Experimental infection of Lutzomyia longipalpis fed on a patient with cutaneous leishmaniasis due to Leishmania mexicana amazonensis

The authors were able to infect phlebotomine sandflies on a human case of American Cutaneous Leishmaniasis by feeding females of Lutzomyia longipalpis on a patient with a lesion due to Leishmania mexicana amazonensis.

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Sand fly captures with Disney traps in area of occurrence of Leishmania (Leishmania) amazonensis in the state of Mato Grosso do Sul, mid-western Brazil

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822010000500003 ◽

2010 ◽

Vol 43 (5) ◽

pp. 491-495 ◽

Cited By ~ 15

Author(s):

Maria Elizabeth Cavalheiros Dorval ◽

Tulia Peixoto Alves ◽

Geucira Cristaldo ◽

Hilda Carlos da Rocha ◽

Murilo Andrade Alves ◽

...

Keyword(s):

Natural Infection ◽

Leishmania Species ◽

Leishmania Amazonensis ◽

Sand Fly ◽

Lutzomyia Longipalpis ◽

Mato Grosso ◽

Predominant Species ◽

American Cutaneous Leishmaniasis ◽

Phlebotomine Sandflies ◽

Mato Grosso Do Sul

INTRODUCTION: The work was conducted to study phlebotomine fauna (Diptera: Psychodidae) and aspects of American cutaneous leishmaniasis transmission in a forested area where Leishmania (Leishmania) amazonensis occurs, situated in the municipality of Bela Vista, State of Mato Grosso do Sul, Brazil. METHODS: The captures were conducted with modified Disney traps, using hamster (Mesocricetus auratus) as bait, from May 2004 to January 2006. RESULTS: Ten species of phlebotomine sandflies were captured: Brumptomyia avellari, Brumptomyia brumpti, Bichromomyia flaviscutellata, Evandromyia bourrouli, Evandromyia lenti, Lutzomyia longipalpis, Psathyromyia campograndensis, Psathyromyia punctigeniculata, Psathyromyia shannoni and Sciopemyia sordellii. The two predominant species were Ev bourrouli (57.3%) and Bi flaviscutellata (41.4%), present at all sampling sites. Two of the 36 hamsters used as bait presented natural infection with Leishmania. The parasite was identified as Leishmania (Leishmania) amazonensis. CONCLUSIONS: Analysis of the results revealed the efficiency of Disney traps for capturing Bichromomyia flaviscutellata and the simultaneous presence of both vector and the Leishmania species transmitted by the same can be considered a predictive factor of the occurrence of leishmaniasis outbreaks for the human population that occupies the location.

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Leishmania mexicana in Proechimys iheringi denigratus Moojen (Rodentia, Echimyidae) in a region endemic for American cutaneous leishmaniasis

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86821985000400006 ◽

1985 ◽

Vol 18 (4) ◽

pp. 243-246 ◽

Cited By ~ 5

Author(s):

Air C. Barretto ◽

Norman E. Peterson ◽

Ednaldo Lago ◽

Ana C. Rosa ◽

Rosely S.M. Braga ◽

...

Keyword(s):

Monoclonal Antibodies ◽

First Record ◽

Skin Tissue ◽

Lutzomyia Longipalpis ◽

Leishmania Mexicana ◽

Isoenzyme Analysis ◽

The Third ◽

American Cutaneous Leishmaniasis ◽

Forest Region ◽

Apparently Healthy

Three isolates of Leishmania were recovered from five of 27 specimens of the rodent Proechimys iheringi denigratus Moojen captured near Três Braços in the Atlantic Forest region of Bahia, Brazil. Two of these isolates were recovered from hamsters inoculated with a pooled triturate of liver, spleen and skin tissue from apparently healthy P. i. denigratus. The third isolate was recovered from a triturate of only skin tissue from another. Metastasis was observed in the inoculated hamsters, the parasites grew abundantly in artificial media and a typical suprapylarial pattern of infection in Lutzomyia longipalpis was produced indicating that the parasites belong to the Leishmania mexicana complex. All isolates reacted with Leishmania mexicana mexicana and Leishmania mexicana amazonensis monoclonal antibodies. The isoenzyme analysis differentiated these isolates from standard isolates of L. m. mexicana, L. m. amazonensis, L. m. aristedesi, L. m. pifanoi, L. m. garnhami and L. m. ssp.(Goiás-W. Barbosa). These isolates seem to be a subspecies of L. mexicana very closely related to L. m. amazonensis from which they differ by decreased electrophoretic mobility of GPI, PEP and ALAT. This is the first record of the isolation of a parasite of thegenus Leishmania in a rodent captured in the State of Bahia.

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Preliminary study towards a novel experimental model to study localized cutaneous leishmaniasis caused bY Leishmania (Leishmania) mexicana

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652012000300009 ◽

2012 ◽

Vol 54 (3) ◽

pp. 165-170 ◽

Cited By ~ 7

Author(s):

Erika Ivett Sosa-Bibiano ◽

Nicole Raymonde Van Wynsberghe ◽

Silvia Beatriz Canto-Lara ◽

Fernando Jose Andrade-Narvaez

Keyword(s):

Cutaneous Leishmaniasis ◽

Experimental Model ◽

Clinical Sign ◽

Experimental Infection ◽

Granulomatous Inflammation ◽

Control Group ◽

Leishmania Mexicana ◽

Histological Features ◽

Histological Pattern ◽

Preliminary Study

There is not an experimental model of localized cutaneous leishmaniasis (LCL) caused by Leishmania (Leishmania) mexicana. The aim of the present study was to characterize the clinical and histological features of Peromyscus yucatanicus experimentally infected with L. (L.) mexicana. A total of 54 P. yucatanicus (groups of 18) were inoculated with 1x10(6) promastigotes of L. (L.) mexicana in the base of the tail. They were euthanized at three and six months post experimental infection. The control group was inoculated with RPMI-1640. The predominant clinical sign observed was a single ulcerated lesion in 27.77% (5/18) and in 11.11% (2/18) P. yucatanicus at three and six months respectively. The histological pattern described as chronic granulomatous inflammation with or without necrosis was found in 7/7 (100%) biopsies of euthanized P. yucatanicus at three (n = 5) and six (n = 2) months, respectively. These results resembled clinical and histological features caused by L. (L.) mexicana in humans, and support the possibility to employ P. yucatanicus as a novel experimental model to study LCL caused by this parasite.

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American cutaneous leishmaniasis: presentation and problems of patient management

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86821988000400002 ◽

1988 ◽

Vol 21 (4) ◽

pp. 165-172 ◽

Cited By ~ 5

Author(s):

Jeffrey D. Chulay ◽

Charles N. Oster ◽

Patrick B. McGreevy ◽

Larry D. Hendricks ◽

Richard D. Kreutzer

Keyword(s):

Cutaneous Leishmaniasis ◽

Incubation Period ◽

Patient Management ◽

Leishmania Donovani ◽

Skin Lesions ◽

Medical Attention ◽

Leishmania Braziliensis ◽

Leishmania Mexicana ◽

American Cutaneous Leishmaniasis ◽

The Mean

We report our experience with the diagnosis and treatment of 60 patients with American cutaneous leishmaniasis. They were infected in Panama (55), Brazil (4) or Colombia (I). Among 35 patients with a 3 week exposure in Panama, the mean maximum incubation period was 33 days (range 4-81 days). Diagnosis was delayed an average of 93 days after onset of skin lesions, due to the patient's delay in seeking medical attention (31 days), medical personnel's delay in considering the diagnosis (45 days), and the laboratory's delay in confirming the diagnosis (17 days). Forty-four patients (73%) developed ulcers typical of cutaneous leishmaniasis. Sixteen additional patients (27%) had atypical macular, papular, squamous, verrucous or acneiform skin lesions that were diagnosed only because leishmanial cultures were obtained. Of the 59 patients treated with pentavalent antimonial drugs, only 34 (58%) were cured after the first course of treatment. Lesions which were at least 2 cm in diameter, ulcerated, or caused by Leishmania braziliensis were less likely to be cured after a single course of treatment than were lesions smaller than 2 cm, nonulcerated or caused by Leishmania mexicana or Leishmania donovani.

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Attractiveness of black and white modified Shannon traps to phlebotomine sandflies (Diptera, Psychodidae) in the Brazilian Amazon Basin, an area of intense transmission of American cutaneous leishmaniasis

Parasite ◽

10.1051/parasite/2017021 ◽

2017 ◽

Vol 24 ◽

pp. 20 ◽

Cited By ~ 6

Author(s):

Andreia Fernandes Brilhante ◽

Márcia Moreira de Ávila ◽

Jailson Ferreira de Souza ◽

Antônio Ralph Medeiros-Sousa ◽

Priscila Bassan Sábio ◽

...

Keyword(s):

Cutaneous Leishmaniasis ◽

Amazon Basin ◽

Brazilian Amazon ◽

Black And White ◽

American Cutaneous Leishmaniasis ◽

Phlebotomine Sandflies

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Cutaneous factors in susceptibility to American cutaneous leishmaniasis

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1016/0035-9203(87)90312-9 ◽

1987 ◽

Vol 81 (1) ◽

pp. 160 ◽

Cited By ~ 1

Author(s):

P. Esterre ◽

P.R. Ridel ◽

P. Jamet ◽

J.P. Dedet

Keyword(s):

Cutaneous Leishmaniasis ◽

American Cutaneous Leishmaniasis

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On the vertical distribution of Phlebotomine sandflies (Dipt., Psychodidae) in British Honduras (Belize)

Bulletin of Entomological Research ◽

10.1017/s000748530000362x ◽

1970 ◽

Vol 59 (4) ◽

pp. 637-646 ◽

Cited By ~ 17

Author(s):

Paul Williams

Keyword(s):

Ground Level ◽

Insect Host ◽

Leishmania Mexicana ◽

Light Traps ◽

Principal Vector ◽

New Information ◽

Phlebotomine Sandflies ◽

British Honduras ◽

The Vertical Distribution ◽

Blood Meals

Phlebotomine sandflies were collected simultaneously at ground level, 25 ft and 40 ft in an area of medium bush in British Honduras (Belize).Flies were captured on human bait, with rat-baited oil-traps and with miniature light-traps, and some resting individuals were collected at each level. One species of Brumptomyia França and 18 of Lutzomyia França & Parrot were obtained. Brief notes on some of these species are given.Miniature light-traps provided the widest range of species, were the most satisfactory means for collecting Brumptomyia, L. steatopyga (Fairchild & Hertig) and L. carpenteri (Fairchild & Hertig), and yielded new information on the flight activities of L. deleoni (Fairchild & Hertig) and L. panamensis (Shannon).Most species collected were found to be predominantly arboreal in habit, L. permira (Fairchild & Hertig) and Lutzomyia sp. P being almost exclusively so. Some species (L. panamensis, possibly L. geniculata (Mangabeira) and L. bispinosa (Fairchild & Hertig)) live amongst the foliage but descend to the forest floor to seek blood-meals. A few species (L. steatopyga, L. deleoni and L. olmeca (Vargas & Díaz Nájera)) are active mainly near the ground, though the occasional specimen may be carried much higher.L. olmeca is the principal vector of rodent leishmaniasis in British Honduras. In the present study, no evidence was obtained to incriminate any other species of Phlebotomine as an insect host for Leishmania mexicana, and the mode of transmission of the parasite to man remains obscure.

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