scholarly journals A method to estimate the damage of Diabrotica speciosa larvae on potato tubers

2021 ◽  
Vol 39 (3) ◽  
pp. 245-249
Author(s):  
Marcos Roberto Barboza ◽  
Vitor Hugo Outeiro ◽  
Alessandra Tokarski ◽  
Caroline Rech ◽  
Jackson Kawakami ◽  
...  

ABSTRACT The marketable value of potato tubers is affected by damage caused by Diabrotica speciosa, whose larvae create holes on the tubers’ skin and internal feeding tunnels. The estimation of potato tuber damage is usually performed by assessing the external damage to the detriment of feeding tunnels caused by larvae. Thus, we propose a method to estimate the damage caused by D. speciosa larvae, considering the external and internal aspects of the tubers separately. For that, potato plants cv. Agata were grown under different infestations of larvae, measuring the area occupied by the holes and the volume of internal feeding tunnels, relating these data to the total area and volume of the tuber. The methodology used allowed us to characterize an increase in damage in tubers with the highest infestation of larvae. The correlation between internal and external damage caused by D. speciosa larvae was not significant, indicating that external damage alone is not an adequate parameter for the diagnosis of overall tuber quality. However, the method proposed here provides information regarding the volume of pulp consumed by the larvae, the extent of the galleries formed, and the relative volume of damage concerning the total tuber. Moreover, the method proposed here contemplates a more precise analysis of the external area damaged by herbivory relative to the total area of the tuber, which is not commonly considered in studies of underground plant structures.

2019 ◽  
Author(s):  
hennypuspitasari ◽  
Warnita

Research on the growth and formation of potato tubers was conducted to determine the effect of rhizobacteria isolates and coumarin applications growth inhibitors. Bacteria are isolated from the roots of potato plants that grow in the healthy roots of potato plants.The study used a two-factor complete randomized design with three replications. The first factor was rhizobacterial isolates BT.4.1 isolates, BT.4.2 isolates, BT.4.3 isolates. The second factor was the concentration of coumarin 0, 50, 100 and 150 mg L-1. Thus from the results of this study obtained of rhizobacteria BT.4.1, BT.4.3 and coumarin 100 - 150 mg L-1 increases the growth and yield of potato crops. Based on the results of potato tuber produced, the combination of BT.4.1 rhizobacteria, and coumarin 150 mg L-1 can increase growth and yield.


2019 ◽  
Author(s):  
hennypuspitasari

Research on the growth and formation of potato tubers was conducted to determine the effect of rhizobacteria isolates and coumarin applications growth inhibitors. Bacteria are isolated from the roots of potato plants that grow in the healthy roots of potato plants.The study used a two-factor complete randomized design with three replications. The first factor was rhizobacterial isolates BT.4.1 isolates, BT.4.2 isolates, BT.4.3 isolates. The second factor was the concentration of coumarin 0, 50, 100 and 150 mg L-1. Thus from the results of this study obtained of rhizobacteria BT.4.1, BT.4.3 and coumarin 100 - 150 mg L-1 increases the growth and yield of potato crops. Based on the results of potato tuber produced, the combination of BT.4.1 rhizobacteria, and coumarin 150 mg L-1 can increase growth and yield.


1986 ◽  
Vol 26 (6) ◽  
pp. 745 ◽  
Author(s):  
PA Taylor ◽  
SP Flett ◽  
RFde Boer ◽  
D Marshall

The period of susceptibility of potato tubers to powdery scab (Spongospora subterranea) was studied by inoculating potato plants with spores, or by watering plants in infested soil, at different stages of plant development in greenhouse conditions. Maximum susceptibility began about 1 week before the stage when 50% of stolons had swollen to at least 5-mm diameter (tuber set), and ended 3-4 weeks later. With holding irrigation water during this period reduced the severity of powdery scab by 65-75% in field experiments in 1981-82 and 1982-83, but had no apparent effect on disease severity in 3 out of 6 large-scale field trials during 1984-85.


2012 ◽  
Vol 158 (3) ◽  
pp. 1306-1318 ◽  
Author(s):  
Natalia Carreno-Quintero ◽  
Animesh Acharjee ◽  
Chris Maliepaard ◽  
Christian W.B. Bachem ◽  
Roland Mumm ◽  
...  

2008 ◽  
Vol 44 (2) ◽  
pp. 157-166 ◽  
Author(s):  
S. M. HARPER ◽  
F. P. C. BLAMEY

SUMMARYInternal browning disorders, including brown fleck (BF), in potato (Solanum tuberosum) tubers greatly reduce tuber quality, but the causes are not well understood. This is due, in part, to the highly variable data provided by visual value-based rating systems. A digital imaging technique was developed to quantify accurately the incidence of internal browning in potato tubers. Images of tuber sections were scanned using a flatbed scanner and digitally enhanced to highlight tuber BF lesions, and the area of affected tissue calculated using pixel quantification software. Digital imaging allowed for the determination of previously unused indices of the incidence and severity of internal browning in potato tubers. Statistical analysis of the comparison between digitally derived and visual-rating BF data from a glasshouse experiment showed that digital data greatly improved the delineation of treatment effects. The F-test probability was further improved through square root or logarithmic data transformations of the digital data, but not of the visual-rating data. Data from a field experiment showed that the area of tuber affected by BF and the number of small BF lesions increased with time and with increase in tuber size. The results from this study indicate that digital imaging of internal browning disorders of potato tubers holds much promise in determining their causes that heretofore have proved elusive.


Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 734 ◽  
Author(s):  
Bruce R. Robinson ◽  
Carolina Garcia Salinas ◽  
Perla Ramos Parra ◽  
John Bamberg ◽  
Rocio I. Diaz de la Garza ◽  
...  

Biofortification of folates in staple crops is an important strategy to help eradicate human folate deficiencies. Folate biofortification using genetic engineering has shown great success in rice grain, tomato fruit, lettuce, and potato tuber. However, consumers’ skepticism, juridical hurdles, and lack of economic model have prevented the widespread adoption of nutritionally-enhanced genetically-engineered (GE) food crops. Meanwhile, little effort has been made to biofortify food crops with folate by breeding. Previously we reported >10-fold variation in folate content in potato genotypes. To facilitate breeding for enhanced folate content, we attempted to identify genes that control folate content in potato tuber. For this, we analyzed the expression of folate biosynthesis and salvage genes in low- and high-folate potato genotypes. First, RNA-Seq analysis showed that, amongst all folate biosynthesis and salvage genes analyzed, only one gene, which encodes γ-glutamyl hydrolase 1 (GGH1), was consistently expressed at higher levels in high- compared to low-folate segregants of a Solanum boliviense Dunal accession. Second, quantitative PCR showed that GGH1 transcript levels were higher in high- compared to low-folate segregants for seven out of eight pairs of folate segregants analyzed. These results suggest that GGH1 gene expression is an indicator of folate content in potato tubers.


1945 ◽  
Vol 23c (2) ◽  
pp. 76-78 ◽  
Author(s):  
W. Newton ◽  
W. Jones

Freshly cut sections of frozen potato tuber and apple fruit tissue exhibit a brilliant fluorescence when examined in a dark room under an ultra-violet (Stroblite) lamp. The fluorescence disappears from the apple tissue upon thawing but is retained in potato tuber tissue. Thus the ultra-violet lamp serves as a useful diagnostic means of detecting frost or low temperature injury in potato tubers. Many other plant tissues were frozen and examined, but proved to be non-fluorescent. Acetone extracts of both normal and frozen potato tuber tissue were fluorescent, but similar extracts of both frozen and normal apple fruit were not. Although normal potato tissue is non-fluorescent and frozen tissue is brightly fluorescent, no differences were found in the brightness of the acetone extracts.


Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1292-1301 ◽  
Author(s):  
M. I. Siri ◽  
A. Sanabria ◽  
M. J. Pianzzola

Bacterial wilt, caused by Ralstonia solanacearum, is a major disease affecting potato (Solanum tuberosum) production worldwide. Although local reports suggest that the disease is widespread in Uruguay, characterization of prevalent R. solanacearum strains in that country has not been done. In all, 28 strains of R. solanacearum isolated from major potato-growing areas in Uruguay were evaluated, including 26 strains isolated from potato tubers and 2 from soil samples. All strains belonged to phylotype IIB, sequevar 1 (race 3, biovar 2). Genetic diversity of strains was assessed by repetitive-sequence polymerase chain reaction, which showed that the Uruguayan strains constituted a homogeneous group. In contrast, inoculation of the strains on tomato and potato plants showed, for the first time, different levels of aggressiveness among R. solanacearum strains belonging to phylotype IIB, sequevar 1. Aggressiveness assays were also performed on accessions of S. commersonii, a wild species native to Uruguay that is a source of resistance for potato breeding. No significant interactions were found between bacterial strains and potato and S. commersonii genotypes, and differences in aggressiveness among R. solanacearum strains were consistent with previously identified groups based on tomato and potato inoculations. Moreover, variation in responses to R. solanacearum was observed among the S. commersonii accessions tested.


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