Screening of Saccharum spp. genotypes for sugarcane yellow leaf virus resistance by combining symptom phenotyping and highly precise virus titration

2021 ◽  
Vol 144 ◽  
pp. 105577
Author(s):  
Roberto C.V. Burbano ◽  
Marcos C. Gonçalves ◽  
Paula M. Nobile ◽  
Ivan A. dos Anjos ◽  
Marcel F. da Silva ◽  
...  
Keyword(s):  
Virus Resistance ◽  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Virus Titration ◽  
Leaf Virus

scholarly journals Genome-wide approaches for the identification of markers and genes associated with sugarcane yellow leaf virus resistance

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ricardo José Gonzaga Pimenta ◽  
Alexandre Hild Aono ◽  
Roberto Carlos Villavicencio Burbano ◽  
Alisson Esdras Coutinho ◽  
Carla Cristina da Silva ◽  
...  
Keyword(s):  
Virus Resistance ◽  
Machine Learning Algorithms ◽  
Energy Crop ◽  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Association Analyses ◽  
Sugarcane Breeding ◽  
Genetic Base ◽  
Genome Wide ◽  
Leaf Virus

AbstractSugarcane yellow leaf (SCYL), caused by the sugarcane yellow leaf virus (SCYLV) is a major disease affecting sugarcane, a leading sugar and energy crop. Despite damages caused by SCYLV, the genetic base of resistance to this virus remains largely unknown. Several methodologies have arisen to identify molecular markers associated with SCYLV resistance, which are crucial for marker-assisted selection and understanding response mechanisms to this virus. We investigated the genetic base of SCYLV resistance using dominant and codominant markers and genotypes of interest for sugarcane breeding. A sugarcane panel inoculated with SCYLV was analyzed for SCYL symptoms, and viral titer was estimated by RT-qPCR. This panel was genotyped with 662 dominant markers and 70,888 SNPs and indels with allele proportion information. We used polyploid-adapted genome-wide association analyses and machine-learning algorithms coupled with feature selection methods to establish marker-trait associations. While each approach identified unique marker sets associated with phenotypes, convergences were observed between them and demonstrated their complementarity. Lastly, we annotated these markers, identifying genes encoding emblematic participants in virus resistance mechanisms and previously unreported candidates involved in viral responses. Our approach could accelerate sugarcane breeding targeting SCYLV resistance and facilitate studies on biological processes leading to this trait.

scholarly journals Sugarcane yellow leaf virus infection leads to alterations in photosynthetic efficiency and carbohydrate accumulation in sugarcane leaves

2005 ◽  
Vol 30 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Marcos C. Gonçalves ◽  
Jorge Vega ◽  
Jurandi G. Oliveira ◽  
Mara M. A. Gomes
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Apparatus ◽  
Defense Responses ◽  
Physiological Status ◽  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Leaf Virus ◽  
Leaf Symptoms ◽  
Severe Leaf

Infection by Sugarcane yellow leaf virus (ScYLV) causes severe leaf symptoms in sugarcane (Saccharum spp.) hybrids, which indicate alterations in its photosynthetic apparatus. To gain an overview of the physiological status of infected plants, we evaluated chlorophyll a fluorescence and gas exchange assays, correlating the results with leaf metabolic surveys, i.e., photosynthetic pigments and carbohydrate contents. When compared to healthy plants, infected plants showed a reduction in potential quantum efficiency for photochemistry of photosystem (PSII) and alterations in the filling up of the plastoquinone (PQ) pool. They also showed reduction in the CO2 net exchange rates, probably as a consequence of impaired quantum yield. In addition, reductions were found in the contents of photosynthetic leaf pigments and in the ratio chlorophyll a/chlorophyll b (chla/chlb). Carbohydrate content in the leaves was increased as a secondary effect of the ScYLV infection. This article discusses the relation of virus replication and host defense responses with general alterations in the photosynthetic apparatus and in the metabolism of infected plants.

scholarly journals Presencia del sugarcane yellow leaf virus (SCYLV) en caña de azúcar (Saccharum spp.) aislado de Colima

2018 ◽  
Vol 9 (8) ◽  
pp. 1751-1762
Author(s):  
Maria Ines Barbosa Villa ◽  
José Luis Cruz Jaramillo ◽  
Hilda Victoria Silva Rojas ◽  
Karina De la Paz García Mariscal ◽  
José Concepción García Preciado ◽  
...  
Keyword(s):  
Yellow Leaf ◽  
Rt Pcr ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Para Determinar ◽  
Leaf Virus

  La caña de azúcar es uno de los cultivos industriales importantes a nivel mundial y es afectado por diversas enfermedades virales, incluido el sugarcane yellow leaf virus (SCYLV). En México este virus se ha detectado basado en sintomatología y °Brix; sin embargo, el diagnóstico basado en estos parámetros no es concluyente. El objetivo del estudio fue detectar la presencia y distribución del SCYLV en zonas cañeras del Occidente de México (Colima, Jalisco y Nayarit) y determinar el origen filogenético de un aislado de Colima. El trabajo se desarrolló en el Campo Experimental Tecomán durante 2013-2014. Se aisló el RNA total a partir de hojas colectadas y se realizaron las RT-PCR con oligonucleótidos específicos para SCYLV. Se analizaron 233 muestras y la incidencia de plantas portadoras del SCYLV fue 14.6%, afectando a los híbridos CP 72-2086, Mex 69-290 y Atemex 96-40. El análisis BLAST mostró que la secuencia parcial CP de Colmex-317 (512 pb) es homóloga con secuencias de Brasil, China, India, Kenia y USA, además comparte porcentajes de identidad superior a 99% con otras secuencias provenientes de varios países. El análisis filogenético de Colmex-317 con secuencias parciales y genomas completos del SCYLV aislados de diversas partes del mundo reveló que el aislado mexicano pertenece al genotipo de Brasil (BRA) y se agrupó con secuencias de Brasil, China, Kenia y Sudáfrica. Sin embargo, es necesario evaluar un mayor número de aislados y secuencias de mayor longitud para determinar si el genotipo BRA es el único presente en México.

Transmission of a Sugarcane yellow leaf virus resistance transgene to sexual progeny and screening by kanamycin inoculation

2010 ◽  
Vol 119 (2-3) ◽  
pp. 308-313 ◽  
Author(s):  
Neil C. Glynn ◽  
Robert A. Gilbert ◽  
Jack C. Comstock ◽  
Michael J. Davis
Keyword(s):  
Virus Resistance ◽  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Sexual Progeny ◽  
Leaf Virus

scholarly journals Molecular characterization of genetic basis of Sugarcane Yellow Leaf Virus (SCYLV) resistance in Saccharum spp. hybrid

2018 ◽  
Vol 137 (4) ◽  
pp. 598-604 ◽  
Author(s):  
Md Sariful Islam ◽  
Xiping Yang ◽  
Sushma Sood ◽  
Jack C. Comstock ◽  
Jianping Wang
Keyword(s):  
Molecular Characterization ◽  
Genetic Basis ◽  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Leaf Virus

scholarly journals Factors Affecting the Transmission and Spread of Sugarcane yellow leaf virus

Plant Disease ◽  
2000 ◽  
Vol 84 (10) ◽  
pp. 1085-1088 ◽  
Author(s):  
S. Schenck ◽  
A. T. Lehrer
Keyword(s):  
The United States ◽  
Meristem Culture ◽  
Yellow Leaf ◽  
Melanaphis Sacchari ◽  
Sugarcane Yellow Leaf Virus ◽  
Saccharum Spp ◽  
Factors Affecting ◽  
Sugarcane Aphid ◽  
Aphid Inoculation ◽  
Leaf Virus

Sugarcane, Saccharum spp. hybrid, is widely infected in the United States and many other countries with a yellowing and stunting disease called sugarcane yellow leaf syndrome. The causal agent, Sugarcane yellow leaf virus (ScYLV), is a Polerovirus of the Luteoviridae family. In this study, it was transmitted by the sugarcane aphid, Melanaphis sacchari, and also by the corn leaf aphid, Rhopalosiphum maidis, and the rice root aphid, R. rufiabdominalis. Two other aphids that infest sugarcane in Hawaii did not transmit the virus. Some Hawaiian sugarcane cultivars are susceptible to ScYLV, while others remain virus-free in the field. The latter were not infected when inoculated with viruliferous M. sacchari. Virus-free plants of susceptible cultivars were produced through apical meristem culture and were readily reinfected by viruliferous M. sacchari. They were also quickly reinfected when planted in a field in proximity to other infected sugarcane naturally infested with M. sacchari. Sugarcane cultivars are hybrids of several Saccharum species. In a field-grown collection of Saccharum and related species, 11 to 71% of the clones of four of the species were infected with ScYLV. None of the related genus Erianthus plants were infected, but four clones were infected experimentally by aphid inoculation. A low to moderate percentage of corn, rice, and sorghum seedlings became infected when inoculated with ScYLV, but barley, oats, and wheat proved to be very susceptible. None of seven weeds common in sugarcane fields were infected with ScYLV.

Sugarcane yellow leaf virus. [Distribution map].

2018 ◽  
Author(s):  
Keyword(s):  
Tamil Nadu ◽  
Andhra Pradesh ◽  
Uttar Pradesh ◽  
Saccharum Officinarum ◽  
Yellow Leaf ◽  
Distribution Map ◽  
Sugarcane Yellow Leaf Virus ◽  
Leaf Virus ◽  
Virus Distribution ◽  
New Distribution

Abstract A new distribution map is provided for Sugarcane yellow leaf virus. Luteoviridae: Polerovirus. Hosts: sugarcane (Saccharum officinarum), barley (Hordeum vulgare), sorghum (Sorghum bicolor). Information is given on the geographical distribution in Asia (China, Fujian, Guangdong, Guangxi, Guizhou, Hainan, Jiangxi, Yunnan, India, Andhra Pradesh, Bihar, Haryana, Karnataka, Kerala, Madhya Pradesh, Maharashtra, Punjab, Tamil Nadu, Uttar Pradesh, Uttarakhand, Indonesia, Malaysia, Philippines, Sri Lanka, Taiwan, Thailand), Africa (Egypt, Kenya, Mauritius, Reunion, South Africa, Tunisia), North America (USA, Florida, Hawaii, Louisiana, Texas), Central America & Caribbean (Barbados, Costa Rica, Cuba, Guadeloupe, Guatemala, Martinique, Nicaragua, Puerto Rico), South America (Argentina, Brazil, Colombia, Ecuador, Peru, Venezuela).

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