamento em blocos casualizados em esquema fatorial, sendo quatro doses de nitrogênio (0, 40, 80 e 120 kg ha-1) na presença ou ausência de inoculação de sementes com A. brasilense e três repetições. Foram avaliadas características de crescimento e massa seca de planta, componentes de produção, teor de N e produtividade de grãos. A maior produtividade de grãos foi alcançada com 66 kg ha-1 de N, sem a aplicação da bactéria. Concluiu-se que o uso de A. brasilense aumenta a produtividade de grãos de cevada na ausência da adubação nitrogenada, mas não suplanta o ganho de produtividade apenas com o uso do N em doses superiores. A severidade de B. sorokidiana foi favorecida com a aplicação de A. brasilense nas maiores doses de N. A inoculação com A. brasilense é uma alternativa viável para aumentar a produtividade de grãos de cevada, caso se opte pelo cultivo sem fertilização mineral nitrogenada.Palavra-chave: Hordeum vulgare, bactérias diazotróficas, malte. NITROGEN FERTILIZATION AND INOCULATION WITH AZOSPIRILLUM BRASILENSE IN BARLEY ABSTRACT:The barley is an important crop in Brazil, mainly due to the use of its grain in the production of beers. The objective of this work was to evaluate the effects of nitrogen fertilization and inoculation with Azospirillum brasilense on barley. The experiment was carried out in the field, using a randomized block design in a factorial scheme, with four nitrogen doses (0, 40, 80 and 120 kg ha-1) in the presence or absence of seed inoculation with A. brasilense and three replications. Growth characteristics and dry mass of the plant, production components, N content and grain yield were evaluated. The highest grain yield was achieved with 66 kg ha-1 of N, without application of the bacteria. It was concluded that the use of A. brasilense increases the yield of barley grains in the absence of nitrogen fertilization, but does not supplant the productivity gain only with the use of N in higher doses. The B. sorokidiana severity was favored by the application of A. brasilense in the highest N doses. The inoculation with A. brasilense is a viable alternative to increase the yield of barley grains, in case of cultivation without nitrogen fertilization.Keywords: Hordeum vulgare, diazotrophic bacteria, malt. DOI:


Author(s):  
Fernando S. Galindo ◽  
Marcelo C. M. Teixeira Filho ◽  
Edson C. da Silva ◽  
Salatiér Buzetti ◽  
Guilherme C. Fernandes ◽  
...  

ABSTRACT Biological nitrogen fixation efficiency can be increased by co-inoculation with Bradyrhizobium spp. and Azospirillum brasilense, allowing even greater uptake of water and nutrients, leading to higher yields and enabling the insertion of unusual crops, such as cowpea (Vigna unguiculata L. Walp.), in the agricultural production system in the Cerrado region of Brazil. Thus, this study aimed to evaluate the technical and economic viability of cowpea in the Cerrado region, as a function of N doses and co-inoculation of seeds with Azospirillum brasilense and Bradyrhizobium. The study was carried out in a no-tillage system in Selvíria, MS, Brazil. The experimental design was a randomized block design, with four repetitions, arranged in a 3 x 5 factorial scheme, corresponding to three types of inoculation (without inoculation - control, with two strains of Bradyrhizobium spp. SEMIA 6462 and SEMIA 6463 - the conventional inoculation of cowpea, and these two strains of Bradyrhizobium spp. plus A. brasilense strains Ab-V5 and Ab-V6); and five N doses (0, 20, 40, 80 and 160 kg ha-1), as urea, applied as topdressing. The following evaluations were performed: grain yield, total operating cost, effective operating cost, gross revenue, operating profit, profitability index, equilibrium price and equilibrium yield. Co-inoculation with A. brasilense increases cowpea grain yield, which makes cowpea production in the Cerrado region of Brazil technically and economically viable, without the need to apply N fertilizers in topdressing.


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