scholarly journals Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe

2001 ◽  
Vol 44 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Marcia O. Mello ◽  
Murilo Melo ◽  
Beatriz Appezzato-da-Glória
Keyword(s):  
Liquid Medium ◽  
Histological Analysis ◽  
Adventitious Shoots ◽  
Naphthaleneacetic Acid ◽  
Curcuma Zedoaria ◽  
Shoot Bud ◽  
Skoog Medium ◽  
Root Segments ◽  
Murashige And Skoog Medium

Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed on agar-solidified Murashige and Skoog medium without growth regulators at 25º C in the light. The establishment of these plantlets in soil was about 95%.

scholarly journals 880 PB 270 In Vitro Flower and Plant Formation from Opuntia dillenii Haw. and Opuntia cochenillifera Mill.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560a-560
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Fresh Medium ◽  
Naphthaleneacetic Acid ◽  
Forage Crop ◽  
Skoog Medium ◽  
Number Of Species ◽  
Murashige And Skoog Medium ◽  
Opuntia Dillenii

The genus Opuntia includes a number of species which produce nutritious fruits and edible young joints, moreover, they are used as forage crop and for medicinal purposes. A procedure for flower and plant formation is described. Explants were excised from young flower receptacle and cultured on Murashige and Skoog medium (MS) supplemented with 0.9, 1.8, 4.5, or 9 uM thidiazuron and 0.5 uM naphthaleneacetic acid. Produced shoots were bisected longitudinally and cultured in a fresh medium from the same composition, other half shoots were subjected to a second cut by dividing transversely into distal and proximal explants before culture. About 80% explants of O. dellini produced flowers which produced shoots form the distal ales. only shoots were formed from O. cochenillifera explants. The distal explants produced more shoots than proximal explants. All formed shoots which attained 3 mm in length or longer were rooted directly in soil and all shoots formed roots and produced normal plants.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

In vitro propagation of Crambe maritima

1987 ◽  
Vol 65 (1) ◽  
pp. 72-75 ◽  
Author(s):  
J. Y. Peron ◽  
E. Regnier
Keyword(s):  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Petiole Explants ◽  
Acclimatization Period

A method for rapid micropropagation of sea kale (Crambe maritima L.) was developed. Petiole explants placed in vitro on a medium containing 0.5 mg/L indoleacetic acid (IAA), 6.0 mg/L kinetin, and 1.5 mg/L benzylaminopurine developed callus within 15 days and shoots within 28 days. Nearly four adventitious shoots could be developed within 3 weeks by placing the initial shoot on media without IAA. To develop roots, the shoots were then transferred to the basal medium containing 0.1 to 1.0 mg/L indolbutyric or α-naphthaleneacetic acid. Rooted plantlets were obtained within 2 or 3 weeks. After an acclimatization period of 6 weeks in a greenhouse in unsterilized medium, the plantlets could be set outdoors.

Regeneration of peanut (Arachis hypogaea) plantlets by in vitro culture of immature leaves

1981 ◽  
Vol 59 (5) ◽  
pp. 826-830 ◽  
Author(s):  
L. A. Mroginski ◽  
K. K. Kartha ◽  
J. P. Shyluk
Keyword(s):  
In Vitro Culture ◽  
Arachis Hypogaea ◽  
Environmental Conditions ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Bud Regeneration ◽  
Arachis Hypogaea L ◽  
Murashige And Skoog Medium ◽  
Germinated Seeds

The in vitro regeneration of buds, shoots, and roots from immature leaves of 3- to 5-day-old peanut (Arachis hypogaea L. cv. Colorado Manfredi) seedlings was studied under defined nutritional, hormonal, and environmental conditions. The first two leaves (2–5 mm in length) removed from aseptically germinated seeds were cultured on Murashige and Skoog medium containing vitamins as in B5 medium and 0.8% agar, supplemented with 12 combinations of naphthaleneacetic acid (NAA) (0.01 to 4 mg/L) and benzyladenine (BA) (1 and 3 mg/L). Bud regeneration occurred in all hormone combinations, but the maximum number of buds was regenerated at a concentration of 1 mg/L each of NAA and BA. Although bud regeneration was maximum with 2- to 5-mm-long leaflets, some success was also obtained with leaflets 8–13 mm long. However, no buds were regenerated when fully expanded leaflets were cultured.Development of buds into shoots was readily achieved by transferring regenerated buds into fresh medium containing 0.01 mg/L NAA and 1 mg/L BA. A few roots were induced to grow when callus with buds was also transferred to medium devoid of hormones. So far, bud regeneration from immature leaves has been induced in vitro in 5 of the 10 cultivars tested.

scholarly journals Micropropagation and callogenesis of Curcuma zedoaria Roscoe

2004 ◽  
Vol 61 (4) ◽  
pp. 427-432 ◽  
Author(s):  
Jeanette Inamine Miachir ◽  
Vera Lúcia Moretti Romani ◽  
Antônio Francisco de Campos Amaral ◽  
Marcia Ometto Mello ◽  
Otto Jesu Crocomo ◽  
...  
Keyword(s):  
Growth And Development ◽  
Cell Suspension Cultures ◽  
Culture Conditions ◽  
Plant Production ◽  
Curcuma Zedoaria ◽  
Plant Growth And Development ◽  
Skoog Medium ◽  
Medicinal Properties ◽  
Benzyl Amino Purine

Curcuma zedoaria Roscoe (zedoary) is a medicinal properties-bearing Zingiberaceae from which rhizomes are commercially exploited. The objective of this work was to establish an in vitro protocol for micropropagation and callogenesis of Curcuma zedoaria Roscoe as alternative to improve plant production, turning economically feasible the exploitation of its secondary metabolites which present medicinal properties. Micropropagation by using shoot apexes produced by rhizome and from in vitro plants were carried out on Murashige & Skoog medium supplemented with 2.0 mg L-1 benzyl amino purine and 30 g L-1 sucrose. Plantlets were satisfactorily acclimated to greenhouse conditions by using plastic cover for at least 10 days. Treatment with endomycorrhiza at the ex vitro transferring time was beneficial to acclimatization, improving plant growth and development. Callus induction and growth were obtained by inoculating root segments on Murashige & Skoog medium supplemented with 1.0 mg L-1 naphtalene acetic acid and incubation in the dark at 25 ± 2ºC. Cell suspension cultures were established on liquid medium of same chemical composition and same culture conditions and a growth curve was obtained.

REGENERATION OF TREMA ORIENTALIS (BLUME) LINN.: EFFECT OF GROWTH REGULATORS, CULTURE CONDITIONS, AND AGE AND SOURCE OF EXPLANTS

1995 ◽  
Vol 43 (4) ◽  
pp. 391-395 ◽  
Author(s):  
G.R. Rout ◽  
S. Samantaray ◽  
P. Das
Keyword(s):  
Growth Regulators ◽  
Leaf Explants ◽  
Naphthaleneacetic Acid ◽  
Mature Trees ◽  
Regeneration Rate ◽  
Bud Regeneration ◽  
Shoot Bud ◽  
Shoot Bud Regeneration ◽  
Trema Orientalis

Optimal conditions for high frequency shoot bud regeneration from leaf callus of Trema orientalis (Blume) Linn. were studied. The regeneration rate was controlled by the growth regulators, the age and the source of the explants, and the illumination conditions. Irrespective of illumination conditions, shoot bud regeneration was achieved only in media containing benzyladenine (BA) + α-naphthaleneacetic acid (NAA) combinations, with the best results being obtained in the presence of 2.5 mg/1 BA and 0.25–0.5 mg/1 NAA. The morphogenic response was less frequent in the calluses derived from leaf explants of the mature trees compared to those of the in vitro-grown seedlings. The rate of shoot bud regeneration was more pronounced in the cultures maintained for 4 weeks in the light (16-h photoperiod) than the cultures incubated in the dark. Regenerated shoots were rooted on the medium containing 1/2 strength basal Murashige and Skoog (MS) salts supplemented with 0.01 mg/1 NAA or indole-3-butyric acid (IBA). The rooted plantlets were established in the greenhouse.

ADVENTITIOUS SHOOTS FROM COTYLEDONS OF IMMATURE CHERRY AND APRICOT EMBRYOS

1986 ◽  
Vol 66 (4) ◽  
pp. 953-959 ◽  
Author(s):  
W. DAVID LANE ◽  
F. COSSIO
Keyword(s):  
Sweet Cherry ◽  
Prunus Persica ◽  
Immature Embryo ◽  
Adventitious Shoots ◽  
Prunus Armeniaca ◽  
Mature Embryos ◽  
Stage Of Development ◽  
Physiological Stage ◽  
Murashige And Skoog Medium

Immature embryos of Prunus armeniaca (apricot) and Prunus persica (peach) collected 20–30 d from anthesis were cultured on Murashige and Skoog medium supplemented with benzyladenine (BA) and various auxins to study their potential for regeneration. Both species developed adventitious buds on cotyledons when cultured in vitro. Apricot frequency of regeneration was as high as 100% when BA (5.0 μM) and 2,4-D (1.0 μM) were included in the medium. Cherry response was less than apricot (up to 70%) and the maximum frequency of regeneration occurred using media with BA alone (3.0 μM). Auxin was inhibitory to sweet cherry regeneration. The physiological stage of development was very important for regeneration from both species since regeneration did not occur when very young or fully mature embryos were used as explants. Apricot plants were produced by rooting shoots which developed from the regenerated buds on the cotyledons.Key words: Apricot, sweet cherry, regeneration, immature embryo, cotyledon, tissue culture

scholarly journals Effects of Cytokinin on in vitro Propagation of Bauhinia variegata L.

2020 ◽  
Vol 1 (6) ◽  
Author(s):  
Belai Meeta Suwal Singh
Keyword(s):  
In Vitro Propagation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Nodal Cuttings ◽  
Half Strength ◽  
Bauhinia Variegata ◽  
Mature Seeds

Mature seeds of Bauhinia variegata L were cultured on half strength Murashige and Skoog medium. For experimentation, nodal cuttings were used as explants from in vitro growing plants. Cytokinin, N-benzyl-9-(2-tetrahydropyranyl) (BPA), kinetin(6-furfurylaminopurine), zeatin, 6-(4-hydroxy-3-methyl-trans -2-butenyl amino purine), 2- isopentenyl amino purine (2-ip), and benzylaminopurine (BAP) were tested for best propagation. Well grown plants were achieved in medium supplemented with 5 µM BPA and 0.5 µM BAP. The propagated plants were acclimatized very well after transferred to the field.

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