scholarly journals Outbreak of caprine abortion by Toxoplasma gondii in Midwest Brazil

2011 ◽  
Vol 31 (11) ◽  
pp. 933-937 ◽  
Author(s):  
Flávio Henrique Bravim Caldeira ◽  
Daniel Guimarães Ubiali ◽  
Isabela de Godoy ◽  
Valéria Dutra ◽  
Daniel Moura de Aguiar ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Brucella Abortus ◽  
Nested Pcr ◽  
Neospora Caninum ◽  
Pcr Assay ◽  
Serum Samples ◽  
Pathology Laboratory ◽  
Mato Grosso ◽  
Veterinary Pathology ◽  
Gross Lesions

An outbreak of abortion by Toxoplasma gondii in goats on a farm in the Brazilian Midwest is reported. Gross lesions were not observed in seven aborted fetuses submitted to the Veterinary Pathology Laboratory, Federal University of Mato Grosso, for necropsy investigation. The main histologic lesions were mononuclear cell pneumonia and necrotizing encephalitis in varying degrees of intensity. PCR for Brucella abortus and Neospora caninum and aerobic cultures were negative in all cases. Antibody titles against T. gondii varying from 1:1024 to 1:32.768 were detected in serum samples from four aborted goats. Nested-PCR assay for T. gondii were positive in brain samples of all cases submitted. These findings indicate that T. gondii infection should be considered in the diagnosis of abortion in goats in Midwest Brazil.

scholarly journals Detection of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii in horses, dogs and cat

2019 ◽  
Vol 56 (2) ◽  
pp. e152918
Author(s):  
Marilia De Oliveira Koch ◽  
Luciane Maria Laskoski ◽  
Daniel Moura de Aguiar ◽  
Bianca Ressetti da Silva ◽  
Reinaldo Ramos Régio ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Clinical Pathology ◽  
Antibody Test ◽  
Serum Samples ◽  
Pathology Laboratory ◽  
Sarcocystis Neurona ◽  
Neurological Signs ◽  
The University

The occurrence and distribution of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii was investigated in horses, dogs and cats from Curitiba, Paraná state, Brazil. Serum samples were selected from 100 horses, 100 dogs and 100 cats from the routine of the Veterinary Clinical Pathology Laboratory in the Veterinary Hospital of the University of Paraná (UFPR). The 100 dog samples were divided into two groups: 35 samples from dogs with neurological sign (convulsion) and 65 samples from dogs without neurological signs. The animals were adults of different breeds, males and females. Samples were analyzed by indirect fluorescent antibody test (IFAT) for protozoa S. neurona, N. caninum andT. gondii at the following cut-off dilutions: horses: 1:50, 1:50 and 1:16; dogs: 1:50, 1:50 and 1:16; cats: 1:50, 1:50 and 1:50, respectively. The obtained results were 42% of horses, 7% of dogs and 5% of cats seropositive for S. neurona; 58% of horses, 68% of dogs and 42% of cats seropositive to N. caninum, and 36% of horses, 20% of dogs and 21% of cats seropositive for T. gondii. Among the dogs with neurological signs, 8.6%, 68.6% and 25.7% were seropositive for S. neurona, N. caninum and T. gondii, respectively. Among the dogs without neurological signs, 6.2% 67.7% and 16.9% were seropositive for S. neurona, N. caninum and T. gondii, respectively. No statistical difference was found between groups of seropositive dogs for the three protozoa with neurological signs and without neurological signs. Co-infection and high antibody titers were detected. The antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii were found widely distributed among horses, dogs and cats in the region of Curitiba, state of Paraná, Brazil.   

scholarly journals Seroprevalence of Toxoplasma gondii, Neospora caninum, and Leishmania spp. in hunting dogs from Mato Grosso do Sul, Brazil

2021 ◽  
Vol 51 (5) ◽  
Author(s):  
Silvana Marques Caramalac ◽  
Simone Marques Caramalac ◽  
Pablo Menegon Castilho ◽  
Juliana Izidoro Lucas ◽  
Ana Flávia Minutti ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rural Area ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Parasitic Infections ◽  
Serum Samples ◽  
Mato Grosso ◽  
Hunting Dogs ◽  
Leishmania Spp ◽  
Mato Grosso Do Sul

ABSTRACT: Toxoplasmosis, neosporosis, and leishmaniasis are important diseases of worldwide distribution and can affect both pets and humans. Hunting dogs have been trained to hunt domestic and wild animals, which makes them more exposed to parasitic infections. The present study aimed to evaluate the seroprevalence of Toxoplasma gondii, Neospora caninum, and Leishmania spp. in hunting dogs from a rural area in Mato Grosso do Sul, Brazil. Serum samples were collected from 39 American Foxhound dogs, and the sex and age variables were recorded. Serum samples were subjected to an indirect fluorescent antibody test (IFAT) to detect antibodies. Seroprevalence was 35.9%, 15.4%, and 2.6% for Toxoplasma gondii, Neospora caninum e Leishmania spp., respectively. There was no statistical difference between genders for these diseases (P>0.05). Results demonstrated a circulation of these protozoa in hunting dogs in a rural area of the state of Mato Grosso do Sul, which can contribute to the epidemiology of these diseases.

scholarly journals Antibodies anti-Toxoplasma gondii and anti-Neospora caninum in backyard pigs from the state of Mato Grosso, Brazil

2019 ◽  
Vol 28 (3) ◽  
pp. 403-409 ◽  
Author(s):  
Make Kawatake Minetto ◽  
Rute Witter ◽  
Anderson Castro Soares de Oliveira ◽  
João Augusto Minetto ◽  
Marcelo Luís Barros ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Serum Samples ◽  
Mato Grosso ◽  
Pig Farming ◽  
Indirect Immunofluorescent Assay ◽  
Level Model ◽  
The Impact ◽  
Indirect Immunofluorescent

Abstract To estimate the seroprevalence of Toxoplasma gondii and Neospora caninum, using an indirect immunofluorescent assay (IFA), and identify the risk factors associated, serum samples were collected from 1,070 pigs from 320 backyard pig farming in the of Mato Grosso state. The animal-level seroprevalence of T. gondii and N. caninum was 32.48% and 13.49%, respectively, with a herd seroprevalence of 55.63% for T. gondii and 27.81% for N. caninum. Feeding the animals with leftovers increases the probability of the presence of anti-T. gondii antibodies in pigs by 1.09-fold. Unlike to T. gondii, feeding with leftovers was found to be negatively associated with N. caninum seropositivity in farm-level analysis and in the animal-level model, so decreasing the chances of positivity. Yet, age was considered a risk factor for N. caninum seropositivity. Further studies are necessary to evaluate the impact of T. gondii infection on backyard pig farming production, and its importance as a source of toxoplasmosis infection in humans in the Mato Grosso state, as well as, the role of domestic pigs in the epidemiology of neosporosis.

scholarly journals Serological detection of Toxoplasma gondii, Leishmania infantum and Neospora caninum in cats from an area endemic for leishmaniasis in Brazil

2014 ◽  
Vol 23 (4) ◽  
pp. 449-455 ◽  
Author(s):  
Keyla Carstens Marques de Sousa ◽  
Heitor Miraglia Herrera ◽  
Iara Helena Domingos ◽  
João Bosco Vilela Campos ◽  
Isabela Maria Campanelli dos Santos ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Leishmania Infantum ◽  
Significant Interaction ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Domestic Cats ◽  
Serum Samples ◽  
Mato Grosso ◽  
Mato Grosso Do Sul

An investigation was made into the occurrence of antibodies to Toxoplasma gondii, Leishmania infantum and Neospora caninum in 151 domestic cats, based on the indirect fluorescent antibody test (IFAT). Serum samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats; 55 males and 96 females) in Campo Grande, Mato Grosso do Sul, Brazil between January and April 2013. IgG antibodies to T. gondii, L. infantum and N. caninum were found, respectively, in 49 (32.5%), 34 (22.5%) and 10 (6.6%) sampled cats. A positive correlation was found between T. gondii and N. caninum, T. gondii and L. infantum, and N. caninum and L. infantum (p <0.05) infections. Also, a significant interaction was identified between gender and area of activity on the probability of T. gondii (p = 0.0324) infection. However, no significant interaction was observed between gender and area of activity on infections by either N. caninum or L. infantum. This study showed that cats from an area endemic for visceral leishmaniasis in Brazil are exposed to three different protozoans, two of which are causal agents of important zoonosis.

scholarly journals Presence of antibodies against Toxoplasma gondii, Neospora caninum and Leishmania infantum in dogs from Piauí

2011 ◽  
Vol 20 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Marcos Gomes Lopes ◽  
Ivete Lopes Mendonça ◽  
Kleber Portela Fortes ◽  
Marcos Amaku ◽  
Hilda de Fátima Jesus Pena ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Leishmania Infantum ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Northeastern Brazil ◽  
Leishmania Infection ◽  
Serum Samples ◽  
Chi Square ◽  
Leishmania Spp ◽  
And Gender

This study aimed to evaluate the presence of antibodies against Neospora caninum, Toxoplasma gondii and Leishmania infantum in dogs attended at the Veterinary Hospital of the Federal University of Piauí, Northeastern Brazil, where there are no reports of the occurrence of N. caninum and T. gondii in dogs. Serum samples from 530 dogs of genders, different ages and breeds from the municipality of Teresina and nearby towns were analyzed using three indirect fluorescent antibody tests, each one targeting one of the three agents. The associations between the parasites and gender, breed and age of the dogs were assessed by the chi-square test (p > 0.05). The occurrence of antibodies to N. caninum, T. gondii and L. infantum was 3.2, 18.0 and 78.1%, respectively. Toxoplasma gondii was more frequently found in older dogs (p < 0.05) whereas L. infantum was more common in animals aged between 1 to 3 years (p < 0.05). In order to evaluate potential associations between the presence of anti-N. caninum and anti-T. gondii antibodies and Leishmania infection, 240 dogs were selected (120 positive and 120 negative for Leishmania spp.), based on serological and parasitological diagnoses. No association was found between Leishmania spp. and the coccidian parasites (p > 0.05). The results confirm the exposure of dogs to these parasites in the State of Piauí.

scholarly journals Seroprevalence of Toxoplasma gondii infection in swine matrices in Nova Mutum and Diamantino, Mato Grosso, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 254-255 ◽  
Author(s):  
Lívia Saab Muraro ◽  
João Garcia Caramori Júnior ◽  
Maria Regina Reis Amendoeira ◽  
Joyce Alves Pereira ◽  
João Xavier de Oliveira Filho ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
The State ◽  
Indirect Fluorescent Antibody ◽  
Risk Of Infection ◽  
Serum Samples ◽  
Mato Grosso ◽  
Toxoplasma Gondii Infection

This report aimed to assess the seroprevalence of Toxoplasma gondii infection in 708 swine matrices in Nova Mutum and Diamantino in the state of Mato Grosso, Central-West Brazil. Serum samples were examined by indirect fluorescent antibody test (IFAT). It was found a seroprevalence of 12.8%, considering titers >64. Therefore, the data reinforce the need for appropriate management of swine raising to minimize the risk of infection of pigs with T. gondii.

scholarly journals Exposure of free-living jaguars to Toxoplasma gondii, Neospora caninum and Sarcocystis neurona in the Brazilian Pantanal

2014 ◽  
Vol 23 (4) ◽  
pp. 547-553 ◽  
Author(s):  
Selma Samiko Miyazaki Onuma ◽  
Andréia Lima Tomé Melo ◽  
Daniel Luis Zanella Kantek ◽  
Peter Gransden Crawshaw-Junior ◽  
Ronaldo Gonçalves Morato ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Free Living ◽  
Sarcocystis Neurona ◽  
Mato Grosso ◽  
Apicomplexan Parasites ◽  
Wide Range ◽  
Coccidian Parasites ◽  
Brazilian Pantanal

Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are related apicomplexan parasites that cause reproductive and neurological disorders in a wide range of domestic and wild animals. In the present study, the immunofluorescence antibody test (IFAT) was used to investigate the presence of antibodies against T. gondii, N. caninum and S. neurona in the sera of 11 free-living jaguars (Panthera onca) in two protected areas in the Pantanal region of Mato Grosso state, Brazil. Ten jaguars (90.9%) showed seropositivity for T. gondii, eight (72.7%) for S. neurona, and seven (63.6%) for N. caninum antigens. Our findings reveal exposure of jaguars to these related coccidian parasites and circulation of these pathogens in this wild ecosystem. To the best of our knowledge, this is the first serological detection of N. caninum and S. neurona in free-living jaguars.

scholarly journals Detection of Toxoplasma gondii in Diabetic Patients Using the Nested PCR Assay via RE and B1 Genes

2016 ◽  
Vol 9 (2) ◽  
Author(s):  
Mohammad Mousavi ◽  
Ramin Saravani ◽  
Mohammad Jafari Modrek ◽  
Mahnaz Shahrakipour ◽  
Sina Sekandarpour
Keyword(s):  
Toxoplasma Gondii ◽  
Nested Pcr ◽  
Diabetic Patients ◽  
Pcr Assay

scholarly journals Serological evaluation of Neospora caninum in pregnant women treated at referral center for prenatal screening in Mato Grosso do Sul, Brazil

2020 ◽  
Vol 29 (4) ◽  
Author(s):  
Pâmella Oliveira Duarte ◽  
Bárbara Guimarães Csordas ◽  
Leandra Marla Oshiro ◽  
Leandro de Oliveira Souza Higa ◽  
Namor Pinheiro Zimmermann ◽  
...  
Keyword(s):  
Western Blot ◽  
Pregnant Women ◽  
Canis Lupus ◽  
Prenatal Screening ◽  
Neospora Caninum ◽  
Serum Samples ◽  
Recent Infection ◽  
Mato Grosso ◽  
Referral Center ◽  
Mato Grosso Do Sul

Abstract Neospora caninum is an obligate intracellular protozoan with canids (Canis domesticus, Canis lupus dingo, Canis latrans, Canis lupus) as its definitive hosts. The objective of this study was to detect anti-N. caninum antibodies in pregnant women seen at referral center for prenatal screening in the state of state Mato Grosso do Sul, Brazil. A total of 188 serum samples from pregnant women provided by the Instituto de Pesquisa, Ensino e Diagnósticos da APAE de Campo Grande (IPED/ APAE) were subjected to IFA test and western blot analysis. The samples were divided into three groups: 23/99 samples from the seropositive group for toxoplasmosis were positive for anti-N. caninum IgG antibodies, and 9/99 positive for IgM; in the HIV group, 7/33 were positive for IgG; and in the HIV+toxoplasmosis group, 13/56 were positive for IgG and two positive for IgM. The seropositivity for IgG was assessed by western blot by testing 43 IFA test positive samples using rNcSRS2 (Nc-p43) as antigen. The serological results of the present study suggest that exposure of these pregnant women to the parasite N. caninum and presence of IgM antibodies are indicative of recent infection. Further studies are needed to establish the possibility of active infection.

scholarly journals Toxoplasma gondii and Neospora caninum infections and factors associated in goats in the Parana state, Southern Brazil

2020 ◽  
Vol 29 (4) ◽  
Author(s):  
Paulo Roberto Romanelli ◽  
Andressa Maria Rorato Nascimento de Matos ◽  
Fernanda Pinto-Ferreira ◽  
Eloiza Teles Caldart ◽  
Juliana Silva de Oliveira ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Regression Models ◽  
Production Management ◽  
Indirect Elisa ◽  
Neospora Caninum ◽  
Serum Samples ◽  
Factors Associated ◽  
Reproductive Management ◽  
Multiple Regression Models ◽  
South Brazil

Abstract The aim of this study was to determine the occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies and their associated factors among goats in farms from the Paraná state, South Brazil. The serological analysis was performed by indirect ELISA on 629 goat serum samples collected from 32 farms distributed in five mesorregions from the state. Seropositivity was observed in 30.7% of the animals for T. gondii, 6.3% for N. caninum, and 3.0% for both agents. Final multiple regression models showed that the use of assisted reproduction decreased the chance of seropositivity for T. gondii (PR=0.70) and the slaughtering site on the farm (PR=2.03) increased it. To N. caninum, the supplying concentrated feed to animals (OR=4.80) and animal pre-weaning death (OR=9.96) increased the chance of seropositivity. The variables associated with these seropositivities suggest deficiencies in sanitation and reproductive management. In addition, it recognizes critical points of infection for animal production management.

Sign in / Sign up

Export Citation Format

Share Document