scholarly journals Serosurvey of hantavirus infection in humans in the border region between Brazil and Argentina

2011 ◽  
Vol 44 (2) ◽  
pp. 131-135 ◽  
Author(s):  
William Marciel de Souza ◽  
Alex Martins Machado ◽  
Luiz Tadeu Moraes Figueiredo ◽  
Everton Boff
Keyword(s):  
Immunofluorescence Assay ◽  
Farm Workers ◽  
Border Region ◽  
Hantavirus Pulmonary Syndrome ◽  
Hantavirus Infection ◽  
Western Region ◽  
Igg Antibodies ◽  
Farm Worker ◽  
Indirect Contact ◽  
N Protein

INTRODUCTION: According to reports by the Ministry of Health, in the far western region of the State of Santa Catarina, there have been no reports of hantavirus pulmonary syndrome, a zoonotic disease transmitted by feces of infected rodents. A seroepidemiological study of residents of this region, was conducted, with the aim of determining the presence of hantavirus infections. A total of 340 volunteers of both genus, from the towns of Belmonte and Paraíso, were studied. METHODS: The serum of these patients was collected and used to detect IgG antibodies against recombinant N protein of Araraquara hantavirus, by ELISA assay. The positive samples were then titrated and confirmed by immunofluorescence assay. RESULTS: This study demonstrated the presence of IgG antibodies against hantavirus N protein in 3.5% of the population. The most frequent occupation was farm worker, 81% had direct and indirect contact with rodents, 91.7% of positive cases were farm workers, indicating that the probable cause of infection occurred during barn cleaning. These antibodies are noteworthy, given that the levels of antibodies were verified in individuals whose contact with hantavirus may have occurred many years ago. CONCLUSIONS: This study shows the circulation of hantavirus in the region, a fact that until now, had not reported. All the serum reagents had contact with the pathogen, but did not develop pulmonary and cardiovascular syndrome. It is important to remain alert, because hantavirus is a serious and emerging disease of some relevance.

scholarly journals Longitudinal analysis of virus load, serum antibody levels and virus neutralizing activity in vitro in cases with less severe COVID-19

2020 ◽  
Author(s):  
Bertram Flehmig ◽  
Michael Schindler ◽  
Natalia Ruetalo ◽  
Ramona Businger ◽  
Manfred Bayer ◽  
...  
Keyword(s):  
Serum Antibody ◽  
Disease Onset ◽  
Igg Antibodies ◽  
Virus Load ◽  
Neutralizing Activity ◽  
N Protein ◽  
Life Threatening ◽  
Antibody Levels ◽  
The Relationship

Background: Patients infected with SARS-CoV-2 exhibit a highly variable clinical course, varying from barely discernible signs of disease, to moderate flu-like symptoms and, occasionally, with life-threatening pneumonia and/or cytokine storm. The relationship between the nasopharyngeal virus load, IgA and IgG antibodies to both the S1-RBD-protein and the N-protein as well the neutralizing activity (NAbs) against SARS-CoV-2 in the blood of moderately afflicted COVID-19 patients has not been investigated longitudinally so far. Methods: Several new serological methods to examine these parameters were developed and validated for the longitudinal investigation in three patients of a family which underwent a mild course of COVID-19. Findings: We observed that the virus load had almost completely disappeared after about four weeks, whereas serum antibodies showed a contrasting course. IgA levels to S1-RBD-protein and, to a lesser extent, to the N-protein, peaked about three weeks after clinical disease onset but declined soon thereafter. IgG levels rose continuously, reaching a plateau approximately six weeks after disease onset. NAbs in serum reached a peak about four weeks after disease onset but dropped to a lower level about six weeks later. Interpretation: Our data establishes associations of virus neutralization and a serological immune response foremost against Sars-CoV-2 S1-RDB-protein in a longitudinal manner.

scholarly journals Notes from the Field: Hantavirus Pulmonary Syndrome in a Migrant Farm Worker — Colorado, 2016

2017 ◽  
Vol 66 (02) ◽  
pp. 62-63 ◽  
Author(s):  
Grace Marx ◽  
Kaylan Stinson ◽  
Monte Deatrich ◽  
Bernadette Albanese
Keyword(s):  
Hantavirus Pulmonary Syndrome ◽  
Farm Worker ◽  
Migrant Farm

scholarly journals Serum-Positive and -Negative AQP4 Antibody NMO in Chinese Patients

2012 ◽  
Vol 39 (2) ◽  
pp. 232-235 ◽  
Author(s):  
Youming Long ◽  
Zhengqi Lu ◽  
Xueqiang Hu
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Assay ◽  
Chinese Patients ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Positive Antibody ◽  
A Cell ◽  
The Central Nervous System ◽  
Aqp4 Antibody ◽  
Virchow Robin Space

Objective:To compare the clinical features of our sero-negative and sero-positive neuromyelitis optica (NMO) patients.Methods:Thirty-nine patients with NMO were recruited and analyzed retrospectively. Serum aquaporin 4 (AQP4) antibody status was determined by a cell-based assay. For the sero-negative patients, cerebrospinal fluid (CSF) and serum samples were re-tested using the cell-based assay and an indirect immunofluorescence assay.Results:By the cell-based assay, 30 patients (76.92%, 30/39), were positive for AQP4 antibodies in serum and 37 patients (94.9%, 37/39), had a CSF-positive antibody status. Seven NMO patients (17.9%, 7/39) were sero-negative by the cell-based assay but demonstrated positive CSF results. By indirect immunofluorescence, the remaining two patients, who had no AQP4 antibodies in serum or CSF by the cell-based assay, were positive for IgG antibodies in serum, which selectively targeted the central nervous system microvessels, pia, subpia, Virchow-Robin space, kidney, and stomach. There were no significant differences between the sero-positive and sero-negative NMO groups among their demographic and clinical data.Conclusions:Repeating the test using a different assay or CSF is helpful to clarify whether sero-negative NMO patients do in fact carry AQP4 antibodies.

scholarly journals Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

2015 ◽  
Vol 96 (7) ◽  
pp. 1664-1675 ◽  
Author(s):  
Jiaxin Ling ◽  
Anne Jääskeläinen ◽  
Satu Hepojoki ◽  
Jussi Hepojoki ◽  
Heikki Henttonen ◽  
...  
Keyword(s):  
Cross Reaction ◽  
Hantavirus Infection ◽  
Puumala Virus ◽  
Serological Survey ◽  
N Protein

Hantavirus Infection Induces a Typical Myocarditis That May Be Responsible for Myocardial Depression and Shock in Hantavirus Pulmonary Syndrome

2007 ◽  
Vol 195 (10) ◽  
pp. 1541-1549 ◽  
Author(s):  
Fabiano P. Saggioro ◽  
Marcos A. Rossi ◽  
Maria Irma S. Duarte ◽  
Carmen Cinira S. Martin ◽  
Venâncio A. F. Alves ◽  
...  
Keyword(s):  
Hantavirus Pulmonary Syndrome ◽  
Hantavirus Infection ◽  
Myocardial Depression

scholarly journals Comparative analysis of neutrophil gelatinase-associated lipocalin and other laboratory markers for lupus nephritis: a cross-sectional investigation

2016 ◽  
Vol 54 (2) ◽  
pp. 240-245 ◽  
Author(s):  
Sonia L La’ulu ◽  
Brenda B Suh-Lailam ◽  
K Wayne Davis ◽  
Joely A Straseski ◽  
Anne E Tebo
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Renal Involvement ◽  
Immunofluorescence Assay ◽  
Igg Antibodies ◽  
Systemic Lupus ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Double Stranded Dna ◽  
Neutrophil Gelatinase

Background Lupus nephritis is one of the most serious complications of systemic lupus erythematosus. This study evaluates the prevalence and correlation between neutrophil gelatinase-associated lipocalin and other biomarkers associated with renal involvement in systemic lupus erythematosus. Methods Paired serum and urine specimens from 50 suspected systemic lupus erythematosus patients, characterized by antinuclear antibodies detected by indirect immunofluorescence assay and varying positive concentrations of anti-double stranded DNA antibodies by Crithidia luciliae immunofluorescence assay, were investigated. Of these 50 patients, 18 were identified with renal involvement based upon laboratory serology. Patients and healthy control serum samples ( n = 50) were also evaluated for high avidity double stranded DNA IgG antibodies, anti-C1q IgG antibodies, and serum creatinine. The prevalence and relationship between biomarkers were evaluated using statistical methods. Results Serum and urine neutrophil gelatinase-associated lipocalin concentrations were significantly elevated in patients compared to controls, with a prevalence of 24% and 36%, respectively. These concentrations were also more markedly increased in systemic lupus erythematosus patients with renal involvement than those without. Spearman’s correlations between neutrophil gelatinase-associated lipocalin and other biomarkers tested ranged from 0.06 to 0.66 in all patients. Combined concordance as determined by Cronbach alpha coefficient between biomarkers was reduced from 0.71 to 0.58 (serum) and 0.62 (urine) when neutrophil gelatinase-associated lipocalin was removed. Conclusions Neutrophil gelatinase-associated lipocalin concentrations are elevated and demonstrate variable associated with other laboratory markers for renal involvement in systemic lupus erythematosus. Prospective longitudinal studies are needed to determine the optimal biomarker combinations for use in routine management of systemic lupus erythematosus patients at-risk for lupus nephritis.

scholarly journals Cytomegalovirus infection in children with Down syndrome in a day-care center in Brazil

2000 ◽  
Vol 42 (4) ◽  
pp. 179-184 ◽  
Author(s):  
Cynthia L. Motta do CANTO ◽  
Celso F. H. GRANATO ◽  
Elisa GARCEZ ◽  
Lucy S. VILLAS BOAS ◽  
M. Cristina D. S. FINK ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Day Care ◽  
Care Center ◽  
Immunofluorescence Assay ◽  
Igg Antibodies ◽  
Cmv Infection ◽  
Day Care Center ◽  
Children With Down Syndrome ◽  
Human Foreskin Fibroblasts ◽  
Virus Culture

This study evaluates the transmission of CMV infection in 120 children aged 1 to 15 years with Down syndrome who attended a day-care center for handicapped children in São Paulo, Brazil. A blood sample was obtained from each children at the beginning of the study for detection of IgG and IgM cytomegalovirus (CMV) antibodies by an immunofluorescence assay. Samples of saliva and urine were obtained every 3 months from the children with CMV antibodies to detect shedding of the virus by culture in human foreskin fibroblasts, by detection of pp65 CMV-antigen and by a nested PCR assay. The prevalence of anti CMV-IgG antibodies was 76.6% (92/120), and IgM anti-CMV antibodies were detected in 13% (12/92) of the seropositive children. During the first viral evaluation, CMV was detected in the urine and/or saliva in 39/90 (43.3%) of the seropositive children. In the second and third evaluations, CMV was detected in 41/89 (46%) and in 35/89 (39.3%) children, respectively. Detection of CMV was shown both in urine and saliva in 28/39 (71.8%), 19/41(46.3%) and 20/35 (57.1%) of the children excreting the virus, respectively. Additionally, in 33/49 (67.4%) of the excreters CMV could be demonstrated in urine or saliva in at least two out of the three virological evaluations carried out sequentially in a six month period. Of the 28 initially seronegative children, 26 were re-examined for anti-CMV IgG antibodies about 18 months after the negative sample; seroconversion was found in 10/26 (38.5%). Taking all 536 samples of urine or saliva examined by virus culture and pp65 antigen detection during the study into account, 159 (29.6%) were positive by virus culture and 59 (11%) gave a positive result with the pp65 assay. These data demonstrate the high prevalence of CMV shedding and the high risk of CMV infection in children with Down syndrome attending a day-care center for mentally handicapped patients. The virus culture was more sensitive than the pp65 CMV antigen assay for CMV detection in both urine and saliva samples.

scholarly journals In-House Immunofluorescence Assay for Detection of SARS-CoV-2 Antigens in Cells from Nasopharyngeal Swabs as a Diagnostic Method for COVID-19

Diagnostics ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2346
Author(s):  
Athene Hoi-Ying Lam ◽  
Jian-Piao Cai ◽  
Ka-Yi Leung ◽  
Ricky-Ruiqi Zhang ◽  
Danlei Liu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Nucleocapsid Protein ◽  
Diagnostic Method ◽  
Polyclonal Antibodies ◽  
Respiratory Viruses ◽  
Cross Reactivity ◽  
Immunofluorescence Assay ◽  
Respiratory Epithelial Cells ◽  
N Protein ◽  
Respiratory Epithelial

Immunofluorescence is a traditional diagnostic method for respiratory viruses, allowing rapid, simple and accurate diagnosis, with specific benefits of direct visualization of antigens-of-interest and quality assessment. This study aims to evaluate the potential of indirect immunofluorescence as an in-house diagnostic method for SARS-CoV-2 antigens from nasopharyngeal swabs (NPS). Three primary antibodies raised from mice were used for immunofluorescence staining, including monoclonal antibody against SARS-CoV nucleocapsid protein, and polyclonal antibodies against SARS-CoV-2 nucleocapsid protein and receptor-binding domain of SARS-CoV-2 spike protein. Smears of cells from NPS of 29 COVID-19 patients and 20 non-infected individuals, and cells from viral culture were stained by the three antibodies. Immunofluorescence microscopy was used to identify respiratory epithelial cells with positive signals. Polyclonal antibody against SARS-CoV-2 N protein had the highest sensitivity and specificity among the three antibodies tested, detecting 17 out of 29 RT-PCR-confirmed COVID-19 cases and demonstrating no cross-reactivity with other tested viruses except SARS-CoV. Detection of virus-infected cells targeting SARS-CoV-2 N protein allow identification of infected individuals, although accuracy is limited by sample quality and number of respiratory epithelial cells. The potential of immunofluorescence as a simple diagnostic method was demonstrated, which could be applied by incorporating antibodies targeting SARS-CoV-2 into multiplex immunofluorescence panels used clinically, such as for respiratory viruses, thus allowing additional routine testing for diagnosis and surveillance of SARS-CoV-2 even after the epidemic has ended with low prevalence of COVID-19.

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