scholarly journals Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

2012 ◽  
Vol 27 (4) ◽  
pp. 325-332 ◽  
Author(s):  
Daniel Nunes e Silva ◽  
Andréia Conceição Milan Brochado Antoniolli da Silva ◽  
Ricardo Dutra Aydos ◽  
Fausto Viterbo ◽  
Elenir Rose Jardim Cury Pontes ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Fibrin Glue ◽  
Tibial Nerve ◽  
Nerve Repair ◽  
Nerve Section ◽  
Nerve Growth ◽  
Group A ◽  
Group B ◽  
Group D

PURPOSE: To determine the effects of end-to-side nerve repair performed only with fibrin glue containing nerve growth in rats. METHODS: Seventy two Wistar rats were divided into six equal groups: group A was not submitted to nerve section; group B was submitted to nerve fibular section only. The others groups had the nerve fibular sectioned and then repaired in the lateral surface of an intact tibial nerve, with different procedures: group C: ETS with sutures; group D: ETS with sutures and NGF; group E: ETS with FG only; group F: ETS with FG containing NGF. The motor function was accompanied and the tibial muscle mass, the number and diameter of muscular fibers and regenerated axons were measured. RESULTS: All the analyzed variables did not show any differences among the four operated groups (p>0.05), which were statistically superior to group B (p<0.05), but inferior to group A (p>0.05). CONCLUSION: The end-to-side nerve repair presented the same recovery pattern, independent from the repair used, showing that the addition of nerve growth factor in fibrin glue was not enough for the results potentiating.

Effects and Mechanism of Mouse Nerve Growth Factor on Diffuse Brain Injury in Rats with Traumatic Brain Injury Combined with Seawater Drowning

2021 ◽  
Vol 11 (12) ◽  
pp. 2321-2328
Author(s):  
Zihuan Zeng ◽  
Hao Zhang ◽  
Jianwu Wu ◽  
Liangfeng Wei ◽  
Weiqiang Chen ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Nerve Growth Factor ◽  
Brain Injury ◽  
Growth Factor ◽  
Cell Swelling ◽  
Nerve Growth ◽  
Diffuse Brain Injury ◽  
Severity Scores ◽  
Group A ◽  
Group B

To investigate the effect of mouse nerve growth factor (mNGF) on axonal injury after traumatic brain injury (TBI) combined with seawater drowning (SWD) in rats and the possible mechanism, we assigned 78 SD rats by random into sham group (Group A, n = 14), TBI+SWD group (Group B, n = 32), and mNGF group (Group C, n = 32). The compound injury model of rats was established by Marmarou method (450 g×1.5 m) and intratracheal pumping seawater (3 ml/kg). Rats in Group C were subject to intraperitoneal injection of mNGF (3 ug/kg) immediately after injury, and Group A as well as Group B were intraperitoneally injected the same amount of normal saline. Modified neurological severity scores(mNSS) was performed on rats at 12 h, 24 h, 72 h as well as 168 h, respectively after injury. HE staining showed that 24 h after injury, the swelling of nerve cells in Group C was relatively milder and the tissue edema was similar to that in Group B. At 72 h and 168 h after injury, the matrix looseness, cell swelling, and nuclear condensation in Group C were improved in comparison with Group B. (2) Compared with group B, mNSS of group C decreased signally at 72 h and 68 h after injury (P <0.05). (3) IHC staining showed that the protein expressions of β-APP, NF-L, and AQP4 were decreased in Group C in comparison with Group B at 12 h, 24 h, 72 h and 168 h after injury. (4) Brain water content in Group C was similar to that in Group B (P >0.05). (5) At 12 h after injury, the expression of TNF-α in Group C was signally lower than that in Group B (P < 0.05). Our reseache showed that mNGF might play a neuroprotective role by reducing cerebral edema and inflammatory response after TBI+SWD injury in rats, thereby restoring part of the injured axons in TBI+SWD rats.

scholarly journals End-to-side nerve repair using fibrin glue in rats

2010 ◽  
Vol 25 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Daniel Nunes e Silva ◽  
Julice Coelho ◽  
Fabrício de Oliveira Frazílio ◽  
Alexandre Nakao Odashiro ◽  
Paulo de Tarso Camillo de Carvalho ◽  
...  
Keyword(s):  
Fibrin Glue ◽  
Axonal Regeneration ◽  
Nerve Repair ◽  
Control Group ◽  
Morphometrical Analysis ◽  
Walking Pattern ◽  
Group A ◽  
Nerve Anastomosis ◽  
Group B ◽  
Control Group Group

PURPOSE: To evaluate axonal regeneration after end-to-side nerve repair with fibrin glue in rats. METHODS: Forty-five Wistar rats were divided into three groups: group A (n=15), were not submitted to surgery (control group); group B (n=15) were submitted to fibular transection without repair; and group C (n=15), were submitted to fibular transection with end-to-side nerve anastomosis using fibrin glue, in the lateral surface of an intact tibial nerve. The three groups were submitted to walking track (30 and 90 days) and posterior morphometrical analysis (90 days). RESULTS: The functional tests demonstrated that there was no difference in the walking track during the study in group A (p>0.05). The group B had walking pattern impairment in the two tests (p>0.05). The group C had walking pattern impairment in the first test, with important recovery in the second test (p<0.05). The morphometrical assessment revealed significantly higher number of regenerated mielinates axons in group C, compared to group B (p<0.05). CONCLUSION: The end-to-side nerve repair with fibrin glue shows axonal recovery, demonstrated through functional and morphometrical ways in rats.

Nerve growth factor counteracts the neurophysiological and neurochemical effects of chronic sciatic nerve section

1985 ◽  
Vol 332 (1) ◽  
pp. 131-141 ◽  
Author(s):  
M. Fitzgerald ◽  
P.D. Wall ◽  
M. Goedert ◽  
P.C. Emson
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sciatic Nerve ◽  
Nerve Section ◽  
Nerve Growth

scholarly journals Electrophysiologic assessment of regeneration in rat sciatic nerve repair using suture, fibrin glue or a combination of both techniques

2005 ◽  
Vol 63 (3a) ◽  
pp. 601-604 ◽  
Author(s):  
Roberto Sergio Martins ◽  
Mario Gilberto Siqueira ◽  
Ciro Ferreira da Silva ◽  
Benedito Ortiz de Godoy ◽  
José Píndaro Pereira Plese
Keyword(s):  
Sciatic Nerve ◽  
Action Potential ◽  
Fibrin Glue ◽  
Conduction Velocity ◽  
Nerve Section ◽  
Motor Action ◽  
Rat Sciatic Nerve ◽  
Nerve Action ◽  
Group A ◽  
Group B

We evaluated the repair of seccioned rat sciatic nerve by the comparison of electrophysiologic parameters. The repair was effected with suture (group A), fibrin glue (group B) or a combination of both techniques (group C). The amplitude, latency and conduction velocity of the motor and nerve action potentials were assessed before the nerve section and at reoperation after 24 weeks. There was no difference between the groups when the nerve action potential was evaluated. Rats of group B presented better results than those of group A (p<0.05) when latency and the nerve conduction velocity assessed at the reoperation, and the ratio between the conduction velocity at the reoperation and before the nerve section in the motor action potential evaluation were measured. Animals of group C presented better results than those of group A when the ratio between the conduction velocity of motor action potential at the reoperation and before the nerve division was considered (p<0.05). No difference between groups B and C was found. We conclude that repair using fibrin glue presented better results than suture following transection of sciatic nerve when the motor action potential was evaluated in the rat experimental model.

Siatic nerve regeneration in rats stimulated by fibrin glue containing nerve growth factor: An experimental study

Injury ◽  
2008 ◽  
Vol 39 (12) ◽  
pp. 1414-1420 ◽  
Author(s):  
Gao Chunzheng ◽  
Ma Shengzhong ◽  
Ji Yinglian ◽  
Wang Ji-e ◽  
Li Jianmin
Keyword(s):  
Experimental Study ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Regeneration ◽  
Fibrin Glue ◽  
Nerve Growth

scholarly journals Nerve Growth Factor Changes and Corneal Nerve Repair after Keratoplasty

2018 ◽  
Vol 95 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Yueji Pan ◽  
Fang Liu ◽  
Xiaofeng Qi ◽  
Yuan Hu ◽  
Feihong Xu ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Repair ◽  
Nerve Growth ◽  
Corneal Nerve
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