Uterine lymphangiography: comparison of two methods for locating the medial iliac lymph node

Different methods for lymphatic mapping in dogs, such as infusing tissues with vital dyes or radioactive substances, have been studied, aiming at the early detection of lymph node metastasis. Thus, one could anticipate therapeutic measures and, consequently, prolong the survival and improve the quality of life of the patients. The objectives of this experiment were to locate the nodes responsible for draining the uterine body and horns and to try to establish the relationship between the uterus and the medial iliac lymph nodes to contribute to the early diagnosis and prognosis of uterine disorders. We studied 15 female dogs divided into two groups (5 dead and 10 intraoperative ovariohysterectomy bitches). The dye used was patent blue V (Patent Bleu V®). It was observed that the iliac lymph node chain receives much of the uterine (horns) drainage. This method should be considered for safer studies of uterine sanity. This information suggests that evaluating these lymph nodes will allow correlating changes in their physiological status with uterine pathologies.

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Blue Skin Rash And Swelling Of The Orbits After Injection Of Patent Blue V Dye During Sentinel Lymph Node Biopsy - A Case Report

Polish Journal of Surgery ◽

10.2478/v10035-010-0085-y ◽

2010 ◽

Vol 82 (10) ◽

Author(s):

Łukasz Strzępek ◽

Wojciech Wysocki ◽

Piotr Skotnicki ◽

Jerzy Mituś

Keyword(s):

Case Report ◽

Lymph Node ◽

Sentinel Lymph Node ◽

Sentinel Lymph Node Biopsy ◽

Skin Rash ◽

Lymph Node Biopsy ◽

Patent Blue ◽

Node Biopsy ◽

Patent Blue V

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D3 LYMPHADENECTOMY IN RIGHT COLONIC RESECTION

JMS SKIMS ◽

10.33883/jms.v21i2.345 ◽

2019 ◽

Vol 21 (2) ◽

pp. 109-113

Author(s):

Arshad Ahmed Baba ◽

Zahid Kaleem ◽

Fazl Qadir Parray ◽

Nisar Ahmad Chowdri ◽

Rouf Ahmad Wani ◽

...

Keyword(s):

Lymph Node ◽

Lymph Nodes ◽

Lymph Node Dissection ◽

Node Dissection ◽

D3 Lymphadenectomy ◽

Quality Of Surgery ◽

Staining Techniques ◽

Node Metastases ◽

The Individual

Lymph node dissection in colon cancer is without a doubt necessary, it is just the extent of that dissection that is still under debate. As the individual steps of an oncologic operation cannot be separated from each other, analysis of the significance of lymph node dissection alone is difficult. It has been proven that the T category is directly related to the number and central spread of lymph node metastases. Micrometastases and isolated tumor cells may be detected in lymph nodes by using special staining techniques; their presence may worsen prognosis significantly and approximate it to UICC stage III. The numbers of dissected lymph nodes and the ratio of involved versus dissected lymph nodes have been used as markers for quality of surgery and histopathological evaluation. JMS 2018: 21 (2):109-113

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Sentinel Lymphadenectomy in Vulvar Cancer Using Near-Infrared Fluorescence From Indocyanine Green Compared With Technetium 99m Nanocolloid

International Journal of Gynecological Cancer ◽

10.1097/igc.0000000000000996 ◽

2017 ◽

Vol 27 (4) ◽

pp. 805-812 ◽

Cited By ~ 14

Author(s):

Philipp Soergel ◽

Hermann Hertel ◽

Anna Kaarina Nacke ◽

Rüdiger Klapdor ◽

Thorsten Derlin ◽

...

Keyword(s):

Lymph Node ◽

Confidence Interval ◽

Lymph Nodes ◽

Indocyanine Green ◽

Near Infrared ◽

Vulvar Cancer ◽

Sentinel Lymph Nodes ◽

Infrared Band ◽

Green Is ◽

Patent Blue

ObjectiveNowadays, sentinel diagnostic is performed using technetium 99m (99mTc) nanocolloid as a radioactive marker and sometimes patent blue. In the last years, indocyanine green has been evaluated for sentinel diagnostic in different tumor entities. Indocyanine green is a fluorescent molecule that emits a light signal in the near-infrared band after excitation. Our study aimed to evaluate indocyanine green compared with the criterion-standard99mTc-nanocolloid.MethodsWe included patients with primary, unifocal vulvar cancer of less than 4 cm with clinically node-negative groins in this prospective trial. Sentinel diagnostic was carried out using99mTc-nanocolloid, indocyanine green, and patent blue. We examined each groin for light signals from the near-infrared band, for radioactivity, and for blue staining. A sentinel lymph node was defined as a99mTc-nanocolloid–positive lymph node. All sentinel lymph nodes and all additional blue or fluorescent lymph nodes were excised and tested and then sent for histologic examination.ResultsIn all, 27 patients were included in whom we found 91 sentinel lymph nodes in 52 groins. All these lymph nodes were positive for indocyanine green, also giving a sensitivity of 100% (95% confidence interval [CI], 96.0%–100%) compared with99mTc-nanocolloid. Eight additional lymph nodes showed indocyanine green fluorescence but no99mTc positivity, so that the positive predictive value was 91.9% (95% confidence interval, 84.6%–96.5%). In 1 patient, a false-negative sentinel missed by all 3 modalities was found.ConclusionsOur results show that indocyanine green is a promising approach for inguinal sentinel identification in vulvar cancer with a similar sensitivity as radioactive99mTc-nanocolloid and worth to be evaluated in further studies.

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Lymph-migrating, tissue-derived dendritic cells are minor constituents within steady-state lymph nodes

Journal of Experimental Medicine ◽

10.1084/jem.20081430 ◽

2008 ◽

Vol 205 (12) ◽

pp. 2839-2850 ◽

Cited By ~ 160

Author(s):

Claudia Jakubzick ◽

Milena Bogunovic ◽

Anthony J. Bonito ◽

Emma L. Kuan ◽

Miriam Merad ◽

...

Keyword(s):

Dendritic Cells ◽

Lymph Node ◽

Steady State ◽

Lymph Nodes ◽

Lymphatic Vessels ◽

Inflammatory Stimulus ◽

Major Fraction ◽

Reporter Mouse ◽

Relationship Of ◽

The Relationship

Observations that dendritic cells (DCs) constitutively enter afferent lymphatic vessels in many organs and that DCs in some tissues, such as the lung, turnover rapidly in the steady state have led to the concept that a major fraction of lymph node DCs are derived from migratory DCs that enter the lymph node through upstream afferent lymphatic vessels. We used the lysozyme M–Cre reporter mouse strain to assess the relationship of lymph node and nonlymphoid organ DCs. Our findings challenge the idea that a substantial proportion of lymph node DCs derive from the upstream tissue during homeostasis. Instead, our analysis suggests that nonlymphoid organ DCs comprise a major population of DCs within lymph nodes only after introduction of an inflammatory stimulus.

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Adverse reactions to Patent Blue V dye used in sentinel lymph node biopsy for Melanoma

European Journal of Plastic Surgery ◽

10.1007/s00238-010-0515-4 ◽

2010 ◽

Vol 34 (3) ◽

pp. 201-204 ◽

Cited By ~ 3

Author(s):

Milap G. Rughani ◽

Marc C. Swan ◽

Titus S. Adams ◽

Mark R. Middleton ◽

Oliver C. Cassell

Keyword(s):

Lymph Node ◽

Sentinel Lymph Node ◽

Sentinel Lymph Node Biopsy ◽

Adverse Reactions ◽

Lymph Node Biopsy ◽

Patent Blue ◽

Node Biopsy ◽

Patent Blue V

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RT-PCR and immunohistochemical evaluation of sentinel lymph nodes after in-vivo mapping with Patent Blue V in colon cancer patients

European Journal of Gastroenterology & Hepatology ◽

10.1097/00042737-200601000-00140 ◽

2006 ◽

Vol 18 (1) ◽

pp. A41

Author(s):

W Kelder ◽

A van den Berg ◽

ATMG Tiebosch ◽

PC Baas ◽

JTM Plukker

Keyword(s):

Colon Cancer ◽

Lymph Nodes ◽

Cancer Patients ◽

Sentinel Lymph Nodes ◽

Rt Pcr ◽

Patent Blue ◽

Patent Blue V

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Differential transcriptomics in sarcoidosis lung and lymph node granulomas with comparisons to pathogen-specific granulomas

10.21203/rs.3.rs-29265/v2 ◽

2020 ◽

Author(s):

Nancy G. Casanova ◽

Manuel L Gonzalez-Garay ◽

Belinda Sun ◽

Christian Bime ◽

Kenneth S. Knox ◽

...

Keyword(s):

Gene Expression ◽

Lymph Node ◽

Lymph Nodes ◽

Immunological Response ◽

Functional Enrichment ◽

Genomic Biomarkers ◽

Diagnosis And Prognosis ◽

Genomic Markers ◽

Affected Tissue ◽

Granulomatous Diseases

Abstract Rationale: Despite the availability of multi-“omics” strategies, insights into the etiology and pathogenesis of sarcoidosis have been elusive. This is partly due to the lack of reliable preclinical models and a paucity of validated biomarkers. As granulomas are a key feature of sarcoidosis, we speculate that direct genomic interrogation of sarcoid tissues, may lead to identification of dysregulated gene pathways or biomarker signatures. Objective: To facilitate the development sarcoidosis genomic biomarkers by gene expression profiling of sarcoidosis granulomas in lung and lymph node tissues (most commonly affected organs) and comparison to infectious granulomas (coccidiodomycosis and tuberculosis). Methods: Transcriptomic profiles of immune-related gene from micro-dissected lungs and mediastinal lymph nodes sarcoidosis granulomas was compared to infectious granulomas. Differentially-expressed genes (DEGs) were profiled, compared among the three granulomatous diseases and analyzed for functional enrichment pathways. Results: Despite histologic similarities, DEGs and pathway enrichment markedly differed in sarcoidosis granulomas from lymph nodes and lung. Lymph nodes showed a clear immunological response, whereas a structural regenerative response was observed in lung. Sarcoidosis granuloma gene expression data corroborated previously reported genomic biomarkers, excluded others and identified new genomic markers present in lung and lymph nodes, ADAMTS1, CXCL2, FABP4 . Comparisons between sarcoidosis and pathogen granulomas identified pathway divergences and commonalities at gene expression level. Conclusion : These findings suggest the importance of tissue and disease-specificity evaluation when exploring sarcoidosis genomic markers. This relevant translational information in two commonly affected tissue in sarcoidosis and other two histopathological similar infections provides meaningful specific genomic-derived biomarkers for sarcoidosis diagnosis and prognosis.

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Differential transcriptomics in sarcoidosis lung and lymph node granulomas with comparisons to pathogen-specific granulomas

10.21203/rs.3.rs-29265/v1 ◽

2020 ◽

Author(s):

Nancy G. Casanova ◽

Manuel L Gonzalez-Garay ◽

Belinda Sun ◽

Christian Bime ◽

Kenneth S. Knox ◽

...

Keyword(s):

Gene Expression ◽

Lymph Node ◽

Lymph Nodes ◽

Immunological Response ◽

Functional Enrichment ◽

Genomic Biomarkers ◽

Diagnosis And Prognosis ◽

Genomic Markers ◽

Affected Tissue ◽

Granulomatous Diseases

Abstract Rationale: Despite the availability of multi-“omics” strategies, insights into the etiology and pathogenesis of sarcoidosis have been elusive. This is partly due to the lack of reliable preclinical models and a paucity of validated biomarkers. As granulomas are a key feature of sarcoidosis, we speculate that direct genomic interrogation of sarcoid tissues, may lead to identification of dysregulated gene pathways or biomarker signatures.Objective: To facilitate the development sarcoidosis genomic biomarkers by gene expression profiling of sarcoidosis granulomas in lung and lymph node tissues (most commonly affected organs) and comparison to infectious granulomas (coccidiodomycosis and tuberculosis). Methods: Transcriptomic profiles of immune-related gene from micro-dissected lungs and mediastinal lymph nodes sarcoidosis granulomas was compared to infectious granulomas. Differentially-expressed genes (DEGs) were profiled, compared among the three granulomatous diseases and analyzed for functional enrichment pathways. Results: Despite histologic similarities, DEGs and pathway enrichment markedly differed in sarcoidosis granulomas from lymph nodes and lung. Lymph nodes showed a clear immunological response, whereas a structural regenerative response was observed in lung. Sarcoidosis granuloma gene expression data corroborated previously reported genomic biomarkers, excluded others and identified new genomic markers present in lung and lymph nodes, ADAMTS1, CXCL2, FABP4. Comparisons between sarcoidosis and pathogen granulomas identified pathway divergences and commonalities at gene expression level.Conclusion: These findings suggest the importance of tissue and disease-specificity evaluation when exploring sarcoidosis genomic markers. This relevant translational information in two commonly affected tissue in sarcoidosis and other two histopathological similar infections provides meaningful specific genomic-derived biomarkers for sarcoidosis diagnosis and prognosis.

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