scholarly journals Difficulties in the diagnosis of HTLV-2 infection in HIV/AIDS patients from Brazil: comparative performances of serologic and molecular assays, and detection of HTLV-2b subtype

2007 ◽  
Vol 49 (4) ◽  
pp. 225-230 ◽  
Author(s):  
Helena Kaminami Morimoto ◽  
Arilson Akira Morimoto ◽  
Edna Maria Vissoci Reiche ◽  
Luiz Toshio Ueda ◽  
Tiemi Matsuo ◽  
...  
Keyword(s):  
Virus Type ◽  
Restriction Analysis ◽  
Serum Samples ◽  
Aids Patients ◽  
Specific Antibodies ◽  
T Lymphotropic Virus ◽  
Molecular Assays ◽  
Pcr Products ◽  
Hiv Aids

The current diagnosis of human T-lymphotropic virus type-2 (HTLV-2) infection is based on the search of specific antibodies; nevertheless, several studies conducted in Brazil pointed deficiencies of the commercially available kits in detecting HTLV-2, mostly in HIV/AIDS patients. This study searched for the presence of HTLV-1 and -2 in 758 HIV/AIDS patients from Londrina, Paraná, Brazil. Serum samples were screened for HTLV-1/2 antibodies using two EIA kits (Vironostika and Murex), and confirmed by WB (HTLV Blot 2.4, Genelabs). The results obtained by EIA disclosed 49 (6.5%) reactive sera: 43 positive by both EIA kits, and six with discordant results. WB confirmed HTLV-1 infection in seven samples (0.9%) and HTLV-2 in 21 sera (2.8%). Negative and indeterminate results were detected in four (0.5%) and 16 (2.1%) sera, respectively. Blood from 47 out of 49 HTLV seroreactive patients were collected and analyzed for the presence of env, LTR and tax genomic segments of HTLVs by PCR. PCR confirmed six cases of HTLV-1 and 37 cases of HTLV-2 infection (14 out of 16 that were found to be WB indeterminate). Restriction analysis of the env PCR products of HTLV-2 disclosed 36 isolates of HTLV-2a/c subtype, and one of HTLV-2b subtype. These results emphasize the need of improving serologic tests for detecting truly HTLV-2 infected patients from Brazil, and confirm the presence of HTLV-2b subtype in the South of this country.

scholarly journals Short Communication: Current Prevalence and Risk Factors Associated with Human T Lymphotropic Virus Type 1 and Human T Lymphotropic Virus Type 2 Infections Among HIV/AIDS Patients in São Paulo, Brazil

2015 ◽  
Vol 31 (5) ◽  
pp. 543-549 ◽  
Author(s):  
Adele Caterino-de-Araujo ◽  
Cláudio Tavares Sacchi ◽  
Maria Gisele Gonçalves ◽  
Karoline Rodrigues Campos ◽  
Mariana Cavalheiro Magri ◽  
...  
Keyword(s):  
Risk Factors ◽  
Virus Type ◽  
Short Communication ◽  
Aids Patients ◽  
T Lymphotropic Virus ◽  
Factors Associated ◽  
Current Prevalence ◽  
Hiv Aids

scholarly journals Comparison of Two Enzyme-Linked Immunosorbent Assays and One Rapid Immunoblot Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Serum

1998 ◽  
Vol 36 (3) ◽  
pp. 845-847 ◽  
Author(s):  
Jan Groen ◽  
Grietje Van Dijk ◽  
Hubert G. M. Niesters ◽  
Willem I. Van Der Meijden ◽  
Albert D. M. E. Osterhaus
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Serum Samples ◽  
Immunoblot Assay ◽  
Specific Antibodies ◽  
Immunosorbent Assays ◽  
Simplex Virus

The sensitivities and specificities of three immunoassays for the detection of herpes simplex virus type 2 (HSV-2)-specific immunoglobulin G antibodies in serum, including the one-strip rapid immunoblot assay (RIBA; Chiron Corporation) and two indirect enzyme immunosorbent assays (EIA; Gull Laboratories and Centocor), were compared by testing a panel of 1,250 serum samples from individuals attending an outpatient clinic for sexually transmitted diseases. A qualitative agreement among the three assays was observed with 1,080 serum samples (86.4%); 291 of the serum samples (23.3%) were positive, 789 samples (63.1%) were negative, and 170 serum samples (13.6%) gave a discordant result. Results were considered conclusive when a concordant result was obtained with two of three assays. The sensitivities and specificities of the RIBA, the Gull EIA, and the Centocor EIA proved to be 99.2, 99.7, and 89.9% and 97.1, 96.7, and 99.3%, respectively. These results indicate that the Chiron RIBA and the Gull EIA are especially useful and reliable for the detection of HSV-2-specific antibodies in serum.

African Origin of Human T-Lymphotropic Virus Type 2 (HTLV-2) Supported by a Potential New HTLV-2d Subtype in Congolese Bambuti Efe Pygmies

1998 ◽  
Vol 72 (5) ◽  
pp. 4327-4340 ◽  
Author(s):  
Anne-Mieke Vandamme ◽  
Marco Salemi ◽  
Marianne Van Brussel ◽  
Hsin-Fu Liu ◽  
Kristel Van Laethem ◽  
...  
Keyword(s):  
T Cell ◽  
Virus Type ◽  
Phylogenetic Analyses ◽  
Enzyme Linked Immunosorbent Assay ◽  
African Origin ◽  
Entire Genome ◽  
T Lymphotropic Virus ◽  
Tax Protein ◽  
Human T Cell

ABSTRACT We identified a potential new subtype within human T-cell lymphotropic virus type 2 (HTLV-2), HTLV-2d, present in members of an isolated Efe Bambuti Pygmy tribe. Two of 23 Efe Pygmies were HTLV-2 seropositive, with HTLV-2 Western blot and enzyme-linked immunosorbent assay reactivities. From one of them the entire genome of the HTLV-2 strain Efe2 could be amplified and sequenced. In all gene regions analyzed, this strain was the most divergent HTLV-2 strain, differing by 2.4% (tax/rex) to 10.7% (long terminal repeat) from both subtypes HTLV-2a and HTLV-2b, yet major functional elements are conserved. The similarity between the HTLV-2 Efe2 Gag and Env proteins and the corresponding HTLV-2a and -2b proteins is consistent with the observed serological reactivity. In the proximal pX region, one of the two alternative splice acceptor sites is abolished in HTLV-2 Efe2. Another interesting feature of this potential new subtype is that it has a Tax protein of 344 amino acids (aa), which is intermediate in length between the HTLV-2a Tax protein (331 aa) and the HTLV-2b and -2c Tax proteins (356 aa) and similar to the simian T-cell lymphotropic virus type 2 (STLV-2) PP1664 Tax protein. Together these two findings suggest a different phenotype for the HTLV-2 Efe2 strain. Phylogenetic analyses confirmed that the Pygmy Efe2 strain potentially belonged to a new and quite divergent subtype, HTLV-2d. When the STLV-2 bonobo viruses PP1664 and PanP were used as an outgroup, it was clear that the Pygmy HTLV-2 Efe2 strain had the longest independent evolution and that HTLV-2 evolution is consistent with an African origin.

scholarly journals Inability to Detect Human T Cell Lymphotropic Virus Type 2-Specific Antibodies in a Patient Coinfected with HIV-1, Human T Cell Lymphotropic Virus Type 1, Human T Cell Lymphotropic Virus Type 2, and Hepatitis C Virus

2014 ◽  
Vol 30 (1) ◽  
pp. 97-101 ◽  
Author(s):  
Adele Caterino-de-Araujo ◽  
Mariana Cavalheiro Magri ◽  
Neuza Satomi Sato ◽  
Helena Kaminami Morimoto ◽  
Luis Fernando de Macedo Brigido ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
T Cell ◽  
Virus Type ◽  
Specific Antibodies ◽  
Human T Cell ◽  
Hiv 1

scholarly journals High Frequencies of Functionally Competent Circulating Tax-Specific CD8+T Cells in Human T Lymphotropic Virus Type 2 Infection

2009 ◽  
Vol 183 (5) ◽  
pp. 2957-2965 ◽  
Author(s):  
André L. A. Oliveira ◽  
Hitoshi Hayakawa ◽  
Doris Schor ◽  
Ana Claudia C. B. Leite ◽  
Otávio M. Espíndola ◽  
...  
Keyword(s):  
T Cells ◽  
Virus Type ◽  
Cd8 T Cells ◽  
High Frequencies ◽  
T Lymphotropic Virus

scholarly journals Antibodies to human T-lymphotropic virus type III (HTLV-III) in saliva of acquired immunodeficiency syndrome (AIDS) patients and in persons at risk for AIDS

Blood ◽  
1986 ◽  
Vol 67 (3) ◽  
pp. 831-834 ◽  
Author(s):  
DW Archibald ◽  
L Zon ◽  
JE Groopman ◽  
MF McLane ◽  
M Essex
Keyword(s):  
At Risk ◽  
Virus Type ◽  
Acquired Immunodeficiency Syndrome ◽  
Serum Antibodies ◽  
Aids Patients ◽  
T Lymphotropic Virus ◽  
Type Iii ◽  
Whole Saliva ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome

Abstract Whole saliva samples collected from available people at risk in Boston for infection with human T-lymphotropic virus type III (HTLV-III/LAV), from late 1984 through early 1985, were analyzed for the presence of antibodies to viral proteins. Fourteen of 20 (70%) acquired immunodeficiency syndrome (AIDS) patients and 14 of 15 (93%) AIDS- related complex (ARC) patients had salivary antibodies that reacted with the virus-encoded glycoproteins gp160 and gp120 of HTLV-III infected cells. All of the AIDS and ARC patients had serum antibodies to the same antigens. Of 20 sex partners of AIDS/ARC patients, nine (45%) showed anti-HTLV-III antibodies, and four of 18 (22%) healthy homosexual males also were positive for such antibodies. Serum and salivary antibody status were the same in these groups. A minority of those patients positive for salivary antibodies to env gene-encoded gp160 and gp120 also had salivary antibodies to gag gene-encoded proteins of 55,000, 24,000, and/or 17,000 daltons. Immunoglobulin A (IgA) class antibodies comprised the majority of the salivary antibody response. The spectrum of HTLV-III proteins detected by the salivary and serum antibodies was similar. The possibility that secretory IgA from the gut-associated lymphoid system may play a role to restrict salivary transmission of HTLV-III should be considered.

scholarly journals ASUHAN KEPERAWATAN PADA Nn. G.B DAN Tn.M.B.A YANG MENGALAMI HIV/AIDS DENGAN MASALAH PERUBAHAN MEMBRAN MUKOSA ORAL DI RUANG MELATI DAN FLAMBOYAN RSUD Mgr. GABRIEL MANEK, SVD ATAMBUA

2020 ◽  
Vol 2 (02) ◽  
pp. 18-32
Author(s):  
Arny Munika Manafe ◽  
Maria Fatimah W. A Fouk ◽  
Melkianus Ratu
Keyword(s):  
Human Immunodeficiency Virus ◽  
Virus Type ◽  
Acquired Immunodeficiency Syndrome ◽  
T Lymphotropic Virus ◽  
Type Iii ◽  
Immunodeficiency Virus ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome ◽  
Hiv Aids

Latar belakang : Virus yang di kenal sebagai Human T Lymphotropic Virus Type III ( HTLV III ) atau sekarang yang lasim di sebut Human Immunodeficiency Virus ( HIV ). HIV dapat berkembang lebih lanjut menjadi Acquired Immunodeficiency Syndrome (AIDS). Acquire Immunodefiency Sydrom (AIDS  adalah kumpulan gejala penyakit akibat menurunnya sistem kekebalan tubuh oleh virus. Tujuan : Mahasiswa mampu memberikan tindakan bagaiamana perawatan pada pasien HIV/AIDS dengan Perubahan Membran Mukosa Oral yang meliputi pengkajian, diagnosa  keperawatan, perencanaan, pelaksanan dan evaluasi. Metode penelitian: Studi kasus yaitu studi yang mengekspolarasikan suatu masalah asuhan keperawatan pada Nn.G.B dan Tn. M.B.A yang mengalami HIV/AIDS dengan perubahan membran mukosa oral. Hasil: Setelah diberikan Asuhan keperawatan pada Nn. G.B dan Tn. M.B.A dengan perubahan membran mukosa oralyang sudah teratasi. Kesimpulan : Berdasarkan pembahasan pada bab IV  terdahulu maka penulis mengambil kesimpulan dan saran pada Nn.G.B dan Tn.M.B.A dengan proses keperawatan yang terdiri dari Pengkajian, Diagnosa keperawatan, Perencanaan, Pelaksanaan dan Evaluasi masalah teratasi.

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