scholarly journals Effect of instrumentation using curettes, piezoelectric ultrasonic scaler and Er,Cr:YSGG laser on the morphology and adhesion of blood components on root surfaces: a SEM study

2011 ◽  
Vol 22 (3) ◽  
pp. 185-192 ◽  
Author(s):  
Jackeline do Nascimento Tsurumaki ◽  
Bráulio Henrique Marques Souto ◽  
Guilherme José Pimentel Lopes de Oliveira ◽  
José Eduardo Cézar Sampaio ◽  
Elcio Marcantonio Júnior ◽  
...  

This study used scanning electron microscopy (SEM) to evaluate the morphology and adhesion of blood components on root surfaces instrumented by curettes, piezoelectric ultrasonic scaler and Er,Cr:YSGG laser. One hundred samples from 25 teeth were divided into 5 groups: 1) Curettes; 2) Piezoelectric ultrasonic scaler; 3) Curettes plus piezoelectric ultrasonic scaler; 4) Er,Cr:YSGG laser; 5) Curettes plus Er,Cr:YSGG laser. Ten samples from each group were used for analysis of root morphology and the other 10 were used for analysis of adhesion of blood components on root surface. The results were analyzed statistically by the Kruskall-Wallis and Mann-Whitney tests with a significance level of 5%. The group treated with curettes showed smoother surfaces when compared to the groups were instrumented with piezoelectric ultrasonic scaler and the Er,Cr:YSGG laser. The surfaces instrumented with piezoelectric ultrasonic scaler and Er,Cr:YSGG laser, alone or in combination with hand scaling and root planing, did not differ significantly (p>0.05) among themselves. No statistically significant differences (p>0.05) among groups were found as to the adhesion of blood components on root surface. Ultrasonic instrumentation and Er,Cr:YSGG irradiation produced rougher root surfaces than the use of curettes, but there were no differences among treatments with respect to the adhesion of blood components.

2002 ◽  
Vol 16 (4) ◽  
pp. 308-312 ◽  
Author(s):  
Letícia Helena Theodoro ◽  
Valdir Gouveia Garcia ◽  
Patrícia Haypek ◽  
Denise Maria Zezell ◽  
Carlos de Paula Eduardo

The purpose of this study was to morphologically evaluate, by means of scanning electron microscopy, the effects of Er:YAG laser on the treatment of root surfaces submitted to scaling and root planing with conventional periodontal instruments. Eighteen root surfaces (n = 18), which had been previously scaled and planed, were assigned to 3 groups (n = 6). The control Group (G1) received no further treatment; Group 2 (G2) was irradiated with Er:YAG laser (2.94 mum), with 47 mJ/10 Hz, in a focused mode with air/water spray during 15 s and with 0.57 J/cm² of fluency per pulse; Group 3 (G 3) was irradiated with Er:YAG laser (2.94 mum), with 83 mJ/10 Hz, in a focused mode with air/water spray during 15 s and with 1.03 J/cm² of fluency per pulse. We concluded that the parameters adopted for Group 3 removed the smear layer from the root surface, exposing the dentinal tubules. Although no fissures, cracks or carbonized areas were observed, an irregular surface was produced by Er:YAG laser irradiation. Thus, the biocompatibility of the irradiated root surface, within the periodontal healing process, must be assessed.


2016 ◽  
Vol 48 (3) ◽  
pp. 388 ◽  
Author(s):  
S. Karschová ◽  
J. Hajer

The spinning apparatus and silk of <em>Segestria senoculata</em> were studied with the use of scanning electron microscopy and light microscopy, which confirmed the presence of four categories of spigots connected with four types of spinning glands (<em>i.e</em>., <em>Glandulae ampullaceae major, Glandulae ampullaceae minor, Glandulae piriformes and Glandulae pseudaciniformes</em>). New data about the morphology of spinnerets and spigots were obtained for both the adults and nymphal stages of both sexes. For the first time the silken threads of retreats, signal threads and attachment discs of the members of <em>Segestria</em> were subjected to a detailed SEM study. The data resulting from studying the spinning apparatus of <em>S. senoculata</em> was compared to current knowledge of the silk producing systems of families belonging to the Dysderoidea superfamily. Silks that are emitted from spigots in the course of retreat construction are not (similarly to the other dysderoids) processed by the spider’s legs during the subsequent process of hardening. Apart from the major ampullate glands/spigots, segestriids also possess developed minor ampullate glands. Minor ampullate threads are used by <em>S. senoculata</em> spiders when making their signal threads.


Weed Science ◽  
1978 ◽  
Vol 26 (6) ◽  
pp. 619-625 ◽  
Author(s):  
R. E. Hoagland ◽  
R. N. Paul

A comparative scanning electron microscopy (SEM) study was performed on the surfaces of seeds and coleoptiles of several conspecific rice(Oryza sativaL.) varieties including the weed red rice and several cultivars: Mochi-Gommi and LA-110 (medium grained); Starbonnet, Labelle, and Bluebelle (long grained). Seed surfaces of red rice, LA-110 and Mochi-Gommi possessed trichomes that were more numerous at the seed apex than on other seed parts. Red rice also exhibited a large central awn extending from the seed apex. This awn had spines along its axis and numerous spines at its apical end. These characteristics were absent in the other varieties. No significant differences were noted in the seed-surface tubercles and in the quantity or structure of wax on the seed surface in all six samples. The coleoptiles of all samples had several similarities: the stomata were arranged in parallel rows on both the adaxial and abaxial surfaces, the epicuticular wax structure on both surfaces was rodlet, prickle hairs were found on the adaxial coleoptile surface (but absent on abaxial), spines were present on the edge of the apical end (older portion) and decreased in number toward the basal end (younger portion), and two types of papillae (based on size and shape) were present on both the adaxial and abaxial surfaces. Although there were varietal differences among developing coleoptiles on a macroscopic scale, significant morphological differences were not discernible at the SEM level.


2019 ◽  
Vol 48 ◽  
Author(s):  
Luis Gustavo Gonzalez OSUNA ◽  
Guilherme José Pimentel Lopes de OLIVEIRA ◽  
Lucas Henrique de Souza TEIXEIRA ◽  
Claudia Oliveira MARQUEZ ◽  
Milena Suemi IRIE ◽  
...  

Abstract Introduction Root-scaling procedures on the root surface show to increase root surface roughness during instrumentation. The use of a prophylactic paste reduces the roughness. Objetive This study evaluated the influence of prophylaxis paste on the surface roughness of root dentin after hand instruments (HI), ultrasonic scalers (US) and rotational instruments (RI) were applied to root surfaces. Material and method Fifty bovine roots were prepared and randomly divided into 10 groups (n=5) in which instrumentation with HI, US, or RI was carried out with or without prophylaxis using either conventional or 3% hydroxyapatite paste; additionally, a control group without treatment was included. The Ra and Rz roughness values were measured using a perfilometer. The morphological aspects of the root surfaces were analysed qualitatively by scanning electron microscopy (SEM). Data were analysed using two-way ANOVA followed by Tukey’s test (α = 0.05). Result The scaling method and the prophylaxis procedure significantly influenced (p < 0.05) root surface roughness. Treatment with US and prophylaxis with 3% hydroxyapatite paste resulted in lower values of Ra (0.14 μm ± 0.02 μm) and Rz (0.89 μm ± 0.18 μm) compared to the other groups. SEM analysis showed smoother and more homogeneous surfaces in the groups with prophylaxis compared to the groups without prophylaxis. There were no substantial differences between the results of applying different pastes. Conclusion The prophylaxis procedure performed with the experimental paste containing 3% hydroxyapatite reduced the roughness of root surfaces treated with HI, US and RI at the same level as the conventional prophylactic paste.


2012 ◽  
Vol 23 (5) ◽  
pp. 502-507 ◽  
Author(s):  
Piero Rocha Zanardi ◽  
Bruno Costa ◽  
Roberto Chaib Stegun ◽  
Newton Sesma ◽  
Matsuyoshi Mori ◽  
...  

The present study evaluated the interchangeability of prosthetic components for external hexagon implants by measuring the precision of the implant/abutment (I/A) interface with scanning electron microscopy. Ten implants for each of three brands (SIN, Conexão, Neodent) were tested with their respective abutments (milled CoCr collar rotational and non-rotational) and another of an alternative manufacturer (Microplant) in randomly arranged I/A combinations. The degree of interchangeability between the various brands of components was defined using the original abutment interface gap with its respective implant as the benchmark dimension. Accordingly, when the result for a given component placed on an implant was equal to or smaller then that gap measured when the original component of the same brand as the implant was positioned, interchangeability was considered valid. Data were compared with the Kruskal-Wallis test at 5% significance level. Some degree of misfit was observed in all specimens. Generally, the non-rotational component was more accurate than its rotational counterpart. The latter samples ranged from 0.6-16.9 µm, with a 4.6 µm median; and the former from 0.3-12.9 µm, with a 3.4 µm median. Specimens with the abutment and fixture from Conexão had larger microgap than the original set for SIN and Neodent (p<0.05). Even though the latter systems had similar results with their respective components, their interchanged abutments did not reproduce the original accuracy. The results suggest that the alternative brand abutment would have compatibility with all systems while the other brands were not completely interchangeable.


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