scholarly journals Genotypic diversity of Streptococcus mutans isolated from preschoolers with and without early dental care

2019 ◽  
Vol 48 ◽  
Author(s):  
Ana Lídia Soares COTA ◽  
Janaína Accordi JUNKES ◽  
Regina Célia POLI-FREDERICO
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Dental Care ◽  
Random Amplified Polymorphic Dna ◽  
Genotypic Diversity ◽  
Caries Experience ◽  
Oral Microbiota ◽  
Preventive Program ◽  
Arbitrary Primers ◽  
History Of

Abstract Introduction Streptococcus mutans (S. mutans) exhibits extensive genotypic diversity, but the role of this variation is poorly understood. Objective To evaluate the genotypic diversity of S. mutans in preschoolers with and without early dental care and to associate it with dental caries experience. Material and method Twenty children, aged five year old, with history of dental care since they were one year old (G1) and 20 children without history of dental care (G2) were included. Their oral health status was assessed by the dmft index. S. mutans samples were isolated from the saliva and analysed by polymerase chain reaction. A total of 339 S. mutans isolates (173 from G1 and 166 from G2) were genotyped by random amplified polymorphic DNA with arbitrary primers OPA-02 and OPA-13. Result The results revealed 75 distinct genotypes of S. mutans in G1 and 73 in G2. Furthermore, G1 and G2 subjects harboured one to eight and one to seven distinct genotypes of S. mutans, respectively. A statistically significant association (P=0.021) and a moderate correlation (r= 0.503) were observed between dental caries experience and genotypic diversity of S. mutans in G1. Conclusion From the limitations of the study design, we just can hypothesize that actions of early dental care carried out by an educative-preventive program can contribute to a distinct oral microbiota.

scholarly journals Tongue Microbiota Composition and Dental Caries Experience in Primary School Children

mSphere ◽  
2021 ◽  
Vol 6 (2) ◽  
Author(s):  
Daixi Zhang ◽  
Toru Takeshita ◽  
Michiko Furuta ◽  
Shinya Kageyama ◽  
Mikari Asakawa ◽  
...  
Keyword(s):  
Dental Caries ◽  
Primary School ◽  
School Children ◽  
Dental Plaque ◽  
Primary School Children ◽  
Caries Experience ◽  
Microbiota Composition ◽  
Elderly Adults ◽  
Content Type ◽  
History Of

ABSTRACT The tongue microbiota of elderly adults is composed of two cohabiting commensal groups and their ratios are related to the number of teeth with dental caries experience. In this study, the variation in the tongue microbiota of primary school children and its relationship with the dental caries experience were investigated. We examined the tongue microbiota of 138 children aged 6 to 7 years and 11 to 12 years (61 and 77 children, respectively) who underwent annual dental examinations. The bacterial composition was determined by sequencing the V1-V2 region of the 16S rRNA gene. Cooccurrence network analysis indicated two groups of cohabiting predominant commensals in the tongue microbiota of children. The microbiota in children without a history of dental caries showed significantly higher relative abundances of one of the cohabiting groups, primarily composed of Neisseria subflava, Porphyromonas pasteri, and Fusobacterium periodonticum, compared to that in children with a history of dental caries, which is consistent with that of elderly adults with fewer teeth with dental caries experience. Linear discriminant analysis effect size (LEfSe) further identified Streptococcus oralis subsp. dentisani, belonging to the aforementioned commensal group, as a discriminant species in children without dental caries experience aged 6 to 7 years and 11 to 12 years. Our results describe the tongue microbiota composition of primary school children without history of dental caries and support the possibility that dental caries experience is accompanied by a shift in the tongue microbiota. IMPORTANCE Dental caries is now considered to be caused by acids produced by the overall dental plaque microbiota rather than by specific pathogens. This study focused on the relationship between dental caries experience and the variations in tongue microbiota, which is adjacent but separate from the dental plaque microbiota. Our results demonstrated that the tongue microbiota of primary school children with no history of dental caries experience was composed of predominant commensals with different relative abundances compared to those present in children with dental caries experience, suggesting that dental caries experience is accompanied by a shift in the tongue microbiota. The maintenance of a healthy tongue microbiota may indirectly contribute to the prevention of dental caries.

scholarly journals Evaluation of genotypic diversity of Streptococcus mutans using distinct arbitrary primers

2008 ◽  
Vol 16 (6) ◽  
pp. 403-407 ◽  
Author(s):  
Cínthia Pereira Machado Tabchoury ◽  
Maria Clara K. Sousa ◽  
Rodrigo Alex Arthur ◽  
Renata Oliveira Mattos-Graner ◽  
Altair Antoninha Del Bel Cury ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Genotypic Diversity ◽  
Arbitrary Primers

scholarly journals Correlation between the salivary Streptococcus mutans levels and dental caries experience in adult population of Chandigarh, India

2013 ◽  
Vol 07 (02) ◽  
pp. 191-195 ◽  
Author(s):  
Parampreet Pannu ◽  
Ramandeep Gambhir ◽  
Amrita Sujlana
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Adult Population ◽  
Positive Association ◽  
Caries Experience ◽  
Bacterial Titer ◽  
Males And Females ◽  
The Mean ◽  
Sample Score ◽  
Commercial Kits

ABSTRACT Objective: To determine the level of Streptococcus mutans (S. mutans) in adults of Chandigarh and to correlate the dental caries in these individuals with their S. mutans titers. Materials and Methods: Salivary levels of Streptococcus mutans, using Dentocult SM commercial kits were estimated in 200, 25-35 year old adults (males and females). Streptococcus mutans were detected in 87% of the study sample. Score 2, representing 105 - 106 CFU/ml (Colony Forming Unit) of saliva was found to be most prevalent, i.e. in 80 of 200 adults, followed by score 1, depicting S mutans with <105 CFU/ml, in 56 of 200 adults and score 3, with bacterial titer <106 CFU/ml in 38 of 200 adults. Results: Dental caries, recorded using Møller′s index, was found to be maximum in individuals with score 3, followed by score 2,1 and 0, thereby showing a positive correlation of dental caries with increasing titers of S. mutans. This correlation was statistically highly significant in males with figures as 8.73 decayed surfaces at score 2 rising to 17.38 at score 3. The mean of DMFT was higher among females than in the males in the present study. Conclusion: The split up data in males and females, showed a positive association between caries experience and salivary S. mutans scores. The results of the study will serve as a baseline data for future planning of preventive programs in adults.

Dental caries experience among Scottish secondary school children in relation to dental care

BDJ ◽  
1983 ◽  
Vol 154 (10) ◽  
pp. 327-330 ◽  
Author(s):  
A S Blinkhorn ◽  
M C Downer ◽  
C Wight
Keyword(s):  
Dental Caries ◽  
Secondary School ◽  
School Children ◽  
Dental Care ◽  
Caries Experience

scholarly journals Effects of National Dental Screening on Dental Caries Experience, Treatment, and Cost in Children

2020 ◽  
Vol 47 (3) ◽  
pp. 248-256
Author(s):  
Jonghyung Lee ◽  
Hangil Lee ◽  
Donghyun Son ◽  
Jihun Kim
Keyword(s):  
Health Insurance ◽  
Dental Caries ◽  
National Health Insurance ◽  
National Health ◽  
Dental Care ◽  
Caries Experience ◽  
Children Health ◽  
National Cohort ◽  
Dental Screening ◽  
Primary Canine

This study aimed to evaluate the effects of national dental screening for children in Korea in the aspects of prevention of dental caries, the number of treatments related to dental caries, and cost of dental care.The study used the national cohort data on children health screening provided by National Health Insurance Service, and analyzed 32,937 1st dental screening, 22,608 2nd dental screening, 13,708 3rd dental screening, and 241,043 cases from the dental care of 27,944 children born in year 2008 and 2009. The decayed-filled teeth index and decayed-filled person rate, calculated from the results of the 2nd and 3rd dental screening, decreased as the number of preceding dental screening increased. Glass ionomer and amalgam restoration, pulp treatment of primary teeth and extraction of primary canine and molar were shown to decrease as the number of examined dental screening increased, and the total cost of dental care covered by national health insurance also decreased as the number of dental screening increased.In conclusion, national dental screening for children is an efficient and economical oral health care method that prevents dental caries and lowers the possibility of dental caries related treatment, thereby reducing cost of dental care.

scholarly journals Interdental Localization of Streptococcus mutans as Related to Dental Caries Experience

1974 ◽  
Vol 9 (3) ◽  
pp. 481-488 ◽  
Author(s):  
R. J. Gibbons ◽  
P. F. Depaola ◽  
D. M. Spinell ◽  
Z. Skobe
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Caries Experience

scholarly journals QPCR Screening of Pediatric Saliva Samples to Evaluate Effects of Dental Sealants on Cariogenic Bacteria Streptococcus Mutans and Scardovia Wiggsiae

2021 ◽  
Vol 2 (1) ◽  
pp. 14-20
Author(s):  
Olivia Tsang ◽  
Kevin Major ◽  
Sonia Santoyo ◽  
Karl Kingsley ◽  
Linh Nguyen
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Bacterial Species ◽  
Conclusive Evidence ◽  
Oral Microbiota ◽  
Average Amount ◽  
Fluoride Treatment ◽  
Dental Sealants ◽  
Exact Effect ◽  
The Impact

Dental caries is an alarmingly prevalent multifactorial disease worldwide but is most common as a chronic disease among children and adolescents. Within the oral cavity there are hundreds bacterial species, including specific cariogenic pathogens such as Streptococcus mutans and Scardoviawiggsiae. Although dental caries are highly prevalent, it may also be preventable using methods such as dental sealants or fluoride treatment, which have proven to be highly effective in reducing caries risk – although there is limited evidence on the exact effect of these treatments on S. wiggsiaeinparticular. Due to the lack of conclusive evidence, this study is aimed to evaluate the microbial burden of S. mutans and S. wiggsiae among pediatric saliva samples to analyze the effects dental sealants have on these oral microbiota. Saliva samples derived from pediatric patients pre- and post-sealant were obtained from an existing repository. DNA was extracted and screened using qPCR and primers specific for the cariogenic organisms, S. mutans (SM) and S. wiggsiae (SW). These data demonstrated an overall reduction in SW among Post-sealant samples (11.14 U/uL) compared with Pre-sealant samples (11.68 U/uL), as well as a reduction in the overall number of samples harboring S. wiggsiae (n=8). The Pre-sealant average amount of SM (14.54 U/uL) was slightly increased among the Post-sealant samples (15.67 U/uL) with half of the samples analyzed (n=13) exhibiting slight increases in SM levels, and nearly one-third (n=8) exhibiting slight decreases in SM levels. The data from this study may suggest that dental sealants have an impact on certain cariogenic pathogens in the oral microbiota. Whether or not the impact is positive or not in light of the increase in S. mutans remains an observation that should be further analyzed. More research is required to address the limitations found in this study in order to localize the exact effect on the teeth with dental sealants.

In vivo Relationships of the Dextran-Degrading Oral Microbiota to Streptococcus mutans and Caries Experience

1982 ◽  
Vol 16 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Robert H. Staat ◽  
Sharon D. Langley ◽  
James I. Swenson
Keyword(s):  
Streptococcus Mutans ◽  
Caries Experience ◽  
Oral Microbiota
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