scholarly journals Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea

2005 ◽  
Vol 25 (1) ◽  
pp. 31-33
Author(s):  
Mário Paulo A. Penatti ◽  
Alex S. Silva ◽  
Geórgio F. Valadares ◽  
Domingos S. Leite
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Strong Association ◽  
Agglutination Test ◽  
Chain Reaction ◽  
Gene Encoding ◽  
Newborn Piglets ◽  
Colonization Factor ◽  
Heat Stable ◽  
Polymerase Chain

The objective of this study was to determine the presence of the colonization factor F42 in 168 strains of Escherichia coli isolated from diarrheic stools of newborn piglets. The presence of F42 in 12 (7.1%) strains was detected with the agglutination test. Through the Polymerase Chain Reaction (PCR) of F42 positive strains, gene encoding enterotoxins (ST-I, ST-II, LT-I and LT-II) were detected. The finding of ST-I/ST-II genes in 50% of the strains, ST-I (16%) and ST-II (25%) indicates a strong association of FC F42 with heat-stable enterotoxins (91%). In contrast, the thermolabile enterotoxin (LT-I and LT-II) genes were not detected. Serogroups of F42 positive strains were determined, serogroup O8 being the most prevalent (41,7%). Other serogroups, as there are O9, O11, O18, O32, O35, O98 and O101, were also identified. Thus, FC F42 was confirmed as an additional factor of virulence in the pathogenesis of porcine colibacillosis.

Rapid identification of Escherichia coli microcin J25 producing strains using polymerase chain reaction and colony blot hybridization

2001 ◽  
Vol 47 (9) ◽  
pp. 877-882 ◽  
Author(s):  
Mariela Duarte ◽  
Gilles Cottenceau ◽  
Véronique Portrait ◽  
Anne-Marie Pons
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Blot Hybridization ◽  
Rapid Identification ◽  
Molecular Techniques ◽  
Chain Reaction ◽  
Gene Encoding ◽  
Bacterial Populations ◽  
Polymerase Chain ◽  
Microcin J25

To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal contents was evaluated to detect the sequence of mcjA, the gene encoding the MccJ25 precursor. The two molecular techniques were compared with the commonly used cross-immunity tests. They generate accurate data with no obvious cross-reactions with other microcins. The results display that the producers of MccJ25 were widely distributed in the poultry intestinal habitat. The applications of these molecular methods will be useful in future studies of microcinogenic populations, and thus contribute to understand the relationships within the complex intestinal microbial ecosystem.Key words: microcin J25, microcinogenic strains detection, digoxigenin-labelled probe, colony hybridization, polymerase chain reaction.

scholarly journals Prevalence of the genes for shigella enterotoxins 1 and 2 among clinical isolates of shigella in Israel

2002 ◽  
Vol 128 (3) ◽  
pp. 533-535 ◽  
Author(s):  
M. YAVZORI ◽  
D. COHEN ◽  
N. ORR
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Vaccine Strain ◽  
Clinical Manifestation ◽  
Large Deletion ◽  
Virulence Plasmid ◽  
Chain Reaction ◽  
Gene Encoding ◽  
Non Invasive ◽  
Polymerase Chain

Two enterotoxins, shigella enterotoxin 1 (SHET1) and shigella enterotoxin 2 (SHET2) have been recently characterized and are believed to play a role in the clinical manifestation of shigellosis. One hundred and twenty-one isolates of Shigella spp. of 13 different serotypes and variants and 10 isolates of enteroinvasive Escherichia coli (EIEC) isolated in Israel, were examined by polymerase chain reaction for the presence of SHET1 and SHET2 genes. SHET1 was only prevalent among isolates of S. flexneri 2a while SHET2 was found in all the serotypes that were tested except for several isolates of S. flexneri 1b that lost their virulence plasmid during storage. In addition, we found that the S. flexneri 2a vaccine strain T-32 Istrati contains the gene encoding for SHET1 but not that encoding for SHET2, suggesting that the latter is located within a large deletion occurring in the 140 Mda plasmid of this S. flexneri 2a non-invasive vaccine strain.

Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

2019 ◽  
Vol 19 (3) ◽  
pp. 322-326 ◽  
Author(s):  
Hassan Valadbeigi ◽  
Elham Esmaeeli ◽  
Sobhan Ghafourian ◽  
Abbas Maleki ◽  
Nourkhoda Sadeghifard
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multiplex Polymerase Chain Reaction ◽  
Virulence Genes ◽  
Uropathogenic Escherichia Coli ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tract Infections

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam. Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes. Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained. Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

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