scholarly journals Expression and antigenic analysis of the recombinant TRP36 protein from Ehrlichia canis São Paulo strain for serologic tests

2020 ◽  
Vol 29 (3) ◽  
Author(s):  
Miguel Ângelo da Silva Medeiros ◽  
Maria Helena da Silva ◽  
Maria Adelaide do Valle Matta ◽  
Eliane de Oliveira Ferreira ◽  
Sérgio Lisboa Machado ◽  
...  
Keyword(s):  
Serological Test ◽  
Sao Paulo ◽  
Ehrlichia Canis ◽  
São Paulo ◽  
Serum Samples ◽  
Antigenic Analysis ◽  
Bacterial Dna ◽  
Canine Monocytic Ehrlichiosis ◽  
Dna Fragment ◽  
Topo Vector

Abstract Ehrlichia canis is the main etiological agent of canine monocytic ehrlichiosis (CME), a globally canine infectious disease. In Brazil, CME is considered to be endemic, and its prevalence can reach 65% in some states. The diagnosis of ehrlichiosis is important for treatment and epidemiological purposes. The E. canis TRP36 (Tandem Repeat Protein) protein elicits the earliest acute-phase antibody response observed during the course of the disease. This study aimed to generate the recombinant TRP36 protein from E. canis São Paulo strain and to evaluate its potential as a tool for the serologic diagnosis of CME. The E. canis São Paulo isolate was cultivated in DH82 lineage cells, and its genomic DNA was obtained. The bacterial DNA fragment encoding the entire ORF of TRP36 was cloned into the pBAD/Thio-TOPO vector and transformed into Escherichia coli DH10B competent cells with the trp36-bearing plasmid for protein expression. To evaluate the protein antigenicity, 16 canine serum samples were previously tested (by PCR and the commercial SNAP®4Dx® serological test). The results were in accordance with the SNAP®4Dx® test. Experiments using this recombinant protein as an antigen, targeting the development of a serologic test based on ELISA methodology, are the next step to produce a reliable, affordable and useful diagnostic tool for CME in Brazil.

scholarly journals An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses

2004 ◽  
Vol 34 (5) ◽  
pp. 1525-1529 ◽  
Author(s):  
Cristiane Divan Baldani ◽  
Rosangela Zacarias Machado ◽  
Paulo de Tarso Landgraf Botteon ◽  
Felipe Santoro Takakura ◽  
Carlos Luiz Massard
Keyword(s):  
Immunosorbent Assay ◽  
Serological Test ◽  
Epidemiological Studies ◽  
Sao Paulo ◽  
Enzyme Linked Immunosorbent Assay ◽  
São Paulo ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Babesia Equi ◽  
Specificity And Sensitivity

A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in the field and that equine piroplasmosis is a cause for serious concern in the State of São Paulo, Brazil.

scholarly journals Line Immunoassay for Confirmation and Discrimination of Human T-Cell Lymphotropic Virus Infections in Inconclusive Western Blot Serum Samples from Brazil

2019 ◽  
Vol 58 (1) ◽  
Author(s):  
Karoline R. Campos ◽  
Fred L. N. Santos ◽  
Vanessa da Silva Brito ◽  
Noilson L. S. Gonçalves ◽  
Thessika H. A. Araujo ◽  
...  
Keyword(s):  
T Cell ◽  
Western Blot ◽  
Serological Test ◽  
Sao Paulo ◽  
São Paulo ◽  
Serum Samples ◽  
Aids Patients ◽  
Human T Cell ◽  
Specificity And Sensitivity ◽  
Hiv Aids

ABSTRACT Difficulties in confirming and discriminating human T-cell lymphotropic virus type 1 (HTLV-1) and HTLV-2 infections by serological Western blot (WB) assays (HTLV Blot 2.4; MP Biomedicals) have been reported in Brazil, mainly in HIV/AIDS patients, with a large number of WB-indeterminate and WB-positive but HTLV-untypeable results. Nonetheless, a line immunoassay (LIA) (INNO-LIA HTLV-I/II; Fujirebio) provided enhanced specificity and sensitivity for confirming HTLV-1/2 infections. To add information concerning the improved ability of the LIA in relation to WB when applied to samples of individuals from different risk groups from Brazil, we performed the present study. Three groups were analyzed: group 1 (G1), with 62 samples from HIV/AIDS patients from São Paulo, SP (48 WB indeterminate and 14 HTLV untypeable); group 2 (G2), with 24 samples from patients with hepatitis B or hepatitis C from São Paulo (21 WB indeterminate and 3 HTLV untypeable; 17 HIV seropositive); and group 3 (G3), with 25 samples from an HTLV outpatient clinic in Salvador, Bahia (16 WB indeterminate and 9 HTLV untypeable; all HIV seronegative). Overall, the LIA confirmed HTLV-1/2 infection (HTLV-1, HTLV-2, or HTLV) in 66.1% (G1), 83.3% (G2), and 76.0% (G3) of samples. Interestingly, the majority of WB-indeterminate results were confirmed by the LIA as being HTLV-2 positive in G1 and G2 but not in G3, in which the samples were defined as being HTLV-1 or HTLV positive. These results agree with the virus types that circulate in such patients of different regions in Brazil and emphasize that the LIA is the best serological test for confirming HTLV-1 and HTLV-2 infections, independently of being applied in HTLV-monoinfected or HTLV-coinfected individuals.

scholarly journals Epidemiological aspects of the Brazilian spotted fever: serological survey of dogs and horses in an endemic area in the State of São Paulo, Brazil

1996 ◽  
Vol 38 (6) ◽  
pp. 427-430 ◽  
Author(s):  
Elba R.S. de Lemos ◽  
Raimundo D. Machado ◽  
José R. Coura ◽  
Maria A.A.M. Guimarães ◽  
Nelson Chagas
Keyword(s):  
Endemic Area ◽  
Spotted Fever ◽  
Domestic Animals ◽  
Sao Paulo ◽  
Spotted Fever Group ◽  
São Paulo ◽  
Serological Survey ◽  
Serum Samples ◽  
Blood Samples ◽  
Brazilian Spotted Fever

In order to obtain information on Brazilian spotted fever, a study in domestic animals was performed in the County of Pedreira, State of São Paulo, Brazil, where 17 human cases had been notified. Serum samples obtained from animals were tested by indirect immunofluorescence for detectable antibodies to spotted fever-group rickettsiae. Seropositivity was revealed in 12 (36.4%) of 33 dogs and seven (77.8%) of nine horses from the endemic area. For comparison, blood samples from dogs and horses from non endemic area were tested and four (12.9%) of 31 dogs and three (27.3%) of 11 horses were positive. The highest titers of antibodies by IFA (IgG > 1:1024) were found only in three dogs and six horses from endemic area. The results suggest that dogs as horses may serve as environmental sentinels for estabilishing the prevalence of foci of spotted fever in Brazil.

scholarly journals Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil

2008 ◽  
Vol 50 (3) ◽  
pp. 139-143 ◽  
Author(s):  
Ana Julia Urias dos Santos Araújo ◽  
Herminia Yohko Kanamura ◽  
Marcos Eduardo de Almeida ◽  
Aparecida Helena de Souza Gomes ◽  
Thais Helena Lemos Pinto ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Genetic Characterization ◽  
Cryptosporidium Oocyst ◽  
Sao Paulo ◽  
São Paulo ◽  
Oocyst Wall ◽  
Cryptosporidium Hominis ◽  
Stool Samples ◽  
Dna Fragment ◽  
Genotypic Identification

Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the Sate of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP), for genetic characterization. The analysis was based on digestion with RsaI restriction enzyme of a DNA fragment amplified from the Cryptosporidium oocyst wall protein (COWP) gene. Based on this analysis, four samples were identified as Cryptosporidium parvum, eight as Cryptosporidium hominis and two presented a profile that correspondedto Cryptosporidium meleagridis when compared to the standards used in the analysis. The use of molecular methods can be helpful to identify source of infections and risk factors related to Cryptosporidium infection in our communities.

scholarly journals Toxoplasma gondii antibodies and related risk factors in mammals at Sorocaba zoo, São Paulo, BrazilToxoplasma gondii antibodies and related risk factors in mammals at Sorocaba zoo, São Paulo, Brazil

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2845
Author(s):  
Renata Bezerra Marujo ◽  
Helio Langoni ◽  
Leila Sabrina Ullmann ◽  
Maysa Pellizzaro ◽  
Ramiro Das Neves Dias Neto ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Feeding Habits ◽  
Univariate Analysis ◽  
Multivariate Logistic Regression Analysis ◽  
Sao Paulo ◽  
São Paulo ◽  
Intermediate Hosts ◽  
Serum Samples ◽  
Related Risk

Toxoplasmosis is a parasitic zoonosis with worldwide distribution and different wild species either are involved in maintenance of the pathogen in the environment as definitive or intermediate hosts serving as font of feeding or prey to definitive hosts. The present study aimed to investigate Toxoplasma gondii antibodies and the risk factors relating to infection in mammals at Sorocaba Zoo, state of São Paulo, Brazil. Serum samples collected from 153 animals were analyzed using the modified agglutination test (MAT; cutoff ? 25). Seropositivity was found in 62 animals (40.5%; 95% CI: 33.1-48.5%), with different titers. Significant differences were observed in relation to the ages of the animals, origin, presence of free-ranging animals in the enclosure and feeding habits, through univariate analysis (p ? 0.05). Multivariate logistic regression analysis showed that only age (p = 0.03) had significance for the study. Adult animals were 3.5 more likely to become infected than were young ones. In relation to feeding habits, herbivores (80%) and carnivores (46.5%) were the animals most affected. These results highlight the presence of T. gondii in animals at Brazilian zoos, and suggest that continuous transmission is occurring at zoos.

scholarly journals The measurement by serological means of the impact of the Hong Kong/68 influenza virus on a population

1972 ◽  
Vol 6 (1) ◽  
pp. 85-88 ◽  
Author(s):  
José Alberto N. Candeias ◽  
Marguerite S. Pereira
Keyword(s):  
Influenza Virus ◽  
Hong Kong ◽  
Adult Population ◽  
Haemagglutination Inhibition ◽  
Sharp Decrease ◽  
Sao Paulo ◽  
First Year ◽  
São Paulo ◽  
Serum Samples ◽  
The Impact

In order to obtain evidence on the size of the impact of the Hong Kong/68 variant of influenza A2 virus on the population of São Paulo, Brazil, serum samples taken in 1967 before this variant appeared and during successive years after it appeared were examined for their antibody content. Haemagglutination-inhibition tests performed on a total of 2726 serum samples from adults showed a sharp decrease in 1969 of the proportion of sera without antibody to the Hong Kong/68 variant and a corresponding mercase in the proportion with high titres. It was concluded that about three-quarters of the adult population became infected at some time after the variant appeared, the majority in the first year of prevalence.

scholarly journals Toxoplasma gondii antibodies and related risk factors in mammals at Sorocaba zoo, São Paulo, BrazilToxoplasma gondii antibodies and related risk factors in mammals at Sorocaba zoo, São Paulo, Brazil

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2845 ◽  
Author(s):  
Renata Bezerra Marujo ◽  
Helio Langoni ◽  
Leila Sabrina Ullmann ◽  
Maysa Pellizzaro ◽  
Ramiro Das Neves Dias Neto ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Feeding Habits ◽  
Univariate Analysis ◽  
Multivariate Logistic Regression Analysis ◽  
Sao Paulo ◽  
São Paulo ◽  
Intermediate Hosts ◽  
Serum Samples ◽  
Related Risk

Toxoplasmosis is a parasitic zoonosis with worldwide distribution and different wild species either are involved in maintenance of the pathogen in the environment as definitive or intermediate hosts serving as font of feeding or prey to definitive hosts. The present study aimed to investigate Toxoplasma gondii antibodies and the risk factors relating to infection in mammals at Sorocaba Zoo, state of São Paulo, Brazil. Serum samples collected from 153 animals were analyzed using the modified agglutination test (MAT; cutoff ? 25). Seropositivity was found in 62 animals (40.5%; 95% CI: 33.1-48.5%), with different titers. Significant differences were observed in relation to the ages of the animals, origin, presence of free-ranging animals in the enclosure and feeding habits, through univariate analysis (p ? 0.05). Multivariate logistic regression analysis showed that only age (p = 0.03) had significance for the study. Adult animals were 3.5 more likely to become infected than were young ones. In relation to feeding habits, herbivores (80%) and carnivores (46.5%) were the animals most affected. These results highlight the presence of T. gondii in animals at Brazilian zoos, and suggest that continuous transmission is occurring at zoos.

scholarly journals Prevalence of rubella antibodies in a non-immunized urban population, São Paulo, Brazil

1994 ◽  
Vol 36 (4) ◽  
pp. 373-376 ◽  
Author(s):  
Vanda A.U.F. Souza ◽  
José Cassio Moraes ◽  
Laura M Sumita ◽  
Maria Claudia C. Camargo ◽  
Maria Cristina D.S. Fink ◽  
...  
Keyword(s):  
Vaccination Campaign ◽  
Public Health Problem ◽  
Sao Paulo ◽  
Mass Vaccination ◽  
São Paulo ◽  
Serum Samples ◽  
Important Public Health Problem ◽  
Rubella Infection ◽  
Cord Serum ◽  
Mass Vaccination Campaign

The prevalence of rubella antibodies was evaluated through a ramdom Seroepidemiological survey in 1400 blood samples of 2-14 year old children and in 329 samples of umbilical cord serum. Rubella IgG antibodies were detected by ELISA, and the sera were collected in 1987, five years before the mass vaccination campaign with measles-mumps-rubella vaccine carried out in the city of São Paulo in 1992. A significant increase in prevalence of rubella infection was observed after 6 years of age, and 77% of the individuals aged from 15 to 19 years had detectable rubella antibodies. However, the seroprevalence rose to 90.5% (171/189) in cord serum samples from children whose mothers were 20 to 29 years old, and reached 95.6% in newborns of mothers who were 30 to 34 years old, indicating that a large number of women are infected during childbearing years. This study confirms that rubella infection represents an important Public Health problem in São Paulo city. The data on the seroprevalence of rubella antibodies before the mass vaccination campaign reflects the baseline immunological status of this population before any intervention and should be used to design an adequate vaccination strategy and to assess the Seroepidemiological impact of this intervention.

scholarly journals Neospora caninum: soroepidemiologia de vacas e cães de propriedades leiteiras de Cunha, São Paulo, Brasil

2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 3123
Author(s):  
Sintia Barboza Bastos ◽  
Thais Agostinho Martins ◽  
Ana Sue Sammi ◽  
Felippe Danyel Cardoso Martins ◽  
Ivo Alexandre Leme da Cunha ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Neospora Caninum ◽  
Horizontal Transmission ◽  
Sao Paulo ◽  
Economic Losses ◽  
Primary Study ◽  
Second Phase ◽  
São Paulo ◽  
Serum Samples ◽  
Blood Samples

Neosporosis, a parasitic disease prevalent worldwide, is caused by the protozoan Neospora caninum, an obligatory intracellular parasite that can cause abortions and infertility in females, resulting in high economic losses. The aim of this study was to determine the seroprevalence of antibodies against N. caninum in dairy cattle, and the occurrence of antibodies in dogs from farms in the municipality of Cunha, São Paulo, Brazil. Additionally, eighteen months after the primary study, an analysis to determine the incidence, seroconversion, and occurrence of anti-N. caninum antibodies was conducted in cows. Sampling was carried out in two phases: during the first one, 400 dairy cattle and 50 dog blood samples from 40 farms were collected. During the second phase, blood samples were collected from 119 cows belonging to 16 farms visited 18 months before. Serum samples were analyzed by indirect immunofluorescence (IFI) with titers ? 100 for cattle and ? 50 for dogs considered as positives. The occurrence of antibodies against N. caninum was 15.0% (60/400) and 14.0% (7/50) for cattle and dogs, respectively. After 18 months, the observed incidence of antibodies was 7.4% (4/54), the conversion detected was 71.4% (seropositive to seronegative) and 7.4% (seronegative to seropositive animals), and the observed occurrence was 7.56% (9/119). The calculated odds ratio (OR) was significant (p < 0.05) for abortions, presence of dogs, and parturition stalls. However, protection factors were identified in 2- to 4-year-old dairy cattle and in the number of lactating cows ? 10 animals. Thus, the presence of antibodies against N. caninum in the studied suggested both vertical and horizontal transmission are occurring in cattle.

Sign in / Sign up

Export Citation Format

Share Document