Humoral Immune Response Kinetics in Philander opossum and Didelphis marsupialis Infected and Immunized by Trypanosoma cruzi Employing an Immunofluorescence Antibody Test

Based on 92537 blood samples received from 7365 patients with a request for a meningococcal antibody test (MAT) during a 10-year period (1986–1995), the usefulness of the test in the diagnosis of meningococcal disease was assessed. Of 635 patients with culture-confirmed meningococcal disease, 88% were seronegative on admittance to hospital and 90% were seropositive 10–15 days after onset of disease. The humoral immune response in children <2 years of age was weaker than in older children and adults. Among 893 MAT-positive patients without culture-confirmed meningococcal disease, 261 (29%) had been notified as cases of meningococcal disease. Among 228 patients notified as serologically confirmed the MAT results were consistent with the clinical diagnosis in 86%. MAT is a reliable tool for establishing a diagnosis in patients with suspected meningococcal disease. Key factors facilitating appropriate interpretation of negative as well as positive test results were: time(s) of sampling(s) after onset of disease, age of the patient and clinical features.

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Pregnancy and humoral immune response in mice chronically infected by Trypanosoma cruzi.

Infection and Immunity ◽

10.1128/iai.55.10.2496-2501.1987 ◽

1987 ◽

Vol 55 (10) ◽

pp. 2496-2501 ◽

Cited By ~ 20

Author(s):

Y Carlier ◽

M T Rivera ◽

C Truyens ◽

M Goldman ◽

P Lambert ◽

...

Keyword(s):

Immune Response ◽

Trypanosoma Cruzi ◽

Humoral Immune Response ◽

Humoral Immune

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DNA Immunization with Trypanosoma cruzi HSP70 Fused to the KMP11 Protein Elicits a Cytotoxic and Humoral Immune Response against the Antigen and Leads to Protection

Infection and Immunity ◽

10.1128/iai.69.10.6558-6563.2001 ◽

2001 ◽

Vol 69 (10) ◽

pp. 6558-6563 ◽

Cited By ~ 69

Author(s):

Lourdes Planelles ◽

M. Carmen Thomas ◽

Carlos Alonso ◽

Manuel C. López

Keyword(s):

Immune Response ◽

T Lymphocytes ◽

Trypanosoma Cruzi ◽

Cytotoxic T Lymphocytes ◽

Humoral Immune Response ◽

Chimeric Gene ◽

Dna Immunization ◽

Humoral Immune ◽

Parasite Challenge

ABSTRACT Murine immunization with Trypanosoma cruzi KMP11-HSP70fused genes but not the KMP11 gene alone elicited both an immunoglobulin G2a long-lasting humoral immune response against KMP11 protein and activation of CD8+ cytotoxic T lymphocytes specific for two KMP11 peptides containing A2 motifs. Moreover, protection against the parasite challenge was observed after immunization with the chimeric gene.

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Specific cellular and humoral immune response in Balbc mice immunised with an expression genomic library of Trypanosoma cruzi

Vaccine ◽

10.1016/s0264-410x(97)00228-4 ◽

1998 ◽

Vol 16 (6) ◽

pp. 608-612 ◽

Cited By ~ 23

Author(s):

Esteban Alberti ◽

Armando Acosta ◽

Maria E. Sarmiento ◽

Carlos Hidalgo ◽

Teresita Vidal ◽

...

Keyword(s):

Immune Response ◽

Trypanosoma Cruzi ◽

Humoral Immune Response ◽

Genomic Library ◽

Humoral Immune

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Kinetics of the Humoral Immune Response in Mice Experimentally Infected with Trypanosoma Cruzi

Clinical Science ◽

10.1042/cs066076p_pt2 ◽

1984 ◽

Vol 66 (2) ◽

pp. 76P-76P

Author(s):

P. Hindmarsh ◽

L. Hudson ◽

R.C.K. Wong

Keyword(s):

Immune Response ◽

Trypanosoma Cruzi ◽

Humoral Immune Response ◽

Humoral Immune ◽

Kinetics Of

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Trypanosoma cruzi in marsupial didelphids (Philander frenata and Didelhis marsupialis): differences in the humoral immune response in natural and experimental infections

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822003000200008 ◽

2003 ◽

Vol 36 (2) ◽

pp. 241-248 ◽

Cited By ~ 9

Author(s):

Ana Paula Legey ◽

Ana Paula Pinho ◽

Samanta C.C. Xavier ◽

Renato Marchevsky ◽

João Carlos Carreira ◽

...

Keyword(s):

Immune Response ◽

Trypanosoma Cruzi ◽

Western Blot ◽

Humoral Immune Response ◽

Immunofluorescence Assay ◽

Evolutionary Divergence ◽

Humoral Immune ◽

Recognition Pattern ◽

Sds Page

Philander frenata and Didelphis marsupialis harbor parasitism by Trypanosoma cruzi without developing any apparent disease and on the contrary to D. marsupialis, P. frenata maintains parasitism by T. cruzi II subpopulations. Here we compared the humoral immune response of the two didelphids naturally and experimentally infected with T. cruzi II group, employing SDS-PAGE/Western blot techniques and by an Indirect immunofluorescence assay. We also studied the histopathological pattern of naturally and experimentally infected P. frenata with T. cruzi. P. frenata sera recognized more antigens than D. marsupialis, and the recognition pattern did not show any change over the course of the follow up of both didelphid species. Polypeptides of 66 and 90kDa were the most prominent antigens recognized by both species in the soluble and enriched membrane fractions. P. frenata recognized intensely also a 45kDa antigen. Our findings indicate that: 1) there were no quantitative or qualitative differences in the patent or subpatent phases in the recognition pattern of P. frenata; 2) the significant differences in the recognition pattern of parasitic antigens by P. frenata and D. marsupialis sera suggest that they probably "learned" to live in harmony with T. cruzi by different strategies; 3) although P. frenata do not display apparent disease, tissular lesions tended to be more severe than has been described in D. marsupialis; and 4) Both didelphids probably acquired infection by T. cruzi after their evolutionary divergence.

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Longitudinal evaluation of humoral immune response and merozoite surface antigen diversity in calves naturally infected with Babesia bovis, in São Paulo, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612017069 ◽

2017 ◽

Vol 26 (4) ◽

pp. 479-490 ◽

Cited By ~ 3

Author(s):

Carlos António Matos ◽

Luiz Ricardo Gonçalves ◽

Dasiel Obregón Alvarez ◽

Carla Roberta Freschi ◽

Jenevaldo Barbosa da Silva ◽

...

Keyword(s):

Genetic Diversity ◽

Immune Response ◽

Surface Antigen ◽

Humoral Immune Response ◽

Fluorescent Antibody ◽

Antibody Test ◽

Babesia Bovis ◽

Enzyme Linked Immunosorbent Assay ◽

Humoral Immune ◽

Merozoite Surface Antigen

Abstract Babesiosis is an economically important infectious disease affecting cattle worldwide. In order to longitudinally evaluate the humoral immune response against Babesia bovis and the merozoite surface antigen diversity of B. bovis among naturally infected calves in Taiaçu, Brazil, serum and DNA samples from 15 calves were obtained quarterly, from their birth to 12 months of age. Anti-B. bovis IgG antibodies were detected by means of the indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). The polymerase chain reaction (PCR) was used to investigate the genetic diversity of B. bovis, based on the genes that encode merozoite surface antigens (MSA-1, MSA-2b and MSA-2c). The serological results demonstrated that up to six months of age, all the calves developed active immunity against B. bovis. Among the 75 DNA samples evaluated, 2, 4 and 5 sequences of the genes msa-1, msa-2b and msa-2c were obtained. The present study demonstrated that the msa-1 and msa-2b genes sequences amplified from blood DNA of calves positive to B. bovis from Taiaçu were genetically distinct, and that msa-2c was conserved. All animals were serologically positive to ELISA and IFAT, which used full repertoire of parasite antigens in despite of the genetic diversity of MSAs.

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