scholarly journals Sugarcane yellow leaf virus infection leads to alterations in photosynthetic efficiency and carbohydrate accumulation in sugarcane leaves

2005 ◽  
Vol 30 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Marcos C. Gonçalves ◽  
Jorge Vega ◽  
Jurandi G. Oliveira ◽  
Mara M. A. Gomes
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Apparatus ◽  
Defense Responses ◽  
Physiological Status ◽  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Leaf Virus ◽  
Leaf Symptoms ◽  
Severe Leaf

Infection by Sugarcane yellow leaf virus (ScYLV) causes severe leaf symptoms in sugarcane (Saccharum spp.) hybrids, which indicate alterations in its photosynthetic apparatus. To gain an overview of the physiological status of infected plants, we evaluated chlorophyll a fluorescence and gas exchange assays, correlating the results with leaf metabolic surveys, i.e., photosynthetic pigments and carbohydrate contents. When compared to healthy plants, infected plants showed a reduction in potential quantum efficiency for photochemistry of photosystem (PSII) and alterations in the filling up of the plastoquinone (PQ) pool. They also showed reduction in the CO2 net exchange rates, probably as a consequence of impaired quantum yield. In addition, reductions were found in the contents of photosynthetic leaf pigments and in the ratio chlorophyll a/chlorophyll b (chla/chlb). Carbohydrate content in the leaves was increased as a secondary effect of the ScYLV infection. This article discusses the relation of virus replication and host defense responses with general alterations in the photosynthetic apparatus and in the metabolism of infected plants.

scholarly journals First Report of Sugarcane Yellow Leaf Virus in the French West Indies

Plant Disease ◽  
1999 ◽  
Vol 83 (6) ◽  
pp. 588-588 ◽  
Author(s):  
J. H. Daugrois ◽  
I. Jean-Baptiste ◽  
B. E. L. Lockhart ◽  
M. S. Irey ◽  
M. Chatenet ◽  
...  
Keyword(s):  
West Indies ◽  
Causal Agent ◽  
Spherical Particles ◽  
Yellow Leaf ◽  
French West Indies ◽  
Sugarcane Yellow Leaf Virus ◽  
Two Samples ◽  
Leaf Yellowing ◽  
Leaf Virus ◽  
Severe Leaf

Unusually severe leaf yellowing symptoms, similar to those described for yellow leaf syndrome (1), have been observed in several sugarcane clones in Guadeloupe since 1994, and since 1997 in Martinique. Leaf samples exhibiting various types of yellowing were taken from five different sugarcane clones, and analyzed by immunosorbent electron microscopy. Spherical particles, 24 to 28 nm in diameter and characteristic of luteoviruses, were found in two of five samples. The two infected samples showed yellowing on the underside of the midrib and one had a pinkish coloration on the upper side. The presence of sugarcane yellow leaf virus (ScYLV), the causal agent of sugarcane yellow leaf disease, was confirmed by reverse transcription-polymerase chain reaction (2) in these two samples and in 36 of 184 sugarcane clones bred in Guadeloupe and sent to Cirad's quarantine station in Montpellier, France. Following these observations, surveys were undertaken with a tissue blot enzyme immunoassay to analyze the distribution of ScYLV in sugarcane clones in the French West Indies. The midrib base of the first visible dewlap leaf was used to detect the presence of the virus in the phloem. In a first survey, clones of various origins worldwide were taken from germplasm collections. Two to three leaf samples per clone were analyzed from 78 clones in a collection in Guadeloupe and from 36 in a collection in Mar-tinique. Fifty of the 114 clones were infected by ScYLV, and ScYLV was detected in 21 of the 32 clones exhibiting severe leaf yellowing (score 3 or higher on a 1 to 5 scale). In a second survey, 19 leaf samples were taken from each of 53 clones from plants produced by Cirad's breeding program in Guadeloupe. The virus was detected in at least one sample for 25 of these 53 clones. ScYLV incidence in commercial fields was tested in Martinique in the variety B5992, which constitutes 57% of the cultivated area. Twenty leaves from different stools were sampled in six different fields, five of which had ScYLV-infected plants. The percentage of virus-infected stalks ranged from 0 to 90% whereas the percentage of stalks showing symptoms ranged from 50 to 100%. ScYLV appears widespread in the French West Indies, perhaps because a vector (Melanaphis sacchari) exists in Martinique and Guadeloupe. However, ScYLV was not found in all symptomatic plants, indicating that even if this luteovirus is a causal agent of leaf yellowing in the French West Indies, there may be other causal agents as well. References: (1) J. C. Comstock et al. Sugar J. 3:33, 1994. (2) J. C. Comstock et al. Sugar Cane 4:21, 1998.

Screening of Saccharum spp. genotypes for sugarcane yellow leaf virus resistance by combining symptom phenotyping and highly precise virus titration

2021 ◽  
Vol 144 ◽  
pp. 105577
Author(s):  
Roberto C.V. Burbano ◽  
Marcos C. Gonçalves ◽  
Paula M. Nobile ◽  
Ivan A. dos Anjos ◽  
Marcel F. da Silva ◽  
...  
Keyword(s):  
Virus Resistance ◽  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Virus Titration ◽  
Leaf Virus

scholarly journals Presencia del sugarcane yellow leaf virus (SCYLV) en caña de azúcar (Saccharum spp.) aislado de Colima

2018 ◽  
Vol 9 (8) ◽  
pp. 1751-1762
Author(s):  
Maria Ines Barbosa Villa ◽  
José Luis Cruz Jaramillo ◽  
Hilda Victoria Silva Rojas ◽  
Karina De la Paz García Mariscal ◽  
José Concepción García Preciado ◽  
...  
Keyword(s):  
Yellow Leaf ◽  
Rt Pcr ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Para Determinar ◽  
Leaf Virus

  La caña de azúcar es uno de los cultivos industriales importantes a nivel mundial y es afectado por diversas enfermedades virales, incluido el sugarcane yellow leaf virus (SCYLV). En México este virus se ha detectado basado en sintomatología y °Brix; sin embargo, el diagnóstico basado en estos parámetros no es concluyente. El objetivo del estudio fue detectar la presencia y distribución del SCYLV en zonas cañeras del Occidente de México (Colima, Jalisco y Nayarit) y determinar el origen filogenético de un aislado de Colima. El trabajo se desarrolló en el Campo Experimental Tecomán durante 2013-2014. Se aisló el RNA total a partir de hojas colectadas y se realizaron las RT-PCR con oligonucleótidos específicos para SCYLV. Se analizaron 233 muestras y la incidencia de plantas portadoras del SCYLV fue 14.6%, afectando a los híbridos CP 72-2086, Mex 69-290 y Atemex 96-40. El análisis BLAST mostró que la secuencia parcial CP de Colmex-317 (512 pb) es homóloga con secuencias de Brasil, China, India, Kenia y USA, además comparte porcentajes de identidad superior a 99% con otras secuencias provenientes de varios países. El análisis filogenético de Colmex-317 con secuencias parciales y genomas completos del SCYLV aislados de diversas partes del mundo reveló que el aislado mexicano pertenece al genotipo de Brasil (BRA) y se agrupó con secuencias de Brasil, China, Kenia y Sudáfrica. Sin embargo, es necesario evaluar un mayor número de aislados y secuencias de mayor longitud para determinar si el genotipo BRA es el único presente en México.

scholarly journals Variation in Infection Capacity and in Virulence Exists Between Genotypes of Sugarcane yellow leaf virus

Plant Disease ◽  
2007 ◽  
Vol 91 (3) ◽  
pp. 253-259 ◽  
Author(s):  
Youssef Abu Ahmad ◽  
Laurent Costet ◽  
Jean-Heinrich Daugrois ◽  
Samuel Nibouche ◽  
Philippe Letourmy ◽  
...  
Keyword(s):  
Yellow Leaf ◽  
Reunion Island ◽  
Melanaphis Sacchari ◽  
Sugarcane Yellow Leaf Virus ◽  
Réunion Island ◽  
Tissue Blot Immunoassay ◽  
Resistance To Infection ◽  
Leaf Virus ◽  
Leaf Symptoms

Two experiments, one in Guadeloupe and one in Réunion Island, were performed to transmit different genotypes of Sugarcane yellow leaf virus (SCYLV) to eight sugarcane cultivars differing in resistance to infection by the virus and to yellow leaf. Transmission was attempted from SCYLV-infected sugarcane plants or leaves to healthy tissue-cultured plantlets grown in vitro and with the aphid vector Melanaphis sacchari. After inoculation and elimination of insects with an insecticide, plantlets were transferred to Montpellier, France and grown in a greenhouse. Plants were tested for presence of SCYLV by tissue-blot immunoassay and reverse-transcription polymerase chain reaction after 5 to 6 months of growth. SCYLV genotypes BRA-PER, CUB, and REU were detected in 47, 62, and 39% of plants inoculated with these genotypes in Guadeloupe, respectively. SCYLV genotypes BRA-PER and REU and a mixed infection of genotypes BRA-PER and REU were detected in 56, 33, and 42% of plants inoculated with these genotypes in Réunion Island, respectively. Genotypes BRA-PER and CUB could be transmitted to all eight sugarcane cultivars, but genotype REU could never be transmitted to resistant sugarcane cvs. H78-4153 and H78-3567. SCYLV genotype REU was transmitted successfully to sugarcane cv. R570 in Guadeloupe, but not in Réunion Island. Genotypes BRA-PER and CUB induced yellow leaf symptoms in susceptible or highly susceptible sugarcane cultivars, whereas genotype REU induced very few symptoms. SCYLV was not found in several symptomatic plants, suggesting an association of disease with undetectable populations of the virus or a nonviral cause. This is the first report of variation in infection capacity and in virulence of SCYLV.

scholarly journals Molecular characterization of genetic basis of Sugarcane Yellow Leaf Virus (SCYLV) resistance in Saccharum spp. hybrid

2018 ◽  
Vol 137 (4) ◽  
pp. 598-604 ◽  
Author(s):  
Md Sariful Islam ◽  
Xiping Yang ◽  
Sushma Sood ◽  
Jack C. Comstock ◽  
Jianping Wang
Keyword(s):  
Molecular Characterization ◽  
Genetic Basis ◽  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Leaf Virus

scholarly journals Factors Affecting the Transmission and Spread of Sugarcane yellow leaf virus

Plant Disease ◽  
2000 ◽  
Vol 84 (10) ◽  
pp. 1085-1088 ◽  
Author(s):  
S. Schenck ◽  
A. T. Lehrer
Keyword(s):  
The United States ◽  
Meristem Culture ◽  
Yellow Leaf ◽  
Melanaphis Sacchari ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Factors Affecting ◽  
Sugarcane Aphid ◽  
Aphid Inoculation ◽  
Leaf Virus

Sugarcane, Saccharum spp. hybrid, is widely infected in the United States and many other countries with a yellowing and stunting disease called sugarcane yellow leaf syndrome. The causal agent, Sugarcane yellow leaf virus (ScYLV), is a Polerovirus of the Luteoviridae family. In this study, it was transmitted by the sugarcane aphid, Melanaphis sacchari, and also by the corn leaf aphid, Rhopalosiphum maidis, and the rice root aphid, R. rufiabdominalis. Two other aphids that infest sugarcane in Hawaii did not transmit the virus. Some Hawaiian sugarcane cultivars are susceptible to ScYLV, while others remain virus-free in the field. The latter were not infected when inoculated with viruliferous M. sacchari. Virus-free plants of susceptible cultivars were produced through apical meristem culture and were readily reinfected by viruliferous M. sacchari. They were also quickly reinfected when planted in a field in proximity to other infected sugarcane naturally infested with M. sacchari. Sugarcane cultivars are hybrids of several Saccharum species. In a field-grown collection of Saccharum and related species, 11 to 71% of the clones of four of the species were infected with ScYLV. None of the related genus Erianthus plants were infected, but four clones were infected experimentally by aphid inoculation. A low to moderate percentage of corn, rice, and sorghum seedlings became infected when inoculated with ScYLV, but barley, oats, and wheat proved to be very susceptible. None of seven weeds common in sugarcane fields were infected with ScYLV.

Sugarcane yellow leaf virus. [Distribution map].

2018 ◽  
Author(s):  
Keyword(s):  
Tamil Nadu ◽  
Andhra Pradesh ◽  
Uttar Pradesh ◽  
Saccharum Officinarum ◽  
Yellow Leaf ◽  
Distribution Map ◽  
Sugarcane Yellow Leaf Virus ◽  
Leaf Virus ◽  
Virus Distribution ◽  
New Distribution

Abstract A new distribution map is provided for Sugarcane yellow leaf virus. Luteoviridae: Polerovirus. Hosts: sugarcane (Saccharum officinarum), barley (Hordeum vulgare), sorghum (Sorghum bicolor). Information is given on the geographical distribution in Asia (China, Fujian, Guangdong, Guangxi, Guizhou, Hainan, Jiangxi, Yunnan, India, Andhra Pradesh, Bihar, Haryana, Karnataka, Kerala, Madhya Pradesh, Maharashtra, Punjab, Tamil Nadu, Uttar Pradesh, Uttarakhand, Indonesia, Malaysia, Philippines, Sri Lanka, Taiwan, Thailand), Africa (Egypt, Kenya, Mauritius, Reunion, South Africa, Tunisia), North America (USA, Florida, Hawaii, Louisiana, Texas), Central America & Caribbean (Barbados, Costa Rica, Cuba, Guadeloupe, Guatemala, Martinique, Nicaragua, Puerto Rico), South America (Argentina, Brazil, Colombia, Ecuador, Peru, Venezuela).

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