scholarly journals Occurrence of Mycoplasma haemocanis in dogs infested by ticks in Campo Grande, Mato Grosso do Sul, Brazil

2016 ◽  
Vol 25 (3) ◽  
pp. 360-363 ◽  
Author(s):  
Rodrigo Leite Soares ◽  
Jessica Teles Echeverria ◽  
Giovana Pazzuti ◽  
Herbert Patric Kellerman Cleveland ◽  
Verônica Jorge Babo-Terra ◽  
...  
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Epidemiological Factors ◽  
Epidemiological Features ◽  
Blood Sucking ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Dog Blood ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Abstract Hemotropic mycoplasmas in dogs, such as Mycoplasma haemocanis, have been described worldwide. Recently, these pathogens have been reported to be causative agent of zoonosis. It is known that its transmission may occur through the action of blood-sucking arthropods (e.g. ticks or fleas), through blood transfusion, contaminated fomites and/or transplacentally. In Brazil, M. haemocanis is present in practically all regions and the tick Rhipicephalus sanguineus sensu lato is suspected the main vector. In the municipality of Campo Grande, state of Mato Grosso do Sul, there is little information about infection of dogs by M. haemocanis, or on the main epidemiological features associated with it. Thus, the aim of the present study was to determine the occurrence of M. haemocanis among dogs infested by ticks and to assess possible associations with some epidemiological factors. The polymerase chain reaction (PCR) and DNA sequencing were used to analyze dog blood samples (n = 94). DNA from M. haemocanis was detected in four samples. No significant associations were observed with any epidemiological parameter analyzed here. However, the results from this study confirm that this pathogen is circulating in this region and should be considered in the differential diagnosis of diseases among anemic dogs.

scholarly journals Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

2019 ◽  
Vol 28 (1) ◽  
pp. 80-90 ◽  
Author(s):  
Jamille Batista Faria Prado ◽  
Carlos Alberto do Nascimento Ramos ◽  
Vagner Ricardo da Silva Fiuza ◽  
Veronica Jorge Babo Terra
Keyword(s):  
Enterocytozoon Bieneusi ◽  
Domestic Cat ◽  
Domestic Cats ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Source Of Infection ◽  
Two Samples ◽  
Intestinal Pathogen ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Abstract Enterocytozoon bieneusi is an opportunistic intestinal pathogen that infects humans and a wide variety of animals worldwide. Our aim in this study was to investigate the occurrence of E. bieneusi in a domestic cat population in Campo Grande, Mato Grosso do Sul, Brazil. Sixty fecal samples from diarrheic cats were subjected to polymerase chain reaction (PCR) and the amplicons were sequenced for identification. E. bieneusi was detected in two samples (3.3%), both identified as genotype D. This genotype has already been reported in animals and humans and is considered a zoonotic genotype. Our findings represent the first report of E. bieneusi in domestic cats in Brazil, reinforcing the importance of identifying this agent as a source of infection in animals and humans.

scholarly journals Identification of class 1 and 2 integrons from clinical and environmental Salmonella isolates

2014 ◽  
Vol 8 (12) ◽  
pp. 1518-1524 ◽  
Author(s):  
Fábio Ederson Lopes Corrêa ◽  
Fabiana Gomes da Silva Dantas ◽  
Alexeia Barufatti Grisolia ◽  
Bruno do Amaral Crispim ◽  
Kelly Mari Pires Oliveira
Keyword(s):  
Chain Reaction ◽  
Mato Grosso ◽  
Future Studies ◽  
Minimum Inhibitory Concentrations ◽  
Class 1 ◽  
Resistant Strains ◽  
Polymerase Chain ◽  
Different Sources ◽  
Mato Grosso Do Sul

Introduction: The indiscriminate use of antimicrobials has selected for the emergence of resistant strains. Many mechanisms contribute to the spread of antimicrobial-resistant genes, and integrons play a key role in this process. The aim of this study was to describe the serotypes and resistance profiles, and to characterize the presence of integrons in Salmonella strains isolated from Dourados, Mato Grosso do Sul, Brazil. Methodology: Thirty-six isolates from different sources were used. To evaluate the resistance profiles, the determination of minimum inhibitory concentrations together with polymerase chain reaction were used to screen for the presence of class 1 and class 2 integrons. Results: The Infantis serotype of Salmonella was the most frequently isolated serotype. Minimum inhibitory concentrations showed that out of the 36 isolates, 11 (30.5%) were resistant to all the antimicrobials tested. These resistant isolates were separated into three groups: 4 clinical isolates (36.4%), 3 food isolates (36.4%), and 4 water isolates (27.2%). Class 1 integrons occurred in 31 (86.1%) isolates and were found in all 11 resistant isolates (35.5 %) and in 20 (64.5%) of the non-resistant isolates. Class 2 integrons were found in 3 (8.3%) isolates, which were all non-resistant. Conclusion: The presence of an integron did not necessarily confer resistance. Future studies will seek to identify the mechanism behind integron-mediated antimicrobial resistance.

scholarly journals HUMAN PARASITISM BY Rhipicephalus sanguineus sensu lato (ACARI: IXODIDAE) IN MATO GROSSO DO SUL, WEST-CENTRAL BRAZIL

2017 ◽  
Vol 54 (1) ◽  
pp. 96 ◽  
Author(s):  
Igor Cunha Lima Acosta ◽  
Thiago Fernandes Martins ◽  
Marcelo Bahia Labruna
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Mato Grosso ◽  
Central Brazil ◽  
West Central ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Mato Grosso Do Sul

O parasitismo humano pelo carrapato marrom do cão, Rhipicephalus sanguineus sensu lato (s. l.), um importante parasita para a saúde pública e veterinária, é raramente relatado no continente americano. Este trabalho relata o registro de um macho de R. sanguineus s. l. parasitando um humano na cidade de Campo Grande, estado do Mato Grosso do Sul, no Centro-Oeste do Brasil. Essa observação é relevante para a saúde pública, uma vez que os carrapatos desse complexo são conhecidos como vetores de riquétsias do grupo da febre maculosa para cães e humanos.

scholarly journals SIMULTANEOUS INFECTION WITH DENGUE 1 AND 2 IN A BRAZILIAN PATIENT

1998 ◽  
Vol 40 (3) ◽  
pp. 151-154 ◽  
Author(s):  
Iray Maria ROCCO ◽  
Maria Luisa BARBOSA ◽  
Emilia Hiromi Nakaya KANOMATA
Keyword(s):  
Virus Isolation ◽  
Western Region ◽  
Double Infection ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Brazilian Patient ◽  
Dengue Outbreaks ◽  
Simultaneous Infection ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Dengue outbreaks have occurred in several Brazilian States since 1986 involving serotypes 1 (DEN-1) and 2 (DEN-2). In view of the few cases of double infection documented in the literature, we report here a case of simultaneous infection with DEN-1 and DEN-2 in a patient residing in the municipality of Miranda, State of Mato Grosso do Sul, Western region of Brazil. DEN-1 was introduced in this State in 1989 and DEN-2 in 1996, both of them circulating in some municipalities. This double infection was identified by virus isolation and by indirect immunofluorescence using monoclonal antibodies and confirmed by the polymerase chain reaction (PCR). This is the first documented case of simultaneous infection with serotypes DEN-1 and DEN-2 in Brazil.

scholarly journals Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction

2011 ◽  
Vol 106 (6) ◽  
pp. 763-768 ◽  
Author(s):  
Anahí Souto Vieira ◽  
Grácia Maria Soares Rosinha ◽  
Carina Elisei de Oliveira ◽  
Silvio Arruda Vasconcellos ◽  
Paulo Andre Lima-Borges ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
The State ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Ozotoceros Bezoarticus ◽  
Leptospira Spp ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

scholarly journals Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil

2013 ◽  
Vol 22 (1) ◽  
pp. 136-142 ◽  
Author(s):  
Antonio Thadeu Medeiros Barros ◽  
Teresinha Tizu Sato Schumaker ◽  
Wilson Werner Koller ◽  
Guilherme Marcondes Klafke ◽  
Thais Aguiar de Albuquerque ◽  
...  
Keyword(s):  
Pyrethroid Resistance ◽  
The State ◽  
Kdr Mutation ◽  
Low Frequencies ◽  
Mato Grosso ◽  
Pyrethroid Insecticides ◽  
Resistance Factors ◽  
Horn Fly ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Horn fly resistance to pyrethroid insecticides occurs throughout Brazil, but knowledge about the involved mechanisms is still in an incipient stage. This survey was aimed to identify the mechanisms of horn fly resistance to cypermethrin in Mato Grosso do Sul state, Brazil. Impregnated filter paper bioassays using cypermethrin, synergized or not with piperonyl butoxide (PBO) and triphenyl phosphate (TPP), were conducted from March 2004 to June 2005 in horn fly populations (n = 33) from all over the state. All populations were highly resistant to cypermethrin, with resistance factors (RF) ranging from 89.4 to 1,020.6. Polymerase chain reaction (PCR) assays to detect the knockdown resistance (kdr) mutation also were performed in 16 samples. The kdr mutation was found in 75% of the tested populations, mostly with relatively low frequencies (<20%), and was absent in some highly resistant populations. Addition of TPP did not significantly reduce the LC50 in any population. However, PBO reduced LC50s above 40-fold in all tested populations, resulting in RFs ≤ 10 in most cases. Horn fly resistance to cypermethrin is widespread in the state, being primarily caused by an enhanced activity of P450 mono-oxygenases and secondarily by reduced target site sensitivity.

scholarly journals Epidemics of Soybean Rust (Phakopsora pachyrhizi) in Brazil and Paraguay from 2001 to 2003

Plant Disease ◽  
2005 ◽  
Vol 89 (6) ◽  
pp. 675-677 ◽  
Author(s):  
J. T. Yorinori ◽  
W. M. Paiva ◽  
R. D. Frederick ◽  
L. M. Costamilan ◽  
P. F. Bertagnolli ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sequence Analysis ◽  
Soybean Rust ◽  
Severe Drought ◽  
Phakopsora Pachyrhizi ◽  
Chain Reaction ◽  
Causal Organism ◽  
Mato Grosso ◽  
Polymerase Chain ◽  
The Cost

In 5 March 2001, a severe rust outbreak was recorded at Pitapó, Paraguay, and the causal organism was determined to be Phakopsora pachyrhizi using polymerase chain reaction (PCR) and DNA sequence analysis. In May, rust surveys showed spread throughout most of Paraguay and into western and northern Parana, Brazil. In the 2001-02 season, rust was widespread in Paraguay, but losses were reduced due to severe drought; however, in Brazil it spread to more than 60% of the soybean acreage, causing field losses estimated at 0.1 million metric tons (MMT). In 2003, the disease was observed in more than 90% of the fields in Brazil, and the projected losses in Mato Grosso and Bahia alone are 2.2 MMT (US$487.3 million). Approximately 80% of the soybean acreage in Brazil was sprayed twice with fungicides at the cost of US$544 million. Differences in efficacy have been observed among the commercial strobilurin and triazol fungicides.

scholarly journals Genetic variability of Pantaneiro horse using RAPD-PCR markers

2007 ◽  
Vol 36 (4) ◽  
pp. 799-806 ◽  
Author(s):  
Andréa Alves do Egito ◽  
Beatriz Helena Fuck ◽  
Concepta McManus ◽  
Samuel Rezende Paiva ◽  
Maria do Socorro Maués Albuquerque ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Jaccard Coefficient ◽  
Mato Grosso ◽  
Rapd Pcr ◽  
Close Relationship ◽  
Polymerase Chain ◽  
Genic Diversity ◽  
Mato Grosso Do Sul

Blood samples were collected from Pantaneiro Horses in five regions of Mato Grosso do Sul and Mato Grosso States. Arabian, Mangalarga Marchador and Thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by RAPD-PCR (Random Amplified Polymorphic DNA - Polymerase Chain Reaction) molecular markers. From 146 primers, 13 were chosen for amplification and 44 polymorphic bands were generated. The analysis of molecular variance (AMOVA) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75.47%). Analysis of the genetic variability between pairs of populations presented higher estimates for the five Pantaneiro populations with the Arabian breed, while lowest estimates were presented by pairs formed among the Pantaneiro populations with the Mangalarga Marchador. Highest genic diversity was shown by the Pantaneiro (0.3396), which also showed highest genetic distance with the Arabian and lowest with Mangalarga Marchador breed. UPGMA dendrogram showed distinct differences between naturalized (Pantaneiro and Mangalarga Marchador) and exotic (Arabian and Thoroughbred) breeds. In the dendrogram generated by UPGMA method, the similarity matrix generated by the Jaccard coefficient showed distinction between the naturalised breeds, Pantaneiro and Mangalarga Marchador, and the exotic breeds, Árab and English Thoroughbred. Results suggest that the Pantaneiro presents a higher genetic variability than the other studied breeds and has a close relationship with the Mangalarga Marchador.

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