scholarly journals Effect of hyperbaric oxygen therapy on esophagojejunal anastomosis healing in rats

2008 ◽  
Vol 23 (6) ◽  
pp. 491-496 ◽  
Author(s):  
João Domingos Lionço ◽  
Lívia Caprara Lionço ◽  
Lucas Torely Filippi ◽  
Clarissa Caprara Lionço ◽  
Bernardo Volkweiss ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Breaking Strength ◽  
Suture Line ◽  
Collagen Concentration ◽  
Esophagojejunal Anastomosis ◽  
Group A ◽  
Anastomotic Fistula ◽  
Group B

PURPOSE: To evaluate hyperbaric oxygen therapy (HBO) after esophagojejunal anastomosis in a rat experimental model and the effect of HBO on the healing of esophagojejunal anastomosis in gastrectomized rats. METHODS: Forty adult male Wistar rats, weighing 322 g to 506 g, were divided into two random groups. In group A (control group), 20 rats were subjected to total gastrectomy. In group B, 20 rats were similarly gastrectomized, but also received postoperative HBO treatment for 90 minutes/day for seven days. All rats were sacrificed on the eighth postoperative day and evaluated according to the following study variables: a) presence of anastomotic fistula; b) evaluation of esophagojejunal anastomosis healing by measuring the breaking strength at the suture line as per Hendriks & Mastboom; and c) determining the collagen concentration in the anastomosis, as per Kovács criteria. For the comparative statistical analysis between groups, Student's "t" test was used. A value of p<0.05 was considered significant. RESULTS: There was a 20% mortality rate. There were five deaths in group A and three in group B (p=N.S.). There was only one anastomotic fistula in each group, and neither caused any morbidity or death. Breaking strength measured at the suture line (p=0.528) and collagen concentration determined at the esophagojejunal anastomosis (p=0.89) were not significantly different between Groups A and B. CONCLUSION: Hyperbaric oxygen therapy did not affect the healing process of esophagojejunal anastomosis.

scholarly journals Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole

2011 ◽  
Vol 26 (3) ◽  
pp. 156-164 ◽  
Author(s):  
Marcela Souza Lima Paulo ◽  
Isabel Cristina Andreatta Lemos Paulo ◽  
Tarcizo Afonso Nunes ◽  
Alcino Lázaro da Silva ◽  
Luiz Cálice Cintra ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Wistar Rats ◽  
Oxygen Therapy ◽  
Inferior Pole ◽  
Group A ◽  
Group B ◽  
Oxygen Group ◽  
Subtotal Splenectomy

PURPOSE: To evaluate the effect of hyperbaric oxygen therapy on the survival and weight of rats submitted to subtotal splenectomy and on the viability and growth of the inferior pole. METHODS: Forty Wistar rats underwent subtotal splenectomy with preservation of the inferior pole and distributed into two groups: Group A (n=20) - not treated with hyperbaric oxygen, Group B (n=20) - treated with hyperbaric oxygen. These groups were divided into two subgroups of 10 animals each, according to the time of euthanasia, 15th and 45th days. The survival and weight of the animals were recorded. The inferior pole was measured, weighed and morphologically analyzed. RESULTS: All animals survived. The weight of the animals increased in all subgroups, but decreased on the 10th day in the subgroups treated with hyperbaric oxygen (p<0.001). The viability of the inferior pole was more evident in animals treated on the 15th day, but did not differ on the 45th day. The growth of the inferior pole has not occurred on the 15th day but on the 45th day after surgery in untreated animals (p<0.01) and treated animals (p<0.05). Vascular and cellular increase in treated animals was significantly higher than in untreated ones. CONCLUSION: Hyperbaric oxygen therapy did not affect the survival of animals but reduced their weight. It improved the viability of the inferior splenic pole, but did not interfere with their growth.

scholarly journals The role of hyperbaric oxygen therapy (HOT) as an otoprotection agent against cisplatin ototoxicity

2008 ◽  
Vol 23 (suppl 1) ◽  
pp. 72-76 ◽  
Author(s):  
Camila C. Yassuda ◽  
Ana Elisa M. Righetti ◽  
Maria Cristina L. Cury ◽  
Miguel Ângelo Hyppolito ◽  
José Antonio A. de Oliveira ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Hair Cells ◽  
Hyperbaric Oxygen Therapy ◽  
Guinea Pigs ◽  
Oxygen Therapy ◽  
Outer Hair Cells ◽  
Sem Images ◽  
Irreversible Damage ◽  
Group A ◽  
Group B

PURPOSE: Hyperbaric oxygen therapy (HOT) consists of intermittent inhalations of 100% oxygen at a pressure higher than 1 atm. It is an important adjuvant therapy in pathological processes like soft tissue infections, radiation injury, gas gangrene, osteomyelitis and decompressive diseases. Cisplatin, a potent antineoplastic drug, widely used in cancer therapy is highly ototoxic causing bilateral, irreversible damage to the hearing of high frequency sounds (4-8 KHz). OBJECTIVE:This experimental study conducted at the Faculty of Medicine of Ribeirao Preto, University of Sao Paulo aims to evaluate Hyperbaric Oxygen Therapy as an otoprotection agent against drug toxicity. METHODS: Albino guinea pigs were divided into two groups: in Group A, 5 animals (10 cochlea) received cisplatin, i. p., 8.0 mg/kg/day during three days and afterwards were submitted to HOT; in Group B, 3 animals (6cochlea) received cisplatin, i. p. 8.0mg/kg/day during three days. Guinea pigs were evaluated by acoustic otoemissions (AOE) and scanning electron microscopy (SEM). RESULTS: Group B animals showed loss of auditory functions as measured by AOE and distorted outer hair cells by SEM. In Group A, outer hair cells shown by SEM images were mostly preserved. CONCLUSION: It is presumed that Hyperbaric Oxygen Therapy has a protector effect against cisplatin ototoxicity.

scholarly journals Research on the Possibility of Treating Streptococcal Infections with Hyperbaric Oxygenation

2020 ◽  
Vol 70 (1) ◽  
pp. 43-46
Author(s):  
Władysław Wolański
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Hyperbaric Oxygenation ◽  
Lethal Effect ◽  
Streptococcal Infections ◽  
Group A

AbstractThe aim of the study was to determine the effect of the application of hyperbaric oxygen therapy on the course of an infection with group A type T-3 hemolytic β streptococcus. Experiments were carried out on Porton white mice and in vitro blood plates. General and local infections with streptococci were induced in animals. The infected animals were treated with hyperbaric oxygenation. The lethal effect of infection was significantly inhibited using hyperbaric oxygenation on the first and second day following the infection.

scholarly journals Hyperbaric Oxygen Therapy in Ischaemic Foot Ulcers in Type 2 Diabetes: A Clinical Trial

2018 ◽  
Vol 12 (1) ◽  
pp. 80-85 ◽  
Author(s):  
Sarah Perren ◽  
Alfred Gatt ◽  
Nikolaos Papanas ◽  
Cynthia Formosa
Keyword(s):  
Type 2 Diabetes ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Foot Ulcer ◽  
Treatment Modalities ◽  
Foot Ulcers ◽  
Ulcer Area ◽  
Group A

Background and Aims: Several treatment modalities and protocols for ischaemic foot ulcers are available. However, little consensus exists on optimal treatment. The aim of this study was to compare Standard Wound Care (SWC) alone vs. SWC with adjunct hyperbaric oxygen therapy (HBOT) in the treatment of ischaemic Diabetic Foot Ulcers (DFUs). Patients and Methods: Twenty-six patients with Type 2 Diabetes Mellitus (T2DM) presenting with a newly diagnosed ischaemic foot ulcer were included. These were divided into group A (SWC with adjunct HBOT) and group B (SWC only). Participants were followed every week for 4 weeks and their ulcers were measured for their surface area and depth to assess any change in wound size. Results: Both treatment arms succeeded in reducing ulcer area and depth (p<0.001). However, ulcer area (p<0.001) and depth (p<0.001) exhibited superior improvement in group A. Conclusion : Adjunctive HBOT appears to improve wound healing in ischaemic DFUs and merits further study.

scholarly journals Downregulates of ICAM1 expression in Myometrium from pregnant Rattus novergicus infected with Tachyzoite of Toxoplasma gondii with Hyperbaric Oxygen Therapy

2019 ◽  
Vol 17 (1) ◽  
pp. 77
Author(s):  
ARIF RAHMAN NURDIANTO ◽  
ARYATI ARYATI ◽  
MOHAMMAD GURITNO SURYOKUSUMO ◽  
MUSASIRIN MUFASIRIN
Keyword(s):  
Toxoplasma Gondii ◽  
Hyperbaric Oxygen ◽  
Rattus Norvegicus ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Sprague Dawley ◽  
Pregnant Rats ◽  
Days Of Therapy ◽  
Post Test ◽  
Group B

<p align="center"><strong>Abstract</strong></p><p><strong>Background:</strong> To find the effect of Hyperbaric Oxygen Therapy in the myometrium expressions of ICAM1 from pregnant Rattus norvegicus infected with Tachyzoite <em>Toxoplasma gondii.</em></p><p><strong>Material and Methods :</strong></p><p>This is an experimental study with a post-test only design on 37 pregnant <em>Rattus norvegicus</em> <em>Sprague Dawley</em>, then the rats were divided into 4 groups. The HBOT group A is pregnant rats infected with tachyzoite received therapy 10 sessions of HBOT 2.4 ATA in 3x30 minutes; the group B is Pregnant only and get no HBOT; the group C is Pregnant and infected with <em>tachyzoite T.gondii</em> but not received HBOT, and the group D is normal pregnant rats. Each infected pregnant rats were given a 10<sup>3 </sup>Tachyzoite of <em>Toxoplasma gondii</em> via intraperitoneal. Examinations of ICAM1 expressions were performed on day-5 after HBOT (twice a day). Rats were killed and the myometrial ICAM1 levels were measured with Immunohistochemistry examination. All data were analyzed with the ANOVA test with SPSS 21 Program.<strong></strong></p><p><strong>Results: </strong>The results show that Hyperbaric Oxygen Therapy can downregulate ICAM1 in the administered of HBO 2.4 ATA for 3x30 minutes in 10 sessions over 5 days of therapy.</p><p><strong>Conclusion:</strong> The study concludes that HBOT can downregulate the expressions of ICAM1 in the myometrium, in the provision of HBO 2.4 ATA for 3x30 minutes in 10 sessions.</p>

scholarly journals Efek Proteksi dari Terapi Oksigen Hiperbarik terhadap Ekspresi Bcl-2 Miometrium Rattus norvegicus Bunting yang Terinfeksi oleh Tachyzoite Toxoplasma gondii

2020 ◽  
Vol 9 (1) ◽  
pp. 85
Author(s):  
Arif Rahman Nurdianto ◽  
Aryati Aryati ◽  
Mohammad Guritno Suryokusumo ◽  
Mufasirin Mufasirin
Keyword(s):  
Toxoplasma Gondii ◽  
Hyperbaric Oxygen ◽  
Rattus Norvegicus ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Control Group ◽  
Pregnant Rats ◽  
Pregnant Rat ◽  
Significant Difference ◽  
Group A

Hyperbaric Oxygen Therapy (HBOT) can increase oxygen delivery to tissues and stimulate the formation of H2O2 as a secondary messenger for phosphorylation of nuclear factor kappa beta (NF-kB) which plays an important role in the transcription of the anti apoptotic gene. This study aimed to determine the effects of Hyperbaric Oxygen Therapy (HBOT) in enhancing the expressions of Bcl-2 in the myometrium of pregnant rats infected by Toxoplasma gondii. This study was an experimental study with a randomized control group of post-test only and designed by 37 pregnant Rattus norvegicus Sprague Dawley. Randomly, the rats were divided into four groups. Group A is infected pregnant rats that exposed by 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group B is non-infected pregnant rats and exposed by 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group C is infected pregnant rats without any exposure. Group D is non-infected pregnant rats without any exposure. Each infected pregnant rat was given a 103 tachyzoite of T.gondii by intraperitoneal injection. Bcl-2 expressions were measured through immunohistochemistry. All data were analyzed using ANOVA test through SPSS 21 program application. There was a significant difference in Bcl-2 expression between Group A and Group C because p<α (p<0.017). HBOT can increase the expression of Bcl-2 from infected and not infected rat myometrium, in the provision of HBOT 2.4 ATA for 3x30 minutes, twice a day for 5 days.

scholarly journals Elevation of Bcl2 expression in artery spiralis of pregnant Rattus novergicus infected with Tachyzoite of Toxoplasma gondii with hyperbaric oxygen therapy

2019 ◽  
Vol 3 (2) ◽  
pp. 157
Author(s):  
Arif Rahman Nurdianto ◽  
Aryati Aryati ◽  
Mohammad Guritno Suryokusumo ◽  
Mufasirin Mufasirin
Keyword(s):  
Toxoplasma Gondii ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Sprague Dawley ◽  
Pregnant Rats ◽  
Significant Difference ◽  
Post Test ◽  
Group A ◽  
Bcl2 Expression

ABSTRACTSpiral artery apoptosis plays a role in the process of abortion. Low Bcl2 expression found in abortion and in spiral artery. In pregnancy, Toxoplasmosis infection is found to increase apoptosis in spiral arteries. Hyperbaric Oxygen Therapy (HBOT) improves the expression of Bcl-2. This study aims to determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of Bcl2 in artery spiralis of pregnant rats infected with tachyzoite of Toxoplasma gondii. This is an experiment with a ‘randomized control group of post-test only design’ on 37 Rattus novergicus Sprague Dawley rats. Rats were divided into four groups. The group A is pregnant rats infected with 103 tachyzoites via intraperitoneal injection and received 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group B is pregnant only and received HBOT. Group C is pregnant and infected with tachyzoite but did not received HBOT. And the last, Group D is pregnant rats with no infection and did not received HBOT. Examinations of Bcl2 expressions were performed on day-5 after HBOT (twice a day). The Bcl2 expression was measured with immunohistochemistry. All data were tested with One-way ANOVA from SPSS 21.  There is an increased expression of Bcl2 spiralis artery in the Group A. There was a significant difference between Group A and Group C with a value of p=0.042. HBOT can increase the expression of Bcl2 from the spiral arteries of rats, in the provision of HBOT 2.4 ATA for 3x30 minutes, 10 times in 5 days.Keywords: Toxoplasma gondii, tachyzoite, Hyperbaric Oxygen Therapy, Bcl2, spiralis arteryCorrespondence to: [email protected]  ABSTRAK Apoptosis arteri spiralis berperan dalam proses aborsi. Ekspresi Bcl2 rendah ditemukan pada aborsi dan di arteri spiral. Pada kehamilan, infeksi Toxoplasmosis ditemukan meningkatkan apoptosis pada arteri spiralis. Terapi Oksigen Hiperbarik (HBOT) meningkatkan ekspresi Bcl-2. Ini adalah penelitian eksperimental dengan desain post-test only pada 37 hamil Rattus novergicus Sprague Dawley, maka tikus dibagi menjadi 4 kelompok. Grup A HBOT adalah tikus hamil yang terinfeksi tachyzoite yang menerima terapi 10 sesi HBOT 2.4 ATA dalam 3x30 menit; kelompok B adalah Hamil saja dan tidak mendapat HBOT; kelompok C Hamil dan terinfeksi tachyzoite T.gondii tetapi tidak menerima HBOT; dan kelompok D adalah tikus hamil normal. Setiap tikus hamil yang terinfeksi diberi 103 Tachyzoite Toxoplasma gondii melalui intraperitoneal. Pemeriksaan ekspresi Bcl2 dilakukan pada hari ke-5 setelah HBOT (dua kali sehari). Tikus terbunuh dan kadar Bcl2 Arteri spiralis diukur dengan pemeriksaan Immunohistokimia. Semua data dianalisis dengan uji ANOVA dengan Program SPSS 21. Pada studi ini menunjukkan terapi Oksigen Hiperbarik dapat meningkatkan Ekspresi Bcl2 Arteri Spiralis dengan dosis 2.4 ATA selama 3x30 menit dalam 10 sesi selama 5 hari terapi. Studi ini menyimpulkan bahwa HBOT dapat meningkatkan ekspresi Bcl2 dalam arteri spiralis, dalam pemberian HBO 2.4 ATA selama 3x30 menit dalam 10 sesi.Kata kunci: HBOT, Arteri spiralis, Bcl2, Tachyzoite Toxoplasma gondiiKorespondensi: [email protected] 

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