scholarly journals The meningococcal antibody test: how useful in the diagnosis of meningococcal disease?

2004 ◽  
Vol 133 (2) ◽  
pp. 217-227 ◽  
Author(s):  
N. WEIS ◽  
L. BERTHELSEN ◽  
H. WACHMANN ◽  
I. LIND
Keyword(s):  
Immune Response ◽  
Clinical Features ◽  
Meningococcal Disease ◽  
Humoral Immune Response ◽  
Antibody Test ◽  
Test Results ◽  
Older Children ◽  
Key Factors ◽  
Blood Samples ◽  
Humoral Immune

Based on 92537 blood samples received from 7365 patients with a request for a meningococcal antibody test (MAT) during a 10-year period (1986–1995), the usefulness of the test in the diagnosis of meningococcal disease was assessed. Of 635 patients with culture-confirmed meningococcal disease, 88% were seronegative on admittance to hospital and 90% were seropositive 10–15 days after onset of disease. The humoral immune response in children <2 years of age was weaker than in older children and adults. Among 893 MAT-positive patients without culture-confirmed meningococcal disease, 261 (29%) had been notified as cases of meningococcal disease. Among 228 patients notified as serologically confirmed the MAT results were consistent with the clinical diagnosis in 86%. MAT is a reliable tool for establishing a diagnosis in patients with suspected meningococcal disease. Key factors facilitating appropriate interpretation of negative as well as positive test results were: time(s) of sampling(s) after onset of disease, age of the patient and clinical features.

scholarly journals Humoral immune response in beef heifers supplemented with mineral salt with or without the addition of rumen-protected methionine

2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 3057
Author(s):  
Matheus Gomes Lopes ◽  
José Henrique Echenique Dominguez ◽  
Cristina Mendes Peter ◽  
Ederson Santos ◽  
Paula Almeida Rodrigues ◽  
...  
Keyword(s):  
Immune Response ◽  
Treatment Group ◽  
Humoral Immune Response ◽  
Vaccine Dose ◽  
Daily Weight Gain ◽  
Control Group ◽  
Beef Heifers ◽  
Blood Samples ◽  
Humoral Immune ◽  
Mineral Supplementation

The aim of this study was to evaluate the humoral immune response in beef heifers supplemented with mineral supplementation with or without the addition of rumen-protected methionine. Forty-eight Brangus nulliparous heifers were distributed into four experimental groups with three replications each: control group without supplementation and without vaccination (CG01), control group without supplementation and with vaccination (CG02), treatment group with mineral supplementation and vaccination (TG01), and treatment group with mineral supplementation added with protected methionine and vaccination (TG02). The animals were maintained under native pasture with access to water ad libitum and the supplementation was available in high-consumption covered troughs. A supplementation period of 60 days prior to vaccinations was adopted until the first dose of a monovalent experimental vaccine inactivated for BoHV-5 was applied as a method of stimulating the immune response to evaluate the supplementation effects. After a 21-day interval, blood samples were collected to evaluate the humoral response and the second vaccine booster dose was applied following the 21-day interval for new blood samples in order to evaluate the immune response against the two-vaccination protocol. From the beginning of the experiment, the animals were weighed on the days ?60, ?10, 0, 21, and 42 in relation to the vaccine protocol. The experimental groups did not differ for body weight, mean daily weight gain, and body condition score after 102 days of supplementation regardless of the treatment. No animals belonging to CG01 seroconverted throughout the experiment, proving that there was no introduction of the agent (BoHV) in the studied area. When vaccinated animals were compared to the CG01 control group, statistically higher levels of neutralizing antibodies (P ? 0.0001) and IgG (P ? 0.0001) were verified 21 days after the second vaccine dose. Among the animals of the three vaccinated groups, there was no difference in seroconversion and IgG production. Therefore, no benefits of mineral supplementation or enriched with protected methionine were observed for the humoral immune response of the studied animals.

scholarly journals Longitudinal evaluation of humoral immune response and merozoite surface antigen diversity in calves naturally infected with Babesia bovis, in São Paulo, Brazil

2017 ◽  
Vol 26 (4) ◽  
pp. 479-490 ◽  
Author(s):  
Carlos António Matos ◽  
Luiz Ricardo Gonçalves ◽  
Dasiel Obregón Alvarez ◽  
Carla Roberta Freschi ◽  
Jenevaldo Barbosa da Silva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Immune Response ◽  
Surface Antigen ◽  
Humoral Immune Response ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Babesia Bovis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Humoral Immune ◽  
Merozoite Surface Antigen

Abstract Babesiosis is an economically important infectious disease affecting cattle worldwide. In order to longitudinally evaluate the humoral immune response against Babesia bovis and the merozoite surface antigen diversity of B. bovis among naturally infected calves in Taiaçu, Brazil, serum and DNA samples from 15 calves were obtained quarterly, from their birth to 12 months of age. Anti-B. bovis IgG antibodies were detected by means of the indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). The polymerase chain reaction (PCR) was used to investigate the genetic diversity of B. bovis, based on the genes that encode merozoite surface antigens (MSA-1, MSA-2b and MSA-2c). The serological results demonstrated that up to six months of age, all the calves developed active immunity against B. bovis. Among the 75 DNA samples evaluated, 2, 4 and 5 sequences of the genes msa-1, msa-2b and msa-2c were obtained. The present study demonstrated that the msa-1 and msa-2b genes sequences amplified from blood DNA of calves positive to B. bovis from Taiaçu were genetically distinct, and that msa-2c was conserved. All animals were serologically positive to ELISA and IFAT, which used full repertoire of parasite antigens in despite of the genetic diversity of MSAs.

Role of Yersinia pseudotuberculosis outer proteins (Yops) in murine humoral immune response

2009 ◽  
Vol 54 (3) ◽  
pp. 239-245 ◽  
Author(s):  
J. M. L. Maia ◽  
L. G. S. Monnazzi ◽  
B. M. M. Medeiros
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Yersinia Pseudotuberculosis ◽  
Humoral Immune

scholarly journals Diagnostic and analytical performance evaluation of ten commercial assays for detecting SARS-CoV-2 humoral immune response

2021 ◽  
pp. 113043
Author(s):  
Marnix Mylemans ◽  
Eveline Van Honacker ◽  
Louis Nevejan ◽  
Stefanie van den Bremt ◽  
Laura Hofman ◽  
...  
Keyword(s):  
Immune Response ◽  
Performance Evaluation ◽  
Humoral Immune Response ◽  
Analytical Performance ◽  
Humoral Immune
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