PREVALENCE OF GENETIC SUSCEPTIBILITY FOR CELIAC DISEASE IN BLOOD DONORS IN SÃO PAULO, BRAZIL

ABSTRACT Background Celiac disease is a permanent intolerance induced by gluten, which is expressed by T-cell mediated enteropathy, and has a high prevalence in the general population. There is evidence of a strong genetic predisposition to celiac disease. Objective To determine the prevalence of genetic markers HLA-DQ2 and HLA-DQ8 in blood donors from São Paulo and measure human recombinant tissue transglutaminase antibody IgA class in HLA-DQ2 and HLA-DQ8 positive donors. Methods A total of 404 blood donors from São Paulo city and Jundiaí were included in the study and signed the informed consent form. Information regarding diarrhea, constipation and abdominal pain in the last 3 months was collected. Determination of HLADQ2 and HLADQ8 alleles was performed in all participants and human recombinant tissue transglutaminase antibody class IgA was measured only in blood donors who presentedDQ2 and/or DQ8. Results HLADQ2 and/or HLADQ8 were positive in 49% (198/404) of subjects. Positive samples were associated with alleles DR3, DR4, DR7, DR11 and DR12. The most frequent genotype was DR4-DQ8, which was present in 13.6% of samples, followed by genotypes DR3-DQ2 and DR7-DQ2 with DQB1*02 in heterozygous, which were present in 10.4% and 8.7%, respectively. Eleven out of 198 positive donors (5%) were positive to human tissue transglutaminase test. Conclusion We observed a high prevalence of genetic markers for celiac disease, HLA-DQ2 and HLA-DQ8, in blood donors from São Paulo, similar to prevalence described in Europe. These findings show that the prevalence of celiac disease should not be rare in our country, but underdiagnosed.

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Prevalence of celiac disease among blood donors in SÃO PAULO – the most populated city in Brazil

Clinics ◽

10.6061/clinics/2012(09)05 ◽

2012 ◽

Vol 67 (9) ◽

pp. 1013-1018 ◽

Cited By ~ 17

Author(s):

ML Alencar ◽

CL Ortiz-Agostinho ◽

I Nishitokukado ◽

AO Damiao ◽

CP Abrantes-Lemos ◽

...

Keyword(s):

Celiac Disease ◽

Blood Donors ◽

Sao Paulo ◽

São Paulo

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High prevalence of hepatitis E virus antibodies in Sao Paulo, Southeastern Brazil: analysis of a group of blood donors representative of the general population

The Brazilian Journal of Infectious Diseases ◽

10.1016/j.bjid.2017.05.004 ◽

2017 ◽

Vol 21 (5) ◽

pp. 535-539 ◽

Cited By ~ 13

Author(s):

Ana Maria Passos-Castilho ◽

Mônica Renata Reinaldo ◽

Anne de Sena ◽

Celso F.H. Granato

Keyword(s):

General Population ◽

Hepatitis E Virus ◽

Blood Donors ◽

Hepatitis E ◽

Sao Paulo ◽

Southeastern Brazil ◽

São Paulo ◽

High Prevalence

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Prevalence and Demographic Characteristics of Celiac Disease Among Blood Donors in Ribeirão Preto, State of São Paulo, Brazil

Digestive Diseases and Sciences ◽

10.1007/s10620-006-9340-9 ◽

2006 ◽

Vol 51 (5) ◽

pp. 1020-1025 ◽

Cited By ~ 36

Author(s):

Suzeidi Bernardo Castanheira Melo ◽

Maria Inez Machado Fernandes ◽

Luiz Cesar Peres ◽

Luiz Ernesto Almeida Troncon ◽

Livia Carvalho Galvão

Keyword(s):

Celiac Disease ◽

Blood Donors ◽

Sao Paulo ◽

Demographic Characteristics ◽

São Paulo

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STANDARDIZED CLINICAL PATHWAYS FOR ESOPHAGECTOMY ARE NOT A REALITY IN BRAZIL, EVEN WITH A HIGH PREVALENCE OF ESOPHAGEAL CANCER AND ACHALASIA

ABCD Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) ◽

10.1590/s0102-67202015000300011 ◽

2015 ◽

Vol 28 (3) ◽

pp. 190-192 ◽

Cited By ~ 1

Author(s):

Marina ZAMUNER ◽

Fernando A. M. HERBELLA ◽

José L. B. AQUINO

Keyword(s):

Clinical Pathways ◽

Early Mobilization ◽

Sao Paulo ◽

Work Team ◽

Multidisciplinary Teams ◽

São Paulo ◽

Clinical Practices ◽

Paulo State ◽

High Prevalence ◽

São Paulo State

Background: The adoption of standardized protocols and specialized multidisciplinary teams for esophagectomy involve changes in routines with the implantation of expensive clinical practices and deviations from ingrained treatment philosophies. Aim: To evaluate the prevalence of standardized protocols and specialized multidisciplinary teams in São Paulo state, Brazil. Methods: Institutions that routinely perform esophagectomies in São Paulo were contacted and questioned about the work team involved in the procedure and the presence of standardized routines in the preoperatory care. Results: Fifteen centers answered the questionnaire: 10 (67%) public institutions and five (33%) private. There were seven (47%) medical schools, six (40%) with a residency program and two (13%) nonacademic institutions. The mean number of esophagectomies per year was 23. There was a multidisciplinary pre-operative team in nine (60%). There was a multidisciplinary postoperative team in 11 (73%). Early mobilization protocol was adopted in 12 (80%) institutions, early feeding in 13 (87%), routinely epidural in seven (47%), analgesia protocol in seven (47%), hydric restriction in six (40%), early extubation in six (40%), standardized hospitalization time in four (27%) and standardized intensive care time in two (13%). Conclusion: The prevalence of standardized protocols and specialized teams is very low in Sao Paulo state, Brazil. The presence of specialized surgeons is a reality and standardized protocols related directly to surgeons have higher frequency than those related to other professionals in the multidisciplinary team.

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SEROPREVALENCE OF CHAGASIC INFECTION IN YOUNG INDIVIDUALS IN A BLOOD CENTER IN THE STATE OF SAO PAULO, BRAZIL

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652013000400005 ◽

2013 ◽

Vol 55 (4) ◽

pp. 245-250 ◽

Cited By ~ 3

Author(s):

Elaine Cristina Navarro ◽

Renata Leme Goto ◽

Isabella Silva Ricoboni ◽

Jose Eduardo Corrente ◽

Rita Maria Saccomano Henriques ◽

...

Keyword(s):

Public Health ◽

Chagas Disease ◽

Blood Donors ◽

Public Health Problem ◽

Sao Paulo ◽

São Paulo ◽

Blood Center ◽

Negative Results ◽

Central Regions ◽

The City

SUMMARY This study aimed at estimating the number of cases of non-negative serological reactions to Chagas disease in blood donors at the Blood Center of Botucatu, São Paulo, Brazil, from 2003 to 2010 and at relating them to their cities of origin. Five hundred and seventy-four non-negative results for Chagas disease were evaluated. Of these, 371 (64.8%) were reagent, and 203 (35.4%) were inconclusive. The prevalence of Chagas disease in blood donors was 0.05%. There were, on average, 72 cases/year, and a prevalence of males was observed (64.8%). Forty-three (7.49%) individuals were 18 to 30 years old; 92 (16.02%) were 31 to 40; 147 (25.61%) 41 to 50, and 292 (50.87%) were older than 50 years. It was observed that 29.3% of females with reagent serology were at their fertile age (18 and 45 years). The majority of donors were originally from cities in the southwestern and central regions of São Paulo, but individuals from other states contributed with 20%. The provenance of most donors was the city of Botucatu/SP, followed by the city of Taquarituba/SP. Therefore, the profile of donors at this blood center favors the occurrence of a larger number of non-negative serological reactions. Although there has been a significant reduction in the number of new cases/year for this disease, it is still a public-health problem, and results suggest the need for new epidemiological assessments in the studied region.

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The Prevalence of Celiac Disease-Specific Auto-Antibodies in Type 1 Diabetes in a Moroccan Population

International Journal of Endocrinology ◽

10.1155/2019/7895207 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9

Author(s):

Ider Oujamaa ◽

Majda Sebbani ◽

Lahcen Elmoumou ◽

Aïcha Bourrahouate ◽

Rabiy El Qadiry ◽

...

Keyword(s):

Celiac Disease ◽

Tissue Transglutaminase ◽

Iga Deficiency ◽

Cross Sectional Study ◽

Biological Data ◽

Cross Sectional ◽

Systematic Screening ◽

Lower Height ◽

Hla Dq2

Objective. We aimed to determine the prevalence of specific auto-antibodies to celiac disease (CD) in Moroccan type 1 diabetic (T1D) patients and compare the clinical and biological characteristics of seropositive and seronegative cases. Patients and Methods. A cross-sectional study was carried out on 276 T1D patients including 109 adults and 167 pediatric cases. The screening for CD was performed by an Elisa IgA anti-tissue transglutaminase antibody (tTGA) testing, combined with IgA quantification by nephelometry. Positive-IgA-tTGA cases were secondly tested for anti-endomysial antibodies (EMA) using an immunofluorescence technique, and the IgA deficiency cases were screened for IgG-tTGA. Patients with low positive tTGA titers underwent HLA-DQ2/DQ8 typing. Sociodemographic and clinical data of the patients were collected using a hetero-administered questionnaire. The comparison of clinical and biological data between seropositive and seronegative diabetics was done using independent T, Mann–Whitney U, chi-squared, and Fisher tests, which were considered significant if p value <0.05. Results. The prevalence of CD-specific auto-antibodies was estimated to be 9.1% (IC = 95%), with 25 positive cases in tTGA and EMA testing. Eight cases displayed low titers of IgA-tTGA, among which 4 were positive for HLA-DQ2, 1 for HLA-DQ8, and 1 for both DQ2 and DQ8. The other 2 cases had a biopsy-proven CD. Compared to seronegative patients, seropositive cases had a higher percentage of associated autoimmune disorders (16% vs. 2.4%, p=0.008), with a significant lower height Z-scores (median: −0.90 (−3.93 to 0.95) vs. −0.51 (−4.54 to 2.18), p=0.029) and a higher HbA1c level (median: 11.30% (7.31 to 16.00) vs. 9.30% (4.40 to17.31), p=0.022). Conclusion. The current study gave evidence of a high prevalence of CD specific auto-antibodies in T1D population. The co-existence of these two conditions was associated with a poor glycemic control, a lower height, and other autoimmune diseases. These findings may suggest the necessity of a systematic screening of CD in T1D patients.

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Coinfection by Strongyloides stercoralis in blood donors infected with human T-cell leukemia/lymphoma virus type 1 in São Paulo city, Brazil

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762000000500017 ◽

2000 ◽

Vol 95 (5) ◽

pp. 711-712 ◽

Cited By ~ 21

Author(s):

Pedro P Chieffi ◽

Carlos S Chiattone ◽

Elder N Feltrim ◽

Rita CS Alves ◽

Maria A Paschoalotti

Keyword(s):

T Cell ◽

Virus Type ◽

Blood Donors ◽

Strongyloides Stercoralis ◽

Sao Paulo ◽

São Paulo ◽

T Cell Leukemia ◽

Cell Leukemia ◽

Human T Cell

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Zika virus seroprevalence in blood donors from the Northeastern region of São Paulo State, Brazil, between 2015 and 2017

Journal of Infection ◽

10.1016/j.jinf.2019.10.002 ◽

2020 ◽

Vol 80 (1) ◽

pp. 111-115 ◽

Cited By ~ 1

Author(s):

Svetoslav Nanev Slavov ◽

Rafael Rahal Guaragna Machado ◽

Alexander Rodrigo Ferreira ◽

Camila Pereira Soares ◽

Danielle Bastos Araujo ◽

...

Keyword(s):

Zika Virus ◽

Blood Donors ◽

Sao Paulo ◽

São Paulo ◽

Northeastern Region ◽

Paulo State ◽

São Paulo State

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High prevalence of clindamycin resistance in Staphylococcus aureus blood culture isolates in São Paulo, Brazil

Journal of Laboratory Physicians ◽

10.4103/jlp.jlp_161_16 ◽

2017 ◽

Vol 9 (04) ◽

pp. 314-316 ◽

Cited By ~ 1

Author(s):

Felipe S. Lupinacci ◽

Daniel Bussius ◽

Felipe Acquesta ◽

Gustavo Fam ◽

Raphael Rossi ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Blood Culture ◽

Susceptibility Testing ◽

Sao Paulo ◽

Resistance Rate ◽

Blood Cultures ◽

São Paulo ◽

Inducible Clindamycin Resistance ◽

Total Prevalence ◽

High Prevalence

Abstract BACKGROUND: Clindamycin has become an important antimicrobial option for the treatment of Staphylococcus aureus. However, little is known about the current patterns of clindamycin-susceptibility in S. aureus invasive isolates, both in our country and in other developing countries in the world. AIMS: The aim of this study was to determine the prevalence of constitutive and inducible clindamycin resistance in methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) blood culture isolates in São Paulo, Brazil. MATERIALS AND METHODS: From July 2011 to June 2012, all S. aureus isolates from blood cultures collected at our hospital were included in the study. Antimicrobial susceptibility testing was performed according to recommendations of the Clinical and Laboratory Standards Institute. RESULTS: Total prevalence of clindamycin resistance was 68%, including 7.2% with inducible resistance. In MRSA resistance rate was 90.8% whereas in MSSA the rate was 32.7%. CONCLUSIONS: Our high prevalence of clindamycin resistance highlights the importance of performing D-test in a routine base, as well of maintaining continued surveillance for the prevalence of clindamycin resistance.

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