scholarly journals 880 PB 270 In Vitro Flower and Plant Formation from Opuntia dillenii Haw. and Opuntia cochenillifera Mill.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560a-560
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Fresh Medium ◽  
Naphthaleneacetic Acid ◽  
Forage Crop ◽  
Skoog Medium ◽  
Number Of Species ◽  
Murashige And Skoog Medium ◽  
Opuntia Dillenii

The genus Opuntia includes a number of species which produce nutritious fruits and edible young joints, moreover, they are used as forage crop and for medicinal purposes. A procedure for flower and plant formation is described. Explants were excised from young flower receptacle and cultured on Murashige and Skoog medium (MS) supplemented with 0.9, 1.8, 4.5, or 9 uM thidiazuron and 0.5 uM naphthaleneacetic acid. Produced shoots were bisected longitudinally and cultured in a fresh medium from the same composition, other half shoots were subjected to a second cut by dividing transversely into distal and proximal explants before culture. About 80% explants of O. dellini produced flowers which produced shoots form the distal ales. only shoots were formed from O. cochenillifera explants. The distal explants produced more shoots than proximal explants. All formed shoots which attained 3 mm in length or longer were rooted directly in soil and all shoots formed roots and produced normal plants.

scholarly journals Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe

2001 ◽  
Vol 44 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Marcia O. Mello ◽  
Murilo Melo ◽  
Beatriz Appezzato-da-Glória
Keyword(s):  
Liquid Medium ◽  
Histological Analysis ◽  
Adventitious Shoots ◽  
Naphthaleneacetic Acid ◽  
Curcuma Zedoaria ◽  
Shoot Bud ◽  
Skoog Medium ◽  
Root Segments ◽  
Murashige And Skoog Medium

Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed on agar-solidified Murashige and Skoog medium without growth regulators at 25º C in the light. The establishment of these plantlets in soil was about 95%.

scholarly journals 879 PB 267 Micropropagation of Grand Crinum Lily (Crinum asiaticum L.)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 559g-559
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Multiple Shoots ◽  
Ornamental Plant ◽  
White Flower ◽  
Fresh Medium ◽  
Naphthaleneacetic Acid ◽  
Plant Propagation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Crinum Asiaticum

Grand crinum Lily is an ornamental plant which is considered to be one of the architect's favorite accent. plants and one that bloom most of the year. A protocol for plant propagation from inflorescence is described. Explants were excised from inflorescence at the primordial stage. and cultured on Murashige and Skoog medium (MS: alone or supplemented with benzyladenine 4.4 or 13.3 uM benzyladenine (BA) and 0.5 uM naphthaleneacetic acid (NAA) and incubated for four weeks. Explants cultured on BA-containing media produced white flower-like structures on the receptacle which produced multiple shoots after additional four weeks on a fresh medium containing 4.4 uM BA and 0.05 uM NAA. Shoots were transferred to a fresh medium for further growth during which the basal stem reached 3 to 5 mm diameter. At this stage shoots, with or without roots, were transferred to soil, without acclimatization, and normal plants were established in soil.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

Regeneration of peanut (Arachis hypogaea) plantlets by in vitro culture of immature leaves

1981 ◽  
Vol 59 (5) ◽  
pp. 826-830 ◽  
Author(s):  
L. A. Mroginski ◽  
K. K. Kartha ◽  
J. P. Shyluk
Keyword(s):  
In Vitro Culture ◽  
Arachis Hypogaea ◽  
Environmental Conditions ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Bud Regeneration ◽  
Arachis Hypogaea L ◽  
Murashige And Skoog Medium ◽  
Germinated Seeds

The in vitro regeneration of buds, shoots, and roots from immature leaves of 3- to 5-day-old peanut (Arachis hypogaea L. cv. Colorado Manfredi) seedlings was studied under defined nutritional, hormonal, and environmental conditions. The first two leaves (2–5 mm in length) removed from aseptically germinated seeds were cultured on Murashige and Skoog medium containing vitamins as in B5 medium and 0.8% agar, supplemented with 12 combinations of naphthaleneacetic acid (NAA) (0.01 to 4 mg/L) and benzyladenine (BA) (1 and 3 mg/L). Bud regeneration occurred in all hormone combinations, but the maximum number of buds was regenerated at a concentration of 1 mg/L each of NAA and BA. Although bud regeneration was maximum with 2- to 5-mm-long leaflets, some success was also obtained with leaflets 8–13 mm long. However, no buds were regenerated when fully expanded leaflets were cultured.Development of buds into shoots was readily achieved by transferring regenerated buds into fresh medium containing 0.01 mg/L NAA and 1 mg/L BA. A few roots were induced to grow when callus with buds was also transferred to medium devoid of hormones. So far, bud regeneration from immature leaves has been induced in vitro in 5 of the 10 cultivars tested.

scholarly journals Effects of Cytokinin on in vitro Propagation of Bauhinia variegata L.

2020 ◽  
Vol 1 (6) ◽  
Author(s):  
Belai Meeta Suwal Singh
Keyword(s):  
In Vitro Propagation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Nodal Cuttings ◽  
Half Strength ◽  
Bauhinia Variegata ◽  
Mature Seeds

Mature seeds of Bauhinia variegata L were cultured on half strength Murashige and Skoog medium. For experimentation, nodal cuttings were used as explants from in vitro growing plants. Cytokinin, N-benzyl-9-(2-tetrahydropyranyl) (BPA), kinetin(6-furfurylaminopurine), zeatin, 6-(4-hydroxy-3-methyl-trans -2-butenyl amino purine), 2- isopentenyl amino purine (2-ip), and benzylaminopurine (BAP) were tested for best propagation. Well grown plants were achieved in medium supplemented with 5 µM BPA and 0.5 µM BAP. The propagated plants were acclimatized very well after transferred to the field.

scholarly journals Вплив стимуляторів росту на ініціацію експлантів Thuja Occidentalis L. в умовах in vitro

2019 ◽  
Vol 29 (5) ◽  
pp. 47-50
Author(s):  
M. M. Lisoviy
Keyword(s):  
Thuja Occidentalis ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

На основі критичного аналізу літературних джерел встановлено потребу проведення поглибленого дослідження процесу росту експлантів Thuja occidentalis L. на етапі ініціації під час мікроклонального розмноження в умовах in vitro під впливом різних стимуляторів росту. Розглянуто застосовану методику виконання експериментальних досліджень. Проведено тестування трьох рецептів базових живильних середовищ: Murashige and Skoog medium (MS), Risser and White medium (RW) та Litvay medium (LM) і встановлено, що для мікроклонування досліджуваного виду оптимальним є RW. Визначено вплив синтетичних фітогормонів ауксинового (2,4-D та НОК) і цитокінінового (БАП) типу на успішність проходження етапу ініціації експлантів Thuja occidentalis L. Досліджено явище утворення калюсної тканини в експлантів під впливом різних ростових речовин. Виконано якісну оцінку ініціації експлантів, на основі якої проведено їхній поділ на три групи: "незадовільна ініціація", "задовільна ініціація" та "добра ініціація", залежно від збільшення лінійних розмірів рослинного матеріалу. Встановлено, що оптимальним живильним середовищем для ініціації росту досліджуваного виду є RW, модифіковане 0,3 мг/л (2,4-D)+0,5 мг/л (НОК)+0,2 мг/л (БАП).

scholarly journals Axillary Shoot Proliferation from in Vitro Culture of Pulmonaria L. Shoots

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 630d-630
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Shoot Proliferation ◽  
Axillary Shoot ◽  
Optimum Level ◽  
Axillary Shoot Proliferation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Rooting Response

Actively growing shoots from Pulmonaria L. `Roy Davidson' were cultured in vitro on Murashige and Skoog medium containing benzyladenine (BA) to establish proliferating cultures. BA at 0, 0.4, 0.8, 4.4, 8.8, and 44.4 μm was compared for shoot proliferation and rooting response. Shoot count was highest on 8.8 μm BA with root count highest on 0 or 0.4 μm BA. Subculture 4 weeks later of shoots to the same treatments resulted in highest shoot counts on 44.4 μm BA. Optimum level for micropropagation was 8.8 or 44.4 μm BA. Greatest rooting was at 0 or 0.4 μm BA.

Sign in / Sign up

Export Citation Format

Share Document