Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole

PURPOSE: To evaluate the effect of hyperbaric oxygen therapy on the survival and weight of rats submitted to subtotal splenectomy and on the viability and growth of the inferior pole. METHODS: Forty Wistar rats underwent subtotal splenectomy with preservation of the inferior pole and distributed into two groups: Group A (n=20) - not treated with hyperbaric oxygen, Group B (n=20) - treated with hyperbaric oxygen. These groups were divided into two subgroups of 10 animals each, according to the time of euthanasia, 15th and 45th days. The survival and weight of the animals were recorded. The inferior pole was measured, weighed and morphologically analyzed. RESULTS: All animals survived. The weight of the animals increased in all subgroups, but decreased on the 10th day in the subgroups treated with hyperbaric oxygen (p<0.001). The viability of the inferior pole was more evident in animals treated on the 15th day, but did not differ on the 45th day. The growth of the inferior pole has not occurred on the 15th day but on the 45th day after surgery in untreated animals (p<0.01) and treated animals (p<0.05). Vascular and cellular increase in treated animals was significantly higher than in untreated ones. CONCLUSION: Hyperbaric oxygen therapy did not affect the survival of animals but reduced their weight. It improved the viability of the inferior splenic pole, but did not interfere with their growth.

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Effect of hyperbaric oxygen therapy on esophagojejunal anastomosis healing in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502008000600004 ◽

2008 ◽

Vol 23 (6) ◽

pp. 491-496 ◽

Cited By ~ 3

Author(s):

João Domingos Lionço ◽

Lívia Caprara Lionço ◽

Lucas Torely Filippi ◽

Clarissa Caprara Lionço ◽

Bernardo Volkweiss ◽

...

Keyword(s):

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Breaking Strength ◽

Suture Line ◽

Collagen Concentration ◽

Esophagojejunal Anastomosis ◽

Group A ◽

Anastomotic Fistula ◽

Group B

PURPOSE: To evaluate hyperbaric oxygen therapy (HBO) after esophagojejunal anastomosis in a rat experimental model and the effect of HBO on the healing of esophagojejunal anastomosis in gastrectomized rats. METHODS: Forty adult male Wistar rats, weighing 322 g to 506 g, were divided into two random groups. In group A (control group), 20 rats were subjected to total gastrectomy. In group B, 20 rats were similarly gastrectomized, but also received postoperative HBO treatment for 90 minutes/day for seven days. All rats were sacrificed on the eighth postoperative day and evaluated according to the following study variables: a) presence of anastomotic fistula; b) evaluation of esophagojejunal anastomosis healing by measuring the breaking strength at the suture line as per Hendriks & Mastboom; and c) determining the collagen concentration in the anastomosis, as per Kovács criteria. For the comparative statistical analysis between groups, Student's "t" test was used. A value of p<0.05 was considered significant. RESULTS: There was a 20% mortality rate. There were five deaths in group A and three in group B (p=N.S.). There was only one anastomotic fistula in each group, and neither caused any morbidity or death. Breaking strength measured at the suture line (p=0.528) and collagen concentration determined at the esophagojejunal anastomosis (p=0.89) were not significantly different between Groups A and B. CONCLUSION: Hyperbaric oxygen therapy did not affect the healing process of esophagojejunal anastomosis.

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The role of hyperbaric oxygen therapy (HOT) as an otoprotection agent against cisplatin ototoxicity

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502008000700013 ◽

2008 ◽

Vol 23 (suppl 1) ◽

pp. 72-76 ◽

Cited By ~ 6

Author(s):

Camila C. Yassuda ◽

Ana Elisa M. Righetti ◽

Maria Cristina L. Cury ◽

Miguel Ângelo Hyppolito ◽

José Antonio A. de Oliveira ◽

...

Keyword(s):

Hyperbaric Oxygen ◽

Hair Cells ◽

Hyperbaric Oxygen Therapy ◽

Guinea Pigs ◽

Oxygen Therapy ◽

Outer Hair Cells ◽

Sem Images ◽

Irreversible Damage ◽

Group A ◽

Group B

PURPOSE: Hyperbaric oxygen therapy (HOT) consists of intermittent inhalations of 100% oxygen at a pressure higher than 1 atm. It is an important adjuvant therapy in pathological processes like soft tissue infections, radiation injury, gas gangrene, osteomyelitis and decompressive diseases. Cisplatin, a potent antineoplastic drug, widely used in cancer therapy is highly ototoxic causing bilateral, irreversible damage to the hearing of high frequency sounds (4-8 KHz). OBJECTIVE:This experimental study conducted at the Faculty of Medicine of Ribeirao Preto, University of Sao Paulo aims to evaluate Hyperbaric Oxygen Therapy as an otoprotection agent against drug toxicity. METHODS: Albino guinea pigs were divided into two groups: in Group A, 5 animals (10 cochlea) received cisplatin, i. p., 8.0 mg/kg/day during three days and afterwards were submitted to HOT; in Group B, 3 animals (6cochlea) received cisplatin, i. p. 8.0mg/kg/day during three days. Guinea pigs were evaluated by acoustic otoemissions (AOE) and scanning electron microscopy (SEM). RESULTS: Group B animals showed loss of auditory functions as measured by AOE and distorted outer hair cells by SEM. In Group A, outer hair cells shown by SEM images were mostly preserved. CONCLUSION: It is presumed that Hyperbaric Oxygen Therapy has a protector effect against cisplatin ototoxicity.

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Research on the Possibility of Treating Streptococcal Infections with Hyperbaric Oxygenation

Polish Hyperbaric Research ◽

10.2478/phr-2020-0003 ◽

2020 ◽

Vol 70 (1) ◽

pp. 43-46

Author(s):

Władysław Wolański

Keyword(s):

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Hyperbaric Oxygenation ◽

Lethal Effect ◽

Streptococcal Infections ◽

Group A

AbstractThe aim of the study was to determine the effect of the application of hyperbaric oxygen therapy on the course of an infection with group A type T-3 hemolytic β streptococcus. Experiments were carried out on Porton white mice and in vitro blood plates. General and local infections with streptococci were induced in animals. The infected animals were treated with hyperbaric oxygenation. The lethal effect of infection was significantly inhibited using hyperbaric oxygenation on the first and second day following the infection.

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Re: “Hyperbaric Oxygen Therapy as Further Adjunctive Therapy in Group A Streptococcus Periorbital Necrotizing Fasciitis”

Ophthalmic Plastic and Reconstructive Surgery ◽

10.1097/iop.0b013e3181e26882 ◽

2010 ◽

Vol 26 (6) ◽

pp. 505

Author(s):

Vinay K. Aakalu ◽

Kiran Sajja ◽

James L. Cook ◽

Amjad Z. Ahmad

Keyword(s):

Necrotizing Fasciitis ◽

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Adjunctive Therapy ◽

Oxygen Therapy ◽

Group A Streptococcus ◽

Group A

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The Effect of Hyperbaric Oxygen Therapy to the Amount of Lymphocytes in Oral Candidiasis Immunosuppressed Model

DENTA ◽

10.30649/denta.v12i2.164 ◽

2019 ◽

Vol 12 (2) ◽

pp. 36

Author(s):

Agni Febrina Pargaputri ◽

Dwi Andriani

Keyword(s):

Immune System ◽

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Wistar Rats ◽

Oxygen Therapy ◽

Oral Candidiasis ◽

Control Group ◽

Control Group Design ◽

Significant Difference ◽

The Difference

Background: Oral candidiasis generally caused by Candida albicans with prevalence of 30-50%. C. Albicans proliferation in the oral cavity was canducted by decreasing immune system of the host. Lymphocytes activation in immune system happened when there is a contact between host cell with cell wall of C. albicans as a result of C. albicans antigen. One of therapy to suppress the Candida infection sistemically is by giving hyperbaric oxygen therapy. Purpose: To determine the effects of giving hyperbaric oxygen therapy to the amount of lymphocytes in oral candidiasis immunosuppressed models. Methods: This research was post test only control group design. We used 12 wistar rats which were divided into 3 groups(n=4/3): K-(normal/ healthy wistar rats), K+(oral candidiasis immunosuppressed wistar rats), P1(oral candidiasis immunosuppressed wistar rats which were given hyperbaric oxygen therapy 5 days). K+ and P1 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetrasiklin 1 mg/day/rat. On the 3rd day immunosuppression, the rats were inducted with C.albicans smeared in the dorsum linguae once every two days for 12 days. Results: The data were analyzed with one way Anova test and showed significant difference among groups (p<0,05). To show the difference between each groups we used LSD test and showed significant difference (p<0,05) between K+ compared with P1, and K+ compared with K-. Conclusion: Hyperbaric oxygen therapy gives effect to the amount of lymphocytes in oral candidiasis immunosuppressed models. Keywords: Oral candidiasis, immunosuppressed, hyperbaric oxygen therapy, lymphocytes Correspondence: Agni Febrina Pargaputri, Department of Oral Biology Faculty of Dentistry, Jl. Arif Rahman Hakim 150, Surabaya, Indonesia. Phone: 031-5945864, fax: 031-5912191, Email: agni.febrina@hangtuah.ac.id

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Double-blind trials in hyperbaric medicine: A narrative review on past experiences and considerations in designing sham hyperbaric treatment

Clinical Trials ◽

10.1177/1740774518776952 ◽

2018 ◽

Vol 15 (5) ◽

pp. 462-476 ◽

Cited By ~ 6

Author(s):

Nina CA Lansdorp ◽

Rob A van Hulst

Keyword(s):

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Lower Pressure ◽

Controlled Trials ◽

Double Blind ◽

Hyperbaric Treatment ◽

Hyperbaric Medicine ◽

Oxygen Group ◽

Blind Trials

Background Hyperbaric oxygen therapy, which consists of breathing 100% oxygen under a higher atmospheric pressure than normal, is utilized worldwide in the treatment of several diseases. With the growing demand for evidence-based research, hyperbaric oxygen therapy has been criticized for delivering too little high-quality research, mainly in the form of randomized controlled trials. While not always indispensable, the addition of a sham-controlled group to such a trial can contribute to the quality of the research. However, the design of a sham (hyperbaric) treatment is associated with several considerations regarding adequate blinding and the use of pressure and oxygen. This narrative review discusses information on the sham profile and the blinding and safety of double-blind trials in hyperbaric medicine, irrespective of the indication for treatment. Methods MEDLINE, Embase and CENTRAL were searched for sham-controlled trials on hyperbaric oxygen therapy. The control treatment was considered sham if patients were blinded to their allocation and treatment took place in a hyperbaric chamber, with no restrictions regarding pressurization, oxygen levels or indication. Studies involving children or only one session of hyperbaric oxygen were excluded. Information on (the choice of) treatment profile, blinding measures, patient’s perception regarding allocation and safety issues was extracted from eligible studies. Results A total of 42 eligible trials were included. The main strategies for sham treatment were (1) use of a lower pressure than that of the hyperbaric oxygen group, while breathing 21% oxygen; (2) use of the same pressure as the hyperbaric oxygen group, while breathing an adjusted percentage of oxygen; and (3) use of the same pressure as the hyperbaric oxygen group, while breathing 21% oxygen. The advantages and disadvantages of each strategy are discussed using the information provided by the trials. Conclusion Based on this review, using a lower pressure than the hyperbaric oxygen group while breathing 21% oxygen best matches the inertness of the placebo. Although studies show that use of a lower pressure does allow adequate blinding, this is associated with more practical issues than with the other strategies. The choice of which sham profile to use requires careful consideration; moreover, to ensure proper performance, a clear and detailed protocol is also required.

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Hyperbaric Oxygen Therapy as Further Adjunctive Therapy in the Treatment of Periorbital Necrotizing Fasciitis Caused by Group A Streptococcus

Ophthalmic Plastic and Reconstructive Surgery ◽

10.1097/iop.0b013e3181e26821 ◽

2010 ◽

Vol 26 (6) ◽

pp. 504-505 ◽

Cited By ~ 2

Author(s):

Davide Lazzeri ◽

Stefano Lazzeri ◽

Michele Figus ◽

Tommaso Agostini ◽

Marco Nardi ◽

...

Keyword(s):

Necrotizing Fasciitis ◽

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Adjunctive Therapy ◽

Oxygen Therapy ◽

Group A Streptococcus ◽

Group A

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Hyperbaric Oxygen Therapy in Ischaemic Foot Ulcers in Type 2 Diabetes: A Clinical Trial

The Open Cardiovascular Medicine Journal ◽

10.2174/1874192401812010080 ◽

2018 ◽

Vol 12 (1) ◽

pp. 80-85 ◽

Cited By ~ 6

Author(s):

Sarah Perren ◽

Alfred Gatt ◽

Nikolaos Papanas ◽

Cynthia Formosa

Keyword(s):

Type 2 Diabetes ◽

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Foot Ulcer ◽

Treatment Modalities ◽

Foot Ulcers ◽

Ulcer Area ◽

Group A

Background and Aims: Several treatment modalities and protocols for ischaemic foot ulcers are available. However, little consensus exists on optimal treatment. The aim of this study was to compare Standard Wound Care (SWC) alone vs. SWC with adjunct hyperbaric oxygen therapy (HBOT) in the treatment of ischaemic Diabetic Foot Ulcers (DFUs). Patients and Methods: Twenty-six patients with Type 2 Diabetes Mellitus (T2DM) presenting with a newly diagnosed ischaemic foot ulcer were included. These were divided into group A (SWC with adjunct HBOT) and group B (SWC only). Participants were followed every week for 4 weeks and their ulcers were measured for their surface area and depth to assess any change in wound size. Results: Both treatment arms succeeded in reducing ulcer area and depth (p<0.001). However, ulcer area (p<0.001) and depth (p<0.001) exhibited superior improvement in group A. Conclusion : Adjunctive HBOT appears to improve wound healing in ischaemic DFUs and merits further study.

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Preservative spleen surgery and hyperbaric oxygen therapy

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502007000700006 ◽

2007 ◽

Vol 22 (suppl 1) ◽

pp. 21-28 ◽

Cited By ~ 8

Author(s):

Isabel Cristina Andreatta Lemos Paulo ◽

Danilo Nagib Salomão Paulo ◽

Luiz Cálice Cintra ◽

Maria Carmem Silva Santos ◽

Hildegardo Rodrigues ◽

...

Keyword(s):

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Histological Study ◽

Microscopic Appearance ◽

Inferior Pole ◽

Study Results ◽

Male Wistar Rats ◽

Group 3 ◽

Group 2

PURPOSE: To assess functional and morphological aspects of spleen auto-implants and of the splenic inferior pole of rats, post-operatively treated or not with hyperbaric oxygen, as well as the survival of these animals, were studied. METHODS: Seventy-eight male Wistar rats, weighing between 192 and 283 g ( 238,3 ± 9,6g), were randomly distributed into three groups: Group1-(n=20), spleen manipulation; group 2-(n=36), spleen auto-implantation; group3-(n= 22), subtotal splenectomy preserving the inferior pole. Each group was subdivided as follows: subgroup a, not submitted to hyperbaric oxygen therapy: 1a(n=10), 2a(n=21), 3a(n= 13); subgroup b, submitted to the therapy: 1b(n=10), 2b(n=15), 3b(n=9). Blood was collected pre-operatively and 11 days after surgery, for the estimation of lipids and immunoglobulins and the counting of platelets and Howell-Jolly corpuscles. The spleen and remains were taken for histological study. RESULTS: The number of surviving animals was significantly higher in groups 1(p<0,01) and 3(p<0,05) relative to those of subgroup 2a. Total cholesterol and the LDL fraction increased significantly in subgroup 2a (p<0,01) and 3a (p<0,05), and remained unaltered in subgroups 2b e 3b. IgM decreased more significantly in subgroup 2 than in subgroup 3 (p<0,001 vs p<0,01). The increase of platelet numbers and the appearance of Howell Jolly corpuscles was smaller in subgroup 2b compared to subgroup 2a , and in group 3 compared to group aqui-> 2. The macro and microscopic appearance in subgroup 2b were more viable than in subgroup 2a, and that of group 3 more viable than in group 2. The survival of the animals carrying their whole spleen or its inferior pole was more frequent than that of the auto-implanted animals. CONCLUSION: Functionality and viability of the whole spleen or of its inferior pole, were better than in the auto-implanted animals. Hyperbaric oxygentherapy contributed to increased survival frequency of auto -implanted animals, and to improve the functionality and viability of the auto-implants and the function of the inferior splenic pole, and did not interfere in animals carrying their whole spleen.

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