scholarly journals Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil

2008 ◽  
Vol 50 (3) ◽  
pp. 139-143 ◽  
Author(s):  
Ana Julia Urias dos Santos Araújo ◽  
Herminia Yohko Kanamura ◽  
Marcos Eduardo de Almeida ◽  
Aparecida Helena de Souza Gomes ◽  
Thais Helena Lemos Pinto ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Genetic Characterization ◽  
Cryptosporidium Oocyst ◽  
Sao Paulo ◽  
São Paulo ◽  
Oocyst Wall ◽  
Cryptosporidium Hominis ◽  
Stool Samples ◽  
Dna Fragment ◽  
Genotypic Identification

Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the Sate of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP), for genetic characterization. The analysis was based on digestion with RsaI restriction enzyme of a DNA fragment amplified from the Cryptosporidium oocyst wall protein (COWP) gene. Based on this analysis, four samples were identified as Cryptosporidium parvum, eight as Cryptosporidium hominis and two presented a profile that correspondedto Cryptosporidium meleagridis when compared to the standards used in the analysis. The use of molecular methods can be helpful to identify source of infections and risk factors related to Cryptosporidium infection in our communities.

scholarly journals Genomic Variation in IbA10G2 and Other Patient-Derived Cryptosporidium hominis Subtypes

2016 ◽  
Vol 55 (3) ◽  
pp. 844-858 ◽  
Author(s):  
Per Sikora ◽  
Sofia Andersson ◽  
Jadwiga Winiecka-Krusnell ◽  
Björn Hallström ◽  
Cecilia Alsmark ◽  
...  
Keyword(s):  
Parasite Burden ◽  
Genomic Variation ◽  
Loss Of Function ◽  
Oocyst Wall ◽  
Gene Encoding ◽  
Cryptosporidium Hominis ◽  
Content Type ◽  
Successful Isolation ◽  
Target Dna ◽  
Stool Samples

ABSTRACTIn order to improve genotyping and epidemiological analysis ofCryptosporidiumspp., genomic data need to be generated directly from a broad range of clinical specimens. Utilizing a robust method that we developed for the purification and generation of amplified target DNA, we present its application for the successful isolation and whole-genome sequencing of 14 differentCryptosporidium hominispatient specimens. Six isolates of subtype IbA10G2 were analyzed together with a single representative each of 8 other subtypes: IaA20R3, IaA23R3, IbA9G3, IbA13G3, IdA14, IeA11G3T3, IfA12G1, and IkA18G1. Parasite burden was measured over a range of more than 2 orders of magnitude for all samples, while the genomes were sequenced to mean depths of between 17× and 490× coverage. Sequence homology-based functional annotation identified several genes of interest, including the gene encodingCryptosporidiumoocyst wall protein 9 (COWP9), which presented a predicted loss-of-function mutation in all the sequence subtypes, except for that seen with IbA10G2, which has a sequence identical to theCryptosporidium parvumreference Iowa II sequence. Furthermore, phylogenetic analysis showed that all the IbA10G2 genomes form a monophyletic clade in theC. hoministree as expected and yet display some heterogeneity within the IbA10G2 subtype. The current report validates the aforementioned method for isolating and sequencingCryptosporidiumdirectly from clinical stool samples. In addition, the analysis demonstrates the potential in mining data generated from sequencing multiple whole genomes ofCryptosporidiumfrom human fecal samples, while alluding to the potential for a higher degree of genotyping withinCryptosporidiumepidemiology.

scholarly journals Enteroparasitic occurrence in fecal samples analyzed at the University of Western São Paulo-UNOESTE clinical laboratory, Presidente Prudente, São Paulo State, Brazil

2004 ◽  
Vol 46 (5) ◽  
pp. 243-248 ◽  
Author(s):  
Nair Toshiko Tashima ◽  
Maria Jacira Silva Simões
Keyword(s):  
Giardia Lamblia ◽  
Clinical Laboratory ◽  
Clinical Signs ◽  
Data Bank ◽  
Sao Paulo ◽  
São Paulo ◽  
Bank Structure ◽  
Stool Samples ◽  
Medical Attendance ◽  
The University

This study aims to analyze the enteroparasitic occurrence in children from 0 to 12 years old consulted at the University of western São Paulo Clinical Laboratory, Presidente Prudente, SP, Brazil, in relation to the socioeconomic profile of the attended children. Stool samples were examined and a questionnaire was applied with the objective of knowing the patient's age, sex, medical attendance, characteristic of the habitation, provisioning of water, dejection and domestic waste fates, use of footwear and clinical signs. The software EPI INFO 6 (Version 6.04b) was used for the elaboration of the data bank structure and analysis after previous data codification. Among 1,000 children analyzed, as many as 21.3% presented some kind of parasite. The most frequent protozoan was Giardia lamblia (7.3%) followed by Entamoeba coli (3.9%). The most frequent helminth was Enterobius vermicularis (1.9%) followed by Hymenolepis nana (0.5%). The most frequent protozoan association was Giardia lamblia / Entamoeba coli (0.9%).

scholarly journals Meningococcal Disease Caused by Neisseria meningitidis Serogroup B Serotype 4 in São Paulo, Brazil, 1990 to 1996

1998 ◽  
Vol 40 (2) ◽  
pp. 65-70 ◽  
Author(s):  
Claudio Tavares Sacchi ◽  
Ana Paula Silva de Lemos ◽  
Maria Claudia C. Camargo ◽  
Anne M. Whitney ◽  
Carmo Elias A. Melles ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Meningococcal Disease ◽  
Fragment Length Polymorphism ◽  
Systemic Disease ◽  
Sao Paulo ◽  
Frequent Pattern ◽  
Rrna Genes ◽  
São Paulo ◽  
Vaccination Trial ◽  
Prevalent Strain

A large epidemic of serogroup B meningococcal disease (MD), has been occurring in greater São Paulo, Brazil, since 1988.21 A Cuban-produced vaccine, based on outer-membrane-protein (OMP) from serogroup B: serotype 4: serosubtype P1.15 (B:4:P1.15) Neisseria meningitidis, was given to about 2.4 million children aged from 3 months to 6 years during 1989 and 1990. The administration of vaccine had little or no measurable effects on this outbreak. In order to detect clonal changes that could explain the continued increase in the incidence of disease after the vaccination, we serotyped isolates recovered between 1990 and 1996 from 834 patients with systemic disease. Strains B:4:P1.15, which was detected in the area as early as 1977, has been the most prevalent phenotype since 1988. These strains are still prevalent in the area and were responsible for about 68% of 834 serogroup B cases in the last 7 years. We analyzed 438 (52%) of these strains by restriction fragment length polymorphism (RFLPs) of rRNA genes (ribotyping). The most frequent pattern obtained was referred to as Rb1 (68%). We concluded that the same clone of B:4:P1.15-Rb1 strains was the most prevalent strain and responsible for the continued increase of incidence of serogroup B MD cases in greater São Paulo during the last 7 years in spite of the vaccination trial.

Genetic characterization of 32 X-InDels in a population sample from São Paulo State (Brazil)

2019 ◽  
Vol 133 (5) ◽  
pp. 1385-1388 ◽  
Author(s):  
Juliana Martinez ◽  
Fernanda Silva Polverari ◽  
Flávia Alves de Jesus Silva ◽  
Danilo Faustino Braganholi ◽  
Joyce Aparecida Martins Lopes Ferraz ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Population Sample ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State

Genetic characterization of Rabies virus isolated from cattle between 1997 and 2002 in an epizootic area in the state of São Paulo, Brazil

2009 ◽  
Vol 144 (1-2) ◽  
pp. 215-224 ◽  
Author(s):  
Pedro Carnieli ◽  
Juliana Galera Castilho ◽  
Willian de Oliveira Fahl ◽  
Nazle Mendonça Collaço Véras ◽  
Maria do Carmo Sampaio Tavares Timenetsky
Keyword(s):  
Rabies Virus ◽  
Genetic Characterization ◽  
Sao Paulo ◽  
The State ◽  
São Paulo

scholarly journals Genotypic Characterization of Cryptosporidium hominis from Water Samples in São Paulo, Brazil

2011 ◽  
Vol 85 (5) ◽  
pp. 834-838 ◽  
Author(s):  
Ronalda S. Araújo ◽  
Rodrigo M. Soares ◽  
Maria Helena Matté ◽  
Maria Inês Z. Sato ◽  
Glavur R. Matté ◽  
...  
Keyword(s):  
Water Samples ◽  
Sao Paulo ◽  
São Paulo ◽  
Cryptosporidium Hominis ◽  
Genotypic Characterization

scholarly journals Caracterização molecular através da técnica fAFLP de isolados de Diaporthe citri

2006 ◽  
Vol 32 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Márcia de Holanda Nozaki ◽  
Margarete Camargo ◽  
Eliana Gertrudes Macedo Lemos ◽  
Ana Paula de Andrade Aukar ◽  
Modesto Barreto
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Sao Paulo ◽  
São Paulo

A citricultura é um mercado em expansão, principalmente no Estado de São Paulo, cuja importância na balança comercial já é reconhecida. Como em qualquer espécie cultivada, o crescimento das áreas de cultivo favorecem também o crescimento de problemas fitossanitários. Desta forma, as espécies de citros são afetadas por diversas doenças destacando-se entre elas a melanose, causada por Diaporthe citri (Wolf.), à qual a grande maioria das variedades comerciais são suscetíveis. O conhecimento da diversidade intra-específica é de grande importância, já que esta poderá auxiliar na seleção de variedades com resistência. O objetivo deste trabalho foi avaliar a variabilidade genética em isolados de Diaporthe citri, originários de diferentes locais, variedades e partes da planta, utilizando marcadores moleculares. Marcadores do tipo AFLP (Amplified Fragment Length Polymorphism) foram utilizados para caracterização de dez isolados do patógeno. Os DNAs genômicos extraídos da massa micelial foram utilizados nas reações de amplificação. A técnica fluorescent AFLP permitiu a distinção dos isolados estudados, tendo sido classificados em quatro grupos distintos. Contudo, estes grupos não foram formados em razão da região geográfica, parte da planta ou variedade.

scholarly journals Genetic characterization of rabies virus isolated from bovines and equines between 2007 and 2008, in the States of São Paulo and Minas Gerais

2010 ◽  
Vol 43 (2) ◽  
pp. 116-120 ◽  
Author(s):  
Carla Isabel Macedo ◽  
Pedro Carnieli Junior ◽  
Willian de Oliveira Fahl ◽  
Jonas Yoshitaka de Oliveira Lima ◽  
Rafael de Novaes Oliveira ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Genetic Characterization ◽  
Active Regions ◽  
Minas Gerais ◽  
Sao Paulo ◽  
Amino Acid Sequences ◽  
Biologically Active ◽  
Economic Losses ◽  
São Paulo

INTRODUCTION: Rabies is an acute disease of the central nervous system and is responsible for the deaths of thousands of humans, wild animals and livestock, particularly cattle, as well as causing major economic losses. This study describes the genetic characterization of rabies virus variants that circulate in Desmodus rotundus populations and are transmitted to herbivores. METHODS: Fifty rabies virus isolates from bovines and equines in the States of São Paulo and Minas Gerais, Brazil, were genetically characterized and compared with sequences retrieved from GenBank. RESULTS: Two clusters (I and II) with mean nucleotide identities of 99.1 and 97.6% were found. The first of these contained nearly all the samples analyzed. Lineages from other Brazilian states grouped in cluster II. CONCLUSIONS: Analysis of the amino acid sequences of the N proteins revealed the existence of genetic markers that may indicate possible variations between geographic regions, although the biologically active regions are conserved within the species over space and time.

scholarly journals Genetic characterization of St. Louis encephalitis virus isolated from human in São Paulo, Brazil

2006 ◽  
Vol 101 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Cecília Luiza Simões dos Santos ◽  
Maria Anice Mureb Sallum ◽  
Heitor Moreira Franco ◽  
Fabíola Maiumi Oshiro ◽  
Iray Maria Rocco
Keyword(s):  
Genetic Characterization ◽  
Sao Paulo ◽  
Encephalitis Virus ◽  
São Paulo

scholarly journals Expression and antigenic analysis of the recombinant TRP36 protein from Ehrlichia canis São Paulo strain for serologic tests

2020 ◽  
Vol 29 (3) ◽  
Author(s):  
Miguel Ângelo da Silva Medeiros ◽  
Maria Helena da Silva ◽  
Maria Adelaide do Valle Matta ◽  
Eliane de Oliveira Ferreira ◽  
Sérgio Lisboa Machado ◽  
...  
Keyword(s):  
Serological Test ◽  
Sao Paulo ◽  
Ehrlichia Canis ◽  
São Paulo ◽  
Serum Samples ◽  
Antigenic Analysis ◽  
Bacterial Dna ◽  
Canine Monocytic Ehrlichiosis ◽  
Dna Fragment ◽  
Topo Vector

Abstract Ehrlichia canis is the main etiological agent of canine monocytic ehrlichiosis (CME), a globally canine infectious disease. In Brazil, CME is considered to be endemic, and its prevalence can reach 65% in some states. The diagnosis of ehrlichiosis is important for treatment and epidemiological purposes. The E. canis TRP36 (Tandem Repeat Protein) protein elicits the earliest acute-phase antibody response observed during the course of the disease. This study aimed to generate the recombinant TRP36 protein from E. canis São Paulo strain and to evaluate its potential as a tool for the serologic diagnosis of CME. The E. canis São Paulo isolate was cultivated in DH82 lineage cells, and its genomic DNA was obtained. The bacterial DNA fragment encoding the entire ORF of TRP36 was cloned into the pBAD/Thio-TOPO vector and transformed into Escherichia coli DH10B competent cells with the trp36-bearing plasmid for protein expression. To evaluate the protein antigenicity, 16 canine serum samples were previously tested (by PCR and the commercial SNAP®4Dx® serological test). The results were in accordance with the SNAP®4Dx® test. Experiments using this recombinant protein as an antigen, targeting the development of a serologic test based on ELISA methodology, are the next step to produce a reliable, affordable and useful diagnostic tool for CME in Brazil.

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