scholarly journals PHYTOCHEMICAL AND ANTIOXIDANT COMPOSITION IN ACCESSIONS OF THE MANGABA ACTIVE GERMPLASM BANK

2021 ◽  
Vol 34 (1) ◽  
pp. 228-235
Author(s):  
PRISCILLA SANTANA SANTOS ◽  
LISIANE DOS SANTOS FREITAS ◽  
EVANDRO NEVES MUNIZ ◽  
JOSEFA GRASIELA SILVA SANTANA ◽  
ANA VERUSKA CRUZ DA SILVA
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Antioxidant Properties ◽  
Principal Component ◽  
Total Phenols ◽  
Germplasm Bank ◽  
Fruit Species ◽  
Hancornia Speciosa

ABSTRACT Mangaba (Hancornia speciosa Gomes) is a fruit species native to Brazil, belonging to the Apocynaceae family. Its cultivation is predominantly extractivist and its antioxidant properties have been recently reported. The objective of this study was to evaluate the bioactive compounds and antioxidant activity in fruits of accession from the Active Germplasm Bank of Embrapa Coastal Tablelands (BI, CA, LG, PR, PT and TC). The extracts were analyzed by high-performance liquid chromatography (HPLC), which identified chlorogenic acid (93.71 - 131.66 mg.100g-1), ferulic acid (0.85 - 2.27 mg.100g-1) and rutin (238.59 -442.94 mg.100g-1). The accessions CA and PR showed the highest values of total phenols (1179.39 and 1167.05 mg GAE.100g-1, respectively). The accessions CA and TC had the highest concentration of the major compound rutin (436.78 and 442.94 mg.100g-1, respectively). Antioxidant activity values ranged from 125.95 to 158.67 g.g-1 DPPH. Principal component analysis grouped the accessions into four groups, due to the genetic variability verified in previous studies. The results will be useful to guide actions of selection and future breeding program of the species.

Denaturing high-performance liquid chromatography and principal component analysis for identification of DNA point mutations in breast cancer and lymphoma samples

2018 ◽  
Vol 32 (10) ◽  
pp. e3053
Author(s):  
Yocanxóchitl Perfecto-Avalos ◽  
Raquel Cuevas-Díaz Durán ◽  
Luis Villela ◽  
Alejandro Garcia-Gonzalez ◽  
Ricardo Javier Díaz-Domínguez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Principal Component Analysis ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Point Mutations ◽  
Principal Component ◽  
Component Analysis

scholarly journals Comparison of Phenolic Compounds and the Antioxidant Activities of Fifteen Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’ in China

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 325 ◽  
Author(s):  
Jinyan Gong ◽  
Bingquan Chu ◽  
Lingxiao Gong ◽  
Zhongxiang Fang ◽  
Xiaoxu Zhang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
High Performance Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Chrysanthemum Morifolium ◽  
Caffeoylquinic Acids ◽  
Caffeoylquinic Acid ◽  
Chrysanthemum Morifolium Ramat

This study investigated the phenolic compounds of 15 Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’, including 6 ‘Duoju’ and 9 ‘Taiju’, using high performance liquid chromatography (HPLC). The antioxidant activities of these ‘Hangbaiju’ were estimated by DPPH, ABTS and FRAP assays. Results show that a total of 14 phenolic compounds were detected in these flowers, including 3 mono-caffeoylquinic acids, 3 di-caffeoylquinic acids, 1 phenolic acid and 7 flavonoids. ‘Duoju’ and ‘Taiju’ possess different concentrations of phenolic compounds, and ‘Taiju’ exhibits higher caffeoylquinic acids and stronger antioxidant activities than ‘Duoju’. Caffeoylquinic acids show a strong correlation with the antioxidant activities of the samples. Principal component analysis (PCA) reveals an obvious separation between ‘Duoju’ and ‘Taiju’, using phenolic compounds as variables. Apigenin-7-O-glucoside, 3,5-di-O-caffeoylquinic acid, luteolin and acacetin were found to be the key phenolic compounds to differentiate ‘Duoju’ from ‘Taiju’.

Establishment and comparative analysis of HPLC fingerprints of deer tissues

2020 ◽  
Vol 60 (10) ◽  
pp. 1343
Author(s):  
Mengjie Yao ◽  
Haiping Zhao ◽  
Xiaoyan Qi ◽  
Yuan Xu ◽  
Wenyuan Liu ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Evaluation System ◽  
High Performance ◽  
Principal Component ◽  
Component Analysis ◽  
Chemometric Methods

Context With the increasing use of velvet antlers (VA) as functional food or traditional Chinese medicine, the quality control has become more and more important. Aims Establish an effective method to provide a way of distinguishing VA from other types of deer tissue. Methods In the present study, 18 samples from three types of deer tissue were analysed on the basis of high-performance liquid chromatography, and a chromatogram of each sample was obtained. Then, these chromatograms were processed using the similarity evaluation system for chromatographic fingerprints of traditional Chinese medicine, to give the fingerprints of three deer tissues. The chemometric methods were used to analyse the fingerprint results, so as to identify the three types of deer tissue. Key results Shared peaks of VA, venison and deer bone were identified using similarity evaluation system. The results showed that, in total, 19 peaks were identified among these three types of deer tissue. Compared with venison, VA lacked three peaks (Numbers 3, 4 and 17); compared with deer bone, VA had six extra peaks (Numbers 2, 5, 8, 9, 14 and 19). The results of chemometric methods showed that different tissue samples could be classified into three categories by using both cluster analysis and principal component analysis. After principal component analysis and partial least-square discrimination analysis, seven peaks were selected, which had significant influence on the classification of VA, venison and deer bone. Conclusions The high-performance liquid-chromatography fingerprints in combination with chemometric methods can be used to effectively distinguish three deer tissue types, namely, VA, venison and deer bone. Implications We believe the method offers a useful tool much needed in the current Chinese velvet market.

scholarly journals Comparison of Phenolic Compounds and Antioxidant Activities of Fifteen Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’ in China

2019 ◽  
Author(s):  
Jinyan Gong ◽  
Bingquan Chu ◽  
Lingxiao Gong ◽  
Zhongxiang Fang ◽  
Xiaoxu Zhang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
High Performance Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Chrysanthemum Morifolium ◽  
Caffeoylquinic Acids ◽  
Caffeoylquinic Acid ◽  
Chrysanthemum Morifolium Ramat

This study investigated the phenolic compounds of 15 Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’, including 6 ‘Duoju’ and 9 ‘Taiju’ using high performance liquid chromatography. The antioxidant activities of these ‘Hangbaiju’ were estimated by DPPH, ABTS and FPAR assays. Results showed that a total of 14 phenolic compounds were detected in these flowers, including 3 mono-caffeoylquinic acids, 3 di-caffeoylquinic acids, 1 phenolic acid and 7 flavonoids. ‘Duoju’ and ‘Taiju’ possessed different concentration of phenolic compounds, and ‘Taiju’ exhibited higher caffeoylquinic acids and stronger antioxidant activities than ‘Duoju’. Caffeoylquinic acids showed a strong correlation with the antioxidant activities of the samples. Principal component analysis revealed an obvious separation between ‘Duoju’ and ‘Taiju’ using phenolic compounds as variables. Apigenin-7-O-glucoside, 3,5-di-O-caffeoylquinic acid, luteolin and acacetin were found to be the key phenolic compounds to differentiate ‘Duoju’ from ‘Taiju’.

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