scholarly journals Downregulates of ICAM1 expression in Myometrium from pregnant Rattus novergicus infected with Tachyzoite of Toxoplasma gondii with Hyperbaric Oxygen Therapy

2019 ◽  
Vol 17 (1) ◽  
pp. 77
Author(s):  
ARIF RAHMAN NURDIANTO ◽  
ARYATI ARYATI ◽  
MOHAMMAD GURITNO SURYOKUSUMO ◽  
MUSASIRIN MUFASIRIN
Keyword(s):  
Toxoplasma Gondii ◽  
Hyperbaric Oxygen ◽  
Rattus Norvegicus ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Sprague Dawley ◽  
Pregnant Rats ◽  
Days Of Therapy ◽  
Post Test ◽  
Group B

<p align="center"><strong>Abstract</strong></p><p><strong>Background:</strong> To find the effect of Hyperbaric Oxygen Therapy in the myometrium expressions of ICAM1 from pregnant Rattus norvegicus infected with Tachyzoite <em>Toxoplasma gondii.</em></p><p><strong>Material and Methods :</strong></p><p>This is an experimental study with a post-test only design on 37 pregnant <em>Rattus norvegicus</em> <em>Sprague Dawley</em>, then the rats were divided into 4 groups. The HBOT group A is pregnant rats infected with tachyzoite received therapy 10 sessions of HBOT 2.4 ATA in 3x30 minutes; the group B is Pregnant only and get no HBOT; the group C is Pregnant and infected with <em>tachyzoite T.gondii</em> but not received HBOT, and the group D is normal pregnant rats. Each infected pregnant rats were given a 10<sup>3 </sup>Tachyzoite of <em>Toxoplasma gondii</em> via intraperitoneal. Examinations of ICAM1 expressions were performed on day-5 after HBOT (twice a day). Rats were killed and the myometrial ICAM1 levels were measured with Immunohistochemistry examination. All data were analyzed with the ANOVA test with SPSS 21 Program.<strong></strong></p><p><strong>Results: </strong>The results show that Hyperbaric Oxygen Therapy can downregulate ICAM1 in the administered of HBO 2.4 ATA for 3x30 minutes in 10 sessions over 5 days of therapy.</p><p><strong>Conclusion:</strong> The study concludes that HBOT can downregulate the expressions of ICAM1 in the myometrium, in the provision of HBO 2.4 ATA for 3x30 minutes in 10 sessions.</p>

scholarly journals Elevation of Bcl2 expression in artery spiralis of pregnant Rattus novergicus infected with Tachyzoite of Toxoplasma gondii with hyperbaric oxygen therapy

2019 ◽  
Vol 3 (2) ◽  
pp. 157
Author(s):  
Arif Rahman Nurdianto ◽  
Aryati Aryati ◽  
Mohammad Guritno Suryokusumo ◽  
Mufasirin Mufasirin
Keyword(s):  
Toxoplasma Gondii ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Sprague Dawley ◽  
Pregnant Rats ◽  
Significant Difference ◽  
Post Test ◽  
Group A ◽  
Bcl2 Expression

ABSTRACTSpiral artery apoptosis plays a role in the process of abortion. Low Bcl2 expression found in abortion and in spiral artery. In pregnancy, Toxoplasmosis infection is found to increase apoptosis in spiral arteries. Hyperbaric Oxygen Therapy (HBOT) improves the expression of Bcl-2. This study aims to determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of Bcl2 in artery spiralis of pregnant rats infected with tachyzoite of Toxoplasma gondii. This is an experiment with a ‘randomized control group of post-test only design’ on 37 Rattus novergicus Sprague Dawley rats. Rats were divided into four groups. The group A is pregnant rats infected with 103 tachyzoites via intraperitoneal injection and received 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group B is pregnant only and received HBOT. Group C is pregnant and infected with tachyzoite but did not received HBOT. And the last, Group D is pregnant rats with no infection and did not received HBOT. Examinations of Bcl2 expressions were performed on day-5 after HBOT (twice a day). The Bcl2 expression was measured with immunohistochemistry. All data were tested with One-way ANOVA from SPSS 21.  There is an increased expression of Bcl2 spiralis artery in the Group A. There was a significant difference between Group A and Group C with a value of p=0.042. HBOT can increase the expression of Bcl2 from the spiral arteries of rats, in the provision of HBOT 2.4 ATA for 3x30 minutes, 10 times in 5 days.Keywords: Toxoplasma gondii, tachyzoite, Hyperbaric Oxygen Therapy, Bcl2, spiralis arteryCorrespondence to: [email protected]  ABSTRAK Apoptosis arteri spiralis berperan dalam proses aborsi. Ekspresi Bcl2 rendah ditemukan pada aborsi dan di arteri spiral. Pada kehamilan, infeksi Toxoplasmosis ditemukan meningkatkan apoptosis pada arteri spiralis. Terapi Oksigen Hiperbarik (HBOT) meningkatkan ekspresi Bcl-2. Ini adalah penelitian eksperimental dengan desain post-test only pada 37 hamil Rattus novergicus Sprague Dawley, maka tikus dibagi menjadi 4 kelompok. Grup A HBOT adalah tikus hamil yang terinfeksi tachyzoite yang menerima terapi 10 sesi HBOT 2.4 ATA dalam 3x30 menit; kelompok B adalah Hamil saja dan tidak mendapat HBOT; kelompok C Hamil dan terinfeksi tachyzoite T.gondii tetapi tidak menerima HBOT; dan kelompok D adalah tikus hamil normal. Setiap tikus hamil yang terinfeksi diberi 103 Tachyzoite Toxoplasma gondii melalui intraperitoneal. Pemeriksaan ekspresi Bcl2 dilakukan pada hari ke-5 setelah HBOT (dua kali sehari). Tikus terbunuh dan kadar Bcl2 Arteri spiralis diukur dengan pemeriksaan Immunohistokimia. Semua data dianalisis dengan uji ANOVA dengan Program SPSS 21. Pada studi ini menunjukkan terapi Oksigen Hiperbarik dapat meningkatkan Ekspresi Bcl2 Arteri Spiralis dengan dosis 2.4 ATA selama 3x30 menit dalam 10 sesi selama 5 hari terapi. Studi ini menyimpulkan bahwa HBOT dapat meningkatkan ekspresi Bcl2 dalam arteri spiralis, dalam pemberian HBO 2.4 ATA selama 3x30 menit dalam 10 sesi.Kata kunci: HBOT, Arteri spiralis, Bcl2, Tachyzoite Toxoplasma gondiiKorespondensi: [email protected] 

scholarly journals Efek Proteksi dari Terapi Oksigen Hiperbarik terhadap Ekspresi Bcl-2 Miometrium Rattus norvegicus Bunting yang Terinfeksi oleh Tachyzoite Toxoplasma gondii

2020 ◽  
Vol 9 (1) ◽  
pp. 85
Author(s):  
Arif Rahman Nurdianto ◽  
Aryati Aryati ◽  
Mohammad Guritno Suryokusumo ◽  
Mufasirin Mufasirin
Keyword(s):  
Toxoplasma Gondii ◽  
Hyperbaric Oxygen ◽  
Rattus Norvegicus ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Control Group ◽  
Pregnant Rats ◽  
Pregnant Rat ◽  
Significant Difference ◽  
Group A

Hyperbaric Oxygen Therapy (HBOT) can increase oxygen delivery to tissues and stimulate the formation of H2O2 as a secondary messenger for phosphorylation of nuclear factor kappa beta (NF-kB) which plays an important role in the transcription of the anti apoptotic gene. This study aimed to determine the effects of Hyperbaric Oxygen Therapy (HBOT) in enhancing the expressions of Bcl-2 in the myometrium of pregnant rats infected by Toxoplasma gondii. This study was an experimental study with a randomized control group of post-test only and designed by 37 pregnant Rattus norvegicus Sprague Dawley. Randomly, the rats were divided into four groups. Group A is infected pregnant rats that exposed by 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group B is non-infected pregnant rats and exposed by 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group C is infected pregnant rats without any exposure. Group D is non-infected pregnant rats without any exposure. Each infected pregnant rat was given a 103 tachyzoite of T.gondii by intraperitoneal injection. Bcl-2 expressions were measured through immunohistochemistry. All data were analyzed using ANOVA test through SPSS 21 program application. There was a significant difference in Bcl-2 expression between Group A and Group C because p<α (p<0.017). HBOT can increase the expression of Bcl-2 from infected and not infected rat myometrium, in the provision of HBOT 2.4 ATA for 3x30 minutes, twice a day for 5 days.

scholarly journals Effect of hyperbaric oxygen therapy on esophagojejunal anastomosis healing in rats

2008 ◽  
Vol 23 (6) ◽  
pp. 491-496 ◽  
Author(s):  
João Domingos Lionço ◽  
Lívia Caprara Lionço ◽  
Lucas Torely Filippi ◽  
Clarissa Caprara Lionço ◽  
Bernardo Volkweiss ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Breaking Strength ◽  
Suture Line ◽  
Collagen Concentration ◽  
Esophagojejunal Anastomosis ◽  
Group A ◽  
Anastomotic Fistula ◽  
Group B

PURPOSE: To evaluate hyperbaric oxygen therapy (HBO) after esophagojejunal anastomosis in a rat experimental model and the effect of HBO on the healing of esophagojejunal anastomosis in gastrectomized rats. METHODS: Forty adult male Wistar rats, weighing 322 g to 506 g, were divided into two random groups. In group A (control group), 20 rats were subjected to total gastrectomy. In group B, 20 rats were similarly gastrectomized, but also received postoperative HBO treatment for 90 minutes/day for seven days. All rats were sacrificed on the eighth postoperative day and evaluated according to the following study variables: a) presence of anastomotic fistula; b) evaluation of esophagojejunal anastomosis healing by measuring the breaking strength at the suture line as per Hendriks & Mastboom; and c) determining the collagen concentration in the anastomosis, as per Kovács criteria. For the comparative statistical analysis between groups, Student's "t" test was used. A value of p<0.05 was considered significant. RESULTS: There was a 20% mortality rate. There were five deaths in group A and three in group B (p=N.S.). There was only one anastomotic fistula in each group, and neither caused any morbidity or death. Breaking strength measured at the suture line (p=0.528) and collagen concentration determined at the esophagojejunal anastomosis (p=0.89) were not significantly different between Groups A and B. CONCLUSION: Hyperbaric oxygen therapy did not affect the healing process of esophagojejunal anastomosis.

scholarly journals Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole

2011 ◽  
Vol 26 (3) ◽  
pp. 156-164 ◽  
Author(s):  
Marcela Souza Lima Paulo ◽  
Isabel Cristina Andreatta Lemos Paulo ◽  
Tarcizo Afonso Nunes ◽  
Alcino Lázaro da Silva ◽  
Luiz Cálice Cintra ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Wistar Rats ◽  
Oxygen Therapy ◽  
Inferior Pole ◽  
Group A ◽  
Group B ◽  
Oxygen Group ◽  
Subtotal Splenectomy

PURPOSE: To evaluate the effect of hyperbaric oxygen therapy on the survival and weight of rats submitted to subtotal splenectomy and on the viability and growth of the inferior pole. METHODS: Forty Wistar rats underwent subtotal splenectomy with preservation of the inferior pole and distributed into two groups: Group A (n=20) - not treated with hyperbaric oxygen, Group B (n=20) - treated with hyperbaric oxygen. These groups were divided into two subgroups of 10 animals each, according to the time of euthanasia, 15th and 45th days. The survival and weight of the animals were recorded. The inferior pole was measured, weighed and morphologically analyzed. RESULTS: All animals survived. The weight of the animals increased in all subgroups, but decreased on the 10th day in the subgroups treated with hyperbaric oxygen (p<0.001). The viability of the inferior pole was more evident in animals treated on the 15th day, but did not differ on the 45th day. The growth of the inferior pole has not occurred on the 15th day but on the 45th day after surgery in untreated animals (p<0.01) and treated animals (p<0.05). Vascular and cellular increase in treated animals was significantly higher than in untreated ones. CONCLUSION: Hyperbaric oxygen therapy did not affect the survival of animals but reduced their weight. It improved the viability of the inferior splenic pole, but did not interfere with their growth.

scholarly journals Pengaruh Prosedur Orientasi terhadap Tingkat Kecemasan pada Pasien yang Menjalani Terapi Oksigen Hiperbarik (TOHB) di Rumah Sakit Paru Jember

2018 ◽  
Vol 6 (2) ◽  
pp. 292
Author(s):  
Pravita Dwi Ariyani ◽  
Dodi Wijaya ◽  
Ahmad Rifai
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Sampling Technique ◽  
Treatment Method ◽  
Wilcoxon Test ◽  
Pure Oxygen ◽  
Post Test ◽  
Normal Atmospheric Pressure ◽  
Hyperbaric Therapy

Hyperbaric oxygen therapy is a treatment method in patients with inhaling pure oxygen at pressuresgreater than normal atmospheric pressure. Patients who first followed the hyperbaric therapy willgenerally feel anxiety due to side effects of hyperbaric therapy. Orientation procedure can be used todecrease level of anxiety of patients. The purpose of this study was to analyze the effect of theorientation procedure on the level of anxiety of patients undergoing hyperbaric oxygen therapy atRumah Sakit Paru Jember. The design of study was pre experimental design with one group pretestand post test. The sampling technique was consecutive sampling involving 32 respondents. Datawere analyzed with Wilcoxon Test. The result showed a significant decrease of moderate anxiety tomild anxiety with implementation of orientation procedure (p=0.000). It can be concluded that thereis an effect of the orientation procedure on the level of anxiety of patients undergoing hyperbaricoxygen therapy at Rumah Sakit Paru Jember. The provision of adequate information and theintroduction of a hyperbaric chamber every corner of the room so that the patient is able to adapt tothe first experience, so that the necessary role of nurses in assisting the patient to provide referralsfor patients who want to undergoing hyperbaric therapy in order to follow the therapy process untilcomplete. Orientation procedure of hyperbaric therapy can be applied as one of method to decreaselevel of anxiety of patients undergoing hyperbaric oxygen therapyKeywords: orientation procedure, hyperbaric oxygen therapy, level of anxiety

scholarly journals EFEKTIVITAS TERAPI OKSIGEN HIPERBARIK TERHADAP KADAR GULA DARAH PASIEN DIABETES MELLITUS

2017 ◽  
Vol 3 (1) ◽  
pp. 19
Author(s):  
Inggried Angelica Valentina Wiliyams Peni
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Blood Sugar Level ◽  
Oxygen Therapy ◽  
Cross Sectional ◽  
Post Test ◽  
Sugar Levels

ABSTRAK Indonesia termasuk dalam 7 negara dengan prevalensi diabetes tertinggi di dunia menurut data dari International Diabetes Federasi (IDF) tahun 2013. Tujuan dari penelitian ini adalah untuk mengetahui apakah ada perubahan kadar gula pasien DM tipe 2 sebelum dan setelah terapi oksigen hiperbarik. Metode penelitian ini adalah metode kuantitatif yang bersifat cross sectional dengan desain pre expereimental one group pre-test and post- test design. Populasi penelitian ini adalah pasien DM tipe 2 di Rumah Sakit Advent Bandung, sebanyak 8 orang yang berjenis kelamin perempuan dan yang berusia ≥40 tahun menjadi sampel yang telah dipilih secara purposive sampling. Hasil penelitian, rata-rata kadar gula darah pasien sebelum terapi adalah 257,25 mg/dL dan rata-rata kadar gula darah pasien sesudah terapi adalah 176,00 mg/dL. Hasil uji statistik untuk keefektivan terapi oksigen terhadap kadar gula darah telah diukur menggunakan paired T-test memiliki nilai probabilitas 0,000 < 0,05. Kesimpulan penelitian ini adalah terapi oksigen hiperbarik mempunyai pengaruh terhadap menurunkan kadar gula darah sewaktu yang signifikan. Untuk menggembangkan penelitian ini diharapkan kepada peneliti selanjutnya untuk memperhatikan pada jumlah kalori yang dikonsumsi dan interval lama pasien terdiagnosa DM atau melakukan penelitian ulang.   Kata Kunci : Terapi Oksigen Hiperbarik, Diabetes Mellitus, Kadar Gula Darah   ABSTRACT Indonesia is among the 7 countries with the highest prevalence of diabetes in the world according to data from the International Diabetes Federation (IDF) in 2013. The purpose of this study was to determine whether there is a change in blood sugar levels of patient with type 2 diabetes before and after hyperbaric oxygen therapy. This research method is quantitative method which is cross sectional with pre experimental design one group pre-test and post-test. The population of this study is the patient with type 2 diabetes mellitus in Adventist Bandung Hospital, 8 people of woman and over the age of 40 years into a sample that has been selected by purposive sampling. The result of this study, the mean of patient’s blood sugar level before therapy was 257,25 mg/dl and the mean of patient’s blood sugar level after therapy was 176,00 mg/dl. Statistical test result for the effectiveness of oxygen therapy to blood sugar levels were measured using paired t-test having a probability value 0,000 < 0,05. The conclusion of this study is hyperbaric oxygen therapy has an effect on decreasing blood sugar level. To develop this study is expected for next researchers to pay attention to the number of calories consumed and the intervals of patient diagnosed diabetes mellitus or re-do this study. Keywords: Hyperbaric Oxygen Therapy, Blood Sugar Levels, Diabetes Mellitus

scholarly journals The role of hyperbaric oxygen therapy (HOT) as an otoprotection agent against cisplatin ototoxicity

2008 ◽  
Vol 23 (suppl 1) ◽  
pp. 72-76 ◽  
Author(s):  
Camila C. Yassuda ◽  
Ana Elisa M. Righetti ◽  
Maria Cristina L. Cury ◽  
Miguel Ângelo Hyppolito ◽  
José Antonio A. de Oliveira ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Hair Cells ◽  
Hyperbaric Oxygen Therapy ◽  
Guinea Pigs ◽  
Oxygen Therapy ◽  
Outer Hair Cells ◽  
Sem Images ◽  
Irreversible Damage ◽  
Group A ◽  
Group B

PURPOSE: Hyperbaric oxygen therapy (HOT) consists of intermittent inhalations of 100% oxygen at a pressure higher than 1 atm. It is an important adjuvant therapy in pathological processes like soft tissue infections, radiation injury, gas gangrene, osteomyelitis and decompressive diseases. Cisplatin, a potent antineoplastic drug, widely used in cancer therapy is highly ototoxic causing bilateral, irreversible damage to the hearing of high frequency sounds (4-8 KHz). OBJECTIVE:This experimental study conducted at the Faculty of Medicine of Ribeirao Preto, University of Sao Paulo aims to evaluate Hyperbaric Oxygen Therapy as an otoprotection agent against drug toxicity. METHODS: Albino guinea pigs were divided into two groups: in Group A, 5 animals (10 cochlea) received cisplatin, i. p., 8.0 mg/kg/day during three days and afterwards were submitted to HOT; in Group B, 3 animals (6cochlea) received cisplatin, i. p. 8.0mg/kg/day during three days. Guinea pigs were evaluated by acoustic otoemissions (AOE) and scanning electron microscopy (SEM). RESULTS: Group B animals showed loss of auditory functions as measured by AOE and distorted outer hair cells by SEM. In Group A, outer hair cells shown by SEM images were mostly preserved. CONCLUSION: It is presumed that Hyperbaric Oxygen Therapy has a protector effect against cisplatin ototoxicity.

scholarly journals PENGARUH KOMBINASI TERAPI OKSIGEN HIPERBARIK DAN PROPOLIS TERHADAP DIAMETER PEMBULUH DARAH DI DAERAH TARIKAN LIGAMEN PERIODONTAL SELAMA PERGERAKAN GIGI UNTUK MENCEGAH RELAPS

DENTA ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Pambudi Rahardjo ◽  
Maria Lisdiana Tandjung ◽  
Dandy Bayu Angkasa
Keyword(s):  
Blood Vessels ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Periodontal Ligament ◽  
Oxygen Therapy ◽  
Control Group ◽  
Control Group Design ◽  
Propolis Extract ◽  
Post Test ◽  
Pressure Area

<strong><em>Background</em></strong><em>: The mechanical force of orthodontic device causes occurrence of a tension and a pressure area in the periodontal ligament. In the tension area, an inflammatory response characterized by vasodilation of blood vessels will occur. The function of blood vessel is to flow blood and other nutrients to accelerate the periodontal ligament remodeling process, so that relapse can be prevented. <strong>Objective:</strong> To determine the effect of combination of propolis 3%, 5% for 14 days and HBOT 2,4 ATA 3x30 minutes with 5 minutes interval for 7 days in the tension area of the periodontal ligament as an effort to prevent relapse. <strong>Materials and Methods</strong>: A randomized post-test only control group design was used in this study. 42 male guinea pigs (cavia cobaya) are divided into 7 groups K- (without treatment), K + (using separator), P1 (using separator and propolis extract 3%), P2 (using separator and propolis extract 5%), P3 (using separator and hyperbaric oxygen therapy), P4 (using separator and combination of hyperbaric oxygen therapy and 3% propolis extract), and P5 (using separator and combination of hyperbaric oxygen therapy and 5% propolis extract) . <strong>Results</strong>: Data shows that mean of diameter of blood vessels increased from the smallest K- (41,8417±0,84511), K+(61,77±1,254482), P1(72,5983±0,68869), P2(73,2333±1,24481), P3(74,2583± 0,62030), P4(91,47±0,76585), to the largest in P5(91,5483±0,96178). One-way Anova and LSD test showed significant differences in blood vessels diameter (p &lt;0.05). <strong>Conclusion</strong>: The combination of propolis 3% and 5% and HBOT have significant effect in increasing blood vessels diameter in the tension area of the periodontal ligament</em>

Sign in / Sign up

Export Citation Format

Share Document